Amanita thiersii Skay4041

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Amanitaceae; Amanita; Amanita thiersii

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10343 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A9NXE0|A0A2A9NXE0_9AGAR Uncharacterized protein OS=Amanita thiersii Skay4041 OX=703135 GN=AMATHDRAFT_84058 PE=3 SV=1
MM1 pKa = 7.6ASDD4 pKa = 4.39PLSLTTIARR13 pKa = 11.84NKK15 pKa = 7.45MHH17 pKa = 7.03SSFGSRR23 pKa = 11.84DD24 pKa = 3.27NCSLHH29 pKa = 6.69RR30 pKa = 11.84WVLLKK35 pKa = 11.03NSISVSQSPTLIGATINTLPDD56 pKa = 3.53ASSLCSAGGYY66 pKa = 10.57DD67 pKa = 5.34DD68 pKa = 6.21ADD70 pKa = 3.96DD71 pKa = 4.65RR72 pKa = 11.84NEE74 pKa = 4.44SGDD77 pKa = 3.42SDD79 pKa = 4.69SFFFPDD85 pKa = 3.0AGQIVSSSQTCLSEE99 pKa = 4.5AQWLDD104 pKa = 3.45TLLKK108 pKa = 10.57EE109 pKa = 4.83LGEE112 pKa = 5.14DD113 pKa = 3.6EE114 pKa = 5.56DD115 pKa = 5.6DD116 pKa = 4.96DD117 pKa = 4.66YY118 pKa = 12.07LVEE121 pKa = 5.03SDD123 pKa = 4.18VASPPAPVEE132 pKa = 4.94DD133 pKa = 4.99DD134 pKa = 5.71DD135 pKa = 5.34EE136 pKa = 4.72DD137 pKa = 5.56APLSPLVSPMASSDD151 pKa = 3.75DD152 pKa = 3.86LHH154 pKa = 6.56NQPQYY159 pKa = 11.14YY160 pKa = 9.76SPPRR164 pKa = 11.84QFPYY168 pKa = 10.61PRR170 pKa = 11.84FLHH173 pKa = 6.32PLLHH177 pKa = 6.83PFDD180 pKa = 5.42FDD182 pKa = 3.66QGMQSSISSLPPPYY196 pKa = 10.09DD197 pKa = 4.35DD198 pKa = 4.81PLPYY202 pKa = 10.53HH203 pKa = 6.76NIDD206 pKa = 3.5DD207 pKa = 4.19TQEE210 pKa = 3.93YY211 pKa = 10.01AVPDD215 pKa = 4.31TIIEE219 pKa = 4.87DD220 pKa = 3.62ISDD223 pKa = 4.41DD224 pKa = 4.02EE225 pKa = 5.67SDD227 pKa = 3.82TPSTPSYY234 pKa = 10.19GRR236 pKa = 11.84SSASLSLSDD245 pKa = 4.21IPSIPSPTEE254 pKa = 3.22RR255 pKa = 11.84SSRR258 pKa = 11.84TRR260 pKa = 11.84QATLQVYY267 pKa = 9.93DD268 pKa = 4.32DD269 pKa = 4.3KK270 pKa = 11.65DD271 pKa = 3.78VYY273 pKa = 10.61FYY275 pKa = 10.54PSEE278 pKa = 4.24SDD280 pKa = 3.61PLPFSNDD287 pKa = 2.47HH288 pKa = 6.24HH289 pKa = 7.51SSYY292 pKa = 10.95NAYY295 pKa = 9.74RR296 pKa = 11.84ACC298 pKa = 4.61

Molecular weight: 32.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A9NSE4|A0A2A9NSE4_9AGAR ACT_7 domain-containing protein OS=Amanita thiersii Skay4041 OX=703135 GN=AMATHDRAFT_140371 PE=4 SV=1
SS1 pKa = 6.08HH2 pKa = 7.4TNHH5 pKa = 5.76NQIKK9 pKa = 8.53KK10 pKa = 9.0AHH12 pKa = 6.46RR13 pKa = 11.84NGIKK17 pKa = 9.98KK18 pKa = 8.27PQRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84SLKK28 pKa = 9.88GVRR31 pKa = 11.84FRR33 pKa = 11.84RR34 pKa = 11.84NARR37 pKa = 11.84HH38 pKa = 6.26ALVGSNKK45 pKa = 9.47ARR47 pKa = 11.84SEE49 pKa = 4.14AKK51 pKa = 9.97KK52 pKa = 10.42AAAA55 pKa = 4.29

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10343

0

10343

4370377

49

5134

422.5

46.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.632 ± 0.021

1.247 ± 0.009

5.393 ± 0.017

5.784 ± 0.023

3.771 ± 0.016

6.404 ± 0.027

2.599 ± 0.012

5.306 ± 0.017

4.814 ± 0.026

9.312 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.158 ± 0.009

3.87 ± 0.013

6.203 ± 0.024

4.048 ± 0.017

5.951 ± 0.02

8.741 ± 0.03

6.252 ± 0.015

6.273 ± 0.017

1.416 ± 0.009

2.827 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski