bacterium HR27

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2050 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5YWH9|A0A2H5YWH9_9BACT Uncharacterized protein OS=bacterium HR27 OX=2035422 GN=HRbin27_00277 PE=4 SV=1
MM1 pKa = 7.73AGEE4 pKa = 4.34TEE6 pKa = 4.28TWDD9 pKa = 3.27GTPEE13 pKa = 4.57HH14 pKa = 7.4EE15 pKa = 4.72DD16 pKa = 3.54LTWLTEE22 pKa = 3.92PPLYY26 pKa = 10.11WIVIHH31 pKa = 6.35SEE33 pKa = 4.11PLPPIPGLVYY43 pKa = 10.71LATNSFADD51 pKa = 3.34SAYY54 pKa = 11.14NEE56 pKa = 4.37TLVAAPDD63 pKa = 3.69EE64 pKa = 4.68PEE66 pKa = 5.25LIEE69 pKa = 4.88LLRR72 pKa = 11.84LAAGVRR78 pKa = 11.84AVVPGYY84 pKa = 10.54LRR86 pKa = 11.84YY87 pKa = 9.69FFQDD91 pKa = 3.25DD92 pKa = 3.38AVIAVVNGTAITWFDD107 pKa = 3.77SVDD110 pKa = 3.19EE111 pKa = 4.18ALRR114 pKa = 11.84WDD116 pKa = 4.43PFAEE120 pKa = 5.16PDD122 pKa = 3.46TMLPPGPTSS131 pKa = 3.05

Molecular weight: 14.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5Z026|A0A2H5Z026_9BACT Uncharacterized protein OS=bacterium HR27 OX=2035422 GN=HRbin27_01539 PE=4 SV=1
MM1 pKa = 7.63TSPALPPLGTRR12 pKa = 11.84PNPASSAAVNARR24 pKa = 11.84VARR27 pKa = 11.84GASVKK32 pKa = 10.01GPWMVPPTRR41 pKa = 11.84WFRR44 pKa = 11.84KK45 pKa = 9.61RR46 pKa = 11.84ISIGNASRR54 pKa = 11.84FWTTTVLQSGPKK66 pKa = 9.51PPGNVSPLL74 pKa = 3.38

Molecular weight: 7.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2050

0

2050

573660

29

1595

279.8

30.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.77 ± 0.069

0.916 ± 0.02

5.067 ± 0.044

6.811 ± 0.064

3.171 ± 0.034

7.888 ± 0.052

2.148 ± 0.03

4.462 ± 0.044

1.521 ± 0.034

11.597 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.597 ± 0.02

1.702 ± 0.028

6.189 ± 0.046

3.551 ± 0.039

9.324 ± 0.067

4.419 ± 0.038

5.268 ± 0.043

8.758 ± 0.054

1.623 ± 0.031

2.217 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski