Pacific flying fox faeces associated circular DNA virus-12

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140CTV5|A0A140CTV5_9VIRU Putative capsid protein OS=Pacific flying fox faeces associated circular DNA virus-12 OX=1796007 PE=4 SV=1
MM1 pKa = 7.95QYY3 pKa = 9.94MDD5 pKa = 4.98DD6 pKa = 3.64AAADD10 pKa = 3.64VEE12 pKa = 4.45RR13 pKa = 11.84PDD15 pKa = 4.51IIAAQFRR22 pKa = 11.84LSAKK26 pKa = 10.18NLFLTYY32 pKa = 7.88PHH34 pKa = 7.0CGVQLEE40 pKa = 4.2IARR43 pKa = 11.84DD44 pKa = 3.73IILEE48 pKa = 3.9ALGRR52 pKa = 11.84GNVEE56 pKa = 4.08YY57 pKa = 10.12MVVAHH62 pKa = 6.55EE63 pKa = 4.08RR64 pKa = 11.84HH65 pKa = 5.6QDD67 pKa = 3.23GDD69 pKa = 3.87LHH71 pKa = 7.49IHH73 pKa = 6.39AVVCLIVRR81 pKa = 11.84CDD83 pKa = 3.13IRR85 pKa = 11.84NANLLDD91 pKa = 3.91LGEE94 pKa = 4.12YY95 pKa = 9.24HH96 pKa = 7.26GNYY99 pKa = 8.68QAIRR103 pKa = 11.84NVRR106 pKa = 11.84ASVRR110 pKa = 11.84YY111 pKa = 8.74VKK113 pKa = 10.38KK114 pKa = 10.51DD115 pKa = 3.32NVFIEE120 pKa = 4.34YY121 pKa = 8.75GTINAKK127 pKa = 10.27YY128 pKa = 10.64ADD130 pKa = 4.17AEE132 pKa = 4.1DD133 pKa = 3.92RR134 pKa = 11.84AAVLAKK140 pKa = 10.32LVQCTSEE147 pKa = 3.84ADD149 pKa = 3.26AMHH152 pKa = 7.05FIYY155 pKa = 11.11VNGHH159 pKa = 5.02ARR161 pKa = 11.84EE162 pKa = 4.33SYY164 pKa = 11.18LLLQYY169 pKa = 9.98WKK171 pKa = 8.97TKK173 pKa = 9.77QGEE176 pKa = 4.61SKK178 pKa = 10.38PGARR182 pKa = 11.84FARR185 pKa = 11.84DD186 pKa = 3.05AFLPCASLLAALEE199 pKa = 4.03EE200 pKa = 4.43LEE202 pKa = 4.44EE203 pKa = 4.5SDD205 pKa = 5.06RR206 pKa = 11.84CLLLIGPSGWGKK218 pKa = 6.87TQYY221 pKa = 11.48LLAHH225 pKa = 5.6YY226 pKa = 9.51NEE228 pKa = 4.26RR229 pKa = 11.84QIVRR233 pKa = 11.84ATHH236 pKa = 6.14MDD238 pKa = 3.75DD239 pKa = 3.61LKK241 pKa = 11.11KK242 pKa = 10.35ISKK245 pKa = 6.0TTEE248 pKa = 3.32ILILDD253 pKa = 4.3DD254 pKa = 4.54LSYY257 pKa = 11.5GHH259 pKa = 7.78LPRR262 pKa = 11.84QTLLHH267 pKa = 6.41LFDD270 pKa = 4.24MKK272 pKa = 10.37TDD274 pKa = 3.43RR275 pKa = 11.84SVHH278 pKa = 4.36VRR280 pKa = 11.84YY281 pKa = 7.65TVANLHH287 pKa = 5.82QSLLVILVGNEE298 pKa = 3.72LEE300 pKa = 4.5LVLGAHH306 pKa = 6.62IEE308 pKa = 4.29DD309 pKa = 3.82VAVMRR314 pKa = 11.84RR315 pKa = 11.84VTVFQLPHH323 pKa = 6.76RR324 pKa = 11.84LWDD327 pKa = 3.48

Molecular weight: 37.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140CTV5|A0A140CTV5_9VIRU Putative capsid protein OS=Pacific flying fox faeces associated circular DNA virus-12 OX=1796007 PE=4 SV=1
MM1 pKa = 7.95PIRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84ALTSSRR14 pKa = 11.84RR15 pKa = 11.84VRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84TQRR22 pKa = 11.84RR23 pKa = 11.84GGRR26 pKa = 11.84GRR28 pKa = 11.84GLRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84SFRR36 pKa = 11.84RR37 pKa = 11.84GIRR40 pKa = 11.84RR41 pKa = 11.84GRR43 pKa = 11.84RR44 pKa = 11.84GGFRR48 pKa = 11.84SRR50 pKa = 11.84RR51 pKa = 11.84NTGLSYY57 pKa = 10.92SVNITGWTSALLSWRR72 pKa = 11.84QSDD75 pKa = 3.76DD76 pKa = 2.9ACYY79 pKa = 10.72DD80 pKa = 3.6FLRR83 pKa = 11.84TEE85 pKa = 4.24HH86 pKa = 6.96RR87 pKa = 11.84PFINPEE93 pKa = 4.33LIGDD97 pKa = 4.07SNLEE101 pKa = 3.99VMGVANTTASQPAIPTKK118 pKa = 10.25SLVMGWRR125 pKa = 11.84DD126 pKa = 3.31QMSQFGRR133 pKa = 11.84NYY135 pKa = 8.86RR136 pKa = 11.84WGYY139 pKa = 9.1LKK141 pKa = 10.31SVRR144 pKa = 11.84YY145 pKa = 9.69RR146 pKa = 11.84IEE148 pKa = 3.3WCGTVTEE155 pKa = 4.61ATSYY159 pKa = 10.95MNGFMGWNASDD170 pKa = 3.87PGNVQGNQQGTIVTPRR186 pKa = 11.84WDD188 pKa = 3.55RR189 pKa = 11.84PFVNLLFSKK198 pKa = 10.06VHH200 pKa = 6.36EE201 pKa = 4.45EE202 pKa = 3.6FDD204 pKa = 3.39YY205 pKa = 11.3HH206 pKa = 7.5RR207 pKa = 11.84NQTGTAQLEE216 pKa = 4.3PFQNLGLGQPTRR228 pKa = 11.84LNYY231 pKa = 9.53PDD233 pKa = 3.7TMSSFQYY240 pKa = 8.03TTGGQVGSMMWSQLLNNRR258 pKa = 11.84AIKK261 pKa = 10.21KK262 pKa = 9.99LRR264 pKa = 11.84FTPRR268 pKa = 11.84DD269 pKa = 3.2NVKK272 pKa = 8.49WVKK275 pKa = 8.55WVPMTRR281 pKa = 11.84ADD283 pKa = 3.64KK284 pKa = 10.54MYY286 pKa = 11.09VKK288 pKa = 10.87YY289 pKa = 10.49DD290 pKa = 3.77LQKK293 pKa = 10.25MLQQTATPSPQYY305 pKa = 10.28YY306 pKa = 10.82DD307 pKa = 2.53MDD309 pKa = 3.93MRR311 pKa = 11.84VGGCWMAQEE320 pKa = 4.52RR321 pKa = 11.84PMAGAQVLGQTVDD334 pKa = 3.36VSISSNDD341 pKa = 3.21LFRR344 pKa = 11.84ITVYY348 pKa = 11.01SDD350 pKa = 2.35WKK352 pKa = 10.56FFGRR356 pKa = 11.84MQCFNTPLAA365 pKa = 4.57

Molecular weight: 42.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

692

327

365

346.0

39.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.081 ± 1.962

1.445 ± 0.254

5.78 ± 0.817

4.191 ± 1.255

3.468 ± 0.666

6.936 ± 1.332

2.746 ± 1.399

4.335 ± 0.961

3.468 ± 0.331

9.682 ± 2.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.468 ± 0.865

4.48 ± 0.528

3.468 ± 0.865

5.202 ± 0.799

9.971 ± 1.914

5.491 ± 1.187

5.636 ± 1.281

6.792 ± 0.755

2.168 ± 0.815

4.191 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski