Amino acid dipepetide frequency for Lactobacillus sanfranciscensis (strain TMW 1.1304)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.505AlaAla: 5.505 ± 0.151
0.311AlaCys: 0.311 ± 0.027
4.308AlaAsp: 4.308 ± 0.116
4.121AlaGlu: 4.121 ± 0.127
2.987AlaPhe: 2.987 ± 0.1
4.906AlaGly: 4.906 ± 0.126
1.205AlaHis: 1.205 ± 0.054
5.67AlaIle: 5.67 ± 0.155
5.795AlaLys: 5.795 ± 0.151
6.499AlaLeu: 6.499 ± 0.149
2.058AlaMet: 2.058 ± 0.075
3.645AlaAsn: 3.645 ± 0.107
1.882AlaPro: 1.882 ± 0.074
2.632AlaGln: 2.632 ± 0.096
2.456AlaArg: 2.456 ± 0.081
3.701AlaSer: 3.701 ± 0.093
4.086AlaThr: 4.086 ± 0.099
4.609AlaVal: 4.609 ± 0.133
0.509AlaTrp: 0.509 ± 0.037
2.358AlaTyr: 2.358 ± 0.081
0.0AlaXaa: 0.0 ± 0.0
Cys
0.317CysAla: 0.317 ± 0.032
0.065CysCys: 0.065 ± 0.012
0.233CysAsp: 0.233 ± 0.025
0.241CysGlu: 0.241 ± 0.026
0.241CysPhe: 0.241 ± 0.026
0.517CysGly: 0.517 ± 0.042
0.154CysHis: 0.154 ± 0.022
0.406CysIle: 0.406 ± 0.035
0.317CysLys: 0.317 ± 0.036
0.593CysLeu: 0.593 ± 0.045
0.095CysMet: 0.095 ± 0.016
0.265CysAsn: 0.265 ± 0.027
0.244CysPro: 0.244 ± 0.026
0.146CysGln: 0.146 ± 0.021
0.19CysArg: 0.19 ± 0.023
0.273CysSer: 0.273 ± 0.027
0.265CysThr: 0.265 ± 0.028
0.317CysVal: 0.317 ± 0.029
0.07CysTrp: 0.07 ± 0.015
0.176CysTyr: 0.176 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
3.961AspAla: 3.961 ± 0.129
0.333AspCys: 0.333 ± 0.032
4.024AspAsp: 4.024 ± 0.135
4.373AspGlu: 4.373 ± 0.127
2.954AspPhe: 2.954 ± 0.084
3.777AspGly: 3.777 ± 0.104
1.262AspHis: 1.262 ± 0.062
4.086AspIle: 4.086 ± 0.123
4.232AspLys: 4.232 ± 0.111
5.689AspLeu: 5.689 ± 0.138
1.497AspMet: 1.497 ± 0.066
3.293AspAsn: 3.293 ± 0.109
2.139AspPro: 2.139 ± 0.079
2.721AspGln: 2.721 ± 0.08
2.015AspArg: 2.015 ± 0.078
2.976AspSer: 2.976 ± 0.093
2.735AspThr: 2.735 ± 0.086
4.227AspVal: 4.227 ± 0.132
0.653AspTrp: 0.653 ± 0.043
2.524AspTyr: 2.524 ± 0.086
0.0AspXaa: 0.0 ± 0.0
Glu
3.804GluAla: 3.804 ± 0.11
0.252GluCys: 0.252 ± 0.024
2.851GluAsp: 2.851 ± 0.089
3.312GluGlu: 3.312 ± 0.108
2.488GluPhe: 2.488 ± 0.087
2.597GluGly: 2.597 ± 0.101
1.159GluHis: 1.159 ± 0.059
5.22GluIle: 5.22 ± 0.123
5.35GluLys: 5.35 ± 0.133
5.83GluLeu: 5.83 ± 0.157
1.922GluMet: 1.922 ± 0.073
4.072GluAsn: 4.072 ± 0.107
1.587GluPro: 1.587 ± 0.071
2.456GluGln: 2.456 ± 0.092
2.367GluArg: 2.367 ± 0.098
2.851GluSer: 2.851 ± 0.088
3.352GluThr: 3.352 ± 0.102
3.834GluVal: 3.834 ± 0.121
0.487GluTrp: 0.487 ± 0.042
2.098GluTyr: 2.098 ± 0.076
0.0GluXaa: 0.0 ± 0.0
Phe
3.219PheAla: 3.219 ± 0.108
0.279PheCys: 0.279 ± 0.027
2.84PheAsp: 2.84 ± 0.097
2.48PheGlu: 2.48 ± 0.09
2.117PhePhe: 2.117 ± 0.101
3.452PheGly: 3.452 ± 0.108
0.78PheHis: 0.78 ± 0.047
3.377PheIle: 3.377 ± 0.116
3.544PheLys: 3.544 ± 0.099
3.926PheLeu: 3.926 ± 0.123
1.186PheMet: 1.186 ± 0.056
2.738PheAsn: 2.738 ± 0.086
1.573PhePro: 1.573 ± 0.067
1.373PheGln: 1.373 ± 0.062
1.232PheArg: 1.232 ± 0.062
3.062PheSer: 3.062 ± 0.119
2.429PheThr: 2.429 ± 0.082
3.276PheVal: 3.276 ± 0.099
0.504PheTrp: 0.504 ± 0.039
1.679PheTyr: 1.679 ± 0.072
0.0PheXaa: 0.0 ± 0.0
Gly
4.227GlyAla: 4.227 ± 0.127
0.382GlyCys: 0.382 ± 0.03
3.401GlyAsp: 3.401 ± 0.1
3.276GlyGlu: 3.276 ± 0.105
3.106GlyPhe: 3.106 ± 0.097
4.062GlyGly: 4.062 ± 0.149
1.408GlyHis: 1.408 ± 0.061
5.824GlyIle: 5.824 ± 0.127
5.445GlyLys: 5.445 ± 0.116
6.195GlyLeu: 6.195 ± 0.151
2.061GlyMet: 2.061 ± 0.08
3.114GlyAsn: 3.114 ± 0.088
1.505GlyPro: 1.505 ± 0.064
2.22GlyGln: 2.22 ± 0.078
2.448GlyArg: 2.448 ± 0.086
3.788GlySer: 3.788 ± 0.114
3.845GlyThr: 3.845 ± 0.106
4.73GlyVal: 4.73 ± 0.126
0.65GlyTrp: 0.65 ± 0.043
2.559GlyTyr: 2.559 ± 0.093
0.0GlyXaa: 0.0 ± 0.0
His
1.332HisAla: 1.332 ± 0.062
0.116HisCys: 0.116 ± 0.018
1.251HisAsp: 1.251 ± 0.058
1.24HisGlu: 1.24 ± 0.059
1.08HisPhe: 1.08 ± 0.051
1.5HisGly: 1.5 ± 0.065
0.661HisHis: 0.661 ± 0.045
1.294HisIle: 1.294 ± 0.065
1.235HisLys: 1.235 ± 0.052
1.974HisLeu: 1.974 ± 0.079
0.444HisMet: 0.444 ± 0.036
1.018HisAsn: 1.018 ± 0.056
0.994HisPro: 0.994 ± 0.052
1.032HisGln: 1.032 ± 0.054
0.804HisArg: 0.804 ± 0.043
1.145HisSer: 1.145 ± 0.053
0.977HisThr: 0.977 ± 0.051
1.273HisVal: 1.273 ± 0.056
0.192HisTrp: 0.192 ± 0.023
0.885HisTyr: 0.885 ± 0.05
0.0HisXaa: 0.0 ± 0.0
Ile
5.776IleAla: 5.776 ± 0.155
0.582IleCys: 0.582 ± 0.041
5.053IleAsp: 5.053 ± 0.126
4.56IleGlu: 4.56 ± 0.134
3.479IlePhe: 3.479 ± 0.119
5.535IleGly: 5.535 ± 0.145
1.397IleHis: 1.397 ± 0.063
6.111IleIle: 6.111 ± 0.176
6.555IleLys: 6.555 ± 0.16
7.197IleLeu: 7.197 ± 0.182
2.052IleMet: 2.052 ± 0.077
4.996IleAsn: 4.996 ± 0.12
2.96IlePro: 2.96 ± 0.101
2.613IleGln: 2.613 ± 0.088
2.618IleArg: 2.618 ± 0.084
5.291IleSer: 5.291 ± 0.135
4.522IleThr: 4.522 ± 0.122
5.275IleVal: 5.275 ± 0.136
0.631IleTrp: 0.631 ± 0.041
2.578IleTyr: 2.578 ± 0.081
0.0IleXaa: 0.0 ± 0.0
Lys
5.082LysAla: 5.082 ± 0.117
0.252LysCys: 0.252 ± 0.031
4.354LysAsp: 4.354 ± 0.121
4.858LysGlu: 4.858 ± 0.141
2.895LysPhe: 2.895 ± 0.098
3.818LysGly: 3.818 ± 0.109
1.584LysHis: 1.584 ± 0.066
6.266LysIle: 6.266 ± 0.143
6.515LysLys: 6.515 ± 0.177
7.284LysLeu: 7.284 ± 0.178
2.924LysMet: 2.924 ± 0.101
5.22LysAsn: 5.22 ± 0.134
2.613LysPro: 2.613 ± 0.09
3.899LysGln: 3.899 ± 0.122
3.731LysArg: 3.731 ± 0.109
4.021LysSer: 4.021 ± 0.109
4.508LysThr: 4.508 ± 0.118
5.164LysVal: 5.164 ± 0.104
0.644LysTrp: 0.644 ± 0.048
3.035LysTyr: 3.035 ± 0.098
0.0LysXaa: 0.0 ± 0.0
Leu
7.14LeuAla: 7.14 ± 0.148
0.487LeuCys: 0.487 ± 0.038
5.529LeuAsp: 5.529 ± 0.133
4.576LeuGlu: 4.576 ± 0.129
4.081LeuPhe: 4.081 ± 0.129
6.16LeuGly: 6.16 ± 0.145
1.725LeuHis: 1.725 ± 0.07
7.663LeuIle: 7.663 ± 0.223
7.617LeuLys: 7.617 ± 0.166
8.093LeuLeu: 8.093 ± 0.21
2.54LeuMet: 2.54 ± 0.092
5.692LeuAsn: 5.692 ± 0.12
3.802LeuPro: 3.802 ± 0.115
3.287LeuGln: 3.287 ± 0.099
3.241LeuArg: 3.241 ± 0.086
6.252LeuSer: 6.252 ± 0.16
5.608LeuThr: 5.608 ± 0.121
6.612LeuVal: 6.612 ± 0.149
0.734LeuTrp: 0.734 ± 0.048
2.705LeuTyr: 2.705 ± 0.105
0.0LeuXaa: 0.0 ± 0.0
Met
2.107MetAla: 2.107 ± 0.077
0.162MetCys: 0.162 ± 0.023
1.633MetAsp: 1.633 ± 0.066
1.468MetGlu: 1.468 ± 0.059
1.086MetPhe: 1.086 ± 0.058
1.687MetGly: 1.687 ± 0.067
0.485MetHis: 0.485 ± 0.036
2.277MetIle: 2.277 ± 0.084
2.483MetLys: 2.483 ± 0.089
2.499MetLeu: 2.499 ± 0.085
0.861MetMet: 0.861 ± 0.058
1.746MetAsn: 1.746 ± 0.067
1.051MetPro: 1.051 ± 0.055
1.11MetGln: 1.11 ± 0.06
1.007MetArg: 1.007 ± 0.05
1.752MetSer: 1.752 ± 0.065
1.847MetThr: 1.847 ± 0.075
1.92MetVal: 1.92 ± 0.076
0.206MetTrp: 0.206 ± 0.02
0.742MetTyr: 0.742 ± 0.049
0.0MetXaa: 0.0 ± 0.0
Asn
3.58AsnAla: 3.58 ± 0.103
0.303AsnCys: 0.303 ± 0.032
3.794AsnAsp: 3.794 ± 0.098
3.628AsnGlu: 3.628 ± 0.106
2.543AsnPhe: 2.543 ± 0.085
3.837AsnGly: 3.837 ± 0.103
1.552AsnHis: 1.552 ± 0.073
3.729AsnIle: 3.729 ± 0.102
4.17AsnLys: 4.17 ± 0.115
5.277AsnLeu: 5.277 ± 0.126
1.416AsnMet: 1.416 ± 0.063
3.349AsnAsn: 3.349 ± 0.128
2.697AsnPro: 2.697 ± 0.099
3.279AsnGln: 3.279 ± 0.124
2.299AsnArg: 2.299 ± 0.071
3.339AsnSer: 3.339 ± 0.111
2.426AsnThr: 2.426 ± 0.077
3.674AsnVal: 3.674 ± 0.096
0.696AsnTrp: 0.696 ± 0.044
2.339AsnTyr: 2.339 ± 0.085
0.0AsnXaa: 0.0 ± 0.0
Pro
2.404ProAla: 2.404 ± 0.082
0.1ProCys: 0.1 ± 0.016
2.285ProAsp: 2.285 ± 0.1
2.708ProGlu: 2.708 ± 0.091
1.722ProPhe: 1.722 ± 0.064
2.169ProGly: 2.169 ± 0.087
0.663ProHis: 0.663 ± 0.047
2.949ProIle: 2.949 ± 0.087
2.808ProLys: 2.808 ± 0.11
2.865ProLeu: 2.865 ± 0.09
0.804ProMet: 0.804 ± 0.04
2.031ProAsn: 2.031 ± 0.073
0.498ProPro: 0.498 ± 0.037
1.283ProGln: 1.283 ± 0.061
1.061ProArg: 1.061 ± 0.053
2.044ProSer: 2.044 ± 0.083
1.985ProThr: 1.985 ± 0.072
2.694ProVal: 2.694 ± 0.081
0.268ProTrp: 0.268 ± 0.024
1.243ProTyr: 1.243 ± 0.056
0.0ProXaa: 0.0 ± 0.0
Gln
3.006GlnAla: 3.006 ± 0.111
0.103GlnCys: 0.103 ± 0.02
1.711GlnAsp: 1.711 ± 0.07
1.882GlnGlu: 1.882 ± 0.071
1.7GlnPhe: 1.7 ± 0.069
2.147GlnGly: 2.147 ± 0.08
0.774GlnHis: 0.774 ± 0.048
3.712GlnIle: 3.712 ± 0.13
3.409GlnLys: 3.409 ± 0.087
4.021GlnLeu: 4.021 ± 0.115
1.205GlnMet: 1.205 ± 0.054
2.334GlnAsn: 2.334 ± 0.105
1.389GlnPro: 1.389 ± 0.071
2.031GlnGln: 2.031 ± 0.094
1.73GlnArg: 1.73 ± 0.086
2.215GlnSer: 2.215 ± 0.079
2.475GlnThr: 2.475 ± 0.087
2.843GlnVal: 2.843 ± 0.087
0.338GlnTrp: 0.338 ± 0.031
1.516GlnTyr: 1.516 ± 0.069
0.0GlnXaa: 0.0 ± 0.0
Arg
2.228ArgAla: 2.228 ± 0.077
0.181ArgCys: 0.181 ± 0.02
2.193ArgAsp: 2.193 ± 0.078
2.28ArgGlu: 2.28 ± 0.078
1.763ArgPhe: 1.763 ± 0.071
2.196ArgGly: 2.196 ± 0.092
0.839ArgHis: 0.839 ± 0.049
3.052ArgIle: 3.052 ± 0.095
3.084ArgLys: 3.084 ± 0.096
3.482ArgLeu: 3.482 ± 0.087
1.056ArgMet: 1.056 ± 0.052
2.052ArgAsn: 2.052 ± 0.085
1.332ArgPro: 1.332 ± 0.061
1.56ArgGln: 1.56 ± 0.076
1.887ArgArg: 1.887 ± 0.074
1.939ArgSer: 1.939 ± 0.081
1.844ArgThr: 1.844 ± 0.066
2.532ArgVal: 2.532 ± 0.093
0.265ArgTrp: 0.265 ± 0.026
1.459ArgTyr: 1.459 ± 0.062
0.0ArgXaa: 0.0 ± 0.0
Ser
3.766SerAla: 3.766 ± 0.106
0.238SerCys: 0.238 ± 0.029
3.628SerAsp: 3.628 ± 0.102
3.506SerGlu: 3.506 ± 0.097
2.905SerPhe: 2.905 ± 0.109
4.029SerGly: 4.029 ± 0.109
1.224SerHis: 1.224 ± 0.065
4.614SerIle: 4.614 ± 0.129
4.322SerLys: 4.322 ± 0.123
5.762SerLeu: 5.762 ± 0.119
1.622SerMet: 1.622 ± 0.069
3.241SerAsn: 3.241 ± 0.103
1.795SerPro: 1.795 ± 0.077
2.534SerGln: 2.534 ± 0.091
2.12SerArg: 2.12 ± 0.072
3.42SerSer: 3.42 ± 0.111
3.187SerThr: 3.187 ± 0.104
3.978SerVal: 3.978 ± 0.105
0.655SerTrp: 0.655 ± 0.043
2.15SerTyr: 2.15 ± 0.074
0.0SerXaa: 0.0 ± 0.0
Thr
3.75ThrAla: 3.75 ± 0.114
0.246ThrCys: 0.246 ± 0.028
3.455ThrAsp: 3.455 ± 0.099
3.29ThrGlu: 3.29 ± 0.098
2.551ThrPhe: 2.551 ± 0.096
4.113ThrGly: 4.113 ± 0.118
1.181ThrHis: 1.181 ± 0.057
4.633ThrIle: 4.633 ± 0.121
4.229ThrLys: 4.229 ± 0.104
4.969ThrLeu: 4.969 ± 0.112
1.373ThrMet: 1.373 ± 0.053
3.106ThrAsn: 3.106 ± 0.091
2.388ThrPro: 2.388 ± 0.088
1.714ThrGln: 1.714 ± 0.067
1.73ThrArg: 1.73 ± 0.069
3.325ThrSer: 3.325 ± 0.099
3.257ThrThr: 3.257 ± 0.089
3.97ThrVal: 3.97 ± 0.102
0.468ThrTrp: 0.468 ± 0.038
1.936ThrTyr: 1.936 ± 0.072
0.0ThrXaa: 0.0 ± 0.0
Val
5.315ValAla: 5.315 ± 0.133
0.428ValCys: 0.428 ± 0.034
4.243ValAsp: 4.243 ± 0.118
3.894ValGlu: 3.894 ± 0.116
2.976ValPhe: 2.976 ± 0.087
4.692ValGly: 4.692 ± 0.137
1.208ValHis: 1.208 ± 0.053
5.824ValIle: 5.824 ± 0.137
4.858ValLys: 4.858 ± 0.112
6.244ValLeu: 6.244 ± 0.14
1.933ValMet: 1.933 ± 0.084
3.68ValAsn: 3.68 ± 0.097
2.513ValPro: 2.513 ± 0.085
2.258ValGln: 2.258 ± 0.081
2.277ValArg: 2.277 ± 0.085
4.53ValSer: 4.53 ± 0.117
4.091ValThr: 4.091 ± 0.112
5.109ValVal: 5.109 ± 0.137
0.55ValTrp: 0.55 ± 0.041
2.145ValTyr: 2.145 ± 0.081
0.0ValXaa: 0.0 ± 0.0
Trp
0.436TrpAla: 0.436 ± 0.035
0.07TrpCys: 0.07 ± 0.015
0.452TrpAsp: 0.452 ± 0.042
0.398TrpGlu: 0.398 ± 0.033
0.514TrpPhe: 0.514 ± 0.038
0.552TrpGly: 0.552 ± 0.043
0.219TrpHis: 0.219 ± 0.026
0.872TrpIle: 0.872 ± 0.062
0.582TrpLys: 0.582 ± 0.036
1.113TrpLeu: 1.113 ± 0.058
0.292TrpMet: 0.292 ± 0.031
0.52TrpAsn: 0.52 ± 0.041
0.23TrpPro: 0.23 ± 0.028
0.371TrpGln: 0.371 ± 0.032
0.382TrpArg: 0.382 ± 0.034
0.566TrpSer: 0.566 ± 0.041
0.431TrpThr: 0.431 ± 0.036
0.52TrpVal: 0.52 ± 0.038
0.103TrpTrp: 0.103 ± 0.016
0.417TrpTyr: 0.417 ± 0.036
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.285TyrAla: 2.285 ± 0.071
0.233TyrCys: 0.233 ± 0.025
2.231TyrAsp: 2.231 ± 0.083
1.801TyrGlu: 1.801 ± 0.068
1.879TyrPhe: 1.879 ± 0.083
2.499TyrGly: 2.499 ± 0.08
0.969TyrHis: 0.969 ± 0.047
2.201TyrIle: 2.201 ± 0.083
2.117TyrLys: 2.117 ± 0.08
4.032TyrLeu: 4.032 ± 0.109
0.783TyrMet: 0.783 ± 0.042
1.817TyrAsn: 1.817 ± 0.084
1.394TyrPro: 1.394 ± 0.06
2.023TyrGln: 2.023 ± 0.081
1.63TyrArg: 1.63 ± 0.066
2.207TyrSer: 2.207 ± 0.076
1.814TyrThr: 1.814 ± 0.068
2.212TyrVal: 2.212 ± 0.076
0.417TyrTrp: 0.417 ± 0.035
1.376TyrTyr: 1.376 ± 0.07
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1263 proteins (369316 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski