Amino acid dipepetide frequency for methanogenic archaeon ISO4-H5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.447AlaAla: 7.447 ± 0.203	1.324AlaCys: 1.324 ± 0.047	5.352AlaAsp: 5.352 ± 0.101	5.869AlaGlu: 5.869 ± 0.131	3.393AlaPhe: 3.393 ± 0.11	6.292AlaGly: 6.292 ± 0.128	1.258AlaHis: 1.258 ± 0.052	5.49AlaIle: 5.49 ± 0.123	4.743AlaLys: 4.743 ± 0.128	7.029AlaLeu: 7.029 ± 0.126	2.64AlaMet: 2.64 ± 0.083	2.683AlaAsn: 2.683 ± 0.074	2.486AlaPro: 2.486 ± 0.073	1.862AlaGln: 1.862 ± 0.07	3.419AlaArg: 3.419 ± 0.109	4.346AlaSer: 4.346 ± 0.108	3.915AlaThr: 3.915 ± 0.124	7.073AlaVal: 7.073 ± 0.135	0.6AlaTrp: 0.6 ± 0.038	2.782AlaTyr: 2.782 ± 0.072	0.0AlaXaa: 0.0 ± 0.0
Cys	1.214CysAla: 1.214 ± 0.046	0.365CysCys: 0.365 ± 0.028	1.025CysAsp: 1.025 ± 0.043	0.994CysGlu: 0.994 ± 0.04	0.567CysPhe: 0.567 ± 0.034	1.834CysGly: 1.834 ± 0.072	0.306CysHis: 0.306 ± 0.025	1.093CysIle: 1.093 ± 0.043	0.816CysLys: 0.816 ± 0.039	1.227CysLeu: 1.227 ± 0.052	0.466CysMet: 0.466 ± 0.027	0.555CysAsn: 0.555 ± 0.03	0.99CysPro: 0.99 ± 0.059	0.386CysGln: 0.386 ± 0.028	0.94CysArg: 0.94 ± 0.045	1.096CysSer: 1.096 ± 0.051	1.009CysThr: 1.009 ± 0.05	1.044CysVal: 1.044 ± 0.04	0.181CysTrp: 0.181 ± 0.018	0.56CysTyr: 0.56 ± 0.034	0.0CysXaa: 0.0 ± 0.0
Asp	5.288AspAla: 5.288 ± 0.095	0.98AspCys: 0.98 ± 0.039	3.57AspAsp: 3.57 ± 0.09	4.425AspGlu: 4.425 ± 0.1	2.514AspPhe: 2.514 ± 0.07	5.375AspGly: 5.375 ± 0.146	1.035AspHis: 1.035 ± 0.045	4.851AspIle: 4.851 ± 0.097	3.323AspLys: 3.323 ± 0.084	5.9AspLeu: 5.9 ± 0.112	2.147AspMet: 2.147 ± 0.064	2.171AspAsn: 2.171 ± 0.067	2.979AspPro: 2.979 ± 0.079	1.288AspGln: 1.288 ± 0.05	3.638AspArg: 3.638 ± 0.107	4.252AspSer: 4.252 ± 0.103	3.365AspThr: 3.365 ± 0.076	4.672AspVal: 4.672 ± 0.094	0.635AspTrp: 0.635 ± 0.04	2.674AspTyr: 2.674 ± 0.082	0.0AspXaa: 0.0 ± 0.0
Glu	5.357GluAla: 5.357 ± 0.112	0.997GluCys: 0.997 ± 0.042	4.171GluAsp: 4.171 ± 0.084	5.263GluGlu: 5.263 ± 0.158	2.64GluPhe: 2.64 ± 0.063	4.597GluGly: 4.597 ± 0.095	1.275GluHis: 1.275 ± 0.049	4.856GluIle: 4.856 ± 0.096	4.675GluLys: 4.675 ± 0.102	5.629GluLeu: 5.629 ± 0.118	2.304GluMet: 2.304 ± 0.07	2.906GluAsn: 2.906 ± 0.076	2.17GluPro: 2.17 ± 0.062	1.736GluGln: 1.736 ± 0.057	3.304GluArg: 3.304 ± 0.091	3.878GluSer: 3.878 ± 0.09	3.537GluThr: 3.537 ± 0.086	4.326GluVal: 4.326 ± 0.085	0.698GluTrp: 0.698 ± 0.033	2.655GluTyr: 2.655 ± 0.068	0.0GluXaa: 0.0 ± 0.0
Phe	2.751PheAla: 2.751 ± 0.085	0.719PheCys: 0.719 ± 0.032	2.958PheAsp: 2.958 ± 0.077	2.478PheGlu: 2.478 ± 0.059	1.467PhePhe: 1.467 ± 0.059	3.25PheGly: 3.25 ± 0.081	0.607PheHis: 0.607 ± 0.034	2.528PheIle: 2.528 ± 0.078	1.902PheLys: 1.902 ± 0.066	3.074PheLeu: 3.074 ± 0.089	1.261PheMet: 1.261 ± 0.056	1.481PheAsn: 1.481 ± 0.055	1.462PhePro: 1.462 ± 0.055	0.842PheGln: 0.842 ± 0.035	1.985PheArg: 1.985 ± 0.063	2.873PheSer: 2.873 ± 0.079	2.526PheThr: 2.526 ± 0.08	2.941PheVal: 2.941 ± 0.076	0.381PheTrp: 0.381 ± 0.029	1.413PheTyr: 1.413 ± 0.062	0.0PheXaa: 0.0 ± 0.0
Gly	5.549GlyAla: 5.549 ± 0.126	1.535GlyCys: 1.535 ± 0.059	4.444GlyAsp: 4.444 ± 0.107	4.32GlyGlu: 4.32 ± 0.089	3.095GlyPhe: 3.095 ± 0.08	5.366GlyGly: 5.366 ± 0.152	1.469GlyHis: 1.469 ± 0.054	5.836GlyIle: 5.836 ± 0.11	5.773GlyLys: 5.773 ± 0.11	6.154GlyLeu: 6.154 ± 0.126	2.653GlyMet: 2.653 ± 0.079	3.275GlyAsn: 3.275 ± 0.094	2.185GlyPro: 2.185 ± 0.066	1.698GlyGln: 1.698 ± 0.063	3.784GlyArg: 3.784 ± 0.1	5.239GlySer: 5.239 ± 0.148	5.443GlyThr: 5.443 ± 0.17	5.31GlyVal: 5.31 ± 0.105	0.947GlyTrp: 0.947 ± 0.058	3.158GlyTyr: 3.158 ± 0.101	0.0GlyXaa: 0.0 ± 0.0
His	1.152HisAla: 1.152 ± 0.047	0.343HisCys: 0.343 ± 0.024	1.03HisAsp: 1.03 ± 0.045	0.901HisGlu: 0.901 ± 0.04	0.712HisPhe: 0.712 ± 0.037	1.404HisGly: 1.404 ± 0.051	0.358HisHis: 0.358 ± 0.026	1.246HisIle: 1.246 ± 0.053	0.776HisLys: 0.776 ± 0.039	1.514HisLeu: 1.514 ± 0.057	0.586HisMet: 0.586 ± 0.032	0.63HisAsn: 0.63 ± 0.033	0.983HisPro: 0.983 ± 0.038	0.365HisGln: 0.365 ± 0.027	0.926HisArg: 0.926 ± 0.05	1.044HisSer: 1.044 ± 0.045	0.919HisThr: 0.919 ± 0.04	1.218HisVal: 1.218 ± 0.046	0.181HisTrp: 0.181 ± 0.017	0.607HisTyr: 0.607 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.782IleAla: 5.782 ± 0.121	1.197IleCys: 1.197 ± 0.046	4.627IleAsp: 4.627 ± 0.091	4.186IleGlu: 4.186 ± 0.096	2.257IlePhe: 2.257 ± 0.072	5.7IleGly: 5.7 ± 0.121	1.216IleHis: 1.216 ± 0.049	4.67IleIle: 4.67 ± 0.132	3.24IleLys: 3.24 ± 0.082	6.038IleLeu: 6.038 ± 0.142	1.879IleMet: 1.879 ± 0.052	2.439IleAsn: 2.439 ± 0.071	3.433IlePro: 3.433 ± 0.092	1.524IleGln: 1.524 ± 0.051	3.661IleArg: 3.661 ± 0.092	4.894IleSer: 4.894 ± 0.109	4.279IleThr: 4.279 ± 0.114	5.298IleVal: 5.298 ± 0.112	0.56IleTrp: 0.56 ± 0.037	2.077IleTyr: 2.077 ± 0.065	0.0IleXaa: 0.0 ± 0.0
Lys	5.227LysAla: 5.227 ± 0.118	0.884LysCys: 0.884 ± 0.049	4.219LysAsp: 4.219 ± 0.1	4.606LysGlu: 4.606 ± 0.106	1.783LysPhe: 1.783 ± 0.059	4.179LysGly: 4.179 ± 0.087	0.933LysHis: 0.933 ± 0.039	4.245LysIle: 4.245 ± 0.096	4.454LysLys: 4.454 ± 0.122	4.576LysLeu: 4.576 ± 0.1	1.738LysMet: 1.738 ± 0.062	2.547LysAsn: 2.547 ± 0.068	1.879LysPro: 1.879 ± 0.064	1.542LysGln: 1.542 ± 0.054	2.669LysArg: 2.669 ± 0.087	3.299LysSer: 3.299 ± 0.077	3.555LysThr: 3.555 ± 0.085	4.275LysVal: 4.275 ± 0.099	0.635LysTrp: 0.635 ± 0.038	2.234LysTyr: 2.234 ± 0.064	0.0LysXaa: 0.0 ± 0.0
Leu	6.641LeuAla: 6.641 ± 0.163	1.411LeuCys: 1.411 ± 0.052	5.397LeuAsp: 5.397 ± 0.112	5.321LeuGlu: 5.321 ± 0.103	3.292LeuPhe: 3.292 ± 0.105	6.447LeuGly: 6.447 ± 0.104	1.251LeuHis: 1.251 ± 0.046	5.46LeuIle: 5.46 ± 0.122	5.121LeuLys: 5.121 ± 0.104	6.6LeuLeu: 6.6 ± 0.162	2.827LeuMet: 2.827 ± 0.086	3.306LeuAsn: 3.306 ± 0.079	3.346LeuPro: 3.346 ± 0.075	1.905LeuGln: 1.905 ± 0.057	4.204LeuArg: 4.204 ± 0.102	5.951LeuSer: 5.951 ± 0.116	4.945LeuThr: 4.945 ± 0.097	5.82LeuVal: 5.82 ± 0.128	0.663LeuTrp: 0.663 ± 0.038	2.739LeuTyr: 2.739 ± 0.079	0.0LeuXaa: 0.0 ± 0.0
Met	2.867MetAla: 2.867 ± 0.076	0.466MetCys: 0.466 ± 0.03	2.267MetAsp: 2.267 ± 0.077	2.112MetGlu: 2.112 ± 0.062	1.134MetPhe: 1.134 ± 0.045	2.37MetGly: 2.37 ± 0.07	0.532MetHis: 0.532 ± 0.031	2.001MetIle: 2.001 ± 0.067	2.058MetLys: 2.058 ± 0.069	2.417MetLeu: 2.417 ± 0.079	1.164MetMet: 1.164 ± 0.051	1.279MetAsn: 1.279 ± 0.051	1.199MetPro: 1.199 ± 0.047	0.786MetGln: 0.786 ± 0.041	1.528MetArg: 1.528 ± 0.057	2.297MetSer: 2.297 ± 0.069	1.924MetThr: 1.924 ± 0.057	2.271MetVal: 2.271 ± 0.074	0.24MetTrp: 0.24 ± 0.02	1.091MetTyr: 1.091 ± 0.049	0.0MetXaa: 0.0 ± 0.0
Asn	3.048AsnAla: 3.048 ± 0.095	0.668AsnCys: 0.668 ± 0.037	2.257AsnAsp: 2.257 ± 0.061	2.22AsnGlu: 2.22 ± 0.061	1.154AsnPhe: 1.154 ± 0.05	3.753AsnGly: 3.753 ± 0.105	0.731AsnHis: 0.731 ± 0.038	2.841AsnIle: 2.841 ± 0.076	1.895AsnLys: 1.895 ± 0.064	3.283AsnLeu: 3.283 ± 0.079	1.169AsnMet: 1.169 ± 0.044	1.559AsnAsn: 1.559 ± 0.061	2.008AsnPro: 2.008 ± 0.058	0.84AsnGln: 0.84 ± 0.049	1.99AsnArg: 1.99 ± 0.056	2.281AsnSer: 2.281 ± 0.078	2.177AsnThr: 2.177 ± 0.077	2.918AsnVal: 2.918 ± 0.082	0.355AsnTrp: 0.355 ± 0.026	1.456AsnTyr: 1.456 ± 0.06	0.0AsnXaa: 0.0 ± 0.0
Pro	3.198ProAla: 3.198 ± 0.084	0.51ProCys: 0.51 ± 0.032	2.902ProAsp: 2.902 ± 0.077	3.616ProGlu: 3.616 ± 0.102	1.58ProPhe: 1.58 ± 0.062	2.897ProGly: 2.897 ± 0.068	0.699ProHis: 0.699 ± 0.04	2.253ProIle: 2.253 ± 0.067	2.144ProLys: 2.144 ± 0.07	3.007ProLeu: 3.007 ± 0.079	1.152ProMet: 1.152 ± 0.043	1.373ProAsn: 1.373 ± 0.046	1.202ProPro: 1.202 ± 0.057	0.997ProGln: 0.997 ± 0.046	1.67ProArg: 1.67 ± 0.065	2.499ProSer: 2.499 ± 0.077	1.982ProThr: 1.982 ± 0.067	3.374ProVal: 3.374 ± 0.073	0.338ProTrp: 0.338 ± 0.026	1.515ProTyr: 1.515 ± 0.054	0.0ProXaa: 0.0 ± 0.0
Gln	1.937GlnAla: 1.937 ± 0.06	0.374GlnCys: 0.374 ± 0.025	1.314GlnAsp: 1.314 ± 0.045	1.542GlnGlu: 1.542 ± 0.053	0.931GlnPhe: 0.931 ± 0.041	1.528GlnGly: 1.528 ± 0.057	0.371GlnHis: 0.371 ± 0.023	1.677GlnIle: 1.677 ± 0.05	1.648GlnLys: 1.648 ± 0.054	1.822GlnLeu: 1.822 ± 0.05	1.047GlnMet: 1.047 ± 0.04	1.002GlnAsn: 1.002 ± 0.042	0.773GlnPro: 0.773 ± 0.035	0.628GlnGln: 0.628 ± 0.037	1.234GlnArg: 1.234 ± 0.049	1.425GlnSer: 1.425 ± 0.055	1.296GlnThr: 1.296 ± 0.053	1.521GlnVal: 1.521 ± 0.05	0.28GlnTrp: 0.28 ± 0.025	1.108GlnTyr: 1.108 ± 0.051	0.0GlnXaa: 0.0 ± 0.0
Arg	3.353ArgAla: 3.353 ± 0.075	0.865ArgCys: 0.865 ± 0.049	3.186ArgAsp: 3.186 ± 0.113	3.496ArgGlu: 3.496 ± 0.099	2.262ArgPhe: 2.262 ± 0.073	3.036ArgGly: 3.036 ± 0.077	0.837ArgHis: 0.837 ± 0.034	3.715ArgIle: 3.715 ± 0.099	3.476ArgLys: 3.476 ± 0.095	3.932ArgLeu: 3.932 ± 0.107	1.877ArgMet: 1.877 ± 0.067	2.253ArgAsn: 2.253 ± 0.064	1.783ArgPro: 1.783 ± 0.072	1.265ArgGln: 1.265 ± 0.045	2.878ArgArg: 2.878 ± 0.108	3.04ArgSer: 3.04 ± 0.089	2.662ArgThr: 2.662 ± 0.067	2.96ArgVal: 2.96 ± 0.082	0.473ArgTrp: 0.473 ± 0.03	2.109ArgTyr: 2.109 ± 0.064	0.0ArgXaa: 0.0 ± 0.0
Ser	5.178SerAla: 5.178 ± 0.113	0.927SerCys: 0.927 ± 0.047	4.472SerAsp: 4.472 ± 0.096	4.538SerGlu: 4.538 ± 0.1	2.702SerPhe: 2.702 ± 0.084	5.55SerGly: 5.55 ± 0.149	1.018SerHis: 1.018 ± 0.045	4.346SerIle: 4.346 ± 0.109	3.659SerLys: 3.659 ± 0.095	5.274SerLeu: 5.274 ± 0.112	1.971SerMet: 1.971 ± 0.063	2.33SerAsn: 2.33 ± 0.067	2.215SerPro: 2.215 ± 0.063	1.609SerGln: 1.609 ± 0.053	3.003SerArg: 3.003 ± 0.085	4.223SerSer: 4.223 ± 0.123	3.33SerThr: 3.33 ± 0.093	5.448SerVal: 5.448 ± 0.133	0.68SerTrp: 0.68 ± 0.039	2.245SerTyr: 2.245 ± 0.073	0.0SerXaa: 0.0 ± 0.0
Thr	4.736ThrAla: 4.736 ± 0.121	0.846ThrCys: 0.846 ± 0.045	3.887ThrAsp: 3.887 ± 0.095	3.885ThrGlu: 3.885 ± 0.094	2.453ThrPhe: 2.453 ± 0.086	4.926ThrGly: 4.926 ± 0.113	0.931ThrHis: 0.931 ± 0.04	3.931ThrIle: 3.931 ± 0.134	2.726ThrLys: 2.726 ± 0.077	4.82ThrLeu: 4.82 ± 0.122	1.528ThrMet: 1.528 ± 0.057	1.923ThrAsn: 1.923 ± 0.059	2.603ThrPro: 2.603 ± 0.071	1.308ThrGln: 1.308 ± 0.055	2.121ThrArg: 2.121 ± 0.06	3.449ThrSer: 3.449 ± 0.106	3.132ThrThr: 3.132 ± 0.107	6.034ThrVal: 6.034 ± 0.188	0.498ThrTrp: 0.498 ± 0.03	2.372ThrTyr: 2.372 ± 0.124	0.0ThrXaa: 0.0 ± 0.0
Val	5.693ValAla: 5.693 ± 0.117	1.333ValCys: 1.333 ± 0.047	4.696ValAsp: 4.696 ± 0.106	4.426ValGlu: 4.426 ± 0.106	3.221ValPhe: 3.221 ± 0.089	5.037ValGly: 5.037 ± 0.115	1.22ValHis: 1.22 ± 0.045	5.155ValIle: 5.155 ± 0.097	4.667ValLys: 4.667 ± 0.085	6.372ValLeu: 6.372 ± 0.129	2.271ValMet: 2.271 ± 0.079	2.756ValAsn: 2.756 ± 0.08	3.53ValPro: 3.53 ± 0.076	1.662ValGln: 1.662 ± 0.05	3.931ValArg: 3.931 ± 0.092	5.305ValSer: 5.305 ± 0.118	5.013ValThr: 5.013 ± 0.161	5.103ValVal: 5.103 ± 0.114	0.642ValTrp: 0.642 ± 0.035	2.758ValTyr: 2.758 ± 0.1	0.0ValXaa: 0.0 ± 0.0
Trp	0.71TrpAla: 0.71 ± 0.042	0.165TrpCys: 0.165 ± 0.016	0.644TrpAsp: 0.644 ± 0.038	0.614TrpGlu: 0.614 ± 0.033	0.437TrpPhe: 0.437 ± 0.031	0.585TrpGly: 0.585 ± 0.036	0.167TrpHis: 0.167 ± 0.018	0.647TrpIle: 0.647 ± 0.031	0.654TrpLys: 0.654 ± 0.035	0.712TrpLeu: 0.712 ± 0.037	0.378TrpMet: 0.378 ± 0.027	0.552TrpAsn: 0.552 ± 0.038	0.228TrpPro: 0.228 ± 0.021	0.252TrpGln: 0.252 ± 0.02	0.402TrpArg: 0.402 ± 0.027	0.564TrpSer: 0.564 ± 0.034	0.585TrpThr: 0.585 ± 0.036	0.68TrpVal: 0.68 ± 0.034	0.127TrpTrp: 0.127 ± 0.017	0.416TrpTyr: 0.416 ± 0.033	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.878TyrAla: 2.878 ± 0.079	0.759TyrCys: 0.759 ± 0.037	2.669TyrAsp: 2.669 ± 0.086	2.161TyrGlu: 2.161 ± 0.062	1.31TyrPhe: 1.31 ± 0.047	3.104TyrGly: 3.104 ± 0.088	0.68TyrHis: 0.68 ± 0.037	2.121TyrIle: 2.121 ± 0.069	1.726TyrLys: 1.726 ± 0.059	3.262TyrLeu: 3.262 ± 0.076	0.941TyrMet: 0.941 ± 0.048	1.587TyrAsn: 1.587 ± 0.063	1.456TyrPro: 1.456 ± 0.055	0.955TyrGln: 0.955 ± 0.041	2.173TyrArg: 2.173 ± 0.065	2.765TyrSer: 2.765 ± 0.103	2.512TyrThr: 2.512 ± 0.114	2.502TyrVal: 2.502 ± 0.078	0.405TyrTrp: 0.405 ± 0.028	1.536TyrTyr: 1.536 ± 0.063	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1805 proteins (574731 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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