Trypanosoma cruzi (strain CL Brener)

Taxonomy: cellular organisms; Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Trypanosoma; Schizotrypanum; Trypanosoma cruzi

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19243 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4DH69|Q4DH69_TRYCC Uncharacterized protein OS=Trypanosoma cruzi (strain CL Brener) OX=353153 GN=Tc00.1047053509527.140 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 5.65GYY4 pKa = 11.61SLLEE8 pKa = 3.98RR9 pKa = 11.84LDD11 pKa = 4.05AFFSEE16 pKa = 5.14GEE18 pKa = 4.06NTDD21 pKa = 3.77AIGNFLSEE29 pKa = 4.44EE30 pKa = 4.01QGVMQLLGQPTDD42 pKa = 3.42SQEE45 pKa = 4.03ALEE48 pKa = 4.99FYY50 pKa = 11.15SLFKK54 pKa = 10.55RR55 pKa = 11.84YY56 pKa = 9.75AVVVDD61 pKa = 3.6NLLNAFIEE69 pKa = 4.49RR70 pKa = 11.84EE71 pKa = 4.21SKK73 pKa = 10.38LGYY76 pKa = 10.72VIDD79 pKa = 4.64LEE81 pKa = 4.22QLAAAVMNEE90 pKa = 3.34WHH92 pKa = 6.29QEE94 pKa = 3.62QDD96 pKa = 2.83FCRR99 pKa = 11.84YY100 pKa = 9.26VCTAYY105 pKa = 9.86IAGALDD111 pKa = 3.59FDD113 pKa = 4.48SFKK116 pKa = 11.22QLVADD121 pKa = 4.12VNAITAYY128 pKa = 9.92PFGDD132 pKa = 3.72EE133 pKa = 4.9SSDD136 pKa = 3.36ADD138 pKa = 4.37SVTEE142 pKa = 4.0TNTQEE147 pKa = 4.08EE148 pKa = 5.06EE149 pKa = 3.89II150 pKa = 4.62

Molecular weight: 16.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4D504|Q4D504_TRYCC Ras-related protein rab-5 putative (Fragment) OS=Trypanosoma cruzi (strain CL Brener) OX=353153 GN=Tc00.1047053511269.4 PE=4 SV=1
RR1 pKa = 7.48RR2 pKa = 11.84RR3 pKa = 11.84LRR5 pKa = 11.84SAWRR9 pKa = 11.84RR10 pKa = 11.84SWRR13 pKa = 11.84CARR16 pKa = 11.84TTSKK20 pKa = 10.71SVQTTSKK27 pKa = 10.85SVLLLPRR34 pKa = 11.84MPLAVAVPLQGRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84LRR51 pKa = 11.84SAWRR55 pKa = 11.84RR56 pKa = 11.84SWRR59 pKa = 11.84NARR62 pKa = 11.84TTSKK66 pKa = 10.85SVLLLPRR73 pKa = 11.84MPLAVAVPLQGRR85 pKa = 11.84RR86 pKa = 11.84RR87 pKa = 11.84RR88 pKa = 11.84LRR90 pKa = 11.84SAWRR94 pKa = 11.84RR95 pKa = 11.84SWRR98 pKa = 11.84CARR101 pKa = 11.84TTSKK105 pKa = 10.65SVQTISKK112 pKa = 10.23SVLLLPRR119 pKa = 11.84MPLAVAVPLQGRR131 pKa = 11.84RR132 pKa = 11.84RR133 pKa = 11.84RR134 pKa = 11.84LRR136 pKa = 11.84SAWRR140 pKa = 11.84RR141 pKa = 11.84SWRR144 pKa = 11.84NARR147 pKa = 11.84TTSKK151 pKa = 10.85SVLLLPRR158 pKa = 11.84MPLAVAVPLQGRR170 pKa = 11.84RR171 pKa = 11.84RR172 pKa = 11.84RR173 pKa = 11.84LRR175 pKa = 11.84SAWRR179 pKa = 11.84RR180 pKa = 11.84SWRR183 pKa = 11.84NARR186 pKa = 11.84TTSKK190 pKa = 10.85SVLLLPRR197 pKa = 11.84MPLAVAVPLQGRR209 pKa = 11.84RR210 pKa = 11.84RR211 pKa = 11.84RR212 pKa = 11.84LRR214 pKa = 11.84SAWRR218 pKa = 11.84RR219 pKa = 11.84SWRR222 pKa = 11.84NARR225 pKa = 11.84TTSKK229 pKa = 10.85SVLLLPRR236 pKa = 11.84MPLAVAVPLQGRR248 pKa = 11.84RR249 pKa = 11.84RR250 pKa = 11.84RR251 pKa = 11.84LRR253 pKa = 11.84SAWRR257 pKa = 11.84RR258 pKa = 11.84SWRR261 pKa = 11.84CARR264 pKa = 11.84TTSKK268 pKa = 10.71SVQTTSKK275 pKa = 10.85SVLLLPRR282 pKa = 11.84MPLAVAVPLQGRR294 pKa = 11.84RR295 pKa = 11.84RR296 pKa = 11.84RR297 pKa = 11.84LRR299 pKa = 11.84SAWRR303 pKa = 11.84RR304 pKa = 11.84SWRR307 pKa = 11.84NARR310 pKa = 11.84TTSKK314 pKa = 10.85SVLLLPRR321 pKa = 11.84MPLAVAVPLQGRR333 pKa = 11.84RR334 pKa = 11.84RR335 pKa = 11.84RR336 pKa = 11.84LRR338 pKa = 11.84SAWRR342 pKa = 11.84RR343 pKa = 11.84SWRR346 pKa = 11.84CARR349 pKa = 11.84TTSKK353 pKa = 10.65SVQTISKK360 pKa = 10.23SVLLLPRR367 pKa = 11.84MPLAVAVPLQGRR379 pKa = 11.84RR380 pKa = 11.84RR381 pKa = 11.84RR382 pKa = 11.84LRR384 pKa = 11.84SAWRR388 pKa = 11.84RR389 pKa = 11.84SWRR392 pKa = 11.84NARR395 pKa = 11.84TTSKK399 pKa = 10.85SVLLLPRR406 pKa = 11.84MPLAVAVPLQGRR418 pKa = 11.84RR419 pKa = 11.84RR420 pKa = 11.84RR421 pKa = 11.84LRR423 pKa = 11.84SAWRR427 pKa = 11.84RR428 pKa = 11.84SWRR431 pKa = 11.84NARR434 pKa = 11.84TTSKK438 pKa = 10.85SVLLLPRR445 pKa = 11.84MPLAVAVPLQGRR457 pKa = 11.84RR458 pKa = 11.84RR459 pKa = 11.84RR460 pKa = 11.84LRR462 pKa = 11.84SAWRR466 pKa = 11.84RR467 pKa = 11.84SWRR470 pKa = 11.84CARR473 pKa = 11.84TTSKK477 pKa = 10.71SVQTTSKK484 pKa = 10.85SVLLLPRR491 pKa = 11.84MPLAVAVPLQGRR503 pKa = 11.84RR504 pKa = 11.84RR505 pKa = 11.84RR506 pKa = 11.84LRR508 pKa = 11.84SAWRR512 pKa = 11.84RR513 pKa = 11.84SWRR516 pKa = 11.84CARR519 pKa = 11.84TTSKK523 pKa = 10.71SVQTTSKK530 pKa = 10.85SVLLLPRR537 pKa = 11.84MPFAVAVPLQGRR549 pKa = 11.84RR550 pKa = 11.84RR551 pKa = 11.84RR552 pKa = 11.84LRR554 pKa = 11.84SAWRR558 pKa = 11.84RR559 pKa = 11.84SWRR562 pKa = 11.84CARR565 pKa = 11.84TTSKK569 pKa = 10.71SVQTTSKK576 pKa = 10.85SVLLLPRR583 pKa = 11.84MPLAVAVPLQGRR595 pKa = 11.84RR596 pKa = 11.84RR597 pKa = 11.84RR598 pKa = 11.84LRR600 pKa = 11.84SAWKK604 pKa = 10.23RR605 pKa = 11.84SWRR608 pKa = 11.84CARR611 pKa = 11.84TTSKK615 pKa = 10.88SVLLLPRR622 pKa = 11.84MPLAVAVPLQGRR634 pKa = 11.84RR635 pKa = 11.84RR636 pKa = 11.84RR637 pKa = 11.84LRR639 pKa = 11.84SAWRR643 pKa = 11.84RR644 pKa = 11.84SWRR647 pKa = 11.84CARR650 pKa = 11.84TTSKK654 pKa = 10.88SVLLLPRR661 pKa = 11.84MPLAVAVPLQGRR673 pKa = 11.84RR674 pKa = 11.84RR675 pKa = 11.84RR676 pKa = 11.84LRR678 pKa = 11.84SAWRR682 pKa = 11.84RR683 pKa = 11.84SWRR686 pKa = 11.84CARR689 pKa = 11.84TTSKK693 pKa = 10.88SVLLLPRR700 pKa = 11.84MPLAVAVPLQGRR712 pKa = 11.84RR713 pKa = 11.84RR714 pKa = 11.84RR715 pKa = 11.84LRR717 pKa = 11.84SAWRR721 pKa = 11.84RR722 pKa = 11.84SWRR725 pKa = 11.84CARR728 pKa = 11.84TTSKK732 pKa = 10.88SVLLLPRR739 pKa = 11.84MPLAVAVPLQGRR751 pKa = 11.84RR752 pKa = 11.84RR753 pKa = 11.84RR754 pKa = 11.84LRR756 pKa = 11.84SAWRR760 pKa = 11.84RR761 pKa = 11.84SWRR764 pKa = 11.84CARR767 pKa = 11.84TTSKK771 pKa = 10.88SVLLLPRR778 pKa = 11.84MPLAVAVPLQGRR790 pKa = 11.84RR791 pKa = 11.84RR792 pKa = 11.84RR793 pKa = 11.84LRR795 pKa = 11.84SAWRR799 pKa = 11.84RR800 pKa = 11.84SWRR803 pKa = 11.84NARR806 pKa = 11.84TTSKK810 pKa = 10.85SVLLLPRR817 pKa = 11.84MPLAVAVPLQGRR829 pKa = 11.84RR830 pKa = 11.84RR831 pKa = 11.84RR832 pKa = 11.84LRR834 pKa = 11.84SAWRR838 pKa = 11.84RR839 pKa = 11.84SWRR842 pKa = 11.84CARR845 pKa = 11.84TISKK849 pKa = 10.31SVLLLPRR856 pKa = 11.84MPLAVAVPLQGRR868 pKa = 11.84RR869 pKa = 11.84RR870 pKa = 11.84RR871 pKa = 11.84LRR873 pKa = 11.84SAWRR877 pKa = 11.84RR878 pKa = 11.84SWRR881 pKa = 11.84NARR884 pKa = 11.84TTSKK888 pKa = 10.85SVLLLPRR895 pKa = 11.84MPLAVAVPLQGRR907 pKa = 11.84RR908 pKa = 11.84RR909 pKa = 11.84RR910 pKa = 11.84LRR912 pKa = 11.84SAWRR916 pKa = 11.84RR917 pKa = 11.84SWRR920 pKa = 11.84NARR923 pKa = 11.84TTSKK927 pKa = 10.85SVLLLPRR934 pKa = 11.84MPLAVAVPLQGRR946 pKa = 11.84RR947 pKa = 11.84RR948 pKa = 11.84RR949 pKa = 11.84LRR951 pKa = 11.84SAWRR955 pKa = 11.84RR956 pKa = 11.84SWRR959 pKa = 11.84NARR962 pKa = 11.84TTSKK966 pKa = 10.85SVLLLPRR973 pKa = 11.84MPLAVAVPLQGRR985 pKa = 11.84RR986 pKa = 11.84RR987 pKa = 11.84RR988 pKa = 11.84LRR990 pKa = 11.84SAWKK994 pKa = 10.23RR995 pKa = 11.84SWRR998 pKa = 11.84CARR1001 pKa = 11.84TTSKK1005 pKa = 10.71SVQTTSKK1012 pKa = 10.87SVLLPSMMPLQGRR1025 pKa = 11.84RR1026 pKa = 11.84RR1027 pKa = 11.84RR1028 pKa = 11.84LRR1030 pKa = 11.84SAWRR1034 pKa = 11.84RR1035 pKa = 11.84SWRR1038 pKa = 11.84NARR1041 pKa = 11.84TTSRR1045 pKa = 11.84IMWHH1049 pKa = 5.86RR1050 pKa = 11.84WW1051 pKa = 2.87

Molecular weight: 123.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19242

1

19243

9671180

18

4848

502.6

55.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.812 ± 0.023

2.121 ± 0.01

4.858 ± 0.01

6.958 ± 0.028

3.63 ± 0.013

7.002 ± 0.025

2.444 ± 0.008

3.651 ± 0.014

4.263 ± 0.021

9.533 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.007

3.552 ± 0.009

4.948 ± 0.015

3.627 ± 0.014

6.805 ± 0.019

8.12 ± 0.021

5.981 ± 0.016

7.718 ± 0.029

1.178 ± 0.006

2.364 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski