Mycobacterium phage Myxus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140IEW2|A0A140IEW2_9CAUD Uncharacterized protein OS=Mycobacterium phage Myxus OX=1805458 GN=PBI_MYXUS_91 PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 5.18PQGQIYY8 pKa = 8.83VQDD11 pKa = 3.71MDD13 pKa = 3.98DD14 pKa = 3.32VDD16 pKa = 5.48RR17 pKa = 11.84IGAAPKK23 pKa = 10.09KK24 pKa = 10.3GRR26 pKa = 11.84EE27 pKa = 4.1PGDD30 pKa = 3.14NSYY33 pKa = 11.25NVSWACAALSAYY45 pKa = 10.22AKK47 pKa = 10.64VIGGHH52 pKa = 5.98DD53 pKa = 3.8EE54 pKa = 4.21GVEE57 pKa = 4.05TAISDD62 pKa = 3.95LLGDD66 pKa = 4.64LMHH69 pKa = 7.1LADD72 pKa = 3.97AAGVDD77 pKa = 4.38FEE79 pKa = 5.07EE80 pKa = 4.65MVNKK84 pKa = 10.62AEE86 pKa = 3.97YY87 pKa = 10.59NYY89 pKa = 10.63GFEE92 pKa = 5.12LNGEE96 pKa = 4.22

Molecular weight: 10.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A127KP50|A0A127KP50_9CAUD Uncharacterized protein OS=Mycobacterium phage Myxus OX=1805458 GN=PBI_MYXUS_20 PE=4 SV=1
MM1 pKa = 7.35SWADD5 pKa = 2.94SDD7 pKa = 4.61RR8 pKa = 11.84RR9 pKa = 11.84QNLPADD15 pKa = 3.51WEE17 pKa = 4.64LIRR20 pKa = 11.84LEE22 pKa = 4.28VLHH25 pKa = 6.77DD26 pKa = 4.69ANWLCQIGMAGCTRR40 pKa = 11.84MATDD44 pKa = 2.88VDD46 pKa = 4.33HH47 pKa = 7.44IKK49 pKa = 10.61RR50 pKa = 11.84GNDD53 pKa = 2.81HH54 pKa = 6.12SRR56 pKa = 11.84RR57 pKa = 11.84NLRR60 pKa = 11.84AACGWCHH67 pKa = 6.24DD68 pKa = 4.39RR69 pKa = 11.84KK70 pKa = 10.78SSAEE74 pKa = 3.67GVARR78 pKa = 11.84RR79 pKa = 11.84RR80 pKa = 11.84EE81 pKa = 3.88LKK83 pKa = 10.28ARR85 pKa = 11.84RR86 pKa = 11.84KK87 pKa = 9.71RR88 pKa = 11.84PPEE91 pKa = 3.6RR92 pKa = 11.84HH93 pKa = 5.7PGRR96 pKa = 11.84RR97 pKa = 3.38

Molecular weight: 11.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

99

0

99

16715

27

969

168.8

18.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.063 ± 0.335

0.867 ± 0.109

6.288 ± 0.209

6.539 ± 0.286

3.231 ± 0.18

8.872 ± 0.508

1.855 ± 0.148

5.019 ± 0.199

4.702 ± 0.223

7.951 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.555 ± 0.144

3.267 ± 0.194

5.522 ± 0.313

3.721 ± 0.198

6.198 ± 0.34

5.534 ± 0.212

6.025 ± 0.227

7.03 ± 0.213

1.891 ± 0.124

2.872 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski