Streptococcus phage phiST1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1IR90|M1IR90_9CAUD Uncharacterized protein OS=Streptococcus phage phiST1 OX=1277892 GN=ST1_005 PE=4 SV=1
MM1 pKa = 7.94RR2 pKa = 11.84IGVEE6 pKa = 3.87CDD8 pKa = 2.7SGVFVSNQLGYY19 pKa = 10.5GQYY22 pKa = 10.99LDD24 pKa = 5.38AVCQQVADD32 pKa = 3.47IGVAEE37 pKa = 4.53RR38 pKa = 11.84VDD40 pKa = 3.4MDD42 pKa = 3.95RR43 pKa = 11.84PANQFFQSLVV53 pKa = 3.31

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1HNV6|M1HNV6_9CAUD Phage minor structural protein OS=Streptococcus phage phiST1 OX=1277892 GN=ST1_0018 PE=4 SV=1
MM1 pKa = 7.73ADD3 pKa = 3.04VRR5 pKa = 11.84VTVDD9 pKa = 3.36LAGVEE14 pKa = 4.6KK15 pKa = 10.5KK16 pKa = 10.73VSPQSMQRR24 pKa = 11.84GKK26 pKa = 10.05IAAGSEE32 pKa = 3.87ALLIMDD38 pKa = 4.07SSVPLRR44 pKa = 11.84AGGGALRR51 pKa = 11.84ASGRR55 pKa = 11.84VEE57 pKa = 3.87PNGNASYY64 pKa = 8.81NTVYY68 pKa = 10.93ARR70 pKa = 11.84AQFHH74 pKa = 5.45GTNGIVVFRR83 pKa = 11.84KK84 pKa = 8.55YY85 pKa = 6.51TTAGTGKK92 pKa = 10.27RR93 pKa = 11.84WDD95 pKa = 4.08KK96 pKa = 10.35PLKK99 pKa = 10.87ANIEE103 pKa = 4.18RR104 pKa = 11.84LKK106 pKa = 10.67KK107 pKa = 10.31AAIKK111 pKa = 10.82GMGIRR116 pKa = 4.21

Molecular weight: 12.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12216

51

1480

218.1

24.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.818 ± 0.648

0.393 ± 0.061

6.745 ± 0.412

6.442 ± 0.484

3.741 ± 0.21

7.171 ± 0.56

1.482 ± 0.139

6.876 ± 0.27

7.867 ± 0.38

7.646 ± 0.43

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.263

5.452 ± 0.289

2.824 ± 0.182

4.036 ± 0.293

4.003 ± 0.265

6.197 ± 0.436

6.901 ± 0.491

6.614 ± 0.396

1.179 ± 0.172

4.085 ± 0.35
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski