Amino acid dipepetide frequency for Pseudomonas phage JG012

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.605AlaAla: 11.605 ± 1.223
1.314AlaCys: 1.314 ± 0.26
5.419AlaAsp: 5.419 ± 0.68
6.076AlaGlu: 6.076 ± 0.854
3.284AlaPhe: 3.284 ± 0.528
7.445AlaGly: 7.445 ± 0.579
1.752AlaHis: 1.752 ± 0.267
4.927AlaIle: 4.927 ± 0.63
5.474AlaLys: 5.474 ± 0.749
9.853AlaLeu: 9.853 ± 0.593
3.065AlaMet: 3.065 ± 0.371
3.996AlaAsn: 3.996 ± 0.378
5.255AlaPro: 5.255 ± 1.24
5.967AlaGln: 5.967 ± 0.848
6.624AlaArg: 6.624 ± 0.702
6.569AlaSer: 6.569 ± 0.639
6.076AlaThr: 6.076 ± 0.616
7.171AlaVal: 7.171 ± 0.654
1.478AlaTrp: 1.478 ± 0.245
3.065AlaTyr: 3.065 ± 0.495
0.0AlaXaa: 0.0 ± 0.0
Cys
0.657CysAla: 0.657 ± 0.186
0.383CysCys: 0.383 ± 0.16
1.095CysAsp: 1.095 ± 0.266
0.766CysGlu: 0.766 ± 0.208
0.547CysPhe: 0.547 ± 0.161
1.15CysGly: 1.15 ± 0.301
0.383CysHis: 0.383 ± 0.148
0.493CysIle: 0.493 ± 0.197
0.712CysLys: 0.712 ± 0.234
0.931CysLeu: 0.931 ± 0.215
0.438CysMet: 0.438 ± 0.143
0.657CysAsn: 0.657 ± 0.197
0.657CysPro: 0.657 ± 0.181
0.328CysGln: 0.328 ± 0.113
1.095CysArg: 1.095 ± 0.28
0.383CysSer: 0.383 ± 0.163
0.493CysThr: 0.493 ± 0.174
0.657CysVal: 0.657 ± 0.186
0.219CysTrp: 0.219 ± 0.108
0.547CysTyr: 0.547 ± 0.179
0.0CysXaa: 0.0 ± 0.0
Asp
6.076AspAla: 6.076 ± 0.507
0.219AspCys: 0.219 ± 0.125
2.847AspAsp: 2.847 ± 0.359
3.777AspGlu: 3.777 ± 0.408
2.025AspPhe: 2.025 ± 0.344
5.365AspGly: 5.365 ± 0.48
1.314AspHis: 1.314 ± 0.274
3.284AspIle: 3.284 ± 0.452
2.901AspLys: 2.901 ± 0.428
5.365AspLeu: 5.365 ± 0.565
1.752AspMet: 1.752 ± 0.224
2.299AspAsn: 2.299 ± 0.323
2.847AspPro: 2.847 ± 0.365
2.08AspGln: 2.08 ± 0.419
2.901AspArg: 2.901 ± 0.382
3.449AspSer: 3.449 ± 0.426
2.573AspThr: 2.573 ± 0.449
3.339AspVal: 3.339 ± 0.4
0.985AspTrp: 0.985 ± 0.243
2.025AspTyr: 2.025 ± 0.304
0.0AspXaa: 0.0 ± 0.0
Glu
7.007GluAla: 7.007 ± 0.895
0.876GluCys: 0.876 ± 0.21
3.12GluAsp: 3.12 ± 0.399
4.325GluGlu: 4.325 ± 0.515
1.916GluPhe: 1.916 ± 0.304
4.325GluGly: 4.325 ± 0.603
1.15GluHis: 1.15 ± 0.246
3.284GluIle: 3.284 ± 0.387
3.449GluLys: 3.449 ± 0.53
6.24GluLeu: 6.24 ± 0.636
2.19GluMet: 2.19 ± 0.341
2.08GluAsn: 2.08 ± 0.376
1.478GluPro: 1.478 ± 0.348
3.503GluGln: 3.503 ± 0.558
3.175GluArg: 3.175 ± 0.493
2.737GluSer: 2.737 ± 0.361
2.682GluThr: 2.682 ± 0.357
4.379GluVal: 4.379 ± 0.465
1.259GluTrp: 1.259 ± 0.279
1.752GluTyr: 1.752 ± 0.269
0.0GluXaa: 0.0 ± 0.0
Phe
3.777PheAla: 3.777 ± 0.535
0.274PheCys: 0.274 ± 0.135
2.682PheAsp: 2.682 ± 0.275
2.08PheGlu: 2.08 ± 0.319
1.423PhePhe: 1.423 ± 0.283
2.463PheGly: 2.463 ± 0.367
0.876PheHis: 0.876 ± 0.182
1.533PheIle: 1.533 ± 0.281
1.642PheLys: 1.642 ± 0.345
2.573PheLeu: 2.573 ± 0.356
1.04PheMet: 1.04 ± 0.297
2.135PheAsn: 2.135 ± 0.259
1.697PhePro: 1.697 ± 0.29
1.204PheGln: 1.204 ± 0.205
2.299PheArg: 2.299 ± 0.329
1.806PheSer: 1.806 ± 0.371
1.806PheThr: 1.806 ± 0.407
2.792PheVal: 2.792 ± 0.341
0.602PheTrp: 0.602 ± 0.185
0.766PheTyr: 0.766 ± 0.213
0.0PheXaa: 0.0 ± 0.0
Gly
7.664GlyAla: 7.664 ± 0.723
0.712GlyCys: 0.712 ± 0.235
3.503GlyAsp: 3.503 ± 0.374
4.106GlyGlu: 4.106 ± 0.593
3.996GlyPhe: 3.996 ± 0.518
5.967GlyGly: 5.967 ± 0.689
1.752GlyHis: 1.752 ± 0.35
3.941GlyIle: 3.941 ± 0.511
4.817GlyLys: 4.817 ± 0.486
5.693GlyLeu: 5.693 ± 0.553
2.244GlyMet: 2.244 ± 0.412
2.025GlyAsn: 2.025 ± 0.262
2.19GlyPro: 2.19 ± 0.334
3.558GlyGln: 3.558 ± 0.491
3.777GlyArg: 3.777 ± 0.496
5.748GlySer: 5.748 ± 0.649
4.927GlyThr: 4.927 ± 0.457
6.405GlyVal: 6.405 ± 0.558
1.15GlyTrp: 1.15 ± 0.311
2.299GlyTyr: 2.299 ± 0.41
0.0GlyXaa: 0.0 ± 0.0
His
1.806HisAla: 1.806 ± 0.315
0.383HisCys: 0.383 ± 0.171
0.985HisAsp: 0.985 ± 0.221
1.15HisGlu: 1.15 ± 0.27
0.712HisPhe: 0.712 ± 0.177
1.04HisGly: 1.04 ± 0.243
0.493HisHis: 0.493 ± 0.196
1.478HisIle: 1.478 ± 0.237
1.095HisLys: 1.095 ± 0.28
1.314HisLeu: 1.314 ± 0.274
0.602HisMet: 0.602 ± 0.194
0.493HisAsn: 0.493 ± 0.176
0.931HisPro: 0.931 ± 0.224
0.274HisGln: 0.274 ± 0.127
1.095HisArg: 1.095 ± 0.264
1.314HisSer: 1.314 ± 0.227
0.985HisThr: 0.985 ± 0.261
1.478HisVal: 1.478 ± 0.39
0.712HisTrp: 0.712 ± 0.243
0.821HisTyr: 0.821 ± 0.218
0.0HisXaa: 0.0 ± 0.0
Ile
5.912IleAla: 5.912 ± 0.584
0.876IleCys: 0.876 ± 0.211
3.941IleAsp: 3.941 ± 0.471
3.23IleGlu: 3.23 ± 0.436
1.259IlePhe: 1.259 ± 0.306
3.777IleGly: 3.777 ± 0.496
1.095IleHis: 1.095 ± 0.235
1.971IleIle: 1.971 ± 0.294
2.19IleLys: 2.19 ± 0.306
3.394IleLeu: 3.394 ± 0.462
1.259IleMet: 1.259 ± 0.229
2.19IleAsn: 2.19 ± 0.295
1.971IlePro: 1.971 ± 0.255
1.971IleGln: 1.971 ± 0.392
3.503IleArg: 3.503 ± 0.411
2.299IleSer: 2.299 ± 0.387
3.449IleThr: 3.449 ± 0.403
3.996IleVal: 3.996 ± 0.484
0.547IleTrp: 0.547 ± 0.199
1.423IleTyr: 1.423 ± 0.288
0.0IleXaa: 0.0 ± 0.0
Lys
5.857LysAla: 5.857 ± 0.915
0.547LysCys: 0.547 ± 0.179
2.354LysAsp: 2.354 ± 0.336
3.941LysGlu: 3.941 ± 0.565
1.971LysPhe: 1.971 ± 0.346
3.503LysGly: 3.503 ± 0.393
0.876LysHis: 0.876 ± 0.219
2.792LysIle: 2.792 ± 0.348
2.628LysLys: 2.628 ± 0.469
4.598LysLeu: 4.598 ± 0.458
1.314LysMet: 1.314 ± 0.275
2.025LysAsn: 2.025 ± 0.316
2.463LysPro: 2.463 ± 0.401
1.642LysGln: 1.642 ± 0.232
3.065LysArg: 3.065 ± 0.432
2.025LysSer: 2.025 ± 0.346
2.847LysThr: 2.847 ± 0.46
3.832LysVal: 3.832 ± 0.369
0.712LysTrp: 0.712 ± 0.202
1.423LysTyr: 1.423 ± 0.257
0.0LysXaa: 0.0 ± 0.0
Leu
7.718LeuAla: 7.718 ± 0.583
1.259LeuCys: 1.259 ± 0.286
6.295LeuAsp: 6.295 ± 0.65
5.638LeuGlu: 5.638 ± 0.548
2.901LeuPhe: 2.901 ± 0.407
5.912LeuGly: 5.912 ± 0.707
2.244LeuHis: 2.244 ± 0.504
3.722LeuIle: 3.722 ± 0.494
4.817LeuLys: 4.817 ± 0.475
6.295LeuLeu: 6.295 ± 0.726
1.752LeuMet: 1.752 ± 0.341
3.12LeuAsn: 3.12 ± 0.323
3.996LeuPro: 3.996 ± 0.436
3.777LeuGln: 3.777 ± 0.496
5.31LeuArg: 5.31 ± 0.469
4.708LeuSer: 4.708 ± 0.452
4.215LeuThr: 4.215 ± 0.412
4.872LeuVal: 4.872 ± 0.539
1.423LeuTrp: 1.423 ± 0.263
1.971LeuTyr: 1.971 ± 0.319
0.0LeuXaa: 0.0 ± 0.0
Met
2.628MetAla: 2.628 ± 0.348
0.274MetCys: 0.274 ± 0.116
1.369MetAsp: 1.369 ± 0.226
1.806MetGlu: 1.806 ± 0.293
0.547MetPhe: 0.547 ± 0.166
2.025MetGly: 2.025 ± 0.4
0.328MetHis: 0.328 ± 0.147
1.259MetIle: 1.259 ± 0.286
1.587MetLys: 1.587 ± 0.29
2.682MetLeu: 2.682 ± 0.463
0.493MetMet: 0.493 ± 0.13
1.423MetAsn: 1.423 ± 0.246
1.806MetPro: 1.806 ± 0.312
1.314MetGln: 1.314 ± 0.3
2.08MetArg: 2.08 ± 0.308
1.478MetSer: 1.478 ± 0.255
2.135MetThr: 2.135 ± 0.328
1.204MetVal: 1.204 ± 0.257
0.493MetTrp: 0.493 ± 0.173
0.602MetTyr: 0.602 ± 0.155
0.0MetXaa: 0.0 ± 0.0
Asn
3.941AsnAla: 3.941 ± 0.489
0.602AsnCys: 0.602 ± 0.192
2.244AsnAsp: 2.244 ± 0.316
2.518AsnGlu: 2.518 ± 0.357
0.985AsnPhe: 0.985 ± 0.218
3.613AsnGly: 3.613 ± 0.426
0.821AsnHis: 0.821 ± 0.191
1.587AsnIle: 1.587 ± 0.258
1.642AsnLys: 1.642 ± 0.246
3.339AsnLeu: 3.339 ± 0.449
0.985AsnMet: 0.985 ± 0.23
2.135AsnAsn: 2.135 ± 0.409
2.409AsnPro: 2.409 ± 0.317
1.369AsnGln: 1.369 ± 0.274
2.628AsnArg: 2.628 ± 0.37
2.025AsnSer: 2.025 ± 0.303
2.573AsnThr: 2.573 ± 0.514
2.08AsnVal: 2.08 ± 0.327
0.931AsnTrp: 0.931 ± 0.192
1.04AsnTyr: 1.04 ± 0.219
0.0AsnXaa: 0.0 ± 0.0
Pro
5.091ProAla: 5.091 ± 0.895
0.602ProCys: 0.602 ± 0.173
3.339ProAsp: 3.339 ± 0.334
2.518ProGlu: 2.518 ± 0.354
1.916ProPhe: 1.916 ± 0.407
2.409ProGly: 2.409 ± 0.328
0.712ProHis: 0.712 ± 0.212
2.628ProIle: 2.628 ± 0.352
1.642ProLys: 1.642 ± 0.288
3.12ProLeu: 3.12 ± 0.355
1.533ProMet: 1.533 ± 0.262
1.971ProAsn: 1.971 ± 0.313
1.916ProPro: 1.916 ± 0.362
2.08ProGln: 2.08 ± 0.741
2.19ProArg: 2.19 ± 0.326
2.901ProSer: 2.901 ± 0.392
3.011ProThr: 3.011 ± 0.406
3.12ProVal: 3.12 ± 0.512
1.204ProTrp: 1.204 ± 0.329
1.478ProTyr: 1.478 ± 0.301
0.0ProXaa: 0.0 ± 0.0
Gln
6.788GlnAla: 6.788 ± 0.9
0.821GlnCys: 0.821 ± 0.232
2.08GlnAsp: 2.08 ± 0.246
2.737GlnGlu: 2.737 ± 0.345
1.642GlnPhe: 1.642 ± 0.346
3.23GlnGly: 3.23 ± 0.654
0.547GlnHis: 0.547 ± 0.166
2.135GlnIle: 2.135 ± 0.383
1.259GlnLys: 1.259 ± 0.251
3.339GlnLeu: 3.339 ± 0.456
1.533GlnMet: 1.533 ± 0.296
2.19GlnAsn: 2.19 ± 0.362
2.244GlnPro: 2.244 ± 0.519
3.503GlnGln: 3.503 ± 0.973
3.175GlnArg: 3.175 ± 0.386
2.463GlnSer: 2.463 ± 0.396
2.628GlnThr: 2.628 ± 0.457
2.354GlnVal: 2.354 ± 0.366
0.931GlnTrp: 0.931 ± 0.197
0.985GlnTyr: 0.985 ± 0.242
0.0GlnXaa: 0.0 ± 0.0
Arg
6.788ArgAla: 6.788 ± 0.655
0.493ArgCys: 0.493 ± 0.19
3.777ArgAsp: 3.777 ± 0.419
3.613ArgGlu: 3.613 ± 0.494
1.806ArgPhe: 1.806 ± 0.355
3.558ArgGly: 3.558 ± 0.504
0.985ArgHis: 0.985 ± 0.255
3.449ArgIle: 3.449 ± 0.359
2.847ArgLys: 2.847 ± 0.347
5.584ArgLeu: 5.584 ± 0.55
1.587ArgMet: 1.587 ± 0.301
1.971ArgAsn: 1.971 ± 0.308
2.518ArgPro: 2.518 ± 0.362
3.23ArgGln: 3.23 ± 0.395
3.668ArgArg: 3.668 ± 0.561
3.668ArgSer: 3.668 ± 0.41
3.941ArgThr: 3.941 ± 0.429
3.339ArgVal: 3.339 ± 0.386
1.259ArgTrp: 1.259 ± 0.274
1.369ArgTyr: 1.369 ± 0.364
0.0ArgXaa: 0.0 ± 0.0
Ser
5.693SerAla: 5.693 ± 0.467
0.712SerCys: 0.712 ± 0.165
2.847SerAsp: 2.847 ± 0.436
2.792SerGlu: 2.792 ± 0.315
2.19SerPhe: 2.19 ± 0.426
6.733SerGly: 6.733 ± 0.573
0.657SerHis: 0.657 ± 0.186
2.901SerIle: 2.901 ± 0.354
2.792SerLys: 2.792 ± 0.369
3.23SerLeu: 3.23 ± 0.437
1.423SerMet: 1.423 ± 0.337
2.025SerAsn: 2.025 ± 0.305
2.244SerPro: 2.244 ± 0.394
2.463SerGln: 2.463 ± 0.424
3.12SerArg: 3.12 ± 0.412
3.175SerSer: 3.175 ± 0.492
3.339SerThr: 3.339 ± 0.453
4.106SerVal: 4.106 ± 0.476
0.931SerTrp: 0.931 ± 0.19
2.463SerTyr: 2.463 ± 0.362
0.0SerXaa: 0.0 ± 0.0
Thr
6.624ThrAla: 6.624 ± 0.704
0.766ThrCys: 0.766 ± 0.177
3.613ThrAsp: 3.613 ± 0.476
2.956ThrGlu: 2.956 ± 0.405
1.971ThrPhe: 1.971 ± 0.283
5.857ThrGly: 5.857 ± 0.691
1.095ThrHis: 1.095 ± 0.312
2.901ThrIle: 2.901 ± 0.472
2.244ThrLys: 2.244 ± 0.363
4.817ThrLeu: 4.817 ± 0.606
1.533ThrMet: 1.533 ± 0.29
2.628ThrAsn: 2.628 ± 0.436
3.23ThrPro: 3.23 ± 0.443
2.025ThrGln: 2.025 ± 0.426
2.518ThrArg: 2.518 ± 0.311
2.901ThrSer: 2.901 ± 0.419
3.996ThrThr: 3.996 ± 0.529
4.708ThrVal: 4.708 ± 0.537
0.766ThrTrp: 0.766 ± 0.202
1.752ThrTyr: 1.752 ± 0.405
0.0ThrXaa: 0.0 ± 0.0
Val
6.131ValAla: 6.131 ± 0.603
0.712ValCys: 0.712 ± 0.212
3.449ValAsp: 3.449 ± 0.44
4.817ValGlu: 4.817 ± 0.509
2.737ValPhe: 2.737 ± 0.426
5.255ValGly: 5.255 ± 0.593
1.204ValHis: 1.204 ± 0.285
3.284ValIle: 3.284 ± 0.44
4.379ValLys: 4.379 ± 0.488
4.872ValLeu: 4.872 ± 0.505
1.697ValMet: 1.697 ± 0.286
2.19ValAsn: 2.19 ± 0.313
3.394ValPro: 3.394 ± 0.386
3.832ValGln: 3.832 ± 0.489
3.941ValArg: 3.941 ± 0.389
3.23ValSer: 3.23 ± 0.436
4.817ValThr: 4.817 ± 0.637
5.255ValVal: 5.255 ± 0.683
1.369ValTrp: 1.369 ± 0.264
2.025ValTyr: 2.025 ± 0.27
0.0ValXaa: 0.0 ± 0.0
Trp
2.025TrpAla: 2.025 ± 0.282
0.547TrpCys: 0.547 ± 0.185
1.204TrpAsp: 1.204 ± 0.265
0.766TrpGlu: 0.766 ± 0.222
0.493TrpPhe: 0.493 ± 0.186
0.602TrpGly: 0.602 ± 0.17
0.328TrpHis: 0.328 ± 0.127
0.876TrpIle: 0.876 ± 0.198
0.876TrpLys: 0.876 ± 0.253
1.916TrpLeu: 1.916 ± 0.339
0.328TrpMet: 0.328 ± 0.129
0.876TrpAsn: 0.876 ± 0.189
0.712TrpPro: 0.712 ± 0.236
0.766TrpGln: 0.766 ± 0.243
1.15TrpArg: 1.15 ± 0.249
0.821TrpSer: 0.821 ± 0.22
0.985TrpThr: 0.985 ± 0.246
1.314TrpVal: 1.314 ± 0.25
0.328TrpTrp: 0.328 ± 0.131
0.876TrpTyr: 0.876 ± 0.235
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.682TyrAla: 2.682 ± 0.348
0.219TyrCys: 0.219 ± 0.102
1.259TyrAsp: 1.259 ± 0.271
1.314TyrGlu: 1.314 ± 0.264
1.15TyrPhe: 1.15 ± 0.223
2.244TyrGly: 2.244 ± 0.409
0.547TyrHis: 0.547 ± 0.169
1.806TyrIle: 1.806 ± 0.304
1.423TyrLys: 1.423 ± 0.277
2.792TyrLeu: 2.792 ± 0.343
0.712TyrMet: 0.712 ± 0.18
1.04TyrAsn: 1.04 ± 0.199
1.423TyrPro: 1.423 ± 0.22
1.697TyrGln: 1.697 ± 0.305
2.135TyrArg: 2.135 ± 0.318
2.135TyrSer: 2.135 ± 0.356
1.423TyrThr: 1.423 ± 0.247
2.19TyrVal: 2.19 ± 0.438
0.493TyrTrp: 0.493 ± 0.242
0.712TyrTyr: 0.712 ± 0.199
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (18269 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski