Amino acid dipepetide frequency for Thiobacillus denitrificans (strain ATCC 25259)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.886AlaAla: 18.886 ± 0.221	1.328AlaCys: 1.328 ± 0.036	7.112AlaAsp: 7.112 ± 0.096	7.684AlaGlu: 7.684 ± 0.108	4.066AlaPhe: 4.066 ± 0.07	10.86AlaGly: 10.86 ± 0.146	2.905AlaHis: 2.905 ± 0.064	5.23AlaIle: 5.23 ± 0.08	3.672AlaLys: 3.672 ± 0.085	15.187AlaLeu: 15.187 ± 0.165	3.124AlaMet: 3.124 ± 0.072	2.723AlaAsn: 2.723 ± 0.068	6.175AlaPro: 6.175 ± 0.097	4.808AlaGln: 4.808 ± 0.085	10.517AlaArg: 10.517 ± 0.141	6.307AlaSer: 6.307 ± 0.096	5.631AlaThr: 5.631 ± 0.08	9.175AlaVal: 9.175 ± 0.104	1.89AlaTrp: 1.89 ± 0.052	2.824AlaTyr: 2.824 ± 0.064	0.0AlaXaa: 0.0 ± 0.0
Cys	1.094CysAla: 1.094 ± 0.036	0.129CysCys: 0.129 ± 0.012	0.493CysAsp: 0.493 ± 0.025	0.492CysGlu: 0.492 ± 0.026	0.24CysPhe: 0.24 ± 0.016	0.919CysGly: 0.919 ± 0.032	0.294CysHis: 0.294 ± 0.02	0.349CysIle: 0.349 ± 0.018	0.266CysLys: 0.266 ± 0.017	0.78CysLeu: 0.78 ± 0.031	0.195CysMet: 0.195 ± 0.015	0.242CysAsn: 0.242 ± 0.017	0.596CysPro: 0.596 ± 0.028	0.258CysGln: 0.258 ± 0.017	0.687CysArg: 0.687 ± 0.028	0.465CysSer: 0.465 ± 0.025	0.437CysThr: 0.437 ± 0.022	0.664CysVal: 0.664 ± 0.027	0.084CysTrp: 0.084 ± 0.011	0.24CysTyr: 0.24 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	8.151AspAla: 8.151 ± 0.119	0.469AspCys: 0.469 ± 0.023	3.145AspAsp: 3.145 ± 0.075	3.493AspGlu: 3.493 ± 0.073	2.289AspPhe: 2.289 ± 0.058	4.468AspGly: 4.468 ± 0.088	1.141AspHis: 1.141 ± 0.038	2.468AspIle: 2.468 ± 0.054	1.83AspLys: 1.83 ± 0.054	5.635AspLeu: 5.635 ± 0.087	1.191AspMet: 1.191 ± 0.037	1.268AspAsn: 1.268 ± 0.037	3.151AspPro: 3.151 ± 0.063	1.419AspGln: 1.419 ± 0.034	3.561AspArg: 3.561 ± 0.064	2.408AspSer: 2.408 ± 0.052	2.86AspThr: 2.86 ± 0.065	4.278AspVal: 4.278 ± 0.077	1.002AspTrp: 1.002 ± 0.036	1.655AspTyr: 1.655 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	7.957GluAla: 7.957 ± 0.098	0.416GluCys: 0.416 ± 0.022	2.475GluAsp: 2.475 ± 0.056	2.644GluGlu: 2.644 ± 0.07	1.932GluPhe: 1.932 ± 0.048	3.812GluGly: 3.812 ± 0.068	1.432GluHis: 1.432 ± 0.041	3.238GluIle: 3.238 ± 0.07	2.307GluLys: 2.307 ± 0.055	6.205GluLeu: 6.205 ± 0.099	1.405GluMet: 1.405 ± 0.041	1.561GluAsn: 1.561 ± 0.043	2.457GluPro: 2.457 ± 0.062	2.306GluGln: 2.306 ± 0.054	4.92GluArg: 4.92 ± 0.088	2.787GluSer: 2.787 ± 0.065	3.342GluThr: 3.342 ± 0.067	4.205GluVal: 4.205 ± 0.082	0.727GluTrp: 0.727 ± 0.028	1.244GluTyr: 1.244 ± 0.039	0.0GluXaa: 0.0 ± 0.0
Phe	4.309PheAla: 4.309 ± 0.069	0.386PheCys: 0.386 ± 0.021	2.585PheAsp: 2.585 ± 0.061	2.153PheGlu: 2.153 ± 0.047	1.405PhePhe: 1.405 ± 0.041	3.16PheGly: 3.16 ± 0.06	0.808PheHis: 0.808 ± 0.025	1.566PheIle: 1.566 ± 0.041	1.08PheLys: 1.08 ± 0.041	3.235PheLeu: 3.235 ± 0.067	0.791PheMet: 0.791 ± 0.028	1.07PheAsn: 1.07 ± 0.032	1.604PhePro: 1.604 ± 0.042	0.914PheGln: 0.914 ± 0.035	2.208PheArg: 2.208 ± 0.061	2.116PheSer: 2.116 ± 0.048	1.786PheThr: 1.786 ± 0.041	3.004PheVal: 3.004 ± 0.057	0.46PheTrp: 0.46 ± 0.025	0.969PheTyr: 0.969 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	8.84GlyAla: 8.84 ± 0.119	0.91GlyCys: 0.91 ± 0.037	4.357GlyAsp: 4.357 ± 0.093	5.031GlyGlu: 5.031 ± 0.073	3.349GlyPhe: 3.349 ± 0.063	6.743GlyGly: 6.743 ± 0.128	1.902GlyHis: 1.902 ± 0.047	3.903GlyIle: 3.903 ± 0.064	3.268GlyLys: 3.268 ± 0.065	8.58GlyLeu: 8.58 ± 0.108	2.213GlyMet: 2.213 ± 0.053	2.122GlyAsn: 2.122 ± 0.055	2.552GlyPro: 2.552 ± 0.058	2.826GlyGln: 2.826 ± 0.054	6.1GlyArg: 6.1 ± 0.097	4.13GlySer: 4.13 ± 0.074	3.894GlyThr: 3.894 ± 0.078	6.686GlyVal: 6.686 ± 0.096	1.31GlyTrp: 1.31 ± 0.044	2.371GlyTyr: 2.371 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	3.092HisAla: 3.092 ± 0.062	0.276HisCys: 0.276 ± 0.018	1.294HisAsp: 1.294 ± 0.041	1.271HisGlu: 1.271 ± 0.035	0.957HisPhe: 0.957 ± 0.035	2.207HisGly: 2.207 ± 0.056	0.649HisHis: 0.649 ± 0.03	0.895HisIle: 0.895 ± 0.035	0.605HisLys: 0.605 ± 0.027	2.364HisLeu: 2.364 ± 0.056	0.47HisMet: 0.47 ± 0.023	0.491HisAsn: 0.491 ± 0.022	1.644HisPro: 1.644 ± 0.044	0.618HisGln: 0.618 ± 0.029	1.569HisArg: 1.569 ± 0.046	0.988HisSer: 0.988 ± 0.038	1.089HisThr: 1.089 ± 0.034	1.693HisVal: 1.693 ± 0.044	0.358HisTrp: 0.358 ± 0.021	0.742HisTyr: 0.742 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.926IleAla: 5.926 ± 0.085	0.369IleCys: 0.369 ± 0.02	3.065IleAsp: 3.065 ± 0.063	3.21IleGlu: 3.21 ± 0.063	1.313IlePhe: 1.313 ± 0.043	3.812IleGly: 3.812 ± 0.068	0.97IleHis: 0.97 ± 0.032	1.467IleIle: 1.467 ± 0.045	1.517IleLys: 1.517 ± 0.047	3.922IleLeu: 3.922 ± 0.068	0.781IleMet: 0.781 ± 0.031	1.262IleAsn: 1.262 ± 0.037	2.277IlePro: 2.277 ± 0.05	1.276IleGln: 1.276 ± 0.042	3.004IleArg: 3.004 ± 0.061	2.134IleSer: 2.134 ± 0.044	2.192IleThr: 2.192 ± 0.049	3.644IleVal: 3.644 ± 0.072	0.429IleTrp: 0.429 ± 0.026	1.007IleTyr: 1.007 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	3.718LysAla: 3.718 ± 0.092	0.186LysCys: 0.186 ± 0.015	1.673LysAsp: 1.673 ± 0.045	1.631LysGlu: 1.631 ± 0.051	0.923LysPhe: 0.923 ± 0.035	2.367LysGly: 2.367 ± 0.05	0.736LysHis: 0.736 ± 0.032	1.603LysIle: 1.603 ± 0.043	1.533LysLys: 1.533 ± 0.058	3.653LysLeu: 3.653 ± 0.076	0.848LysMet: 0.848 ± 0.033	1.046LysAsn: 1.046 ± 0.039	2.047LysPro: 2.047 ± 0.055	1.366LysGln: 1.366 ± 0.044	2.515LysArg: 2.515 ± 0.07	1.774LysSer: 1.774 ± 0.045	2.075LysThr: 2.075 ± 0.045	2.322LysVal: 2.322 ± 0.058	0.395LysTrp: 0.395 ± 0.021	0.731LysTyr: 0.731 ± 0.034	0.0LysXaa: 0.0 ± 0.0
Leu	16.06LeuAla: 16.06 ± 0.169	0.801LeuCys: 0.801 ± 0.031	6.865LeuAsp: 6.865 ± 0.094	5.898LeuGlu: 5.898 ± 0.092	3.72LeuPhe: 3.72 ± 0.067	8.767LeuGly: 8.767 ± 0.099	2.476LeuHis: 2.476 ± 0.053	4.4LeuIle: 4.4 ± 0.087	3.987LeuLys: 3.987 ± 0.079	11.646LeuLeu: 11.646 ± 0.172	2.338LeuMet: 2.338 ± 0.052	2.659LeuAsn: 2.659 ± 0.063	6.245LeuPro: 6.245 ± 0.087	3.362LeuGln: 3.362 ± 0.063	7.723LeuArg: 7.723 ± 0.1	5.783LeuSer: 5.783 ± 0.077	5.512LeuThr: 5.512 ± 0.09	7.997LeuVal: 7.997 ± 0.109	1.25LeuTrp: 1.25 ± 0.038	2.232LeuTyr: 2.232 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	2.655MetAla: 2.655 ± 0.062	0.166MetCys: 0.166 ± 0.014	1.108MetAsp: 1.108 ± 0.043	1.085MetGlu: 1.085 ± 0.037	0.679MetPhe: 0.679 ± 0.027	1.585MetGly: 1.585 ± 0.042	0.532MetHis: 0.532 ± 0.025	0.954MetIle: 0.954 ± 0.035	1.071MetLys: 1.071 ± 0.035	2.59MetLeu: 2.59 ± 0.058	0.597MetMet: 0.597 ± 0.03	0.871MetAsn: 0.871 ± 0.033	1.278MetPro: 1.278 ± 0.038	1.02MetGln: 1.02 ± 0.036	1.801MetArg: 1.801 ± 0.049	1.577MetSer: 1.577 ± 0.045	1.409MetThr: 1.409 ± 0.042	1.488MetVal: 1.488 ± 0.043	0.193MetTrp: 0.193 ± 0.015	0.358MetTyr: 0.358 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.065AsnAla: 3.065 ± 0.073	0.282AsnCys: 0.282 ± 0.02	1.278AsnAsp: 1.278 ± 0.037	1.283AsnGlu: 1.283 ± 0.034	0.984AsnPhe: 0.984 ± 0.03	2.076AsnGly: 2.076 ± 0.056	0.51AsnHis: 0.51 ± 0.021	1.165AsnIle: 1.165 ± 0.037	0.818AsnLys: 0.818 ± 0.035	2.911AsnLeu: 2.911 ± 0.064	0.55AsnMet: 0.55 ± 0.025	0.673AsnAsn: 0.673 ± 0.032	1.826AsnPro: 1.826 ± 0.057	0.802AsnGln: 0.802 ± 0.032	1.835AsnArg: 1.835 ± 0.047	1.074AsnSer: 1.074 ± 0.033	1.268AsnThr: 1.268 ± 0.04	1.907AsnVal: 1.907 ± 0.049	0.414AsnTrp: 0.414 ± 0.024	0.664AsnTyr: 0.664 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	7.09ProAla: 7.09 ± 0.112	0.393ProCys: 0.393 ± 0.022	3.278ProAsp: 3.278 ± 0.064	3.532ProGlu: 3.532 ± 0.067	1.744ProPhe: 1.744 ± 0.045	4.595ProGly: 4.595 ± 0.072	1.192ProHis: 1.192 ± 0.038	1.946ProIle: 1.946 ± 0.048	1.369ProLys: 1.369 ± 0.043	5.322ProLeu: 5.322 ± 0.071	1.089ProMet: 1.089 ± 0.038	1.237ProAsn: 1.237 ± 0.037	2.768ProPro: 2.768 ± 0.077	1.766ProGln: 1.766 ± 0.054	3.285ProArg: 3.285 ± 0.078	2.364ProSer: 2.364 ± 0.055	2.358ProThr: 2.358 ± 0.05	4.052ProVal: 4.052 ± 0.072	0.704ProTrp: 0.704 ± 0.03	1.238ProTyr: 1.238 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	4.905GlnAla: 4.905 ± 0.085	0.276GlnCys: 0.276 ± 0.017	1.481GlnAsp: 1.481 ± 0.048	1.55GlnGlu: 1.55 ± 0.04	1.148GlnPhe: 1.148 ± 0.037	2.49GlnGly: 2.49 ± 0.059	0.83GlnHis: 0.83 ± 0.029	1.693GlnIle: 1.693 ± 0.043	1.221GlnLys: 1.221 ± 0.045	3.69GlnLeu: 3.69 ± 0.072	0.813GlnMet: 0.813 ± 0.028	0.799GlnAsn: 0.799 ± 0.028	1.879GlnPro: 1.879 ± 0.047	1.499GlnGln: 1.499 ± 0.047	2.694GlnArg: 2.694 ± 0.06	1.728GlnSer: 1.728 ± 0.042	1.775GlnThr: 1.775 ± 0.045	2.565GlnVal: 2.565 ± 0.055	0.405GlnTrp: 0.405 ± 0.023	0.752GlnTyr: 0.752 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	8.913ArgAla: 8.913 ± 0.13	0.606ArgCys: 0.606 ± 0.028	4.142ArgAsp: 4.142 ± 0.068	4.735ArgGlu: 4.735 ± 0.082	2.993ArgPhe: 2.993 ± 0.064	5.1ArgGly: 5.1 ± 0.077	2.015ArgHis: 2.015 ± 0.048	3.623ArgIle: 3.623 ± 0.065	2.109ArgLys: 2.109 ± 0.054	8.754ArgLeu: 8.754 ± 0.126	1.829ArgMet: 1.829 ± 0.051	1.888ArgAsn: 1.888 ± 0.05	3.441ArgPro: 3.441 ± 0.066	2.911ArgGln: 2.911 ± 0.06	5.929ArgArg: 5.929 ± 0.102	3.283ArgSer: 3.283 ± 0.067	3.114ArgThr: 3.114 ± 0.062	5.629ArgVal: 5.629 ± 0.088	1.067ArgTrp: 1.067 ± 0.037	2.188ArgTyr: 2.188 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	5.912SerAla: 5.912 ± 0.082	0.441SerCys: 0.441 ± 0.023	2.68SerAsp: 2.68 ± 0.049	2.818SerGlu: 2.818 ± 0.061	1.904SerPhe: 1.904 ± 0.051	5.187SerGly: 5.187 ± 0.086	1.136SerHis: 1.136 ± 0.038	2.113SerIle: 2.113 ± 0.049	1.495SerLys: 1.495 ± 0.044	5.462SerLeu: 5.462 ± 0.092	1.206SerMet: 1.206 ± 0.04	1.219SerAsn: 1.219 ± 0.039	2.586SerPro: 2.586 ± 0.059	1.55SerGln: 1.55 ± 0.048	3.605SerArg: 3.605 ± 0.065	2.401SerSer: 2.401 ± 0.061	2.451SerThr: 2.451 ± 0.053	3.667SerVal: 3.667 ± 0.071	0.639SerTrp: 0.639 ± 0.026	1.168SerTyr: 1.168 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	5.663ThrAla: 5.663 ± 0.088	0.416ThrCys: 0.416 ± 0.024	2.414ThrAsp: 2.414 ± 0.052	2.436ThrGlu: 2.436 ± 0.058	1.512ThrPhe: 1.512 ± 0.043	4.4ThrGly: 4.4 ± 0.091	1.186ThrHis: 1.186 ± 0.038	2.021ThrIle: 2.021 ± 0.05	1.159ThrLys: 1.159 ± 0.038	6.825ThrLeu: 6.825 ± 0.1	0.929ThrMet: 0.929 ± 0.034	1.056ThrAsn: 1.056 ± 0.039	3.35ThrPro: 3.35 ± 0.067	1.645ThrGln: 1.645 ± 0.043	3.868ThrArg: 3.868 ± 0.065	2.21ThrSer: 2.21 ± 0.051	2.495ThrThr: 2.495 ± 0.066	4.05ThrVal: 4.05 ± 0.068	0.614ThrTrp: 0.614 ± 0.032	1.116ThrTyr: 1.116 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	9.723ValAla: 9.723 ± 0.114	0.702ValCys: 0.702 ± 0.033	4.502ValAsp: 4.502 ± 0.077	4.576ValGlu: 4.576 ± 0.082	2.83ValPhe: 2.83 ± 0.064	5.592ValGly: 5.592 ± 0.077	1.614ValHis: 1.614 ± 0.045	3.506ValIle: 3.506 ± 0.065	2.707ValLys: 2.707 ± 0.063	8.159ValLeu: 8.159 ± 0.111	1.835ValMet: 1.835 ± 0.046	2.149ValAsn: 2.149 ± 0.051	3.545ValPro: 3.545 ± 0.063	2.213ValGln: 2.213 ± 0.045	5.195ValArg: 5.195 ± 0.077	4.221ValSer: 4.221 ± 0.066	3.92ValThr: 3.92 ± 0.072	6.526ValVal: 6.526 ± 0.108	0.965ValTrp: 0.965 ± 0.038	1.83ValTyr: 1.83 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	1.263TrpAla: 1.263 ± 0.042	0.134TrpCys: 0.134 ± 0.013	0.638TrpAsp: 0.638 ± 0.027	0.603TrpGlu: 0.603 ± 0.025	0.522TrpPhe: 0.522 ± 0.024	0.846TrpGly: 0.846 ± 0.035	0.393TrpHis: 0.393 ± 0.023	0.636TrpIle: 0.636 ± 0.031	0.446TrpLys: 0.446 ± 0.022	2.038TrpLeu: 2.038 ± 0.053	0.369TrpMet: 0.369 ± 0.022	0.428TrpAsn: 0.428 ± 0.024	0.614TrpPro: 0.614 ± 0.028	0.689TrpGln: 0.689 ± 0.026	1.203TrpArg: 1.203 ± 0.048	0.613TrpSer: 0.613 ± 0.026	0.568TrpThr: 0.568 ± 0.028	0.995TrpVal: 0.995 ± 0.036	0.225TrpTrp: 0.225 ± 0.018	0.291TrpTyr: 0.291 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.882TyrAla: 2.882 ± 0.06	0.24TyrCys: 0.24 ± 0.018	1.299TyrAsp: 1.299 ± 0.036	1.248TyrGlu: 1.248 ± 0.038	1.098TyrPhe: 1.098 ± 0.033	2.105TyrGly: 2.105 ± 0.055	0.553TyrHis: 0.553 ± 0.023	0.799TyrIle: 0.799 ± 0.032	0.678TyrLys: 0.678 ± 0.028	2.764TyrLeu: 2.764 ± 0.061	0.445TyrMet: 0.445 ± 0.024	0.623TyrAsn: 0.623 ± 0.028	1.285TyrPro: 1.285 ± 0.038	0.942TyrGln: 0.942 ± 0.034	2.05TyrArg: 2.05 ± 0.051	1.276TyrSer: 1.276 ± 0.043	1.187TyrThr: 1.187 ± 0.038	1.763TyrVal: 1.763 ± 0.04	0.384TyrTrp: 0.384 ± 0.024	0.678TyrTyr: 0.678 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2826 proteins (894101 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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