Psathyrella aberdarensis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Psathyrellaceae; Candolleomyces

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14686 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q2D6S1|A0A4Q2D6S1_9AGAR GST N-terminal domain-containing protein OS=Psathyrella aberdarensis OX=2316362 GN=EST38_g10682 PE=4 SV=1
MM1 pKa = 7.51VKK3 pKa = 9.59TNPFNFVIAILFASFLHH20 pKa = 6.56VSAQTYY26 pKa = 7.66TVTNRR31 pKa = 11.84CPSAIEE37 pKa = 4.06LFIGQTSEE45 pKa = 4.69GNLATNQSIVKK56 pKa = 8.8TGLGTSAGFFYY67 pKa = 10.66TKK69 pKa = 10.95ANGGDD74 pKa = 3.95DD75 pKa = 4.75DD76 pKa = 6.8DD77 pKa = 6.98GDD79 pKa = 4.34LEE81 pKa = 3.98AARR84 pKa = 11.84AGFYY88 pKa = 10.54FEE90 pKa = 4.49PDD92 pKa = 2.5YY93 pKa = 10.51WYY95 pKa = 10.98YY96 pKa = 11.22YY97 pKa = 9.9IVRR100 pKa = 11.84DD101 pKa = 5.22DD102 pKa = 4.18GDD104 pKa = 4.6LNTGISITPDD114 pKa = 2.97QPADD118 pKa = 3.29NGPPVFHH125 pKa = 6.93GPVPPPPDD133 pKa = 3.78APAPSPPLYY142 pKa = 9.63QCRR145 pKa = 11.84VPGTNFEE152 pKa = 4.25ITFCPSGVWPTDD164 pKa = 3.8DD165 pKa = 6.37DD166 pKa = 5.56NDD168 pKa = 5.34DD169 pKa = 4.05NDD171 pKa = 4.77DD172 pKa = 4.88DD173 pKa = 5.51RR174 pKa = 11.84DD175 pKa = 3.62

Molecular weight: 19.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q2DPA6|A0A4Q2DPA6_9AGAR PX domain-containing protein OS=Psathyrella aberdarensis OX=2316362 GN=EST38_g5186 PE=3 SV=1
MM1 pKa = 7.3SGSGGHH7 pKa = 6.02HH8 pKa = 5.7TRR10 pKa = 11.84QGSSTAVKK18 pKa = 10.41VGLHH22 pKa = 4.98GHH24 pKa = 5.79SLNPTPYY31 pKa = 9.01HH32 pKa = 5.78HH33 pKa = 6.97HH34 pKa = 7.14HH35 pKa = 5.93NTGNVTSRR43 pKa = 11.84EE44 pKa = 3.94VVEE47 pKa = 5.22AGNSDD52 pKa = 3.56PRR54 pKa = 11.84SDD56 pKa = 3.5GLAGRR61 pKa = 11.84TPSPTPSEE69 pKa = 3.95LRR71 pKa = 11.84EE72 pKa = 4.2LKK74 pKa = 10.2PAANGWGTSRR84 pKa = 11.84EE85 pKa = 4.07RR86 pKa = 11.84LWFFAAIIILIALIALAWIYY106 pKa = 10.74DD107 pKa = 3.74KK108 pKa = 11.41QIVRR112 pKa = 11.84TLAPAATWLHH122 pKa = 5.84DD123 pKa = 3.76TPGAFAIPMAVLIVISFPPLFGHH146 pKa = 6.77AVVAILCGFVWGLGIGFGIVAGGTFLGEE174 pKa = 3.7IGHH177 pKa = 6.75FYY179 pKa = 10.87ASKK182 pKa = 10.56LVFRR186 pKa = 11.84TRR188 pKa = 11.84GEE190 pKa = 3.85KK191 pKa = 10.55LEE193 pKa = 4.08RR194 pKa = 11.84TNISHH199 pKa = 7.15ACLAQVVRR207 pKa = 11.84DD208 pKa = 3.59GGIRR212 pKa = 11.84ITLIARR218 pKa = 11.84LSAIPAQTTTTVFSTCGVGIIIFAISTLLSMPKK251 pKa = 9.58QALGVYY257 pKa = 10.1LGVILLRR264 pKa = 11.84SRR266 pKa = 11.84FEE268 pKa = 4.23TRR270 pKa = 11.84SSTEE274 pKa = 3.85RR275 pKa = 11.84IVTSVVLAVTMLITAVATWYY295 pKa = 9.9IGRR298 pKa = 11.84QMDD301 pKa = 3.44RR302 pKa = 11.84TKK304 pKa = 10.7PQVIYY309 pKa = 10.47KK310 pKa = 9.87RR311 pKa = 11.84RR312 pKa = 11.84KK313 pKa = 8.31ARR315 pKa = 11.84QARR318 pKa = 11.84LTQASSPTHH327 pKa = 6.81PYY329 pKa = 11.14LMAASGNGQSTMGSEE344 pKa = 4.32STVMFNPSNDD354 pKa = 3.19NLRR357 pKa = 11.84DD358 pKa = 3.63QNSRR362 pKa = 11.84QAGSSQLALLPPVEE376 pKa = 4.62APQPRR381 pKa = 11.84RR382 pKa = 11.84PFPP385 pKa = 4.1

Molecular weight: 41.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14686

0

14686

6965245

10

4979

474.3

52.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.337 ± 0.014

1.164 ± 0.006

5.792 ± 0.012

6.381 ± 0.018

3.825 ± 0.011

6.422 ± 0.018

2.479 ± 0.009

4.922 ± 0.013

4.981 ± 0.019

9.38 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.914 ± 0.007

3.632 ± 0.01

6.387 ± 0.02

3.879 ± 0.013

5.972 ± 0.014

8.394 ± 0.023

5.862 ± 0.014

6.186 ± 0.013

1.394 ± 0.006

2.654 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski