Microbacterium phage Lovelyunicorn

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ilzatvirus; unclassified Ilzatvirus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N0A493|A0A6N0A493_9CAUD Minor tail protein OS=Microbacterium phage Lovelyunicorn OX=2744009 GN=20 PE=4 SV=1
MM1 pKa = 7.81ACTDD5 pKa = 4.43CDD7 pKa = 4.55DD8 pKa = 4.15KK9 pKa = 11.46QQEE12 pKa = 4.4PFSEE16 pKa = 4.6TEE18 pKa = 3.94GEE20 pKa = 4.5GTVDD24 pKa = 3.4TQEE27 pKa = 4.49TPSEE31 pKa = 4.49VYY33 pKa = 10.59DD34 pKa = 3.71HH35 pKa = 7.36AEE37 pKa = 3.76HH38 pKa = 6.96FGPPEE43 pKa = 3.55AA44 pKa = 5.69

Molecular weight: 4.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N0A7N4|A0A6N0A7N4_9CAUD DNA helicase OS=Microbacterium phage Lovelyunicorn OX=2744009 GN=41 PE=4 SV=1
MM1 pKa = 7.48SGQVLEE7 pKa = 4.92APVSARR13 pKa = 11.84FHH15 pKa = 7.35ASGQQDD21 pKa = 3.08RR22 pKa = 11.84RR23 pKa = 11.84TARR26 pKa = 11.84QDD28 pKa = 3.13GVVCVNRR35 pKa = 11.84LRR37 pKa = 11.84VRR39 pKa = 11.84ATVQNIDD46 pKa = 3.79GQWAEE51 pKa = 4.03VKK53 pKa = 10.57LSVKK57 pKa = 10.05IGEE60 pKa = 4.23GWFPLEE66 pKa = 4.2GGKK69 pKa = 10.12LRR71 pKa = 11.84VARR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84SGQRR80 pKa = 11.84EE81 pKa = 3.92AQEE84 pKa = 4.56LYY86 pKa = 10.49VVSALRR92 pKa = 11.84RR93 pKa = 11.84SLAAHH98 pKa = 6.11GVAVLSLDD106 pKa = 3.34GSEE109 pKa = 3.88QRR111 pKa = 11.84VGDD114 pKa = 3.59GVTDD118 pKa = 3.43YY119 pKa = 10.92RR120 pKa = 11.84YY121 pKa = 7.37WASMGSRR128 pKa = 11.84LTYY131 pKa = 10.53ALEE134 pKa = 3.91VSEE137 pKa = 4.91AGVVSVVNLPP147 pKa = 3.41

Molecular weight: 16.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13095

44

808

207.9

22.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.79 ± 0.423

0.649 ± 0.087

6.506 ± 0.32

6.522 ± 0.521

3.093 ± 0.204

7.82 ± 0.348

1.863 ± 0.166

5.094 ± 0.393

4.712 ± 0.35

8.538 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.512 ± 0.172

3.177 ± 0.172

4.903 ± 0.258

4.04 ± 0.206

5.727 ± 0.341

5.995 ± 0.285

6.445 ± 0.339

7.392 ± 0.312

2.138 ± 0.244

3.085 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski