Nocardioides ganghwensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q2SFR5|A0A4Q2SFR5_9ACTN Signal recognition particle protein OS=Nocardioides ganghwensis OX=252230 GN=ffh PE=3 SV=1
MM1 pKa = 7.01TALSAVVGACGGDD14 pKa = 3.66DD15 pKa = 3.82ADD17 pKa = 4.14GTSVQDD23 pKa = 3.72FMEE26 pKa = 4.36SAGQVRR32 pKa = 11.84AITVSDD38 pKa = 3.27IYY40 pKa = 11.44DD41 pKa = 3.57RR42 pKa = 11.84DD43 pKa = 3.62VSDD46 pKa = 3.8VVIVCAYY53 pKa = 9.04TGATAITDD61 pKa = 3.51EE62 pKa = 5.85LGFDD66 pKa = 3.52WPGATSLAKK75 pKa = 10.3QVDD78 pKa = 4.06TSDD81 pKa = 2.9SHH83 pKa = 5.56QAVIAVHH90 pKa = 6.1HH91 pKa = 6.3GEE93 pKa = 4.28VVEE96 pKa = 4.39SEE98 pKa = 4.31VMLIDD103 pKa = 3.59VLYY106 pKa = 10.7VCDD109 pKa = 4.49MDD111 pKa = 4.55LAYY114 pKa = 9.45PATIDD119 pKa = 3.74AGTTLKK125 pKa = 10.94VSWSSTAWSDD135 pKa = 3.39GTTKK139 pKa = 9.83TVPVASVDD147 pKa = 3.34

Molecular weight: 15.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q2SCW4|A0A4Q2SCW4_9ACTN GntR family transcriptional regulator OS=Nocardioides ganghwensis OX=252230 GN=EUA07_14765 PE=4 SV=1
MM1 pKa = 7.09GRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84ARR7 pKa = 11.84RR8 pKa = 11.84HH9 pKa = 5.18GPAPRR14 pKa = 11.84PRR16 pKa = 11.84RR17 pKa = 11.84AGRR20 pKa = 11.84AAAAGRR26 pKa = 11.84PRR28 pKa = 11.84RR29 pKa = 11.84GSAASSPRR37 pKa = 11.84APRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84CWW44 pKa = 3.01

Molecular weight: 4.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4127

0

4127

1368510

27

2352

331.6

35.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.087 ± 0.05

0.71 ± 0.01

6.782 ± 0.031

5.902 ± 0.035

2.736 ± 0.023

9.181 ± 0.037

2.287 ± 0.022

3.216 ± 0.027

1.818 ± 0.028

10.156 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.84 ± 0.016

1.648 ± 0.019

5.639 ± 0.028

2.628 ± 0.019

7.856 ± 0.049

5.241 ± 0.024

6.124 ± 0.037

9.667 ± 0.04

1.616 ± 0.017

1.865 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski