Soybean dwarf virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Luteovirus

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q91QR2|Q91QR2_9LUTE Movement protein OS=Soybean dwarf virus OX=12049 GN=ORF4 PE=3 SV=1
VV1 pKa = 7.46DD2 pKa = 4.87GEE4 pKa = 4.6PGPKK8 pKa = 9.42PGPDD12 pKa = 3.72PAPQPTPTPEE22 pKa = 3.71PTPAKK27 pKa = 9.75HH28 pKa = 6.09EE29 pKa = 4.09RR30 pKa = 11.84FIAYY34 pKa = 7.69TGTLSTLISARR45 pKa = 11.84QSSDD49 pKa = 3.46SISLYY54 pKa = 10.01SIRR57 pKa = 11.84SQRR60 pKa = 11.84IRR62 pKa = 11.84YY63 pKa = 9.1IEE65 pKa = 4.77DD66 pKa = 3.59EE67 pKa = 4.13NSSWTNIDD75 pKa = 3.62AKK77 pKa = 10.16WYY79 pKa = 8.63SQNSVEE85 pKa = 4.73AIPMFVYY92 pKa = 9.48PVPEE96 pKa = 4.3GTWSIEE102 pKa = 3.88ISCEE106 pKa = 3.95GYY108 pKa = 9.81QAASSTSDD116 pKa = 3.17PHH118 pKa = 7.88RR119 pKa = 11.84GKK121 pKa = 10.64CDD123 pKa = 2.94GMIAYY128 pKa = 9.88DD129 pKa = 4.3DD130 pKa = 4.93DD131 pKa = 4.88SSKK134 pKa = 10.55VWNVGQQNNVTITNNKK150 pKa = 9.17ADD152 pKa = 4.07NDD154 pKa = 4.0WKK156 pKa = 11.0YY157 pKa = 11.15GHH159 pKa = 7.75PDD161 pKa = 3.01LTINGDD167 pKa = 3.54RR168 pKa = 11.84FDD170 pKa = 3.77QNQVVEE176 pKa = 4.13KK177 pKa = 10.71DD178 pKa = 3.98GIISFHH184 pKa = 6.69LVTTGPNASFFLVAPAVKK202 pKa = 8.86KK203 pKa = 6.9TAKK206 pKa = 10.58YY207 pKa = 9.35NFCVSYY213 pKa = 11.32GDD215 pKa = 3.19WTDD218 pKa = 3.56RR219 pKa = 11.84DD220 pKa = 3.87MEE222 pKa = 4.49FGMVSVVLDD231 pKa = 3.47EE232 pKa = 4.66HH233 pKa = 8.3LEE235 pKa = 4.22GARR238 pKa = 11.84SSQYY242 pKa = 9.71VRR244 pKa = 11.84KK245 pKa = 7.62TPRR248 pKa = 11.84SGHH251 pKa = 5.3VGVNRR256 pKa = 11.84SHH258 pKa = 7.25RR259 pKa = 11.84LQDD262 pKa = 3.19NFVPTEE268 pKa = 3.97YY269 pKa = 11.14VSDD272 pKa = 3.89EE273 pKa = 4.39DD274 pKa = 4.06SSSNSSIVSNRR285 pKa = 11.84PSTPDD290 pKa = 3.17NDD292 pKa = 3.69SDD294 pKa = 4.09AKK296 pKa = 10.53FAEE299 pKa = 4.34SMKK302 pKa = 10.83GKK304 pKa = 10.29LPSQTKK310 pKa = 10.17LPPKK314 pKa = 10.43GFLSQLSTKK323 pKa = 8.39EE324 pKa = 3.74KK325 pKa = 11.0KK326 pKa = 10.13EE327 pKa = 3.72ISNSKK332 pKa = 8.98PSNVEE337 pKa = 3.79GLVGPLVAAYY347 pKa = 8.65GYY349 pKa = 9.02PSQTGVHH356 pKa = 6.36DD357 pKa = 4.11AARR360 pKa = 11.84EE361 pKa = 3.71ILQAKK366 pKa = 8.14EE367 pKa = 3.51AAEE370 pKa = 3.85NLAEE374 pKa = 4.64LEE376 pKa = 4.14RR377 pKa = 11.84DD378 pKa = 3.2LKK380 pKa = 10.8EE381 pKa = 4.13INKK384 pKa = 9.88LEE386 pKa = 4.37PPDD389 pKa = 4.53VIVQEE394 pKa = 4.66EE395 pKa = 4.43IPDD398 pKa = 3.87FVPPSEE404 pKa = 5.26KK405 pKa = 9.61ILKK408 pKa = 9.96EE409 pKa = 4.11DD410 pKa = 4.1DD411 pKa = 3.75PDD413 pKa = 3.72YY414 pKa = 11.5VPPIWHH420 pKa = 6.66NADD423 pKa = 3.08QAVLVSSYY431 pKa = 10.73EE432 pKa = 3.88PPDD435 pKa = 3.33WSRR438 pKa = 11.84PAYY441 pKa = 10.0EE442 pKa = 5.22SGDD445 pKa = 3.8PPKK448 pKa = 10.29KK449 pKa = 9.24TGTLKK454 pKa = 10.1GTLSKK459 pKa = 11.03LGGSLRR465 pKa = 11.84SGEE468 pKa = 4.1SSLRR472 pKa = 11.84GSLRR476 pKa = 11.84KK477 pKa = 7.81TQDD480 pKa = 2.88QTDD483 pKa = 4.12LDD485 pKa = 4.08NKK487 pKa = 10.25LSKK490 pKa = 10.77LSVIQRR496 pKa = 11.84SRR498 pKa = 11.84YY499 pKa = 8.05QRR501 pKa = 11.84ILNNLGKK508 pKa = 9.24MRR510 pKa = 11.84ARR512 pKa = 11.84TYY514 pKa = 10.81IDD516 pKa = 4.79GLDD519 pKa = 3.54LDD521 pKa = 4.29

Molecular weight:
57.7 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q91QR4|Q91QR4_9LUTE RNA-directed RNA polymerase (Fragment) OS=Soybean dwarf virus OX=12049 GN=ORF2 PE=4 SV=1
MM1 pKa = 6.96VAVSNVAIQRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84TRR15 pKa = 11.84RR16 pKa = 11.84AARR19 pKa = 11.84RR20 pKa = 11.84APRR23 pKa = 11.84VQLMAVPTVTSRR35 pKa = 11.84PQRR38 pKa = 11.84RR39 pKa = 11.84GRR41 pKa = 11.84QRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84NNRR50 pKa = 11.84GGSFISGGSGKK61 pKa = 10.11AHH63 pKa = 5.8TFVFSKK69 pKa = 10.92DD70 pKa = 3.62GINGSSKK77 pKa = 10.83GSITFGPSLSEE88 pKa = 4.21CKK90 pKa = 10.0PFSDD94 pKa = 5.84GILKK98 pKa = 10.28AYY100 pKa = 10.09HH101 pKa = 6.59EE102 pKa = 4.33YY103 pKa = 10.69KK104 pKa = 9.21ITSILLQFITEE115 pKa = 4.23ASSTSSGSIAYY126 pKa = 9.43EE127 pKa = 3.79LDD129 pKa = 3.32PHH131 pKa = 6.86CKK133 pKa = 9.64YY134 pKa = 11.08SEE136 pKa = 4.03IQSLLNKK143 pKa = 10.04FSITKK148 pKa = 9.95SGSKK152 pKa = 10.08RR153 pKa = 11.84FPTRR157 pKa = 11.84AINGLEE163 pKa = 3.76WHH165 pKa = 6.97DD166 pKa = 3.68TSEE169 pKa = 4.34DD170 pKa = 3.42QFKK173 pKa = 10.28IHH175 pKa = 6.26YY176 pKa = 8.83KK177 pKa = 10.89GNGEE181 pKa = 4.19SKK183 pKa = 10.11IAGSFKK189 pKa = 10.52ISINVLTQNAKK200 pKa = 10.25

Molecular weight:
22.23 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1806

189

535

361.2

40.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.423 ± 0.797

1.218 ± 0.327

5.814 ± 0.883

6.257 ± 0.449

3.931 ± 0.644

5.592 ± 0.774

2.547 ± 0.28

5.482 ± 0.485

6.921 ± 1.036

7.198 ± 0.492

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.547 ± 0.619

4.817 ± 0.104

5.759 ± 0.841

3.599 ± 0.32

6.478 ± 0.788

10.299 ± 1.488

4.319 ± 0.619

6.368 ± 0.494

1.107 ± 0.173

3.322 ± 0.127

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski