Lacinutrix sp. (strain 5H-3-7-4)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Lacinutrix; unclassified Lacinutrix

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2958 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6GC29|F6GC29_LACS5 DNA repair protein RadC OS=Lacinutrix sp. (strain 5H-3-7-4) OX=983544 GN=Lacal_0432 PE=3 SV=1
MM1 pKa = 7.53KK2 pKa = 9.96NIKK5 pKa = 10.04YY6 pKa = 10.11IIILLFVAAFNYY18 pKa = 10.37ACEE21 pKa = 4.23NDD23 pKa = 3.66DD24 pKa = 4.17SIEE27 pKa = 4.11RR28 pKa = 11.84RR29 pKa = 11.84GKK31 pKa = 9.47PAITVLNKK39 pKa = 9.56TVTVTEE45 pKa = 4.49GEE47 pKa = 4.29TATFDD52 pKa = 4.57LEE54 pKa = 3.84VDD56 pKa = 3.81YY57 pKa = 11.52AVANPINIRR66 pKa = 11.84IDD68 pKa = 3.48VLDD71 pKa = 4.27DD72 pKa = 3.24QGNPLVVTLPSGAPDD87 pKa = 3.72SGNGLYY93 pKa = 10.76DD94 pKa = 4.09LVTLEE99 pKa = 5.26DD100 pKa = 3.67VFVPYY105 pKa = 9.05NTWFEE110 pKa = 4.14SGYY113 pKa = 9.97FQYY116 pKa = 10.92GYY118 pKa = 9.24TGGTGYY124 pKa = 10.31IANFPAGQTSLQINIEE140 pKa = 4.16TLQDD144 pKa = 4.49FIPNDD149 pKa = 3.81TKK151 pKa = 10.79IVNLKK156 pKa = 8.49FTSTNLLEE164 pKa = 4.07ATIDD168 pKa = 3.61EE169 pKa = 4.42VVSINIEE176 pKa = 4.05NFVSNSLITRR186 pKa = 11.84LDD188 pKa = 2.98WSGDD192 pKa = 3.55YY193 pKa = 10.71LDD195 pKa = 5.67NGINPCDD202 pKa = 3.97GDD204 pKa = 3.8TALDD208 pKa = 4.11LDD210 pKa = 4.83LEE212 pKa = 4.54LYY214 pKa = 10.95YY215 pKa = 11.23NGAFTDD221 pKa = 4.91FSYY224 pKa = 11.06SEE226 pKa = 4.47CPEE229 pKa = 4.03EE230 pKa = 4.72LTILDD235 pKa = 4.0TDD237 pKa = 3.8PDD239 pKa = 3.62GTYY242 pKa = 10.38EE243 pKa = 3.86IDD245 pKa = 3.25ASFWTSNGNTSSTVTNVPTTIIFTKK270 pKa = 10.34PGIFYY275 pKa = 10.65QEE277 pKa = 3.61VDD279 pKa = 3.18ISSVFPLADD288 pKa = 3.84GGLSDD293 pKa = 4.83GNSSAITTFTVTKK306 pKa = 9.83TGTTYY311 pKa = 10.82TVTDD315 pKa = 3.87SNNTQIAQGRR325 pKa = 11.84NSNYY329 pKa = 10.35RR330 pKa = 11.84ITKK333 pKa = 5.51TQKK336 pKa = 10.2KK337 pKa = 7.03QFKK340 pKa = 9.98

Molecular weight: 37.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6GHH9|F6GHH9_LACS5 Gliding motility-associated protein GldE OS=Lacinutrix sp. (strain 5H-3-7-4) OX=983544 GN=Lacal_1224 PE=3 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.32KK6 pKa = 9.59RR7 pKa = 11.84KK8 pKa = 7.05RR9 pKa = 11.84HH10 pKa = 5.11KK11 pKa = 10.59VATHH15 pKa = 5.21KK16 pKa = 10.3RR17 pKa = 11.84KK18 pKa = 9.62KK19 pKa = 9.04RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84ANRR25 pKa = 11.84HH26 pKa = 4.77KK27 pKa = 10.58KK28 pKa = 9.8KK29 pKa = 10.51KK30 pKa = 10.04

Molecular weight: 3.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2958

0

2958

1005557

30

3167

339.9

38.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.414 ± 0.044

0.769 ± 0.021

5.58 ± 0.06

6.461 ± 0.046

5.256 ± 0.042

6.009 ± 0.051

1.636 ± 0.023

8.152 ± 0.042

8.21 ± 0.085

9.101 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.979 ± 0.024

7.065 ± 0.053

3.19 ± 0.032

3.332 ± 0.026

3.044 ± 0.032

6.32 ± 0.038

6.396 ± 0.076

6.118 ± 0.041

0.939 ± 0.017

4.027 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski