Sporolactobacillus laevolacticus DSM 442

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Sporolactobacillaceae; Sporolactobacillus; Sporolactobacillus laevolacticus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3497 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V6IWU9|V6IWU9_9BACL N-acetyltransferase OS=Sporolactobacillus laevolacticus DSM 442 OX=1395513 GN=P343_10910 PE=4 SV=1
MM1 pKa = 7.29NGKK4 pKa = 8.96EE5 pKa = 3.81MNMTEE10 pKa = 4.73KK11 pKa = 10.21YY12 pKa = 9.46TIVDD16 pKa = 3.49KK17 pKa = 9.91DD18 pKa = 3.47TCIACGACAATAPDD32 pKa = 4.02LFDD35 pKa = 4.84YY36 pKa = 10.93DD37 pKa = 4.93DD38 pKa = 4.48EE39 pKa = 5.7GLAFALLDD47 pKa = 3.8KK48 pKa = 11.24NKK50 pKa = 10.68GNVAVPDD57 pKa = 3.85EE58 pKa = 4.45LLEE61 pKa = 4.58DD62 pKa = 4.28LADD65 pKa = 4.86AYY67 pKa = 10.58DD68 pKa = 4.03GCPTEE73 pKa = 4.86SIRR76 pKa = 11.84ISEE79 pKa = 4.39SPFNGHH85 pKa = 6.73ADD87 pKa = 3.63QPVGMM92 pKa = 5.18

Molecular weight: 9.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V6IVH7|V6IVH7_9BACL PTS mannnose transporter subunit IID OS=Sporolactobacillus laevolacticus DSM 442 OX=1395513 GN=P343_13415 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.45GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 9.03VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3497

0

3497

987640

29

2363

282.4

31.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.512 ± 0.041

0.861 ± 0.015

5.336 ± 0.033

6.207 ± 0.048

4.55 ± 0.034

6.858 ± 0.042

2.288 ± 0.019

7.543 ± 0.041

6.584 ± 0.04

10.004 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.737 ± 0.02

4.296 ± 0.031

3.694 ± 0.026

3.949 ± 0.026

4.494 ± 0.031

6.356 ± 0.033

5.378 ± 0.032

6.844 ± 0.035

1.042 ± 0.018

3.468 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski