Escherichia phage KBNP1711

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Kuravirus; Escherichia virus Septima11

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 126 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6AS96|W6AS96_9CAUD Uncharacterized protein OS=Escherichia phage KBNP1711 OX=1436889 GN=ECBP3_0064 PE=4 SV=1
MM1 pKa = 7.58IFNASGNMPSLLWGIEE17 pKa = 4.19TNVLEE22 pKa = 4.43EE23 pKa = 4.0GQYY26 pKa = 10.57IDD28 pKa = 5.45FDD30 pKa = 4.09QLLEE34 pKa = 4.06LQKK37 pKa = 10.88VVNQLVSDD45 pKa = 3.84EE46 pKa = 4.12QSRR49 pKa = 11.84LLQEE53 pKa = 4.27SLSGDD58 pKa = 4.35FVCDD62 pKa = 3.0GCTII66 pKa = 3.95

Molecular weight: 7.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6AS10|W6AS10_9CAUD Uncharacterized protein OS=Escherichia phage KBNP1711 OX=1436889 GN=ECBP3_0110 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.17ARR4 pKa = 11.84RR5 pKa = 11.84TIWTPMQIEE14 pKa = 4.14NLRR17 pKa = 11.84RR18 pKa = 11.84LIKK21 pKa = 9.88EE22 pKa = 3.75YY23 pKa = 10.69SVQEE27 pKa = 3.87VAQIMNVTEE36 pKa = 4.05NAVNTACTKK45 pKa = 10.48FGISRR50 pKa = 11.84AVIRR54 pKa = 11.84VKK56 pKa = 9.81WSKK59 pKa = 11.29EE60 pKa = 3.16EE61 pKa = 3.89DD62 pKa = 3.11QFLIRR67 pKa = 11.84NAGFMSIQRR76 pKa = 11.84MADD79 pKa = 3.16MLGRR83 pKa = 11.84SYY85 pKa = 11.54AAVQNRR91 pKa = 11.84ACMHH95 pKa = 6.36LNISLKK101 pKa = 10.81LKK103 pKa = 10.59

Molecular weight: 11.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

126

0

126

22573

38

1473

179.2

20.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.921 ± 0.521

1.245 ± 0.158

6.282 ± 0.242

6.933 ± 0.316

3.956 ± 0.159

7.075 ± 0.346

1.723 ± 0.155

5.622 ± 0.231

6.211 ± 0.311

7.327 ± 0.187

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.747 ± 0.149

5.6 ± 0.262

3.681 ± 0.157

4.049 ± 0.317

5.201 ± 0.216

6.769 ± 0.239

5.768 ± 0.276

6.964 ± 0.238

1.44 ± 0.104

3.486 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski