Tepidimonas charontis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Tepidimonas

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2630 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A554XF65|A0A554XF65_9BURK Murein hydrolase activator NlpD OS=Tepidimonas charontis OX=2267262 GN=nlpD PE=4 SV=1
MM1 pKa = 6.84TAVAEE6 pKa = 4.57TTAEE10 pKa = 4.16ATTEE14 pKa = 4.04MPAPLIFTDD23 pKa = 3.73AAAAKK28 pKa = 9.24VAEE31 pKa = 5.03LIAEE35 pKa = 4.31EE36 pKa = 4.69GNPEE40 pKa = 3.75LKK42 pKa = 10.68LRR44 pKa = 11.84VFVQGGGCSGFQYY57 pKa = 10.9GFTFDD62 pKa = 4.42EE63 pKa = 4.73VVNDD67 pKa = 5.59DD68 pKa = 3.92DD69 pKa = 4.47TQMTKK74 pKa = 10.88NGVTLLIDD82 pKa = 3.64AMSYY86 pKa = 10.32QYY88 pKa = 11.5LVGAEE93 pKa = 3.79IDD95 pKa = 3.91YY96 pKa = 11.36KK97 pKa = 11.19EE98 pKa = 4.67DD99 pKa = 3.23LQGAQFVIRR108 pKa = 11.84NPNATTTCGCGSSFSVGG125 pKa = 3.1

Molecular weight: 13.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A554XC67|A0A554XC67_9BURK DNA topoisomerase OS=Tepidimonas charontis OX=2267262 GN=topB PE=3 SV=1
MM1 pKa = 6.96STVTSGQRR9 pKa = 11.84ASGRR13 pKa = 11.84TGPPSVPVTSNSMPWMWMGWLVIVKK38 pKa = 10.05LPMRR42 pKa = 11.84TRR44 pKa = 11.84TRR46 pKa = 11.84SPRR49 pKa = 11.84RR50 pKa = 11.84ATNGSMPGKK59 pKa = 8.6TRR61 pKa = 11.84LFHH64 pKa = 6.79AQMLKK69 pKa = 10.6SSMVLGLGVRR79 pKa = 11.84APGVTSKK86 pKa = 11.22ALSRR90 pKa = 11.84KK91 pKa = 6.97QQ92 pKa = 3.14

Molecular weight: 10.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2630

0

2630

830006

29

2501

315.6

34.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.122 ± 0.084

0.959 ± 0.013

5.265 ± 0.031

5.324 ± 0.043

3.041 ± 0.029

7.982 ± 0.042

2.493 ± 0.025

4.029 ± 0.037

2.475 ± 0.037

10.949 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.085 ± 0.02

2.061 ± 0.027

5.711 ± 0.041

4.214 ± 0.032

8.274 ± 0.052

4.283 ± 0.028

4.996 ± 0.032

7.786 ± 0.037

1.823 ± 0.032

2.129 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski