Amino acid dipepetide frequency for Staphylococcus phage Henu2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.372AlaAla: 0.372 ± 0.172
0.372AlaCys: 0.372 ± 0.15
2.008AlaAsp: 2.008 ± 0.359
4.538AlaGlu: 4.538 ± 0.568
2.976AlaPhe: 2.976 ± 0.545
3.571AlaGly: 3.571 ± 0.652
0.893AlaHis: 0.893 ± 0.241
4.91AlaIle: 4.91 ± 0.679
5.802AlaLys: 5.802 ± 0.679
4.835AlaLeu: 4.835 ± 0.794
1.488AlaMet: 1.488 ± 0.411
4.463AlaAsn: 4.463 ± 0.526
2.008AlaPro: 2.008 ± 0.35
2.827AlaGln: 2.827 ± 0.493
2.306AlaArg: 2.306 ± 0.378
4.017AlaSer: 4.017 ± 0.737
4.091AlaThr: 4.091 ± 0.585
3.124AlaVal: 3.124 ± 0.678
0.669AlaTrp: 0.669 ± 0.307
2.529AlaTyr: 2.529 ± 0.444
0.0AlaXaa: 0.0 ± 0.0
Cys
0.149CysAla: 0.149 ± 0.096
0.074CysCys: 0.074 ± 0.068
0.223CysAsp: 0.223 ± 0.132
0.149CysGlu: 0.149 ± 0.113
0.149CysPhe: 0.149 ± 0.097
0.298CysGly: 0.298 ± 0.138
0.0CysHis: 0.0 ± 0.0
0.223CysIle: 0.223 ± 0.115
0.446CysLys: 0.446 ± 0.201
0.149CysLeu: 0.149 ± 0.116
0.074CysMet: 0.074 ± 0.08
0.223CysAsn: 0.223 ± 0.133
0.223CysPro: 0.223 ± 0.165
0.223CysGln: 0.223 ± 0.116
0.298CysArg: 0.298 ± 0.15
0.521CysSer: 0.521 ± 0.203
0.149CysThr: 0.149 ± 0.102
0.149CysVal: 0.149 ± 0.123
0.074CysTrp: 0.074 ± 0.069
0.446CysTyr: 0.446 ± 0.165
0.0CysXaa: 0.0 ± 0.0
Asp
4.166AspAla: 4.166 ± 0.579
0.074AspCys: 0.074 ± 0.089
4.091AspAsp: 4.091 ± 0.765
5.282AspGlu: 5.282 ± 0.682
3.273AspPhe: 3.273 ± 0.522
4.538AspGly: 4.538 ± 0.549
0.521AspHis: 0.521 ± 0.168
4.761AspIle: 4.761 ± 0.675
5.728AspLys: 5.728 ± 0.81
5.207AspLeu: 5.207 ± 0.571
1.562AspMet: 1.562 ± 0.279
4.017AspAsn: 4.017 ± 0.623
1.19AspPro: 1.19 ± 0.288
0.893AspGln: 0.893 ± 0.221
1.562AspArg: 1.562 ± 0.337
3.794AspSer: 3.794 ± 0.653
3.273AspThr: 3.273 ± 0.448
4.091AspVal: 4.091 ± 0.645
0.521AspTrp: 0.521 ± 0.214
3.05AspTyr: 3.05 ± 0.441
0.0AspXaa: 0.0 ± 0.0
Glu
4.91GluAla: 4.91 ± 0.615
0.744GluCys: 0.744 ± 0.278
4.24GluAsp: 4.24 ± 0.651
7.067GluGlu: 7.067 ± 1.226
2.976GluPhe: 2.976 ± 0.54
2.604GluGly: 2.604 ± 0.4
1.488GluHis: 1.488 ± 0.373
6.472GluIle: 6.472 ± 0.935
5.207GluLys: 5.207 ± 0.878
7.96GluLeu: 7.96 ± 0.974
1.785GluMet: 1.785 ± 0.427
4.538GluAsn: 4.538 ± 0.639
1.934GluPro: 1.934 ± 0.373
4.612GluGln: 4.612 ± 0.644
3.124GluArg: 3.124 ± 0.603
3.943GluSer: 3.943 ± 0.561
3.199GluThr: 3.199 ± 0.412
5.356GluVal: 5.356 ± 0.471
1.041GluTrp: 1.041 ± 0.287
4.017GluTyr: 4.017 ± 0.65
0.0GluXaa: 0.0 ± 0.0
Phe
2.008PheAla: 2.008 ± 0.384
0.074PheCys: 0.074 ± 0.069
3.496PheAsp: 3.496 ± 0.442
4.091PheGlu: 4.091 ± 0.702
1.488PhePhe: 1.488 ± 0.322
2.529PheGly: 2.529 ± 0.628
0.521PheHis: 0.521 ± 0.189
3.273PheIle: 3.273 ± 0.5
4.984PheLys: 4.984 ± 0.499
2.752PheLeu: 2.752 ± 0.54
0.744PheMet: 0.744 ± 0.21
3.347PheAsn: 3.347 ± 0.5
0.818PhePro: 0.818 ± 0.237
1.265PheGln: 1.265 ± 0.278
1.265PheArg: 1.265 ± 0.288
2.455PheSer: 2.455 ± 0.438
2.306PheThr: 2.306 ± 0.51
2.604PheVal: 2.604 ± 0.487
0.446PheTrp: 0.446 ± 0.185
1.488PheTyr: 1.488 ± 0.325
0.0PheXaa: 0.0 ± 0.0
Gly
4.538GlyAla: 4.538 ± 0.656
0.372GlyCys: 0.372 ± 0.149
3.422GlyAsp: 3.422 ± 0.704
2.678GlyGlu: 2.678 ± 0.496
2.306GlyPhe: 2.306 ± 0.471
3.199GlyGly: 3.199 ± 0.516
1.488GlyHis: 1.488 ± 0.383
4.463GlyIle: 4.463 ± 0.548
4.091GlyLys: 4.091 ± 0.433
4.984GlyLeu: 4.984 ± 0.696
1.19GlyMet: 1.19 ± 0.298
3.199GlyAsn: 3.199 ± 0.526
0.521GlyPro: 0.521 ± 0.181
2.752GlyGln: 2.752 ± 0.358
2.306GlyArg: 2.306 ± 0.403
3.124GlySer: 3.124 ± 0.506
4.017GlyThr: 4.017 ± 0.647
4.166GlyVal: 4.166 ± 0.693
1.339GlyTrp: 1.339 ± 0.423
2.827GlyTyr: 2.827 ± 0.418
0.0GlyXaa: 0.0 ± 0.0
His
1.041HisAla: 1.041 ± 0.219
0.0HisCys: 0.0 ± 0.0
0.669HisAsp: 0.669 ± 0.228
1.265HisGlu: 1.265 ± 0.378
0.893HisPhe: 0.893 ± 0.228
1.413HisGly: 1.413 ± 0.257
0.446HisHis: 0.446 ± 0.188
1.041HisIle: 1.041 ± 0.259
0.967HisLys: 0.967 ± 0.268
1.265HisLeu: 1.265 ± 0.317
0.223HisMet: 0.223 ± 0.113
0.893HisAsn: 0.893 ± 0.284
0.744HisPro: 0.744 ± 0.21
0.818HisGln: 0.818 ± 0.28
0.744HisArg: 0.744 ± 0.241
0.967HisSer: 0.967 ± 0.217
1.711HisThr: 1.711 ± 0.365
0.893HisVal: 0.893 ± 0.266
0.074HisTrp: 0.074 ± 0.066
0.967HisTyr: 0.967 ± 0.402
0.0HisXaa: 0.0 ± 0.0
Ile
4.24IleAla: 4.24 ± 0.595
0.0IleCys: 0.0 ± 0.0
5.951IleAsp: 5.951 ± 0.838
6.621IleGlu: 6.621 ± 0.836
2.455IlePhe: 2.455 ± 0.536
5.058IleGly: 5.058 ± 0.955
1.116IleHis: 1.116 ± 0.252
5.058IleIle: 5.058 ± 0.726
7.96IleLys: 7.96 ± 0.81
4.166IleLeu: 4.166 ± 0.532
2.306IleMet: 2.306 ± 0.33
5.951IleAsn: 5.951 ± 1.156
2.008IlePro: 2.008 ± 0.298
3.496IleGln: 3.496 ± 0.432
3.273IleArg: 3.273 ± 0.563
4.761IleSer: 4.761 ± 0.626
5.282IleThr: 5.282 ± 0.573
3.719IleVal: 3.719 ± 0.478
0.744IleTrp: 0.744 ± 0.293
2.306IleTyr: 2.306 ± 0.499
0.0IleXaa: 0.0 ± 0.0
Lys
4.686LysAla: 4.686 ± 0.562
0.074LysCys: 0.074 ± 0.069
6.546LysAsp: 6.546 ± 0.677
7.662LysGlu: 7.662 ± 0.874
3.571LysPhe: 3.571 ± 0.529
5.058LysGly: 5.058 ± 0.642
1.488LysHis: 1.488 ± 0.291
5.951LysIle: 5.951 ± 0.77
8.852LysLys: 8.852 ± 0.953
6.323LysLeu: 6.323 ± 0.681
2.529LysMet: 2.529 ± 0.415
5.356LysAsn: 5.356 ± 0.635
2.827LysPro: 2.827 ± 0.554
5.133LysGln: 5.133 ± 0.731
5.058LysArg: 5.058 ± 0.664
5.058LysSer: 5.058 ± 0.562
6.546LysThr: 6.546 ± 0.745
4.315LysVal: 4.315 ± 0.566
0.744LysTrp: 0.744 ± 0.264
3.645LysTyr: 3.645 ± 0.583
0.0LysXaa: 0.0 ± 0.0
Leu
3.868LeuAla: 3.868 ± 0.662
0.372LeuCys: 0.372 ± 0.195
5.505LeuAsp: 5.505 ± 0.459
6.918LeuGlu: 6.918 ± 0.74
3.719LeuPhe: 3.719 ± 0.447
3.496LeuGly: 3.496 ± 0.539
1.116LeuHis: 1.116 ± 0.315
5.282LeuIle: 5.282 ± 0.571
7.216LeuLys: 7.216 ± 0.823
4.686LeuLeu: 4.686 ± 0.658
1.86LeuMet: 1.86 ± 0.428
4.984LeuAsn: 4.984 ± 0.576
2.232LeuPro: 2.232 ± 0.37
3.347LeuGln: 3.347 ± 0.425
2.901LeuArg: 2.901 ± 0.539
5.133LeuSer: 5.133 ± 0.661
5.505LeuThr: 5.505 ± 0.712
3.571LeuVal: 3.571 ± 0.483
0.595LeuTrp: 0.595 ± 0.248
3.199LeuTyr: 3.199 ± 0.54
0.0LeuXaa: 0.0 ± 0.0
Met
1.488MetAla: 1.488 ± 0.482
0.074MetCys: 0.074 ± 0.068
1.488MetAsp: 1.488 ± 0.256
1.86MetGlu: 1.86 ± 0.371
0.967MetPhe: 0.967 ± 0.255
1.041MetGly: 1.041 ± 0.287
0.223MetHis: 0.223 ± 0.126
1.488MetIle: 1.488 ± 0.324
1.711MetLys: 1.711 ± 0.338
2.232MetLeu: 2.232 ± 0.379
0.893MetMet: 0.893 ± 0.267
1.19MetAsn: 1.19 ± 0.252
0.818MetPro: 0.818 ± 0.252
1.711MetGln: 1.711 ± 0.475
0.967MetArg: 0.967 ± 0.247
1.488MetSer: 1.488 ± 0.445
2.306MetThr: 2.306 ± 0.372
1.041MetVal: 1.041 ± 0.26
0.446MetTrp: 0.446 ± 0.179
1.116MetTyr: 1.116 ± 0.378
0.0MetXaa: 0.0 ± 0.0
Asn
5.133AsnAla: 5.133 ± 0.675
0.074AsnCys: 0.074 ± 0.079
4.761AsnAsp: 4.761 ± 0.541
5.282AsnGlu: 5.282 ± 0.781
3.05AsnPhe: 3.05 ± 0.524
4.091AsnGly: 4.091 ± 0.636
0.893AsnHis: 0.893 ± 0.245
4.984AsnIle: 4.984 ± 0.645
6.397AsnLys: 6.397 ± 0.8
4.091AsnLeu: 4.091 ± 0.563
1.041AsnMet: 1.041 ± 0.284
4.761AsnAsn: 4.761 ± 0.847
2.827AsnPro: 2.827 ± 0.448
3.422AsnGln: 3.422 ± 0.685
2.232AsnArg: 2.232 ± 0.327
2.827AsnSer: 2.827 ± 0.374
3.868AsnThr: 3.868 ± 0.418
3.347AsnVal: 3.347 ± 0.485
1.041AsnTrp: 1.041 ± 0.207
2.38AsnTyr: 2.38 ± 0.473
0.0AsnXaa: 0.0 ± 0.0
Pro
1.785ProAla: 1.785 ± 0.374
0.074ProCys: 0.074 ± 0.078
1.265ProAsp: 1.265 ± 0.273
1.413ProGlu: 1.413 ± 0.275
1.413ProPhe: 1.413 ± 0.341
1.785ProGly: 1.785 ± 0.406
0.521ProHis: 0.521 ± 0.188
2.306ProIle: 2.306 ± 0.499
2.529ProLys: 2.529 ± 0.482
1.711ProLeu: 1.711 ± 0.314
0.818ProMet: 0.818 ± 0.28
1.637ProAsn: 1.637 ± 0.39
0.521ProPro: 0.521 ± 0.171
1.116ProGln: 1.116 ± 0.267
1.265ProArg: 1.265 ± 0.313
1.637ProSer: 1.637 ± 0.437
1.562ProThr: 1.562 ± 0.302
2.008ProVal: 2.008 ± 0.387
0.298ProTrp: 0.298 ± 0.163
1.041ProTyr: 1.041 ± 0.306
0.0ProXaa: 0.0 ± 0.0
Gln
3.347GlnAla: 3.347 ± 0.476
0.298GlnCys: 0.298 ± 0.152
1.488GlnAsp: 1.488 ± 0.364
2.604GlnGlu: 2.604 ± 0.42
2.008GlnPhe: 2.008 ± 0.365
2.976GlnGly: 2.976 ± 0.444
1.413GlnHis: 1.413 ± 0.377
3.719GlnIle: 3.719 ± 0.356
2.976GlnLys: 2.976 ± 0.554
3.571GlnLeu: 3.571 ± 0.498
1.637GlnMet: 1.637 ± 0.359
3.199GlnAsn: 3.199 ± 0.413
1.562GlnPro: 1.562 ± 0.403
2.232GlnGln: 2.232 ± 0.589
2.232GlnArg: 2.232 ± 0.443
3.199GlnSer: 3.199 ± 0.436
1.86GlnThr: 1.86 ± 0.365
2.901GlnVal: 2.901 ± 0.519
0.223GlnTrp: 0.223 ± 0.141
1.637GlnTyr: 1.637 ± 0.401
0.0GlnXaa: 0.0 ± 0.0
Arg
1.785ArgAla: 1.785 ± 0.366
0.298ArgCys: 0.298 ± 0.132
2.604ArgAsp: 2.604 ± 0.494
3.347ArgGlu: 3.347 ± 0.564
2.008ArgPhe: 2.008 ± 0.414
2.306ArgGly: 2.306 ± 0.451
1.041ArgHis: 1.041 ± 0.302
3.496ArgIle: 3.496 ± 0.579
4.315ArgLys: 4.315 ± 0.676
3.273ArgLeu: 3.273 ± 0.576
0.818ArgMet: 0.818 ± 0.215
3.273ArgAsn: 3.273 ± 0.448
0.446ArgPro: 0.446 ± 0.162
1.637ArgGln: 1.637 ± 0.336
1.562ArgArg: 1.562 ± 0.3
2.529ArgSer: 2.529 ± 0.444
1.934ArgThr: 1.934 ± 0.459
2.157ArgVal: 2.157 ± 0.355
0.595ArgTrp: 0.595 ± 0.186
1.785ArgTyr: 1.785 ± 0.354
0.0ArgXaa: 0.0 ± 0.0
Ser
3.868SerAla: 3.868 ± 0.531
0.372SerCys: 0.372 ± 0.191
3.794SerAsp: 3.794 ± 0.581
4.538SerGlu: 4.538 ± 0.602
2.529SerPhe: 2.529 ± 0.422
3.794SerGly: 3.794 ± 0.515
1.265SerHis: 1.265 ± 0.307
5.058SerIle: 5.058 ± 0.675
6.397SerLys: 6.397 ± 0.828
3.199SerLeu: 3.199 ± 0.461
1.637SerMet: 1.637 ± 0.298
4.315SerAsn: 4.315 ± 0.523
0.893SerPro: 0.893 ± 0.288
2.604SerGln: 2.604 ± 0.444
2.604SerArg: 2.604 ± 0.373
3.571SerSer: 3.571 ± 0.65
3.868SerThr: 3.868 ± 0.395
3.645SerVal: 3.645 ± 0.67
0.669SerTrp: 0.669 ± 0.208
2.232SerTyr: 2.232 ± 0.324
0.0SerXaa: 0.0 ± 0.0
Thr
3.868ThrAla: 3.868 ± 0.583
0.223ThrCys: 0.223 ± 0.116
3.571ThrAsp: 3.571 ± 0.394
3.943ThrGlu: 3.943 ± 0.51
2.529ThrPhe: 2.529 ± 0.494
3.347ThrGly: 3.347 ± 0.585
1.19ThrHis: 1.19 ± 0.253
5.505ThrIle: 5.505 ± 0.745
5.579ThrLys: 5.579 ± 0.72
5.728ThrLeu: 5.728 ± 0.629
1.041ThrMet: 1.041 ± 0.271
4.761ThrAsn: 4.761 ± 0.64
1.785ThrPro: 1.785 ± 0.345
2.678ThrGln: 2.678 ± 0.491
2.678ThrArg: 2.678 ± 0.461
5.133ThrSer: 5.133 ± 0.709
4.091ThrThr: 4.091 ± 0.671
2.901ThrVal: 2.901 ± 0.561
0.967ThrTrp: 0.967 ± 0.304
2.306ThrTyr: 2.306 ± 0.414
0.0ThrXaa: 0.0 ± 0.0
Val
4.24ValAla: 4.24 ± 0.844
0.372ValCys: 0.372 ± 0.162
4.315ValAsp: 4.315 ± 0.764
3.571ValGlu: 3.571 ± 0.559
1.711ValPhe: 1.711 ± 0.373
2.976ValGly: 2.976 ± 0.517
0.521ValHis: 0.521 ± 0.193
4.091ValIle: 4.091 ± 0.491
5.728ValLys: 5.728 ± 0.591
4.463ValLeu: 4.463 ± 0.631
1.711ValMet: 1.711 ± 0.399
3.347ValAsn: 3.347 ± 0.432
2.157ValPro: 2.157 ± 0.47
1.711ValGln: 1.711 ± 0.392
2.306ValArg: 2.306 ± 0.324
3.273ValSer: 3.273 ± 0.546
4.686ValThr: 4.686 ± 0.551
3.199ValVal: 3.199 ± 0.547
0.744ValTrp: 0.744 ± 0.253
2.008ValTyr: 2.008 ± 0.451
0.0ValXaa: 0.0 ± 0.0
Trp
0.521TrpAla: 0.521 ± 0.199
0.074TrpCys: 0.074 ± 0.069
0.298TrpAsp: 0.298 ± 0.169
1.116TrpGlu: 1.116 ± 0.231
0.372TrpPhe: 0.372 ± 0.153
0.818TrpGly: 0.818 ± 0.324
0.223TrpHis: 0.223 ± 0.131
0.818TrpIle: 0.818 ± 0.246
0.967TrpLys: 0.967 ± 0.257
1.339TrpLeu: 1.339 ± 0.341
0.149TrpMet: 0.149 ± 0.103
0.595TrpAsn: 0.595 ± 0.204
0.0TrpPro: 0.0 ± 0.0
0.744TrpGln: 0.744 ± 0.228
0.446TrpArg: 0.446 ± 0.166
0.818TrpSer: 0.818 ± 0.235
0.818TrpThr: 0.818 ± 0.221
1.041TrpVal: 1.041 ± 0.251
0.0TrpTrp: 0.0 ± 0.0
0.595TrpTyr: 0.595 ± 0.223
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.785TyrAla: 1.785 ± 0.394
0.223TyrCys: 0.223 ± 0.126
1.86TyrAsp: 1.86 ± 0.356
3.273TyrGlu: 3.273 ± 0.557
1.562TyrPhe: 1.562 ± 0.35
2.157TyrGly: 2.157 ± 0.581
0.669TyrHis: 0.669 ± 0.221
3.571TyrIle: 3.571 ± 0.601
3.571TyrLys: 3.571 ± 0.515
3.571TyrLeu: 3.571 ± 0.474
0.967TyrMet: 0.967 ± 0.265
2.455TyrAsn: 2.455 ± 0.438
1.19TyrPro: 1.19 ± 0.313
1.86TyrGln: 1.86 ± 0.292
2.083TyrArg: 2.083 ± 0.517
2.604TyrSer: 2.604 ± 0.572
2.752TyrThr: 2.752 ± 0.489
2.901TyrVal: 2.901 ± 0.49
0.521TyrTrp: 0.521 ± 0.204
2.604TyrTyr: 2.604 ± 0.668
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (13444 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski