Liparis tanakae (Tanaka s snailfish)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei;

Average proteome isoelectric point is 7.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68164 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Z2EB35|A0A4Z2EB35_9TELE D-amino-acid oxidase OS=Liparis tanakae OX=230148 GN=DAO_0 PE=3 SV=1
MM1 pKa = 8.03LEE3 pKa = 4.24DD4 pKa = 4.0GATKK8 pKa = 10.33RR9 pKa = 11.84HH10 pKa = 5.95RR11 pKa = 11.84KK12 pKa = 9.17APPHH16 pKa = 6.31SLTPCRR22 pKa = 11.84GQRR25 pKa = 11.84DD26 pKa = 3.85RR27 pKa = 11.84GTEE30 pKa = 3.95GQRR33 pKa = 11.84DD34 pKa = 3.79RR35 pKa = 11.84GTEE38 pKa = 3.95GQRR41 pKa = 11.84VRR43 pKa = 11.84GTEE46 pKa = 3.85GQRR49 pKa = 11.84DD50 pKa = 3.7YY51 pKa = 12.03DD52 pKa = 4.91DD53 pKa = 5.78IEE55 pKa = 4.07QQHH58 pKa = 6.32YY59 pKa = 11.26DD60 pKa = 3.75DD61 pKa = 5.79IEE63 pKa = 4.08QQGYY67 pKa = 11.0DD68 pKa = 3.92DD69 pKa = 5.41IEE71 pKa = 4.22KK72 pKa = 10.48QSYY75 pKa = 11.03DD76 pKa = 3.63DD77 pKa = 5.38IEE79 pKa = 4.29RR80 pKa = 11.84QSYY83 pKa = 11.39DD84 pKa = 4.01DD85 pKa = 5.0IKK87 pKa = 9.99QQHH90 pKa = 6.26YY91 pKa = 11.27DD92 pKa = 3.71DD93 pKa = 5.59IEE95 pKa = 4.03QQSYY99 pKa = 11.7DD100 pKa = 4.19DD101 pKa = 5.4IEE103 pKa = 4.16QQSYY107 pKa = 11.7DD108 pKa = 4.19DD109 pKa = 5.4IEE111 pKa = 4.16QQSYY115 pKa = 11.72DD116 pKa = 3.75DD117 pKa = 5.12IEE119 pKa = 4.27RR120 pKa = 11.84QSYY123 pKa = 11.4DD124 pKa = 3.74DD125 pKa = 5.07IKK127 pKa = 10.22QQSYY131 pKa = 11.7DD132 pKa = 3.97DD133 pKa = 4.91IEE135 pKa = 4.16QQSYY139 pKa = 11.7DD140 pKa = 4.19DD141 pKa = 5.4IEE143 pKa = 4.16QQSYY147 pKa = 11.77DD148 pKa = 4.35DD149 pKa = 4.97IKK151 pKa = 9.98QQHH154 pKa = 6.26YY155 pKa = 11.29DD156 pKa = 3.64DD157 pKa = 5.56IEE159 pKa = 4.08QQGYY163 pKa = 11.0DD164 pKa = 3.92DD165 pKa = 5.41IEE167 pKa = 4.22KK168 pKa = 10.48QSYY171 pKa = 11.09DD172 pKa = 3.9DD173 pKa = 5.11IEE175 pKa = 4.28KK176 pKa = 10.53QSYY179 pKa = 11.02DD180 pKa = 3.51DD181 pKa = 4.48IEE183 pKa = 4.16RR184 pKa = 11.84HH185 pKa = 6.19SYY187 pKa = 11.55DD188 pKa = 4.84DD189 pKa = 4.49IKK191 pKa = 10.35QQSYY195 pKa = 11.7DD196 pKa = 3.97DD197 pKa = 4.91IEE199 pKa = 4.16QQSYY203 pKa = 11.7DD204 pKa = 4.19DD205 pKa = 5.4IEE207 pKa = 4.16QQSYY211 pKa = 11.7DD212 pKa = 4.19DD213 pKa = 5.4IEE215 pKa = 4.16QQSYY219 pKa = 11.7DD220 pKa = 4.19DD221 pKa = 5.4IEE223 pKa = 4.16QQSYY227 pKa = 11.77DD228 pKa = 4.35DD229 pKa = 4.97IKK231 pKa = 9.98QQHH234 pKa = 6.26YY235 pKa = 11.27DD236 pKa = 3.71DD237 pKa = 5.59IEE239 pKa = 4.03QQSYY243 pKa = 11.72DD244 pKa = 3.61DD245 pKa = 4.71IEE247 pKa = 4.09RR248 pKa = 11.84QRR250 pKa = 11.84YY251 pKa = 9.48DD252 pKa = 4.74DD253 pKa = 5.46IEE255 pKa = 4.11QQSYY259 pKa = 11.7DD260 pKa = 4.19DD261 pKa = 5.4IEE263 pKa = 4.16QQSYY267 pKa = 11.6DD268 pKa = 4.3DD269 pKa = 3.9IRR271 pKa = 11.84VMMSS275 pKa = 2.91

Molecular weight: 33.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Z2EVT5|A0A4Z2EVT5_9TELE Uncharacterized protein OS=Liparis tanakae OX=230148 GN=EYF80_056908 PE=4 SV=1
MM1 pKa = 6.97KK2 pKa = 9.47TLRR5 pKa = 11.84PFSPQVLPLFQNPRR19 pKa = 11.84CAARR23 pKa = 11.84RR24 pKa = 11.84PALQSLSVLQTGGFRR39 pKa = 11.84HH40 pKa = 5.4TRR42 pKa = 11.84GSRR45 pKa = 11.84RR46 pKa = 11.84GSRR49 pKa = 11.84LAAHH53 pKa = 7.83ASRR56 pKa = 11.84LTPRR60 pKa = 11.84GSRR63 pKa = 11.84LAAHH67 pKa = 5.21XTHH70 pKa = 7.21RR71 pKa = 11.84GSRR74 pKa = 11.84TAAHH78 pKa = 7.31APRR81 pKa = 11.84LTPRR85 pKa = 11.84GSRR88 pKa = 11.84TAAHH92 pKa = 7.55ASRR95 pKa = 11.84LTHH98 pKa = 6.34RR99 pKa = 11.84GSRR102 pKa = 11.84LAAHH106 pKa = 7.8ASRR109 pKa = 11.84LTHH112 pKa = 6.32RR113 pKa = 11.84GSRR116 pKa = 11.84LAPHH120 pKa = 7.68ASRR123 pKa = 11.84LTHH126 pKa = 6.59RR127 pKa = 11.84GSSSVRR133 pKa = 11.84RR134 pKa = 11.84NVASS138 pKa = 3.38

Molecular weight: 14.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68148

16

68164

15190440

8

13673

222.9

24.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.486 ± 0.014

2.221 ± 0.008

4.709 ± 0.011

6.728 ± 0.018

3.144 ± 0.009

7.46 ± 0.014

2.839 ± 0.008

3.44 ± 0.013

4.759 ± 0.017

9.413 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.009

3.057 ± 0.009

6.303 ± 0.016

4.404 ± 0.011

7.427 ± 0.014

8.928 ± 0.014

5.471 ± 0.011

6.454 ± 0.011

1.277 ± 0.005

2.04 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski