Streptococcus phage Javan393

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AAS4|A0A4D6AAS4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan393 OX=2548146 GN=Javan393_0030 PE=4 SV=1
MM1 pKa = 7.35GVYY4 pKa = 10.4SSLDD8 pKa = 3.14KK9 pKa = 11.39ARR11 pKa = 11.84DD12 pKa = 3.68YY13 pKa = 11.28IDD15 pKa = 3.06WAGYY19 pKa = 9.63RR20 pKa = 11.84YY21 pKa = 10.17EE22 pKa = 5.58DD23 pKa = 4.41GFFQEE28 pKa = 4.17YY29 pKa = 10.4SEE31 pKa = 4.14YY32 pKa = 11.11LEE34 pKa = 4.64TYY36 pKa = 10.42SKK38 pKa = 11.48EE39 pKa = 3.99EE40 pKa = 4.18DD41 pKa = 3.39NGVHH45 pKa = 5.17KK46 pKa = 9.5TISILEE52 pKa = 4.14RR53 pKa = 11.84EE54 pKa = 4.11LDD56 pKa = 3.45KK57 pKa = 11.55DD58 pKa = 3.35LGEE61 pKa = 4.51LVFEE65 pKa = 4.57AVGTEE70 pKa = 3.84

Molecular weight: 8.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A9Q4|A0A4D6A9Q4_9CAUD Antirepressor protein OS=Streptococcus phage Javan393 OX=2548146 GN=Javan393_0055 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.56FILKK6 pKa = 10.0NIHH9 pKa = 6.18TIILLIGIGLVMYY22 pKa = 9.57GFFLISQMVGYY33 pKa = 9.91IGSGVLAIMLATYY46 pKa = 10.16IDD48 pKa = 3.58RR49 pKa = 11.84SVRR52 pKa = 3.37

Molecular weight: 5.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12606

38

1528

210.1

23.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.346 ± 0.608

0.555 ± 0.099

6.497 ± 0.23

7.203 ± 0.468

3.887 ± 0.238

6.426 ± 0.486

1.325 ± 0.174

6.87 ± 0.374

8.774 ± 0.42

8.25 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.246

6.092 ± 0.201

2.808 ± 0.164

3.673 ± 0.226

3.459 ± 0.249

6.735 ± 0.436

7.124 ± 0.425

6.084 ± 0.222

1.198 ± 0.124

4.077 ± 0.277
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski