Pichia kudriavzevii (Yeast) (Issatchenkia orientalis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Pichiaceae; Pichia

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5146 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U9R0P9|A0A2U9R0P9_PICKU Uncharacterized protein OS=Pichia kudriavzevii OX=4909 GN=C5L36_0B00160 PE=4 SV=1
MM1 pKa = 7.5NFQISLIFFTFLQVILAAPLPYY23 pKa = 10.01YY24 pKa = 9.65VTVTAPDD31 pKa = 3.73TYY33 pKa = 9.5VTTIIPVAEE42 pKa = 4.43VIISDD47 pKa = 3.94GQTTTIQLTTLVTQTSPAADD67 pKa = 3.78PTTLATEE74 pKa = 4.48TNLPADD80 pKa = 3.8PTTEE84 pKa = 4.07NTEE87 pKa = 4.28TTPEE91 pKa = 4.11TTVDD95 pKa = 3.4SVTPATSEE103 pKa = 4.3TTSTPALTTSDD114 pKa = 3.94CTSTSISVDD123 pKa = 3.67SNGVAHH129 pKa = 6.72VWVTLDD135 pKa = 3.15KK136 pKa = 10.26TVLVGTDD143 pKa = 3.59GVPFDD148 pKa = 4.33TQTEE152 pKa = 4.17QLPQATEE159 pKa = 4.44TPVSTSSTQTSVDD172 pKa = 3.39LVGNNNVAAATLSTDD187 pKa = 3.4TTSSFTSATTGSTSPTTQSTPNTTNTSSEE216 pKa = 4.29DD217 pKa = 3.39STSAISTTLATEE229 pKa = 4.41TTSSSSAQDD238 pKa = 3.43SSSEE242 pKa = 4.16TSLSMNTDD250 pKa = 2.93AAGKK254 pKa = 9.56DD255 pKa = 3.53KK256 pKa = 11.2PFVTTWANGEE266 pKa = 4.2VFYY269 pKa = 11.41SSLPFSLVPSEE280 pKa = 4.17EE281 pKa = 4.11VAPNVALTTTTLTNINDD298 pKa = 4.05APTTTALLSLVSSSDD313 pKa = 3.34KK314 pKa = 11.12LLLPTLTVGLPKK326 pKa = 10.62DD327 pKa = 3.68KK328 pKa = 11.13NVNTNMADD336 pKa = 3.51PATVSVTNTKK346 pKa = 10.13QVTTPVIVTTSSTSSTTPTADD367 pKa = 3.44STSSSSPDD375 pKa = 3.76DD376 pKa = 3.65VLQDD380 pKa = 3.79EE381 pKa = 4.63QTSTSAASSSASSDD395 pKa = 3.22SGLLTKK401 pKa = 10.5APYY404 pKa = 10.29SIVYY408 pKa = 9.98SPYY411 pKa = 11.23NNDD414 pKa = 3.59NSCKK418 pKa = 10.39SYY420 pKa = 8.0TTVYY424 pKa = 10.58TDD426 pKa = 4.24LKK428 pKa = 10.7LIASKK433 pKa = 10.58GIKK436 pKa = 9.51EE437 pKa = 3.54IRR439 pKa = 11.84IYY441 pKa = 11.33GNDD444 pKa = 3.6CNYY447 pKa = 8.83LTTVLNVANKK457 pKa = 10.45LGLKK461 pKa = 9.43VNQGFWISQDD471 pKa = 3.19GANSIDD477 pKa = 3.64DD478 pKa = 4.02AVDD481 pKa = 3.21NFITYY486 pKa = 9.85ISSGAAEE493 pKa = 4.22YY494 pKa = 10.7SWDD497 pKa = 3.68LFSYY501 pKa = 8.83ITVGNEE507 pKa = 3.93AIISNYY513 pKa = 9.87CSVDD517 pKa = 3.37DD518 pKa = 5.32LINKK522 pKa = 9.41ISEE525 pKa = 4.37VKK527 pKa = 10.67GKK529 pKa = 10.51LNAAGYY535 pKa = 8.91SGKK538 pKa = 8.4ITTSEE543 pKa = 4.05PPVSFEE549 pKa = 5.56NNPQLCTDD557 pKa = 3.74SEE559 pKa = 4.34IDD561 pKa = 3.56FVGINPHH568 pKa = 6.42SYY570 pKa = 10.21FDD572 pKa = 4.09VYY574 pKa = 11.37SSADD578 pKa = 3.32NSGVFVAGQIEE589 pKa = 4.67IVKK592 pKa = 9.24QYY594 pKa = 10.84CGDD597 pKa = 3.39KK598 pKa = 10.75DD599 pKa = 3.6IVVTEE604 pKa = 4.13TGYY607 pKa = 10.28PSAGNVNGDD616 pKa = 3.31NVPSVEE622 pKa = 4.05NQRR625 pKa = 11.84IAVQSILDD633 pKa = 3.71VVGTDD638 pKa = 3.29VTILSTYY645 pKa = 10.62DD646 pKa = 4.76DD647 pKa = 4.05YY648 pKa = 11.61WKK650 pKa = 10.75QPGPYY655 pKa = 9.74NIEE658 pKa = 3.64QHH660 pKa = 6.21FGIIQLLPSVV670 pKa = 4.02

Molecular weight: 70.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U9RAN9|A0A2U9RAN9_PICKU TUG-UBL1 domain-containing protein OS=Pichia kudriavzevii OX=4909 GN=C5L36_0E05320 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.09WRR6 pKa = 11.84KK7 pKa = 8.87KK8 pKa = 8.73RR9 pKa = 11.84VRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.71RR15 pKa = 11.84KK16 pKa = 8.27RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.19VRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 10.98

Molecular weight: 3.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5146

0

5146

2636562

25

5008

512.4

57.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.233 ± 0.031

1.244 ± 0.012

5.993 ± 0.025

6.848 ± 0.032

4.442 ± 0.024

5.195 ± 0.027

2.136 ± 0.012

6.702 ± 0.022

7.348 ± 0.034

9.516 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.15 ± 0.012

6.111 ± 0.035

4.196 ± 0.025

3.792 ± 0.028

4.34 ± 0.021

8.77 ± 0.048

5.65 ± 0.028

5.83 ± 0.024

0.981 ± 0.009

3.525 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski