Nodamura virus (strain Mag115) (NoV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Magsaviricetes; Nodamuvirales; Nodaviridae; Alphanodavirus; Nodamura virus

Average proteome isoelectric point is 8.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9IMM4|RDRP_NODAM RNA-directed RNA polymerase OS=Nodamura virus (strain Mag115) OX=914672 PE=1 SV=1
MM1 pKa = 7.54TNMSCAYY8 pKa = 9.37EE9 pKa = 4.38LIKK12 pKa = 10.72SLPAKK17 pKa = 10.39LEE19 pKa = 3.7QLAQEE24 pKa = 4.32TQATIQTLMIADD36 pKa = 3.9PNVNKK41 pKa = 10.33DD42 pKa = 3.23LRR44 pKa = 11.84AFCEE48 pKa = 4.09FLTVQHH54 pKa = 5.36QRR56 pKa = 11.84AYY58 pKa = 10.68RR59 pKa = 11.84ATNSLLIKK67 pKa = 9.98PRR69 pKa = 11.84VAAALRR75 pKa = 11.84GEE77 pKa = 4.02EE78 pKa = 4.39LDD80 pKa = 4.55LGEE83 pKa = 5.56ADD85 pKa = 3.02VAARR89 pKa = 11.84VRR91 pKa = 11.84QLKK94 pKa = 8.98QQLAEE99 pKa = 4.3LEE101 pKa = 4.33MEE103 pKa = 4.81IKK105 pKa = 10.23PGHH108 pKa = 5.04QQVAQVSGRR117 pKa = 11.84RR118 pKa = 11.84KK119 pKa = 9.06AAAAAPVAQLGRR131 pKa = 11.84VGVVNEE137 pKa = 4.01

Molecular weight: 15.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9IMM4-2|RDRP-2_NODAM Isoform of Q9IMM4 Isoform B1 of RNA-directed RNA polymerase OS=Nodamura virus (strain Mag115) OX=914672 PE=4 SV=1
MM1 pKa = 8.1RR2 pKa = 11.84LRR4 pKa = 11.84ANQVTSSQAGAAGSGDD20 pKa = 3.52ASNDD24 pKa = 3.24PNAHH28 pKa = 6.49DD29 pKa = 4.95RR30 pKa = 11.84GSQRR34 pKa = 11.84QQGSASVLRR43 pKa = 11.84VPDD46 pKa = 3.62RR47 pKa = 11.84AAPAGVSSDD56 pKa = 3.82EE57 pKa = 4.28QPAHH61 pKa = 4.72QTASRR66 pKa = 11.84SSASRR71 pKa = 11.84GGAGPGRR78 pKa = 11.84GGRR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84PGPPAKK89 pKa = 8.28TTAGGARR96 pKa = 11.84DD97 pKa = 3.94GNQARR102 pKa = 11.84APTSGPSKK110 pKa = 9.89RR111 pKa = 11.84QAEE114 pKa = 4.39GRR116 pKa = 11.84SRR118 pKa = 11.84SSRR121 pKa = 11.84GPAGSRR127 pKa = 11.84GRR129 pKa = 11.84GKK131 pKa = 10.6

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

2

5

1844

131

1043

368.8

40.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.093 ± 1.47

1.247 ± 0.213

5.206 ± 1.091

4.61 ± 0.688

3.2 ± 0.69

7.43 ± 1.072

1.898 ± 0.578

4.121 ± 0.587

3.579 ± 0.406

7.213 ± 0.922

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.061 ± 0.224

3.579 ± 0.762

5.857 ± 0.517

4.935 ± 1.2

8.785 ± 1.156

7.375 ± 0.984

5.64 ± 0.595

7.213 ± 0.613

1.139 ± 0.441

2.82 ± 0.555
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski