Cassava mosaic Madagascar virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H8WR47|H8WR47_9GEMI Capsid protein OS=Cassava mosaic Madagascar virus OX=1125764 GN=AV1 PE=3 SV=1
MM1 pKa = 7.6LVQQVVLHH9 pKa = 5.97FKK11 pKa = 9.35MWDD14 pKa = 3.27PLLNEE19 pKa = 4.99FPDD22 pKa = 3.86SVHH25 pKa = 6.81GFRR28 pKa = 11.84CMLAIKK34 pKa = 9.52YY35 pKa = 8.25LQAVEE40 pKa = 4.08VTYY43 pKa = 10.81EE44 pKa = 4.19SNTLGRR50 pKa = 11.84DD51 pKa = 4.16LIRR54 pKa = 11.84DD55 pKa = 4.62LISVIRR61 pKa = 11.84ARR63 pKa = 11.84DD64 pKa = 3.41YY65 pKa = 10.78VEE67 pKa = 3.75ASRR70 pKa = 11.84RR71 pKa = 11.84YY72 pKa = 8.54NNCHH76 pKa = 5.86SRR78 pKa = 11.84LEE80 pKa = 4.42GASEE84 pKa = 4.19AEE86 pKa = 4.07LRR88 pKa = 11.84QPIQQPCCCPHH99 pKa = 6.72CPRR102 pKa = 11.84HH103 pKa = 5.56KK104 pKa = 9.81QVQIMEE110 pKa = 4.52VQTHH114 pKa = 4.83VSEE117 pKa = 4.54AQDD120 pKa = 3.4VQDD123 pKa = 3.6VQKK126 pKa = 10.8PP127 pKa = 3.39

Molecular weight: 14.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H8WR48|H8WR48_9GEMI Replication enhancer (Fragment) OS=Cassava mosaic Madagascar virus OX=1125764 GN=AC3 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.75IIIATPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84KK20 pKa = 10.58LNFDD24 pKa = 3.32SPYY27 pKa = 9.93SSRR30 pKa = 11.84AVVPIVPGTSKK41 pKa = 10.48FKK43 pKa = 9.84SWKK46 pKa = 8.54FRR48 pKa = 11.84PMYY51 pKa = 9.78RR52 pKa = 11.84KK53 pKa = 9.15PRR55 pKa = 11.84MYY57 pKa = 11.01RR58 pKa = 11.84MFKK61 pKa = 10.72SPDD64 pKa = 3.23VPRR67 pKa = 11.84GCEE70 pKa = 4.34GPCKK74 pKa = 9.32VQSYY78 pKa = 6.99EE79 pKa = 3.67QRR81 pKa = 11.84DD82 pKa = 4.07DD83 pKa = 3.72VKK85 pKa = 10.14HH86 pKa = 5.93TGTVRR91 pKa = 11.84CVSDD95 pKa = 3.28VTRR98 pKa = 11.84GPGITHH104 pKa = 6.76RR105 pKa = 11.84VGKK108 pKa = 9.68RR109 pKa = 11.84FCIKK113 pKa = 10.26SIYY116 pKa = 9.86VLGKK120 pKa = 9.13IWMDD124 pKa = 3.49EE125 pKa = 4.09NIKK128 pKa = 10.06KK129 pKa = 9.97QNHH132 pKa = 4.95TNQVMFFLVRR142 pKa = 11.84DD143 pKa = 3.69RR144 pKa = 11.84RR145 pKa = 11.84PYY147 pKa = 8.79GTSPMDD153 pKa = 3.74FGQVFNMFDD162 pKa = 3.99NEE164 pKa = 4.11PSTATVKK171 pKa = 10.81NDD173 pKa = 2.84LRR175 pKa = 11.84DD176 pKa = 3.43RR177 pKa = 11.84YY178 pKa = 9.52QVMRR182 pKa = 11.84KK183 pKa = 7.66FHH185 pKa = 5.81ATVVGGPSGMKK196 pKa = 8.93EE197 pKa = 3.94QLWLGDD203 pKa = 3.74FLGLIIMWCIITRR216 pKa = 11.84RR217 pKa = 11.84QLSMRR222 pKa = 11.84IIQRR226 pKa = 11.84MRR228 pKa = 11.84YY229 pKa = 7.7CCIWHH234 pKa = 5.9VRR236 pKa = 11.84MPLIQCMLRR245 pKa = 11.84LKK247 pKa = 9.94YY248 pKa = 10.88ASIFMMQQ255 pKa = 2.96

Molecular weight: 29.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1697

85

359

212.1

24.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.302 ± 0.69

2.239 ± 0.355

5.186 ± 0.285

4.302 ± 0.562

4.243 ± 0.317

4.714 ± 0.721

3.83 ± 0.814

6.423 ± 0.566

5.893 ± 0.656

7.248 ± 0.454

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.887 ± 0.599

4.596 ± 0.593

6.541 ± 0.52

4.42 ± 0.704

6.718 ± 0.931

8.662 ± 0.801

6.423 ± 0.688

6.07 ± 1.088

1.355 ± 0.191

3.948 ± 0.507
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski