Dragonfly orbiculatusvirus

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0A1L1|K0A1L1_9VIRU Replication-associated protein OS=Dragonfly orbiculatusvirus OX=1234874 PE=4 SV=1
MM1 pKa = 7.13YY2 pKa = 10.38KK3 pKa = 9.99RR4 pKa = 11.84WCFTLNNYY12 pKa = 10.08SVVCNWEE19 pKa = 3.79EE20 pKa = 3.93FFNNGCSTGNKK31 pKa = 7.92LCRR34 pKa = 11.84YY35 pKa = 7.82VVGYY39 pKa = 9.82EE40 pKa = 3.57IGEE43 pKa = 4.23SGTRR47 pKa = 11.84HH48 pKa = 4.69LQGYY52 pKa = 8.64CVLSRR57 pKa = 11.84SQRR60 pKa = 11.84LSYY63 pKa = 8.74VTKK66 pKa = 10.76LFDD69 pKa = 3.64GAHH72 pKa = 5.92WEE74 pKa = 4.26GARR77 pKa = 11.84GSDD80 pKa = 3.33QQNYY84 pKa = 10.15DD85 pKa = 3.38YY86 pKa = 9.35CTKK89 pKa = 10.42SGSFFRR95 pKa = 11.84GGDD98 pKa = 3.3WNVLSRR104 pKa = 11.84SSSGSSDD111 pKa = 3.82RR112 pKa = 11.84IRR114 pKa = 11.84DD115 pKa = 3.81VIKK118 pKa = 9.61TLYY121 pKa = 10.67SMPGSEE127 pKa = 4.62VRR129 pKa = 11.84CSSTYY134 pKa = 10.07VRR136 pKa = 11.84SKK138 pKa = 10.38RR139 pKa = 11.84AIDD142 pKa = 3.68EE143 pKa = 4.27VVSEE147 pKa = 3.94YY148 pKa = 11.07RR149 pKa = 11.84EE150 pKa = 3.89FEE152 pKa = 4.03VRR154 pKa = 11.84KK155 pKa = 9.95LRR157 pKa = 11.84FRR159 pKa = 11.84EE160 pKa = 4.16LSTCLLKK167 pKa = 10.56LWQMSVLLKK176 pKa = 10.79LFNQNDD182 pKa = 3.97RR183 pKa = 11.84QVLWVVDD190 pKa = 3.73VAGGCGKK197 pKa = 8.41TFLSNILFSCYY208 pKa = 10.55SFDD211 pKa = 4.69LFDD214 pKa = 5.2GVTQAKK220 pKa = 9.57DD221 pKa = 2.94ICFLLSEE228 pKa = 4.79TINGIVFDD236 pKa = 3.94VTRR239 pKa = 11.84SDD241 pKa = 3.46SSQFSYY247 pKa = 9.31QTLEE251 pKa = 3.57ACKK254 pKa = 10.28NGFVMTGKK262 pKa = 10.77YY263 pKa = 7.95MGKK266 pKa = 9.17RR267 pKa = 11.84RR268 pKa = 11.84VFKK271 pKa = 10.41SCPVVIFSNCEE282 pKa = 3.4PDD284 pKa = 4.05LVRR287 pKa = 11.84LSSDD291 pKa = 2.3RR292 pKa = 11.84WVVYY296 pKa = 9.16NVPAQARR303 pKa = 11.84SQEE306 pKa = 4.52AIYY309 pKa = 10.32SPQALWPFKK318 pKa = 10.41EE319 pKa = 4.02IEE321 pKa = 4.13AVLLSEE327 pKa = 5.12IEE329 pKa = 4.0

Molecular weight: 37.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0A1L1|K0A1L1_9VIRU Replication-associated protein OS=Dragonfly orbiculatusvirus OX=1234874 PE=4 SV=1
MM1 pKa = 7.35SLRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84GRR8 pKa = 11.84KK9 pKa = 8.51RR10 pKa = 11.84RR11 pKa = 11.84YY12 pKa = 7.99IRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84YY18 pKa = 8.99GRR20 pKa = 11.84SRR22 pKa = 11.84RR23 pKa = 11.84SKK25 pKa = 9.29RR26 pKa = 11.84SCFPRR31 pKa = 11.84LNNFYY36 pKa = 11.17SKK38 pKa = 9.9MCIKK42 pKa = 10.38EE43 pKa = 4.07VTQFTATSNATHH55 pKa = 5.53VHH57 pKa = 6.32SISLANVNSYY67 pKa = 10.37VLQFLQNFKK76 pKa = 10.27YY77 pKa = 10.14YY78 pKa = 10.76RR79 pKa = 11.84MYY81 pKa = 11.44AMSVLLMPRR90 pKa = 11.84MNAIGATQSYY100 pKa = 7.89YY101 pKa = 10.61QPQNDD106 pKa = 3.06AHH108 pKa = 7.15LINTAPNLWTLFEE121 pKa = 4.84PEE123 pKa = 4.05TGEE126 pKa = 4.2VNDD129 pKa = 3.72VVALHH134 pKa = 5.89LHH136 pKa = 5.57PRR138 pKa = 11.84SRR140 pKa = 11.84LHH142 pKa = 6.62SFNKK146 pKa = 8.01TVRR149 pKa = 11.84RR150 pKa = 11.84FIKK153 pKa = 9.69LQPSLRR159 pKa = 11.84VNTADD164 pKa = 3.99LSNVDD169 pKa = 2.75IHH171 pKa = 7.69GGVFRR176 pKa = 11.84KK177 pKa = 7.89TWVAMADD184 pKa = 4.3FKK186 pKa = 11.63ASFGKK191 pKa = 10.11FVMAFSEE198 pKa = 4.44DD199 pKa = 3.45TKK201 pKa = 11.13SDD203 pKa = 3.71QNIHH207 pKa = 5.26TEE209 pKa = 3.8YY210 pKa = 11.22DD211 pKa = 3.69VVFKK215 pKa = 10.39YY216 pKa = 11.19YY217 pKa = 10.43IMLKK221 pKa = 10.54GSAPNLWSGEE231 pKa = 4.15VVTQQLPAPEE241 pKa = 4.38TVPLASSKK249 pKa = 10.93GGSVKK254 pKa = 10.38KK255 pKa = 10.54KK256 pKa = 10.44SGDD259 pKa = 3.18IPVIKK264 pKa = 10.16VRR266 pKa = 11.84QVDD269 pKa = 3.79TGPVPDD275 pKa = 4.0SHH277 pKa = 7.74GLGQKK282 pKa = 9.92SSAIEE287 pKa = 3.91KK288 pKa = 10.21VLEE291 pKa = 4.15EE292 pKa = 4.18EE293 pKa = 4.26MGLL296 pKa = 3.87

Molecular weight: 33.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

625

296

329

312.5

35.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.64 ± 1.016

2.56 ± 1.328

4.32 ± 0.426

4.96 ± 0.639

5.44 ± 0.501

6.08 ± 0.475

1.76 ± 0.903

3.84 ± 0.151

5.76 ± 0.703

8.16 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.69

4.96 ± 0.314

3.2 ± 1.078

4.0 ± 0.038

7.68 ± 0.302

10.4 ± 0.901

4.8 ± 0.427

8.8 ± 0.25

1.76 ± 0.526

4.48 ± 0.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski