Tahibacter aquaticus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Rhodanobacteraceae; Tahibacter

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5664 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6YSH2|A0A4R6YSH2_9GAMM Diguanylate cyclase (GGDEF)-like protein OS=Tahibacter aquaticus OX=520092 GN=DFR29_111113 PE=4 SV=1
MM1 pKa = 8.0PSTALPRR8 pKa = 11.84LVRR11 pKa = 11.84AWLLISLLSCLPSLAFAQCVSLTTLGSAVTQNFDD45 pKa = 3.8TLSNTAGSTTNNLTITGWFLTEE67 pKa = 4.01SGGGARR73 pKa = 11.84DD74 pKa = 3.55NEE76 pKa = 4.58QYY78 pKa = 11.27GVDD81 pKa = 3.67TGASTTGDD89 pKa = 3.37MYY91 pKa = 11.42SYY93 pKa = 10.86GAAAATDD100 pKa = 3.6RR101 pKa = 11.84ALGQLRR107 pKa = 11.84SGTLIPLFGACFTNNTGSTLASLAVAYY134 pKa = 9.22TGEE137 pKa = 4.12EE138 pKa = 3.98WRR140 pKa = 11.84LGTAARR146 pKa = 11.84TDD148 pKa = 3.64TLAFEE153 pKa = 4.93YY154 pKa = 9.79STTATDD160 pKa = 4.1LVTGAWTGVTALNFVTPDD178 pKa = 3.13TATAGAKK185 pKa = 9.83NGNAAADD192 pKa = 4.1RR193 pKa = 11.84TALASTISGLSIANGASFWIRR214 pKa = 11.84WNDD217 pKa = 3.05TDD219 pKa = 5.77ASGADD224 pKa = 3.97DD225 pKa = 4.92GLAVDD230 pKa = 5.47DD231 pKa = 6.36FSLTPQAPIALPNLSINDD249 pKa = 3.65VILNEE254 pKa = 4.27GNAGTTSFTFTVSLSAPAGPGGVTFDD280 pKa = 3.47IATADD285 pKa = 3.74GTAVAPGDD293 pKa = 4.07YY294 pKa = 8.81VTKK297 pKa = 10.61SLTSQTIPAGSSTYY311 pKa = 11.1SFTVLANGDD320 pKa = 3.79TTPEE324 pKa = 3.95TNEE327 pKa = 3.95TFFVNVTAATGATVTDD343 pKa = 4.34GQGQGTLVNDD353 pKa = 4.13DD354 pKa = 4.11AAPNLTINDD363 pKa = 3.23VSLNEE368 pKa = 4.19GNAGTTTFTFIASLSAPAPAGGVTFDD394 pKa = 3.23IATANGTAIAPGDD407 pKa = 3.8YY408 pKa = 8.67TASSLTGQTIPAGSSTYY425 pKa = 9.85TFSVLVNGDD434 pKa = 3.33ITPEE438 pKa = 3.75SDD440 pKa = 2.91EE441 pKa = 4.49TFVVTITNATNAIVTDD457 pKa = 4.24GQGQGNIVNDD467 pKa = 4.22DD468 pKa = 3.8LSRR471 pKa = 11.84IHH473 pKa = 7.31DD474 pKa = 3.75VQGNGASTPIPGATVTVQGIVIGNFQGAGRR504 pKa = 11.84LQGFFLQEE512 pKa = 3.24ADD514 pKa = 4.01ANADD518 pKa = 3.49ADD520 pKa = 4.08PATSEE525 pKa = 4.85GIFVSCGSCATPVAEE540 pKa = 4.33GQVVRR545 pKa = 11.84ATGVVSEE552 pKa = 4.97SNNLTQISATTAGAVVVTNAGNNLAQVTPTTIDD585 pKa = 3.57LPVVGAIDD593 pKa = 3.88DD594 pKa = 4.41FYY596 pKa = 11.55EE597 pKa = 4.28SRR599 pKa = 11.84EE600 pKa = 4.1SMRR603 pKa = 11.84VTFVDD608 pKa = 3.67TLAVSDD614 pKa = 4.18YY615 pKa = 11.39FEE617 pKa = 4.08MARR620 pKa = 11.84YY621 pKa = 7.99GQLVLMEE628 pKa = 4.71GGRR631 pKa = 11.84PRR633 pKa = 11.84AFTEE637 pKa = 4.15FATPSVAGYY646 pKa = 7.38TAYY649 pKa = 11.05LDD651 pKa = 3.32NLARR655 pKa = 11.84RR656 pKa = 11.84RR657 pKa = 11.84VLLDD661 pKa = 3.92DD662 pKa = 5.46DD663 pKa = 5.4NNAQEE668 pKa = 5.1SFLTQPNGMQSVYY681 pKa = 10.42HH682 pKa = 6.07PRR684 pKa = 11.84ANGGFSFGTQGTDD697 pKa = 3.28FFRR700 pKa = 11.84GGDD703 pKa = 3.8LVNGLTGVLDD713 pKa = 4.54FSDD716 pKa = 4.11PAGAASPSWRR726 pKa = 11.84VRR728 pKa = 11.84PVAATPPTFTVANPRR743 pKa = 11.84PATPPAVGGAIKK755 pKa = 9.7VTGLNLLNYY764 pKa = 7.46FTTIDD769 pKa = 3.57TTASSSAGPCGPGGTLDD786 pKa = 3.76CRR788 pKa = 11.84GADD791 pKa = 3.35SVAEE795 pKa = 4.12LNRR798 pKa = 11.84QRR800 pKa = 11.84EE801 pKa = 4.13RR802 pKa = 11.84ASVVICSLNADD813 pKa = 3.46VAALIEE819 pKa = 4.5LEE821 pKa = 4.19NTTPSATITDD831 pKa = 4.12LLGAINARR839 pKa = 11.84CGGAHH844 pKa = 7.4PYY846 pKa = 10.96AFANTGGTLGTDD858 pKa = 4.58AIRR861 pKa = 11.84VQPIYY866 pKa = 9.62RR867 pKa = 11.84TGIVSPVGSPLSDD880 pKa = 4.2LDD882 pKa = 3.89PVHH885 pKa = 6.41NRR887 pKa = 11.84PPTAQTFDD895 pKa = 3.52VVDD898 pKa = 3.55ATNAAFGQRR907 pKa = 11.84FTVIANHH914 pKa = 6.22LRR916 pKa = 11.84AKK918 pKa = 9.68SCSGATGGDD927 pKa = 3.7ADD929 pKa = 4.18TGDD932 pKa = 3.83GQSCFTAARR941 pKa = 11.84TAQATRR947 pKa = 11.84LLSWISSTVIPAAGDD962 pKa = 3.61PDD964 pKa = 4.2VLLLGDD970 pKa = 4.06FNAYY974 pKa = 9.69AQEE977 pKa = 4.26TPIATIVAGGYY988 pKa = 8.39TDD990 pKa = 5.78LEE992 pKa = 4.64TAFEE996 pKa = 4.62GPAAYY1001 pKa = 10.08SYY1003 pKa = 11.71VFGGALGHH1011 pKa = 7.2LDD1013 pKa = 3.56YY1014 pKa = 11.52AFASASLNSQVIGASAWHH1032 pKa = 6.47INADD1036 pKa = 4.01EE1037 pKa = 4.47VPLLDD1042 pKa = 4.65YY1043 pKa = 10.92NDD1045 pKa = 3.77EE1046 pKa = 4.19VADD1049 pKa = 3.74VGEE1052 pKa = 4.51SAFEE1056 pKa = 3.98EE1057 pKa = 4.66KK1058 pKa = 10.24PDD1060 pKa = 3.67GSALVPPRR1068 pKa = 11.84VVFQPASPYY1077 pKa = 9.82RR1078 pKa = 11.84ASDD1081 pKa = 3.54HH1082 pKa = 7.33DD1083 pKa = 4.15PVLVGLFAVSDD1094 pKa = 3.99LAVTLTDD1101 pKa = 3.45SPDD1104 pKa = 3.43PVVAGNNLTYY1114 pKa = 10.54TITVSNNGPDD1124 pKa = 3.49AATAASWSLSLPPGRR1139 pKa = 11.84SFVSLPAVAGWTCTTPAVGSGGTVSCSNPGFSVGSGVFTLTAAVDD1184 pKa = 3.47PSIAAGTVLTATATVTSASGDD1205 pKa = 3.58PTPGNNSATATTTVGTSADD1224 pKa = 3.49LGVTNSAAPATAISGQAITYY1244 pKa = 7.71TITASNAGPSDD1255 pKa = 3.46AAGVSLSNPLPANTTFASLAAPGGWSCTTPAVGATGTVSCTAASLAVTSAVFTLVVTVDD1314 pKa = 3.47AGTTAANITDD1324 pKa = 3.84TATVSSATSDD1334 pKa = 3.6PNPGNEE1340 pKa = 4.29SATATTPLSAEE1351 pKa = 4.02ADD1353 pKa = 3.55LSVTHH1358 pKa = 6.23VATPLSGPDD1367 pKa = 3.53LAPGEE1372 pKa = 4.46SIQYY1376 pKa = 11.24AMTVTNVGPSSAALVTLSNIMPPQTTLNSLTAPPGWSCTTPALGSNGTISCTRR1429 pKa = 11.84PTLAPGSSTITFVVLLDD1446 pKa = 3.66AGYY1449 pKa = 10.52LGSTVVDD1456 pKa = 3.82TASVTSATTDD1466 pKa = 3.57PAPGNEE1472 pKa = 4.28TQTATTPVLASADD1485 pKa = 3.67LAVSNTASTPTAINGQPITYY1505 pKa = 8.62TVTVTNNGPSNAATVAMNNPIPANTTFTAATTPPGWNCTLPAAGGTGTVACANASLAPGSAVFSITVTVNPGAPVTMIVDD1585 pKa = 4.35TATVSSATGDD1595 pKa = 3.51PATGNEE1601 pKa = 4.37SATATTSTPVSLQSFEE1617 pKa = 4.01VDD1619 pKa = 2.88

Molecular weight: 162.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V3DN13|A0A4V3DN13_9GAMM WD40 repeat protein OS=Tahibacter aquaticus OX=520092 GN=DFR29_103212 PE=4 SV=1
MM1 pKa = 7.58LMLMLGFLLWPLPLPLPLPLPLPLRR26 pKa = 11.84LRR28 pKa = 11.84LRR30 pKa = 11.84LRR32 pKa = 11.84LRR34 pKa = 11.84LLVLVLVLVLVILLWLVILLWLLPLPLLLRR64 pKa = 11.84AGSAPLPGPHH74 pKa = 7.49RR75 pKa = 11.84SRR77 pKa = 4.27

Molecular weight: 8.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5664

0

5664

2081458

27

5836

367.5

39.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.49 ± 0.055

0.991 ± 0.011

5.67 ± 0.026

4.96 ± 0.032

3.401 ± 0.019

8.35 ± 0.038

2.087 ± 0.018

4.039 ± 0.021

2.519 ± 0.029

11.051 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.774 ± 0.016

2.818 ± 0.028

5.27 ± 0.029

4.019 ± 0.023

7.521 ± 0.04

5.913 ± 0.031

5.067 ± 0.044

7.118 ± 0.026

1.521 ± 0.015

2.422 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski