Streptococcus phage P7574

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286QPQ7|A0A286QPQ7_9CAUD Uncharacterized protein OS=Streptococcus phage P7574 OX=1971430 GN=P7574_32 PE=4 SV=1
MM1 pKa = 7.3SVSKK5 pKa = 9.08EE6 pKa = 4.26TIMQTLNLDD15 pKa = 3.8EE16 pKa = 4.97TDD18 pKa = 3.78DD19 pKa = 3.8TALIPAYY26 pKa = 9.65IEE28 pKa = 3.9SARR31 pKa = 11.84QYY33 pKa = 10.3VVNSVGDD40 pKa = 3.57DD41 pKa = 3.42PKK43 pKa = 11.06FYY45 pKa = 11.02DD46 pKa = 4.08LDD48 pKa = 3.81SVRR51 pKa = 11.84ALFDD55 pKa = 3.34TAVIALTSSYY65 pKa = 8.66FTYY68 pKa = 10.07RR69 pKa = 11.84VALTDD74 pKa = 3.37TATYY78 pKa = 9.41PVNLTLNSIIGQLRR92 pKa = 11.84GLYY95 pKa = 8.4ATYY98 pKa = 10.89SEE100 pKa = 4.8VVANGG105 pKa = 3.05

Molecular weight: 11.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286QPU3|A0A286QPU3_9CAUD HNH endonuclease OS=Streptococcus phage P7574 OX=1971430 GN=P7574_50 PE=4 SV=1
MM1 pKa = 7.62HH2 pKa = 7.43KK3 pKa = 9.84PSKK6 pKa = 10.64YY7 pKa = 9.16FIKK10 pKa = 10.42RR11 pKa = 11.84RR12 pKa = 11.84IQFYY16 pKa = 10.9SSLALLVARR25 pKa = 11.84LEE27 pKa = 4.21FKK29 pKa = 10.73IKK31 pKa = 10.61GVVNKK36 pKa = 10.18KK37 pKa = 9.45SPRR40 pKa = 11.84QSLPRR45 pKa = 11.84TADD48 pKa = 3.45NIIIPP53 pKa = 4.12

Molecular weight: 6.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

10888

35

1602

222.2

25.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.475 ± 0.467

0.615 ± 0.131

6.383 ± 0.256

6.503 ± 0.377

4.335 ± 0.18

6.585 ± 0.497

1.451 ± 0.128

6.457 ± 0.219

8.413 ± 0.517

8.174 ± 0.407

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.526 ± 0.138

6.172 ± 0.255

2.838 ± 0.195

4.243 ± 0.176

4.243 ± 0.264

6.888 ± 0.414

6.126 ± 0.312

6.08 ± 0.201

1.451 ± 0.139

4.041 ± 0.304
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski