Shrew hepatitis B virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Blubervirales; Hepadnaviridae; Orthohepadnavirus; unclassified Orthohepadnavirus

Average proteome isoelectric point is 8.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481MR26|A0A481MR26_9HEPA Large envelope protein OS=Shrew hepatitis B virus OX=2507572 PE=4 SV=1
MM1 pKa = 7.38GNAVDD6 pKa = 5.29LLGSSLAAGQSYY18 pKa = 9.29GASQGEE24 pKa = 4.4DD25 pKa = 3.67FNPLMDD31 pKa = 5.49LGRR34 pKa = 11.84LLSKK38 pKa = 10.32VNPYY42 pKa = 10.6SRR44 pKa = 11.84LDD46 pKa = 3.54TYY48 pKa = 11.66GDD50 pKa = 3.36WDD52 pKa = 4.08LKK54 pKa = 10.53RR55 pKa = 11.84GNYY58 pKa = 8.75YY59 pKa = 10.29QDD61 pKa = 3.04TSILQGQGQKK71 pKa = 9.83PRR73 pKa = 11.84PPVTPVQPPSPPTRR87 pKa = 11.84GPNRR91 pKa = 11.84VPPLKK96 pKa = 10.23PPQPGTKK103 pKa = 9.41RR104 pKa = 11.84LPINSSQVFPTSSMLRR120 pKa = 11.84PDD122 pKa = 3.22HH123 pKa = 7.06HH124 pKa = 6.52ITPLSKK130 pKa = 10.31EE131 pKa = 4.14IKK133 pKa = 10.0NPPPVPSSASKK144 pKa = 10.91LLVPVEE150 pKa = 4.5NSASSPSLEE159 pKa = 4.1KK160 pKa = 10.54TEE162 pKa = 3.89IGAPVLEE169 pKa = 4.74AMTAEE174 pKa = 4.22PASRR178 pKa = 11.84FLGPLGGLPVVLFLWTRR195 pKa = 11.84IQEE198 pKa = 4.08ILQNLDD204 pKa = 3.05WWWTSLSFPGVSRR217 pKa = 11.84EE218 pKa = 4.08CPGLDD223 pKa = 3.33SQSQTSRR230 pKa = 11.84HH231 pKa = 5.84SLTSCPPTCSGYY243 pKa = 9.73PWMCRR248 pKa = 11.84RR249 pKa = 11.84RR250 pKa = 11.84FIILLCLLLLCLTCWLGYY268 pKa = 10.31LDD270 pKa = 4.8YY271 pKa = 11.3SGEE274 pKa = 4.2LPVCPLGMSGEE285 pKa = 4.4VTTVQCKK292 pKa = 10.15SCTASATGTLGKK304 pKa = 9.19PLCCCLKK311 pKa = 10.76SSGGGNCTCVPIPPSWAFAKK331 pKa = 9.63FLWEE335 pKa = 3.98LVSHH339 pKa = 6.43HH340 pKa = 6.31FSWLSSLLRR349 pKa = 11.84LFQWLEE355 pKa = 4.41GISPTVLLLAIWMIWYY371 pKa = 7.52WVPSLSTIFNLFIPLCLTLWYY392 pKa = 9.75IWGG395 pKa = 3.79

Molecular weight: 43.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481MR21|A0A481MR21_9HEPA Core protein OS=Shrew hepatitis B virus OX=2507572 PE=4 SV=1
MM1 pKa = 7.49AARR4 pKa = 11.84MLFDD8 pKa = 5.96LDD10 pKa = 3.89PATGAVRR17 pKa = 11.84LRR19 pKa = 11.84PFLTEE24 pKa = 3.31PRR26 pKa = 11.84GRR28 pKa = 11.84GEE30 pKa = 3.66QTPRR34 pKa = 11.84PTSSPTTSALSSFLGSRR51 pKa = 11.84SSWRR55 pKa = 11.84RR56 pKa = 11.84LPSCADD62 pKa = 3.5SPFGPCTLRR71 pKa = 11.84FTFAEE76 pKa = 4.38LGNLQTPMNSVTFISCRR93 pKa = 11.84SRR95 pKa = 11.84GAHH98 pKa = 5.6LKK100 pKa = 10.31CRR102 pKa = 11.84RR103 pKa = 11.84QQKK106 pKa = 8.65NWTWYY111 pKa = 9.59FWTHH115 pKa = 5.35HH116 pKa = 5.7NANNTHH122 pKa = 6.34HH123 pKa = 7.18LWLMCYY129 pKa = 10.08GGCRR133 pKa = 11.84HH134 pKa = 6.37KK135 pKa = 11.1

Molecular weight: 15.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1544

135

834

386.0

43.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.505 ± 0.832

2.85 ± 0.624

3.109 ± 0.384

3.238 ± 0.207

4.728 ± 0.685

6.93 ± 0.264

4.08 ± 1.116

3.303 ± 0.54

3.886 ± 0.545

12.565 ± 0.826

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.684 ± 0.274

3.109 ± 0.277

8.161 ± 1.166

4.663 ± 0.557

5.764 ± 1.292

9.909 ± 0.882

5.894 ± 1.017

4.922 ± 0.715

2.785 ± 0.56

2.915 ± 0.459
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski