Pseudomonas sp. HAR-UPW-AIA-41

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3419 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A2IR97|A0A2A2IR97_9PSED Ribosomal RNA large subunit methyltransferase G OS=Pseudomonas sp. HAR-UPW-AIA-41 OX=1985301 GN=rlmG PE=3 SV=1
GG1 pKa = 6.48QSAVLTIPYY10 pKa = 8.65TVTDD14 pKa = 3.92DD15 pKa = 3.31QGAISTANLVITVTGTNDD33 pKa = 3.05LSVISSASVDD43 pKa = 3.9VIEE46 pKa = 5.79SNASLNTGGTLSISDD61 pKa = 3.7IDD63 pKa = 4.11GPAPSFNPATNASGSGGYY81 pKa = 10.78GLFNLGSNGVWSYY94 pKa = 10.75STTGPLNQLVAGQSYY109 pKa = 9.9TDD111 pKa = 3.68TLTVTAADD119 pKa = 4.13GSTGTVTVTILGTNDD134 pKa = 3.03APVLGGVRR142 pKa = 11.84TSFTYY147 pKa = 10.37SEE149 pKa = 4.39SAASVITRR157 pKa = 11.84TLLDD161 pKa = 3.44SSVTLTDD168 pKa = 3.22IDD170 pKa = 3.92SSNFDD175 pKa = 3.41GGSVIVSISNGVATQDD191 pKa = 3.29QLTVQDD197 pKa = 3.76QGTGSGQISVVGSDD211 pKa = 2.91VRR213 pKa = 11.84YY214 pKa = 9.97NFGSGAVSIGTISGGTGGTPLTISLNSNATPVAVDD249 pKa = 3.61ALVQRR254 pKa = 11.84IAYY257 pKa = 8.21GNSSQLPTTTPRR269 pKa = 11.84TISITVNDD277 pKa = 4.12GDD279 pKa = 4.18GNANGGSSSVTQNITMNVTSVNDD302 pKa = 3.46APTFSNLGGARR313 pKa = 11.84AFTEE317 pKa = 4.17GGNAVVLDD325 pKa = 4.22NNASLGDD332 pKa = 3.65RR333 pKa = 11.84EE334 pKa = 4.34LAVLGDD340 pKa = 3.83FGGASLTLSRR350 pKa = 11.84RR351 pKa = 11.84GGTNSDD357 pKa = 3.28DD358 pKa = 4.09DD359 pKa = 4.22FRR361 pKa = 11.84GTGNLSLSAGEE372 pKa = 4.07VRR374 pKa = 11.84LSGVLVGSYY383 pKa = 10.52DD384 pKa = 3.8QAALTNGTLQITFNSGTSNAQANSVLRR411 pKa = 11.84QIAYY415 pKa = 10.74SNGSDD420 pKa = 3.6APPASVVIDD429 pKa = 3.5YY430 pKa = 11.05ALNDD434 pKa = 4.03NNSGAQGSGGAKK446 pKa = 8.64TATGSVTVNISAVNDD461 pKa = 3.61APVAGSQSNSTAEE474 pKa = 3.83DD475 pKa = 3.82APFNGQIIASDD486 pKa = 3.49VDD488 pKa = 4.09GGSLTYY494 pKa = 10.66SLQTGASNGSVTLNSSTGAFVYY516 pKa = 9.72TPNAGYY522 pKa = 10.4SGADD526 pKa = 3.01SFTVQVSDD534 pKa = 3.6GRR536 pKa = 11.84GGFVNSVINMSVTPVDD552 pKa = 3.92DD553 pKa = 4.85APVLSLTTPATPVEE567 pKa = 4.4GNAAEE572 pKa = 4.57GNLISTASATDD583 pKa = 3.32EE584 pKa = 4.17DD585 pKa = 5.11TPASGLNFSLTSNPGNVYY603 pKa = 10.46AINAATGAVTLTAAGAALVNAGGDD627 pKa = 3.75LPPVEE632 pKa = 4.42VTVTDD637 pKa = 4.25GNLTDD642 pKa = 3.83SGSMNVPATQDD653 pKa = 3.15INDD656 pKa = 4.24EE657 pKa = 4.09PTAIPLSLTTVEE669 pKa = 5.36DD670 pKa = 3.46NAIDD674 pKa = 4.08GKK676 pKa = 11.07VVASDD681 pKa = 4.05PDD683 pKa = 4.33GDD685 pKa = 3.95TLTYY689 pKa = 10.64SITTGNGPQHH699 pKa = 5.79GTVTLNPNGTFSYY712 pKa = 10.82LPTKK716 pKa = 10.37DD717 pKa = 3.83YY718 pKa = 11.62NGTDD722 pKa = 3.32AFTVTIDD729 pKa = 3.97DD730 pKa = 4.22GNGGVTISLVNITILPFNDD749 pKa = 3.47APEE752 pKa = 4.17TANQSKK758 pKa = 6.84TTNEE762 pKa = 4.04DD763 pKa = 3.05QSVSGQVIASDD774 pKa = 3.52VDD776 pKa = 3.57GDD778 pKa = 3.82RR779 pKa = 11.84LSYY782 pKa = 10.38IIQSGVANGSLLLNTATGEE801 pKa = 4.07YY802 pKa = 9.58TYY804 pKa = 10.82TPNKK808 pKa = 9.86DD809 pKa = 3.6YY810 pKa = 11.54NGTDD814 pKa = 3.12SFTLRR819 pKa = 11.84VYY821 pKa = 10.4DD822 pKa = 3.74PKK824 pKa = 11.11GGYY827 pKa = 7.4TEE829 pKa = 4.3SVVTITVAPVNDD841 pKa = 4.4DD842 pKa = 3.94PKK844 pKa = 10.96SSPLFVTTVEE854 pKa = 4.74DD855 pKa = 3.43NTATGKK861 pKa = 10.42INARR865 pKa = 11.84DD866 pKa = 3.51VDD868 pKa = 4.36GDD870 pKa = 3.81TLTYY874 pKa = 10.64SIATGNGPQHH884 pKa = 5.81GTVTLNPDD892 pKa = 3.2GTFSYY897 pKa = 10.84LPAKK901 pKa = 10.18DD902 pKa = 4.14YY903 pKa = 11.64NGTDD907 pKa = 3.22AFTVTIDD914 pKa = 3.97DD915 pKa = 4.26GNGGVTTSFVNITIQPFNDD934 pKa = 3.45APEE937 pKa = 4.16TANQSKK943 pKa = 6.84TTNEE947 pKa = 4.04DD948 pKa = 3.06QSVSGQIIASDD959 pKa = 3.51VDD961 pKa = 3.77GDD963 pKa = 3.76RR964 pKa = 11.84LNYY967 pKa = 9.84VIQSGVAHH975 pKa = 6.83GSILLNTVTGEE986 pKa = 3.92YY987 pKa = 9.84TYY989 pKa = 10.93TPNKK993 pKa = 9.91DD994 pKa = 3.55YY995 pKa = 11.56NGTDD999 pKa = 2.89TFTIRR1004 pKa = 11.84VYY1006 pKa = 10.63DD1007 pKa = 3.88PKK1009 pKa = 11.01GGYY1012 pKa = 7.53TDD1014 pKa = 3.85SVVTVTVAPVNDD1026 pKa = 4.33APTSGPLFMNTVEE1039 pKa = 5.54DD1040 pKa = 3.49IAANGTIITRR1050 pKa = 11.84DD1051 pKa = 3.13VDD1053 pKa = 4.03GGTLTYY1059 pKa = 10.67SIATGNGPQHH1069 pKa = 5.81GTVTLNPDD1077 pKa = 3.2GTFSYY1082 pKa = 10.84LPAKK1086 pKa = 10.18DD1087 pKa = 4.14YY1088 pKa = 11.64NGTDD1092 pKa = 3.22AFTVTIDD1099 pKa = 3.97DD1100 pKa = 4.26GNGGVTTSLVNITIQPFNDD1119 pKa = 3.45APEE1122 pKa = 4.16TANQSKK1128 pKa = 6.84TTNEE1132 pKa = 4.04DD1133 pKa = 3.06QSVSGQIIASDD1144 pKa = 3.5VDD1146 pKa = 3.95GDD1148 pKa = 4.17SLSYY1152 pKa = 10.57AIQSGATHH1160 pKa = 6.82GSILLYY1166 pKa = 9.86TATGEE1171 pKa = 3.93YY1172 pKa = 9.82TYY1174 pKa = 10.91RR1175 pKa = 11.84PNKK1178 pKa = 10.01DD1179 pKa = 3.37YY1180 pKa = 11.62NGTDD1184 pKa = 2.89TFTIRR1189 pKa = 11.84VYY1191 pKa = 10.63DD1192 pKa = 3.88PKK1194 pKa = 11.01GGYY1197 pKa = 7.53TDD1199 pKa = 3.85SVVTVTVNPVDD1210 pKa = 3.98DD1211 pKa = 5.08APILSLTTPTTPVEE1225 pKa = 4.22GSAAEE1230 pKa = 4.25GNLISTASATDD1241 pKa = 3.29KK1242 pKa = 9.68DD1243 pKa = 4.3TPATGLSFSLTNNPGNVYY1261 pKa = 10.38AINAATGAVTLTAAGAALVNAGGDD1285 pKa = 3.75LPPVEE1290 pKa = 4.47VTVTDD1295 pKa = 3.62GTTPISGSVNVPATSDD1311 pKa = 3.34INDD1314 pKa = 3.45APIINVTAQTLIEE1327 pKa = 4.11NSAAAGSVAATYY1339 pKa = 7.68TASDD1343 pKa = 3.91EE1344 pKa = 4.31EE1345 pKa = 4.26ASSFIVSFTPGSNDD1359 pKa = 3.04DD1360 pKa = 3.81GYY1362 pKa = 11.89YY1363 pKa = 10.32VLANGRR1369 pKa = 11.84VEE1371 pKa = 4.1LTQDD1375 pKa = 3.41GADD1378 pKa = 3.55HH1379 pKa = 6.51VNAGNSLPPVSLVVTEE1395 pKa = 4.41TGSPALTGTDD1405 pKa = 3.02SDD1407 pKa = 4.43TPNVTLTNDD1416 pKa = 3.32APVANADD1423 pKa = 3.65TASTNEE1429 pKa = 4.09EE1430 pKa = 4.24TPVTIDD1436 pKa = 3.38VLSNDD1441 pKa = 3.83SDD1443 pKa = 3.93VDD1445 pKa = 4.02GPTLSIKK1452 pKa = 10.34SASVDD1457 pKa = 3.53PAQGTVSIVAGQLVFTPASNFTGAATITYY1486 pKa = 9.59VSTDD1490 pKa = 3.23GSDD1493 pKa = 3.7DD1494 pKa = 3.72SAPATVTVTVNPVNDD1509 pKa = 3.63APVANTDD1516 pKa = 3.57TASTNEE1522 pKa = 4.19DD1523 pKa = 3.71TPVTIDD1529 pKa = 3.49VLSNDD1534 pKa = 3.7SDD1536 pKa = 3.85VDD1538 pKa = 4.24GPALSIKK1545 pKa = 10.11SASVDD1550 pKa = 3.53PAQGTVSIVAGQLVFTPASNFTGTATITYY1579 pKa = 9.37VSTDD1583 pKa = 3.23GSDD1586 pKa = 3.7DD1587 pKa = 3.72SAPATVTVTVNPINDD1602 pKa = 3.59APVAQASTASTGEE1615 pKa = 3.71NTAFNGNVPAASDD1628 pKa = 3.0IDD1630 pKa = 3.96GTVNPNGYY1638 pKa = 10.5ALVNGPAAGSLTFNANGSYY1657 pKa = 10.66SFNPDD1662 pKa = 2.77SAFDD1666 pKa = 3.99DD1667 pKa = 4.19LAAGEE1672 pKa = 4.41SRR1674 pKa = 11.84NVTFTYY1680 pKa = 9.74TATDD1684 pKa = 3.4NNN1686 pKa = 4.08

Molecular weight: 170.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A2ITS0|A0A2A2ITS0_9PSED Cell shape-determining protein MreC OS=Pseudomonas sp. HAR-UPW-AIA-41 OX=1985301 GN=CK486_11480 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTLKK11 pKa = 10.31RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3419

0

3419

1101474

33

4341

322.2

35.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.225 ± 0.052

1.042 ± 0.014

5.182 ± 0.029

6.097 ± 0.038

3.513 ± 0.025

7.843 ± 0.047

2.234 ± 0.021

4.52 ± 0.036

3.286 ± 0.038

12.536 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.199 ± 0.022

2.861 ± 0.028

4.842 ± 0.032

5.089 ± 0.039

6.652 ± 0.048

5.709 ± 0.034

4.4 ± 0.037

6.821 ± 0.038

1.45 ± 0.02

2.5 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski