Amino acid dipepetide frequency for Enterococcus italicus (strain DSM 15952 / CCUG 50447 / LMG 22039 / TP 1.5)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.81AlaAla: 5.81 ± 0.13
0.607AlaCys: 0.607 ± 0.031
3.985AlaAsp: 3.985 ± 0.081
4.702AlaGlu: 4.702 ± 0.106
3.393AlaPhe: 3.393 ± 0.077
4.799AlaGly: 4.799 ± 0.097
1.34AlaHis: 1.34 ± 0.044
6.548AlaIle: 6.548 ± 0.118
5.38AlaLys: 5.38 ± 0.1
7.321AlaLeu: 7.321 ± 0.105
2.186AlaMet: 2.186 ± 0.059
3.383AlaAsn: 3.383 ± 0.075
2.031AlaPro: 2.031 ± 0.063
3.117AlaGln: 3.117 ± 0.081
2.702AlaArg: 2.702 ± 0.072
4.468AlaSer: 4.468 ± 0.091
4.724AlaThr: 4.724 ± 0.102
5.312AlaVal: 5.312 ± 0.096
0.605AlaTrp: 0.605 ± 0.031
2.687AlaTyr: 2.687 ± 0.071
0.0AlaXaa: 0.0 ± 0.0
Cys
0.421CysAla: 0.421 ± 0.025
0.096CysCys: 0.096 ± 0.013
0.334CysAsp: 0.334 ± 0.022
0.4CysGlu: 0.4 ± 0.028
0.359CysPhe: 0.359 ± 0.026
0.627CysGly: 0.627 ± 0.034
0.195CysHis: 0.195 ± 0.019
0.411CysIle: 0.411 ± 0.026
0.258CysLys: 0.258 ± 0.019
0.713CysLeu: 0.713 ± 0.034
0.144CysMet: 0.144 ± 0.014
0.21CysAsn: 0.21 ± 0.019
0.29CysPro: 0.29 ± 0.024
0.327CysGln: 0.327 ± 0.027
0.233CysArg: 0.233 ± 0.018
0.461CysSer: 0.461 ± 0.026
0.328CysThr: 0.328 ± 0.022
0.401CysVal: 0.401 ± 0.026
0.058CysTrp: 0.058 ± 0.009
0.315CysTyr: 0.315 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
3.884AspAla: 3.884 ± 0.085
0.397AspCys: 0.397 ± 0.027
2.569AspAsp: 2.569 ± 0.087
4.239AspGlu: 4.239 ± 0.082
2.622AspPhe: 2.622 ± 0.066
3.531AspGly: 3.531 ± 0.077
1.048AspHis: 1.048 ± 0.037
3.664AspIle: 3.664 ± 0.075
2.937AspLys: 2.937 ± 0.077
5.006AspLeu: 5.006 ± 0.099
1.26AspMet: 1.26 ± 0.048
1.904AspAsn: 1.904 ± 0.051
1.818AspPro: 1.818 ± 0.056
2.323AspGln: 2.323 ± 0.06
2.052AspArg: 2.052 ± 0.057
2.869AspSer: 2.869 ± 0.067
3.005AspThr: 3.005 ± 0.08
3.725AspVal: 3.725 ± 0.083
0.65AspTrp: 0.65 ± 0.034
2.394AspTyr: 2.394 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
5.309GluAla: 5.309 ± 0.108
0.363GluCys: 0.363 ± 0.025
3.313GluAsp: 3.313 ± 0.077
6.06GluGlu: 6.06 ± 0.119
2.537GluPhe: 2.537 ± 0.064
3.712GluGly: 3.712 ± 0.089
1.255GluHis: 1.255 ± 0.052
4.785GluIle: 4.785 ± 0.08
6.054GluLys: 6.054 ± 0.106
7.269GluLeu: 7.269 ± 0.127
2.14GluMet: 2.14 ± 0.055
3.301GluAsn: 3.301 ± 0.075
1.834GluPro: 1.834 ± 0.075
3.842GluGln: 3.842 ± 0.088
3.142GluArg: 3.142 ± 0.081
3.447GluSer: 3.447 ± 0.092
3.8GluThr: 3.8 ± 0.08
4.653GluVal: 4.653 ± 0.087
0.714GluTrp: 0.714 ± 0.032
1.992GluTyr: 1.992 ± 0.05
0.0GluXaa: 0.0 ± 0.0
Phe
3.173PheAla: 3.173 ± 0.07
0.338PheCys: 0.338 ± 0.021
2.779PheAsp: 2.779 ± 0.067
2.782PheGlu: 2.782 ± 0.074
2.706PhePhe: 2.706 ± 0.084
3.305PheGly: 3.305 ± 0.078
0.981PheHis: 0.981 ± 0.04
3.279PheIle: 3.279 ± 0.081
2.221PheLys: 2.221 ± 0.067
4.87PheLeu: 4.87 ± 0.107
1.053PheMet: 1.053 ± 0.038
1.662PheAsn: 1.662 ± 0.051
1.64PhePro: 1.64 ± 0.046
1.954PheGln: 1.954 ± 0.058
1.687PheArg: 1.687 ± 0.052
3.351PheSer: 3.351 ± 0.08
2.473PheThr: 2.473 ± 0.052
3.368PheVal: 3.368 ± 0.068
0.43PheTrp: 0.43 ± 0.03
1.863PheTyr: 1.863 ± 0.054
0.0PheXaa: 0.0 ± 0.0
Gly
4.603GlyAla: 4.603 ± 0.106
0.515GlyCys: 0.515 ± 0.03
3.047GlyAsp: 3.047 ± 0.082
4.051GlyGlu: 4.051 ± 0.073
3.075GlyPhe: 3.075 ± 0.065
4.312GlyGly: 4.312 ± 0.108
1.285GlyHis: 1.285 ± 0.048
5.614GlyIle: 5.614 ± 0.083
4.705GlyLys: 4.705 ± 0.084
6.176GlyLeu: 6.176 ± 0.111
1.965GlyMet: 1.965 ± 0.054
2.556GlyAsn: 2.556 ± 0.068
1.443GlyPro: 1.443 ± 0.051
2.438GlyGln: 2.438 ± 0.064
2.479GlyArg: 2.479 ± 0.062
3.709GlySer: 3.709 ± 0.082
4.126GlyThr: 4.126 ± 0.076
4.836GlyVal: 4.836 ± 0.102
0.617GlyTrp: 0.617 ± 0.033
2.734GlyTyr: 2.734 ± 0.07
0.0GlyXaa: 0.0 ± 0.0
His
1.371HisAla: 1.371 ± 0.044
0.185HisCys: 0.185 ± 0.018
0.932HisAsp: 0.932 ± 0.037
1.257HisGlu: 1.257 ± 0.046
1.073HisPhe: 1.073 ± 0.035
1.225HisGly: 1.225 ± 0.044
0.601HisHis: 0.601 ± 0.03
1.255HisIle: 1.255 ± 0.047
0.817HisLys: 0.817 ± 0.031
2.164HisLeu: 2.164 ± 0.06
0.453HisMet: 0.453 ± 0.025
0.617HisAsn: 0.617 ± 0.031
1.096HisPro: 1.096 ± 0.035
1.073HisGln: 1.073 ± 0.04
0.862HisArg: 0.862 ± 0.035
1.165HisSer: 1.165 ± 0.04
1.121HisThr: 1.121 ± 0.045
1.285HisVal: 1.285 ± 0.043
0.23HisTrp: 0.23 ± 0.015
0.922HisTyr: 0.922 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
6.031IleAla: 6.031 ± 0.109
0.554IleCys: 0.554 ± 0.029
4.437IleAsp: 4.437 ± 0.089
5.023IleGlu: 5.023 ± 0.096
3.219IlePhe: 3.219 ± 0.079
5.587IleGly: 5.587 ± 0.112
1.581IleHis: 1.581 ± 0.05
5.132IleIle: 5.132 ± 0.114
4.053IleLys: 4.053 ± 0.072
6.707IleLeu: 6.707 ± 0.115
1.642IleMet: 1.642 ± 0.052
2.861IleAsn: 2.861 ± 0.072
2.972IlePro: 2.972 ± 0.067
3.422IleGln: 3.422 ± 0.068
3.11IleArg: 3.11 ± 0.074
4.657IleSer: 4.657 ± 0.089
4.045IleThr: 4.045 ± 0.084
5.663IleVal: 5.663 ± 0.091
0.592IleTrp: 0.592 ± 0.03
2.436IleTyr: 2.436 ± 0.056
0.0IleXaa: 0.0 ± 0.0
Lys
4.612LysAla: 4.612 ± 0.086
0.245LysCys: 0.245 ± 0.02
3.526LysAsp: 3.526 ± 0.067
6.682LysGlu: 6.682 ± 0.103
1.83LysPhe: 1.83 ± 0.052
3.839LysGly: 3.839 ± 0.067
1.078LysHis: 1.078 ± 0.043
4.753LysIle: 4.753 ± 0.084
6.398LysLys: 6.398 ± 0.118
5.433LysLeu: 5.433 ± 0.11
2.225LysMet: 2.225 ± 0.061
3.606LysAsn: 3.606 ± 0.081
1.897LysPro: 1.897 ± 0.058
3.677LysGln: 3.677 ± 0.069
3.222LysArg: 3.222 ± 0.083
3.327LysSer: 3.327 ± 0.065
3.992LysThr: 3.992 ± 0.081
4.405LysVal: 4.405 ± 0.095
0.678LysTrp: 0.678 ± 0.035
1.987LysTyr: 1.987 ± 0.061
0.0LysXaa: 0.0 ± 0.0
Leu
9.107LeuAla: 9.107 ± 0.127
0.65LeuCys: 0.65 ± 0.036
5.016LeuAsp: 5.016 ± 0.097
5.816LeuGlu: 5.816 ± 0.107
4.984LeuPhe: 4.984 ± 0.108
6.089LeuGly: 6.089 ± 0.097
1.814LeuHis: 1.814 ± 0.053
6.706LeuIle: 6.706 ± 0.125
6.254LeuLys: 6.254 ± 0.098
10.243LeuLeu: 10.243 ± 0.159
2.447LeuMet: 2.447 ± 0.066
4.103LeuAsn: 4.103 ± 0.077
4.25LeuPro: 4.25 ± 0.074
3.88LeuGln: 3.88 ± 0.074
3.59LeuArg: 3.59 ± 0.077
6.838LeuSer: 6.838 ± 0.096
6.593LeuThr: 6.593 ± 0.099
6.905LeuVal: 6.905 ± 0.097
0.752LeuTrp: 0.752 ± 0.034
3.09LeuTyr: 3.09 ± 0.079
0.0LeuXaa: 0.0 ± 0.0
Met
2.108MetAla: 2.108 ± 0.058
0.141MetCys: 0.141 ± 0.014
1.42MetAsp: 1.42 ± 0.043
1.554MetGlu: 1.554 ± 0.049
0.92MetPhe: 0.92 ± 0.041
1.687MetGly: 1.687 ± 0.048
0.456MetHis: 0.456 ± 0.022
2.289MetIle: 2.289 ± 0.059
2.295MetLys: 2.295 ± 0.06
2.159MetLeu: 2.159 ± 0.067
0.849MetMet: 0.849 ± 0.043
1.62MetAsn: 1.62 ± 0.056
0.86MetPro: 0.86 ± 0.034
1.047MetGln: 1.047 ± 0.045
1.078MetArg: 1.078 ± 0.042
1.595MetSer: 1.595 ± 0.047
1.83MetThr: 1.83 ± 0.048
1.817MetVal: 1.817 ± 0.052
0.152MetTrp: 0.152 ± 0.013
0.84MetTyr: 0.84 ± 0.037
0.0MetXaa: 0.0 ± 0.0
Asn
2.703AsnAla: 2.703 ± 0.067
0.287AsnCys: 0.287 ± 0.022
2.241AsnAsp: 2.241 ± 0.064
3.295AsnGlu: 3.295 ± 0.066
1.763AsnPhe: 1.763 ± 0.058
3.187AsnGly: 3.187 ± 0.073
1.145AsnHis: 1.145 ± 0.048
2.74AsnIle: 2.74 ± 0.068
2.617AsnLys: 2.617 ± 0.066
3.811AsnLeu: 3.811 ± 0.082
1.069AsnMet: 1.069 ± 0.037
1.842AsnAsn: 1.842 ± 0.057
1.947AsnPro: 1.947 ± 0.06
2.821AsnGln: 2.821 ± 0.064
2.062AsnArg: 2.062 ± 0.055
2.226AsnSer: 2.226 ± 0.065
2.116AsnThr: 2.116 ± 0.063
2.721AsnVal: 2.721 ± 0.072
0.499AsnTrp: 0.499 ± 0.027
1.844AsnTyr: 1.844 ± 0.055
0.0AsnXaa: 0.0 ± 0.0
Pro
2.269ProAla: 2.269 ± 0.067
0.171ProCys: 0.171 ± 0.014
2.018ProAsp: 2.018 ± 0.059
2.762ProGlu: 2.762 ± 0.074
1.828ProPhe: 1.828 ± 0.052
1.923ProGly: 1.923 ± 0.062
0.669ProHis: 0.669 ± 0.03
2.603ProIle: 2.603 ± 0.066
2.342ProLys: 2.342 ± 0.067
3.292ProLeu: 3.292 ± 0.076
0.824ProMet: 0.824 ± 0.036
1.629ProAsn: 1.629 ± 0.05
0.607ProPro: 0.607 ± 0.028
1.213ProGln: 1.213 ± 0.039
1.061ProArg: 1.061 ± 0.039
2.1ProSer: 2.1 ± 0.056
2.426ProThr: 2.426 ± 0.084
2.566ProVal: 2.566 ± 0.06
0.295ProTrp: 0.295 ± 0.021
1.359ProTyr: 1.359 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
4.297GlnAla: 4.297 ± 0.09
0.2GlnCys: 0.2 ± 0.02
1.691GlnAsp: 1.691 ± 0.051
3.335GlnGlu: 3.335 ± 0.076
1.878GlnPhe: 1.878 ± 0.052
2.346GlnGly: 2.346 ± 0.059
0.767GlnHis: 0.767 ± 0.035
2.868GlnIle: 2.868 ± 0.067
3.451GlnLys: 3.451 ± 0.08
5.589GlnLeu: 5.589 ± 0.118
1.315GlnMet: 1.315 ± 0.05
1.684GlnAsn: 1.684 ± 0.049
1.511GlnPro: 1.511 ± 0.047
2.505GlnGln: 2.505 ± 0.085
1.929GlnArg: 1.929 ± 0.057
2.674GlnSer: 2.674 ± 0.058
3.011GlnThr: 3.011 ± 0.064
3.179GlnVal: 3.179 ± 0.063
0.512GlnTrp: 0.512 ± 0.025
1.445GlnTyr: 1.445 ± 0.047
0.0GlnXaa: 0.0 ± 0.0
Arg
2.52ArgAla: 2.52 ± 0.061
0.261ArgCys: 0.261 ± 0.02
1.869ArgAsp: 1.869 ± 0.061
2.798ArgGlu: 2.798 ± 0.072
2.019ArgPhe: 2.019 ± 0.06
2.18ArgGly: 2.18 ± 0.062
0.789ArgHis: 0.789 ± 0.031
2.888ArgIle: 2.888 ± 0.066
3.192ArgLys: 3.192 ± 0.072
4.077ArgLeu: 4.077 ± 0.086
1.296ArgMet: 1.296 ± 0.047
1.776ArgAsn: 1.776 ± 0.054
1.287ArgPro: 1.287 ± 0.046
2.105ArgGln: 2.105 ± 0.061
1.968ArgArg: 1.968 ± 0.058
2.152ArgSer: 2.152 ± 0.056
2.148ArgThr: 2.148 ± 0.06
2.693ArgVal: 2.693 ± 0.069
0.388ArgTrp: 0.388 ± 0.023
1.7ArgTyr: 1.7 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
3.803SerAla: 3.803 ± 0.081
0.363SerCys: 0.363 ± 0.026
3.228SerAsp: 3.228 ± 0.073
3.9SerGlu: 3.9 ± 0.08
3.298SerPhe: 3.298 ± 0.076
4.269SerGly: 4.269 ± 0.082
1.102SerHis: 1.102 ± 0.039
4.734SerIle: 4.734 ± 0.099
3.771SerLys: 3.771 ± 0.08
6.324SerLeu: 6.324 ± 0.11
1.563SerMet: 1.563 ± 0.047
2.511SerAsn: 2.511 ± 0.064
1.807SerPro: 1.807 ± 0.052
2.522SerGln: 2.522 ± 0.063
2.238SerArg: 2.238 ± 0.054
4.016SerSer: 4.016 ± 0.123
3.457SerThr: 3.457 ± 0.098
4.157SerVal: 4.157 ± 0.083
0.608SerTrp: 0.608 ± 0.031
2.477SerTyr: 2.477 ± 0.06
0.0SerXaa: 0.0 ± 0.0
Thr
4.204ThrAla: 4.204 ± 0.097
0.316ThrCys: 0.316 ± 0.023
3.171ThrAsp: 3.171 ± 0.071
3.628ThrGlu: 3.628 ± 0.09
2.878ThrPhe: 2.878 ± 0.063
3.989ThrGly: 3.989 ± 0.077
1.261ThrHis: 1.261 ± 0.047
5.364ThrIle: 5.364 ± 0.088
3.973ThrLys: 3.973 ± 0.08
5.847ThrLeu: 5.847 ± 0.099
1.439ThrMet: 1.439 ± 0.052
2.853ThrAsn: 2.853 ± 0.062
2.32ThrPro: 2.32 ± 0.061
2.168ThrGln: 2.168 ± 0.064
1.907ThrArg: 1.907 ± 0.052
3.67ThrSer: 3.67 ± 0.093
4.042ThrThr: 4.042 ± 0.12
4.5ThrVal: 4.5 ± 0.09
0.461ThrTrp: 0.461 ± 0.027
2.264ThrTyr: 2.264 ± 0.059
0.0ThrXaa: 0.0 ± 0.0
Val
5.927ValAla: 5.927 ± 0.112
0.567ValCys: 0.567 ± 0.032
3.809ValAsp: 3.809 ± 0.077
4.278ValGlu: 4.278 ± 0.072
3.081ValPhe: 3.081 ± 0.07
4.739ValGly: 4.739 ± 0.091
1.258ValHis: 1.258 ± 0.043
5.312ValIle: 5.312 ± 0.104
4.395ValLys: 4.395 ± 0.087
6.901ValLeu: 6.901 ± 0.116
1.793ValMet: 1.793 ± 0.052
2.941ValAsn: 2.941 ± 0.07
2.625ValPro: 2.625 ± 0.068
2.697ValGln: 2.697 ± 0.062
2.582ValArg: 2.582 ± 0.06
4.731ValSer: 4.731 ± 0.09
4.624ValThr: 4.624 ± 0.081
5.35ValVal: 5.35 ± 0.105
0.567ValTrp: 0.567 ± 0.029
2.317ValTyr: 2.317 ± 0.063
0.0ValXaa: 0.0 ± 0.0
Trp
0.483TrpAla: 0.483 ± 0.026
0.064TrpCys: 0.064 ± 0.009
0.435TrpAsp: 0.435 ± 0.026
0.525TrpGlu: 0.525 ± 0.027
0.474TrpPhe: 0.474 ± 0.03
0.534TrpGly: 0.534 ± 0.028
0.243TrpHis: 0.243 ± 0.018
0.652TrpIle: 0.652 ± 0.03
0.515TrpLys: 0.515 ± 0.034
1.216TrpLeu: 1.216 ± 0.047
0.273TrpMet: 0.273 ± 0.021
0.448TrpAsn: 0.448 ± 0.027
0.23TrpPro: 0.23 ± 0.02
0.697TrpGln: 0.697 ± 0.031
0.4TrpArg: 0.4 ± 0.023
0.628TrpSer: 0.628 ± 0.033
0.453TrpThr: 0.453 ± 0.024
0.598TrpVal: 0.598 ± 0.031
0.111TrpTrp: 0.111 ± 0.012
0.347TrpTyr: 0.347 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.466TyrAla: 2.466 ± 0.059
0.261TyrCys: 0.261 ± 0.018
2.078TyrAsp: 2.078 ± 0.057
2.436TyrGlu: 2.436 ± 0.058
1.979TyrPhe: 1.979 ± 0.062
2.406TyrGly: 2.406 ± 0.07
0.834TyrHis: 0.834 ± 0.035
2.126TyrIle: 2.126 ± 0.051
1.786TyrLys: 1.786 ± 0.06
4.01TyrLeu: 4.01 ± 0.078
0.782TyrMet: 0.782 ± 0.034
1.43TyrAsn: 1.43 ± 0.051
1.49TyrPro: 1.49 ± 0.047
2.356TyrGln: 2.356 ± 0.071
1.804TyrArg: 1.804 ± 0.057
2.063TyrSer: 2.063 ± 0.057
1.942TyrThr: 1.942 ± 0.055
2.361TyrVal: 2.361 ± 0.056
0.405TyrTrp: 0.405 ± 0.028
1.586TyrTyr: 1.586 ± 0.051
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2404 proteins (685835 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski