Amino acid dipepetide frequency for Synechococcus phage S-IOM18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.719AlaAla: 6.719 ± 0.558
0.453AlaCys: 0.453 ± 0.098
4.605AlaAsp: 4.605 ± 0.303
3.888AlaGlu: 3.888 ± 0.414
3.001AlaPhe: 3.001 ± 0.278
6.719AlaGly: 6.719 ± 0.593
0.887AlaHis: 0.887 ± 0.122
4.228AlaIle: 4.228 ± 0.294
3.888AlaLys: 3.888 ± 0.446
4.775AlaLeu: 4.775 ± 0.418
1.453AlaMet: 1.453 ± 0.209
4.02AlaAsn: 4.02 ± 0.417
2.737AlaPro: 2.737 ± 0.271
2.737AlaGln: 2.737 ± 0.222
2.68AlaArg: 2.68 ± 0.251
5.398AlaSer: 5.398 ± 0.46
6.096AlaThr: 6.096 ± 0.739
4.662AlaVal: 4.662 ± 0.312
0.604AlaTrp: 0.604 ± 0.113
2.171AlaTyr: 2.171 ± 0.193
0.0AlaXaa: 0.0 ± 0.0
Cys
0.547CysAla: 0.547 ± 0.104
0.094CysCys: 0.094 ± 0.042
0.642CysAsp: 0.642 ± 0.126
0.453CysGlu: 0.453 ± 0.094
0.415CysPhe: 0.415 ± 0.099
0.679CysGly: 0.679 ± 0.121
0.208CysHis: 0.208 ± 0.067
0.396CysIle: 0.396 ± 0.099
0.623CysLys: 0.623 ± 0.126
0.661CysLeu: 0.661 ± 0.123
0.283CysMet: 0.283 ± 0.088
0.51CysAsn: 0.51 ± 0.131
0.396CysPro: 0.396 ± 0.104
0.359CysGln: 0.359 ± 0.079
0.283CysArg: 0.283 ± 0.097
0.623CysSer: 0.623 ± 0.115
0.623CysThr: 0.623 ± 0.13
0.491CysVal: 0.491 ± 0.097
0.075CysTrp: 0.075 ± 0.034
0.34CysTyr: 0.34 ± 0.076
0.0CysXaa: 0.0 ± 0.0
Asp
5.228AspAla: 5.228 ± 0.359
0.623AspCys: 0.623 ± 0.135
4.775AspAsp: 4.775 ± 0.327
4.19AspGlu: 4.19 ± 0.332
3.058AspPhe: 3.058 ± 0.278
5.719AspGly: 5.719 ± 0.379
1.038AspHis: 1.038 ± 0.165
4.133AspIle: 4.133 ± 0.323
3.341AspLys: 3.341 ± 0.373
4.02AspLeu: 4.02 ± 0.305
1.567AspMet: 1.567 ± 0.176
3.907AspAsn: 3.907 ± 0.308
3.058AspPro: 3.058 ± 0.244
1.774AspGln: 1.774 ± 0.183
2.491AspArg: 2.491 ± 0.273
4.341AspSer: 4.341 ± 0.396
4.737AspThr: 4.737 ± 0.449
4.133AspVal: 4.133 ± 0.269
1.019AspTrp: 1.019 ± 0.151
3.209AspTyr: 3.209 ± 0.254
0.0AspXaa: 0.0 ± 0.0
Glu
3.511GluAla: 3.511 ± 0.317
0.887GluCys: 0.887 ± 0.139
3.982GluAsp: 3.982 ± 0.325
5.36GluGlu: 5.36 ± 0.663
2.907GluPhe: 2.907 ± 0.223
4.096GluGly: 4.096 ± 0.328
1.246GluHis: 1.246 ± 0.179
4.473GluIle: 4.473 ± 0.347
3.548GluLys: 3.548 ± 0.524
4.983GluLeu: 4.983 ± 0.356
1.472GluMet: 1.472 ± 0.181
2.888GluAsn: 2.888 ± 0.222
2.001GluPro: 2.001 ± 0.179
2.699GluGln: 2.699 ± 0.287
2.661GluArg: 2.661 ± 0.31
3.85GluSer: 3.85 ± 0.394
4.039GluThr: 4.039 ± 0.265
4.718GluVal: 4.718 ± 0.298
0.793GluTrp: 0.793 ± 0.142
2.888GluTyr: 2.888 ± 0.254
0.0GluXaa: 0.0 ± 0.0
Phe
2.831PheAla: 2.831 ± 0.238
0.491PheCys: 0.491 ± 0.113
3.624PheAsp: 3.624 ± 0.246
2.548PheGlu: 2.548 ± 0.206
1.925PhePhe: 1.925 ± 0.183
3.133PheGly: 3.133 ± 0.233
0.585PheHis: 0.585 ± 0.123
2.586PheIle: 2.586 ± 0.273
2.378PheLys: 2.378 ± 0.232
2.793PheLeu: 2.793 ± 0.276
1.114PheMet: 1.114 ± 0.147
2.699PheAsn: 2.699 ± 0.23
1.434PhePro: 1.434 ± 0.196
1.869PheGln: 1.869 ± 0.154
1.85PheArg: 1.85 ± 0.184
2.869PheSer: 2.869 ± 0.224
3.322PheThr: 3.322 ± 0.413
2.737PheVal: 2.737 ± 0.284
0.359PheTrp: 0.359 ± 0.076
1.642PheTyr: 1.642 ± 0.169
0.0PheXaa: 0.0 ± 0.0
Gly
6.323GlyAla: 6.323 ± 0.632
0.604GlyCys: 0.604 ± 0.121
5.341GlyAsp: 5.341 ± 0.407
4.756GlyGlu: 4.756 ± 0.256
3.114GlyPhe: 3.114 ± 0.255
7.776GlyGly: 7.776 ± 0.677
1.095GlyHis: 1.095 ± 0.155
4.171GlyIle: 4.171 ± 0.374
3.982GlyLys: 3.982 ± 0.402
4.983GlyLeu: 4.983 ± 0.384
1.585GlyMet: 1.585 ± 0.26
4.284GlyAsn: 4.284 ± 0.368
2.057GlyPro: 2.057 ± 0.237
2.944GlyGln: 2.944 ± 0.333
3.001GlyArg: 3.001 ± 0.278
6.53GlySer: 6.53 ± 0.537
7.682GlyThr: 7.682 ± 0.853
5.153GlyVal: 5.153 ± 0.444
1.095GlyTrp: 1.095 ± 0.158
3.662GlyTyr: 3.662 ± 0.316
0.0GlyXaa: 0.0 ± 0.0
His
0.661HisAla: 0.661 ± 0.121
0.264HisCys: 0.264 ± 0.071
1.038HisAsp: 1.038 ± 0.156
0.963HisGlu: 0.963 ± 0.157
0.944HisPhe: 0.944 ± 0.153
0.981HisGly: 0.981 ± 0.147
0.377HisHis: 0.377 ± 0.097
0.83HisIle: 0.83 ± 0.111
0.849HisLys: 0.849 ± 0.153
1.0HisLeu: 1.0 ± 0.173
0.434HisMet: 0.434 ± 0.093
0.755HisAsn: 0.755 ± 0.126
0.868HisPro: 0.868 ± 0.187
0.453HisGln: 0.453 ± 0.106
0.717HisArg: 0.717 ± 0.137
0.774HisSer: 0.774 ± 0.138
1.114HisThr: 1.114 ± 0.144
0.812HisVal: 0.812 ± 0.143
0.245HisTrp: 0.245 ± 0.067
0.755HisTyr: 0.755 ± 0.105
0.0HisXaa: 0.0 ± 0.0
Ile
3.831IleAla: 3.831 ± 0.302
0.528IleCys: 0.528 ± 0.11
4.643IleAsp: 4.643 ± 0.356
4.096IleGlu: 4.096 ± 0.285
2.567IlePhe: 2.567 ± 0.219
4.266IleGly: 4.266 ± 0.329
0.736IleHis: 0.736 ± 0.124
3.303IleIle: 3.303 ± 0.244
3.699IleLys: 3.699 ± 0.314
3.926IleLeu: 3.926 ± 0.292
0.925IleMet: 0.925 ± 0.18
4.001IleAsn: 4.001 ± 0.265
2.756IlePro: 2.756 ± 0.318
2.265IleGln: 2.265 ± 0.216
2.529IleArg: 2.529 ± 0.247
4.209IleSer: 4.209 ± 0.432
5.738IleThr: 5.738 ± 0.643
3.813IleVal: 3.813 ± 0.399
0.566IleTrp: 0.566 ± 0.109
1.925IleTyr: 1.925 ± 0.211
0.0IleXaa: 0.0 ± 0.0
Lys
3.529LysAla: 3.529 ± 0.441
0.642LysCys: 0.642 ± 0.134
3.378LysAsp: 3.378 ± 0.311
3.907LysGlu: 3.907 ± 0.581
2.359LysPhe: 2.359 ± 0.241
3.416LysGly: 3.416 ± 0.386
0.963LysHis: 0.963 ± 0.187
3.586LysIle: 3.586 ± 0.314
4.53LysLys: 4.53 ± 0.806
4.454LysLeu: 4.454 ± 0.425
1.472LysMet: 1.472 ± 0.267
2.737LysAsn: 2.737 ± 0.261
1.869LysPro: 1.869 ± 0.294
2.152LysGln: 2.152 ± 0.308
2.491LysArg: 2.491 ± 0.354
3.511LysSer: 3.511 ± 0.374
3.718LysThr: 3.718 ± 0.268
3.926LysVal: 3.926 ± 0.279
0.755LysTrp: 0.755 ± 0.13
2.699LysTyr: 2.699 ± 0.349
0.0LysXaa: 0.0 ± 0.0
Leu
4.586LeuAla: 4.586 ± 0.333
0.661LeuCys: 0.661 ± 0.156
5.171LeuAsp: 5.171 ± 0.358
4.303LeuGlu: 4.303 ± 0.374
2.51LeuPhe: 2.51 ± 0.23
4.945LeuGly: 4.945 ± 0.533
1.17LeuHis: 1.17 ± 0.165
3.869LeuIle: 3.869 ± 0.287
4.473LeuLys: 4.473 ± 0.468
5.53LeuLeu: 5.53 ± 0.378
1.567LeuMet: 1.567 ± 0.274
4.454LeuAsn: 4.454 ± 0.365
2.812LeuPro: 2.812 ± 0.269
3.133LeuGln: 3.133 ± 0.24
3.152LeuArg: 3.152 ± 0.276
5.077LeuSer: 5.077 ± 0.319
6.115LeuThr: 6.115 ± 0.541
4.586LeuVal: 4.586 ± 0.256
0.415LeuTrp: 0.415 ± 0.105
3.058LeuTyr: 3.058 ± 0.288
0.0LeuXaa: 0.0 ± 0.0
Met
1.68MetAla: 1.68 ± 0.294
0.189MetCys: 0.189 ± 0.061
1.038MetAsp: 1.038 ± 0.191
1.491MetGlu: 1.491 ± 0.231
0.887MetPhe: 0.887 ± 0.149
1.321MetGly: 1.321 ± 0.255
0.415MetHis: 0.415 ± 0.101
1.114MetIle: 1.114 ± 0.198
1.491MetLys: 1.491 ± 0.268
1.755MetLeu: 1.755 ± 0.199
0.547MetMet: 0.547 ± 0.122
1.283MetAsn: 1.283 ± 0.181
0.906MetPro: 0.906 ± 0.151
1.019MetGln: 1.019 ± 0.195
1.038MetArg: 1.038 ± 0.16
1.755MetSer: 1.755 ± 0.258
1.718MetThr: 1.718 ± 0.19
1.038MetVal: 1.038 ± 0.147
0.245MetTrp: 0.245 ± 0.065
0.736MetTyr: 0.736 ± 0.145
0.0MetXaa: 0.0 ± 0.0
Asn
4.039AsnAla: 4.039 ± 0.362
0.415AsnCys: 0.415 ± 0.089
3.227AsnAsp: 3.227 ± 0.211
2.831AsnGlu: 2.831 ± 0.219
2.246AsnPhe: 2.246 ± 0.194
4.907AsnGly: 4.907 ± 0.453
0.812AsnHis: 0.812 ± 0.118
3.831AsnIle: 3.831 ± 0.426
2.831AsnLys: 2.831 ± 0.284
4.549AsnLeu: 4.549 ± 0.375
1.0AsnMet: 1.0 ± 0.134
3.133AsnAsn: 3.133 ± 0.282
3.02AsnPro: 3.02 ± 0.216
2.02AsnGln: 2.02 ± 0.221
2.114AsnArg: 2.114 ± 0.16
3.888AsnSer: 3.888 ± 0.287
3.982AsnThr: 3.982 ± 0.404
4.284AsnVal: 4.284 ± 0.296
0.642AsnTrp: 0.642 ± 0.123
2.623AsnTyr: 2.623 ± 0.193
0.0AsnXaa: 0.0 ± 0.0
Pro
2.718ProAla: 2.718 ± 0.256
0.302ProCys: 0.302 ± 0.085
2.529ProAsp: 2.529 ± 0.289
2.944ProGlu: 2.944 ± 0.273
1.661ProPhe: 1.661 ± 0.169
3.303ProGly: 3.303 ± 0.28
0.698ProHis: 0.698 ± 0.109
2.284ProIle: 2.284 ± 0.239
1.85ProLys: 1.85 ± 0.276
2.152ProLeu: 2.152 ± 0.222
0.717ProMet: 0.717 ± 0.183
2.303ProAsn: 2.303 ± 0.248
1.85ProPro: 1.85 ± 0.235
1.17ProGln: 1.17 ± 0.167
1.585ProArg: 1.585 ± 0.15
2.925ProSer: 2.925 ± 0.226
3.152ProThr: 3.152 ± 0.289
2.491ProVal: 2.491 ± 0.228
0.528ProTrp: 0.528 ± 0.103
1.793ProTyr: 1.793 ± 0.196
0.0ProXaa: 0.0 ± 0.0
Gln
2.227GlnAla: 2.227 ± 0.286
0.283GlnCys: 0.283 ± 0.1
1.982GlnAsp: 1.982 ± 0.178
2.869GlnGlu: 2.869 ± 0.26
1.906GlnPhe: 1.906 ± 0.228
2.623GlnGly: 2.623 ± 0.287
0.774GlnHis: 0.774 ± 0.142
2.416GlnIle: 2.416 ± 0.238
2.416GlnLys: 2.416 ± 0.307
3.171GlnLeu: 3.171 ± 0.274
1.019GlnMet: 1.019 ± 0.187
1.793GlnAsn: 1.793 ± 0.168
1.283GlnPro: 1.283 ± 0.166
1.925GlnGln: 1.925 ± 0.204
1.661GlnArg: 1.661 ± 0.178
1.982GlnSer: 1.982 ± 0.197
2.359GlnThr: 2.359 ± 0.24
2.623GlnVal: 2.623 ± 0.22
0.585GlnTrp: 0.585 ± 0.11
1.812GlnTyr: 1.812 ± 0.148
0.0GlnXaa: 0.0 ± 0.0
Arg
3.001ArgAla: 3.001 ± 0.297
0.226ArgCys: 0.226 ± 0.048
2.246ArgAsp: 2.246 ± 0.168
2.133ArgGlu: 2.133 ± 0.262
1.944ArgPhe: 1.944 ± 0.175
2.869ArgGly: 2.869 ± 0.265
0.679ArgHis: 0.679 ± 0.124
2.567ArgIle: 2.567 ± 0.225
2.397ArgLys: 2.397 ± 0.318
3.378ArgLeu: 3.378 ± 0.238
1.132ArgMet: 1.132 ± 0.205
2.095ArgAsn: 2.095 ± 0.173
1.453ArgPro: 1.453 ± 0.187
1.623ArgGln: 1.623 ± 0.18
2.171ArgArg: 2.171 ± 0.382
2.321ArgSer: 2.321 ± 0.228
2.567ArgThr: 2.567 ± 0.272
3.152ArgVal: 3.152 ± 0.279
0.528ArgTrp: 0.528 ± 0.085
2.057ArgTyr: 2.057 ± 0.179
0.0ArgXaa: 0.0 ± 0.0
Ser
5.417SerAla: 5.417 ± 0.428
0.491SerCys: 0.491 ± 0.112
4.266SerAsp: 4.266 ± 0.286
3.68SerGlu: 3.68 ± 0.25
3.265SerPhe: 3.265 ± 0.338
7.304SerGly: 7.304 ± 0.634
0.623SerHis: 0.623 ± 0.091
4.568SerIle: 4.568 ± 0.547
3.397SerLys: 3.397 ± 0.406
5.058SerLeu: 5.058 ± 0.278
1.491SerMet: 1.491 ± 0.25
3.831SerAsn: 3.831 ± 0.288
2.303SerPro: 2.303 ± 0.254
2.246SerGln: 2.246 ± 0.202
2.208SerArg: 2.208 ± 0.207
5.889SerSer: 5.889 ± 0.734
5.473SerThr: 5.473 ± 0.529
4.775SerVal: 4.775 ± 0.383
0.736SerTrp: 0.736 ± 0.11
2.812SerTyr: 2.812 ± 0.238
0.0SerXaa: 0.0 ± 0.0
Thr
6.946ThrAla: 6.946 ± 0.807
0.434ThrCys: 0.434 ± 0.106
4.624ThrAsp: 4.624 ± 0.406
4.322ThrGlu: 4.322 ± 0.376
3.322ThrPhe: 3.322 ± 0.361
7.531ThrGly: 7.531 ± 0.912
0.774ThrHis: 0.774 ± 0.127
4.926ThrIle: 4.926 ± 0.527
3.416ThrLys: 3.416 ± 0.257
6.417ThrLeu: 6.417 ± 0.666
1.246ThrMet: 1.246 ± 0.153
4.133ThrAsn: 4.133 ± 0.476
3.152ThrPro: 3.152 ± 0.286
2.812ThrGln: 2.812 ± 0.296
2.793ThrArg: 2.793 ± 0.313
5.606ThrSer: 5.606 ± 0.597
6.304ThrThr: 6.304 ± 0.728
6.153ThrVal: 6.153 ± 0.74
0.661ThrTrp: 0.661 ± 0.114
2.661ThrTyr: 2.661 ± 0.177
0.0ThrXaa: 0.0 ± 0.0
Val
4.662ValAla: 4.662 ± 0.321
0.472ValCys: 0.472 ± 0.099
5.285ValAsp: 5.285 ± 0.476
4.813ValGlu: 4.813 ± 0.326
2.586ValPhe: 2.586 ± 0.249
5.19ValGly: 5.19 ± 0.475
0.83ValHis: 0.83 ± 0.122
3.85ValIle: 3.85 ± 0.331
3.624ValLys: 3.624 ± 0.25
4.247ValLeu: 4.247 ± 0.263
1.359ValMet: 1.359 ± 0.202
4.152ValAsn: 4.152 ± 0.305
3.114ValPro: 3.114 ± 0.25
2.529ValGln: 2.529 ± 0.18
2.529ValArg: 2.529 ± 0.206
4.983ValSer: 4.983 ± 0.291
5.738ValThr: 5.738 ± 0.609
4.775ValVal: 4.775 ± 0.376
0.547ValTrp: 0.547 ± 0.09
2.812ValTyr: 2.812 ± 0.356
0.0ValXaa: 0.0 ± 0.0
Trp
0.83TrpAla: 0.83 ± 0.103
0.17TrpCys: 0.17 ± 0.057
0.642TrpAsp: 0.642 ± 0.098
0.698TrpGlu: 0.698 ± 0.135
0.491TrpPhe: 0.491 ± 0.097
0.755TrpGly: 0.755 ± 0.108
0.359TrpHis: 0.359 ± 0.066
0.491TrpIle: 0.491 ± 0.079
0.868TrpLys: 0.868 ± 0.142
0.679TrpLeu: 0.679 ± 0.138
0.321TrpMet: 0.321 ± 0.094
0.793TrpAsn: 0.793 ± 0.132
0.208TrpPro: 0.208 ± 0.06
0.377TrpGln: 0.377 ± 0.09
0.51TrpArg: 0.51 ± 0.095
0.868TrpSer: 0.868 ± 0.148
0.642TrpThr: 0.642 ± 0.115
0.793TrpVal: 0.793 ± 0.156
0.113TrpTrp: 0.113 ± 0.043
0.377TrpTyr: 0.377 ± 0.079
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.435TyrAla: 2.435 ± 0.206
0.472TyrCys: 0.472 ± 0.104
3.322TyrAsp: 3.322 ± 0.273
2.756TyrGlu: 2.756 ± 0.301
1.68TyrPhe: 1.68 ± 0.225
2.623TyrGly: 2.623 ± 0.251
0.566TyrHis: 0.566 ± 0.097
2.699TyrIle: 2.699 ± 0.244
2.491TyrLys: 2.491 ± 0.237
3.058TyrLeu: 3.058 ± 0.241
0.944TyrMet: 0.944 ± 0.18
2.623TyrAsn: 2.623 ± 0.202
1.68TyrPro: 1.68 ± 0.193
1.604TyrGln: 1.604 ± 0.198
2.076TyrArg: 2.076 ± 0.2
2.435TyrSer: 2.435 ± 0.286
3.114TyrThr: 3.114 ± 0.303
2.982TyrVal: 2.982 ± 0.287
0.472TyrTrp: 0.472 ± 0.114
2.001TyrTyr: 2.001 ± 0.196
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 209 proteins (52984 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski