Streptococcus satellite phage Javan323

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZLN2|A0A4D5ZLN2_9VIRU DNA primase OS=Streptococcus satellite phage Javan323 OX=2558636 GN=JavanS323_0016 PE=4 SV=1
MM1 pKa = 7.33EE2 pKa = 5.69LKK4 pKa = 10.37EE5 pKa = 4.2FNNKK9 pKa = 7.35VVRR12 pKa = 11.84ITDD15 pKa = 2.79IDD17 pKa = 3.83GQVFKK22 pKa = 11.11GVALYY27 pKa = 10.32EE28 pKa = 4.64DD29 pKa = 4.46KK30 pKa = 10.92DD31 pKa = 3.85VHH33 pKa = 8.59DD34 pKa = 5.08EE35 pKa = 4.09EE36 pKa = 6.72LDD38 pKa = 3.79GLSVNSGTRR47 pKa = 11.84WIKK50 pKa = 10.69LFEE53 pKa = 4.14NEE55 pKa = 4.03IKK57 pKa = 10.5EE58 pKa = 4.37IEE60 pKa = 4.11ITDD63 pKa = 4.69KK64 pKa = 10.95INQYY68 pKa = 10.92LII70 pKa = 3.87

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZKM6|A0A4D5ZKM6_9VIRU Cys_rich_CPCC domain-containing protein OS=Streptococcus satellite phage Javan323 OX=2558636 GN=JavanS323_0005 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 9.94IAEE5 pKa = 4.22IKK7 pKa = 9.09KK8 pKa = 9.16QNGSTVYY15 pKa = 10.16RR16 pKa = 11.84SKK18 pKa = 11.31VYY20 pKa = 10.86LGVDD24 pKa = 2.84KK25 pKa = 10.65LTGKK29 pKa = 10.04KK30 pKa = 9.55IKK32 pKa = 10.74ANLTARR38 pKa = 11.84TKK40 pKa = 10.67TEE42 pKa = 4.16LKK44 pKa = 10.56KK45 pKa = 10.53KK46 pKa = 10.04AQLAKK51 pKa = 10.9AEE53 pKa = 4.11FQNNGSTKK61 pKa = 10.43KK62 pKa = 9.07EE63 pKa = 4.3TIPLTSYY70 pKa = 11.42EE71 pKa = 4.2EE72 pKa = 4.43VAQLWWEE79 pKa = 4.57SYY81 pKa = 7.5QHH83 pKa = 5.01TVKK86 pKa = 10.72PNTQDD91 pKa = 3.01SVKK94 pKa = 10.42RR95 pKa = 11.84LLANHH100 pKa = 5.62VMPLFGSYY108 pKa = 10.48RR109 pKa = 11.84LDD111 pKa = 3.41NLTTPTIQRR120 pKa = 11.84IVNQLALRR128 pKa = 11.84ANKK131 pKa = 10.05RR132 pKa = 11.84EE133 pKa = 3.95EE134 pKa = 4.26GAFLHH139 pKa = 6.3YY140 pKa = 10.87DD141 pKa = 4.12KK142 pKa = 11.05IHH144 pKa = 6.59ALNKK148 pKa = 10.22RR149 pKa = 11.84ILQYY153 pKa = 11.19AVTMQIIDD161 pKa = 3.61INPARR166 pKa = 11.84EE167 pKa = 3.9VILPRR172 pKa = 11.84KK173 pKa = 9.13IKK175 pKa = 10.15QGRR178 pKa = 11.84NKK180 pKa = 9.76VKK182 pKa = 10.45HH183 pKa = 5.65FNNLEE188 pKa = 3.99LKK190 pKa = 10.51QFLSYY195 pKa = 11.05LEE197 pKa = 4.12EE198 pKa = 4.98ANLAIYY204 pKa = 10.3RR205 pKa = 11.84NIYY208 pKa = 10.05EE209 pKa = 3.68ITLYY213 pKa = 10.92KK214 pKa = 10.38FLLATGCRR222 pKa = 11.84INEE225 pKa = 3.92ALALHH230 pKa = 6.77WSDD233 pKa = 4.46IDD235 pKa = 3.63LHH237 pKa = 6.01NATVNITKK245 pKa = 9.15TLNHH249 pKa = 6.65LGEE252 pKa = 4.45INSPKK257 pKa = 10.27SEE259 pKa = 3.73ASYY262 pKa = 8.95RR263 pKa = 11.84TIDD266 pKa = 4.0IDD268 pKa = 3.64PQTIEE273 pKa = 3.99VLKK276 pKa = 10.61VYY278 pKa = 9.35QKK280 pKa = 10.51RR281 pKa = 11.84QRR283 pKa = 11.84QEE285 pKa = 2.93AWKK288 pKa = 10.19LGRR291 pKa = 11.84TEE293 pKa = 4.07TVVFSDD299 pKa = 6.26FIHH302 pKa = 7.25KK303 pKa = 10.29YY304 pKa = 9.81PNNKK308 pKa = 8.11TLATRR313 pKa = 11.84LNTRR317 pKa = 11.84FKK319 pKa = 10.85HH320 pKa = 6.0AGVPNIGFHH329 pKa = 6.22GFRR332 pKa = 11.84HH333 pKa = 4.87THH335 pKa = 6.58ASLLLNSGIPYY346 pKa = 10.0KK347 pKa = 10.34EE348 pKa = 3.8LQHH351 pKa = 6.73RR352 pKa = 11.84LGHH355 pKa = 5.56STLAMTMDD363 pKa = 4.54TYY365 pKa = 11.85SHH367 pKa = 7.08LSKK370 pKa = 10.76EE371 pKa = 4.21KK372 pKa = 10.8AKK374 pKa = 9.65TAVSFYY380 pKa = 9.94EE381 pKa = 4.13KK382 pKa = 10.49AVSSLL387 pKa = 3.39

Molecular weight: 44.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18

0

18

3556

49

455

197.6

22.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.696 ± 0.46

1.012 ± 0.231

5.287 ± 0.467

8.24 ± 0.568

3.6 ± 0.46

5.399 ± 0.417

1.912 ± 0.3

8.746 ± 0.738

9.055 ± 0.704

8.774 ± 0.462

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.603 ± 0.197

5.512 ± 0.538

3.065 ± 0.357

3.6 ± 0.291

3.881 ± 0.551

8.015 ± 0.884

6.58 ± 0.656

5.849 ± 0.428

0.984 ± 0.179

4.19 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski