Weissella halotolerans DSM 20190

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Weissella; Weissella halotolerans

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1304 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R2FZ71|A0A0R2FZ71_9LACO Nudix hydrolase domain-containing protein OS=Weissella halotolerans DSM 20190 OX=1123500 GN=IV68_GL000291 PE=3 SV=1
MM1 pKa = 7.72NYY3 pKa = 7.62QTFARR8 pKa = 11.84LYY10 pKa = 9.65DD11 pKa = 3.45QLFDD15 pKa = 3.67EE16 pKa = 5.02EE17 pKa = 5.96AYY19 pKa = 10.36EE20 pKa = 4.19DD21 pKa = 3.53WLAYY25 pKa = 8.89AHH27 pKa = 5.95QVIRR31 pKa = 11.84QPEE34 pKa = 4.5GPLLEE39 pKa = 4.42LAGGAGRR46 pKa = 11.84LAIMMKK52 pKa = 8.55QTGFPEE58 pKa = 4.15VMNFDD63 pKa = 4.15LSEE66 pKa = 4.1EE67 pKa = 4.17MLTLAAEE74 pKa = 4.6HH75 pKa = 6.03AQEE78 pKa = 5.41AGVQLPLIQGDD89 pKa = 3.52MRR91 pKa = 11.84EE92 pKa = 4.01WSGLEE97 pKa = 3.83LTFQTITCFADD108 pKa = 3.27SLNYY112 pKa = 10.39LADD115 pKa = 3.93EE116 pKa = 4.19QALLQTFQQVAQHH129 pKa = 6.1LVVGGQFIFDD139 pKa = 4.44VITPKK144 pKa = 9.23QTDD147 pKa = 4.22DD148 pKa = 3.34IYY150 pKa = 10.87PGYY153 pKa = 9.25MYY155 pKa = 10.7NWHH158 pKa = 7.39DD159 pKa = 3.78EE160 pKa = 3.91QTAFMWSSFAVPEE173 pKa = 4.04VAHH176 pKa = 5.94QIEE179 pKa = 4.5HH180 pKa = 6.66EE181 pKa = 4.41LTFFMYY187 pKa = 10.38QEE189 pKa = 4.92EE190 pKa = 4.33IDD192 pKa = 4.87GYY194 pKa = 8.54QQVQEE199 pKa = 3.98FHH201 pKa = 6.32MEE203 pKa = 3.68RR204 pKa = 11.84TYY206 pKa = 11.33PLATYY211 pKa = 9.79QHH213 pKa = 7.54LLTKK217 pKa = 10.6AGFSNIQVSANYY229 pKa = 9.71GKK231 pKa = 10.13QDD233 pKa = 3.95DD234 pKa = 4.29LAGATRR240 pKa = 11.84WFFNVTKK247 pKa = 10.22EE248 pKa = 3.89

Molecular weight: 28.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R2G5Z5|A0A0R2G5Z5_9LACO Purine-cytosine permease related protein OS=Weissella halotolerans DSM 20190 OX=1123500 GN=IV68_GL000395 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.34KK9 pKa = 7.47RR10 pKa = 11.84HH11 pKa = 5.71RR12 pKa = 11.84EE13 pKa = 3.7RR14 pKa = 11.84VHH16 pKa = 6.23GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.01VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1304

0

1304

402550

44

1433

308.7

34.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.696 ± 0.066

0.225 ± 0.012

5.784 ± 0.06

5.246 ± 0.062

3.94 ± 0.048

7.006 ± 0.055

2.198 ± 0.03

6.187 ± 0.053

5.271 ± 0.057

10.502 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.781 ± 0.035

3.834 ± 0.042

3.938 ± 0.036

5.542 ± 0.066

4.487 ± 0.061

5.078 ± 0.05

6.194 ± 0.053

7.547 ± 0.059

1.162 ± 0.032

3.34 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski