Microbacterium phage Sharkboy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dismasvirus; unclassified Dismasvirus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A516KUD8|A0A516KUD8_9CAUD Uncharacterized protein OS=Microbacterium phage Sharkboy OX=2590938 GN=65 PE=4 SV=1
MM1 pKa = 7.47TGGAPEE7 pKa = 3.83PAGYY11 pKa = 10.73VLVLTAPDD19 pKa = 3.74DD20 pKa = 4.3HH21 pKa = 6.71PTEE24 pKa = 4.45PSQTFVAVRR33 pKa = 11.84APEE36 pKa = 4.02DD37 pKa = 3.3EE38 pKa = 4.08GRR40 pKa = 11.84LFEE43 pKa = 4.41TVEE46 pKa = 3.82EE47 pKa = 3.98LLAFVEE53 pKa = 4.54EE54 pKa = 4.14MSPFVEE60 pKa = 4.6GTLASYY66 pKa = 10.81LPVYY70 pKa = 10.18PSVAPAEE77 pKa = 4.26GLADD81 pKa = 3.99ALRR84 pKa = 11.84SALL87 pKa = 3.84

Molecular weight: 9.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A516KUE6|A0A516KUE6_9CAUD Uncharacterized protein OS=Microbacterium phage Sharkboy OX=2590938 GN=61 PE=4 SV=1
MM1 pKa = 7.41ARR3 pKa = 11.84ALVFTTDD10 pKa = 3.16TVALRR15 pKa = 11.84DD16 pKa = 3.7PAGGPVRR23 pKa = 11.84EE24 pKa = 4.3YY25 pKa = 11.27RR26 pKa = 11.84LGTPDD31 pKa = 3.31GEE33 pKa = 4.41GRR35 pKa = 11.84IILTPVDD42 pKa = 4.02QVPTPQQVEE51 pKa = 3.89AFRR54 pKa = 11.84RR55 pKa = 11.84AWHH58 pKa = 5.97TADD61 pKa = 3.8SLGLKK66 pKa = 9.67GDD68 pKa = 3.93RR69 pKa = 11.84VRR71 pKa = 11.84HH72 pKa = 5.17GLFAALNAWRR82 pKa = 11.84RR83 pKa = 11.84PP84 pKa = 3.59

Molecular weight: 9.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

13181

39

876

196.7

21.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.683 ± 0.328

0.425 ± 0.084

6.229 ± 0.267

6.191 ± 0.253

3.353 ± 0.156

8.838 ± 0.341

1.768 ± 0.151

3.763 ± 0.176

4.104 ± 0.247

8.785 ± 0.319

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.026 ± 0.121

2.891 ± 0.206

5.25 ± 0.201

3.566 ± 0.186

6.396 ± 0.327

5.576 ± 0.263

6.191 ± 0.327

8.489 ± 0.343

2.056 ± 0.151

2.42 ± 0.141
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski