Salmonella phage SFP10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; Cvivirinae; Kuttervirus; Salmonella virus SFP10

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4WAV1|G4WAV1_9CAUD Hypothetical phage protein OS=Salmonella phage SFP10 OX=1080800 GN=SFP_0184 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.23LNKK5 pKa = 9.49ILLVCALAFSTTACSTLLDD24 pKa = 3.75VASTVDD30 pKa = 3.92LDD32 pKa = 4.74APTFTNQQAVNKK44 pKa = 9.85MEE46 pKa = 4.2DD47 pKa = 3.51TIKK50 pKa = 11.04AHH52 pKa = 6.87AALDD56 pKa = 3.77NTTPGPLQTVCNYY69 pKa = 10.58DD70 pKa = 3.97DD71 pKa = 5.54SIQEE75 pKa = 4.24DD76 pKa = 3.93EE77 pKa = 4.95TYY79 pKa = 10.8HH80 pKa = 5.37CTTYY84 pKa = 10.81VKK86 pKa = 10.2EE87 pKa = 4.33SSVVLYY93 pKa = 10.72ADD95 pKa = 3.77CTEE98 pKa = 4.32EE99 pKa = 3.89QCTATGYY106 pKa = 10.78DD107 pKa = 3.34QVEE110 pKa = 4.46KK111 pKa = 10.86SDD113 pKa = 3.61EE114 pKa = 3.95

Molecular weight: 12.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4WAQ3|G4WAQ3_9CAUD Uncharacterized protein OS=Salmonella phage SFP10 OX=1080800 GN=SFP_0136 PE=4 SV=1
MM1 pKa = 7.37ACKK4 pKa = 9.41TPEE7 pKa = 3.85MKK9 pKa = 10.54LVVSSTGRR17 pKa = 11.84RR18 pKa = 11.84SRR20 pKa = 11.84SRR22 pKa = 11.84NLITRR27 pKa = 11.84LDD29 pKa = 3.63ILRR32 pKa = 11.84QKK34 pKa = 9.5YY35 pKa = 7.98QAHH38 pKa = 5.62SVFTRR43 pKa = 11.84IRR45 pKa = 11.84NALKK49 pKa = 10.19EE50 pKa = 3.98GRR52 pKa = 11.84TEE54 pKa = 4.12LEE56 pKa = 3.91LYY58 pKa = 10.03RR59 pKa = 11.84PNGSTRR65 pKa = 11.84AYY67 pKa = 8.21QTTDD71 pKa = 2.69GLLEE75 pKa = 4.85LIRR78 pKa = 11.84LSGMSIEE85 pKa = 4.55PRR87 pKa = 11.84SSGTPLCSLYY97 pKa = 10.99VISNLGALL105 pKa = 4.07

Molecular weight: 11.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

201

0

201

48148

38

1612

239.5

27.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.638 ± 0.161

1.122 ± 0.066

6.441 ± 0.113

6.569 ± 0.199

4.268 ± 0.085

6.711 ± 0.202

1.909 ± 0.101

6.38 ± 0.124

6.368 ± 0.179

8.025 ± 0.157

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.897 ± 0.089

5.261 ± 0.118

3.876 ± 0.105

3.813 ± 0.087

4.885 ± 0.121

6.322 ± 0.155

5.99 ± 0.204

7.244 ± 0.163

1.375 ± 0.063

3.907 ± 0.107
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski