Amino acid dipepetide frequency for Pedobacter sp. HMWF019

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.184AlaAla: 6.184 ± 0.075
0.595AlaCys: 0.595 ± 0.02
4.03AlaAsp: 4.03 ± 0.051
4.298AlaGlu: 4.298 ± 0.049
3.402AlaPhe: 3.402 ± 0.044
5.532AlaGly: 5.532 ± 0.065
1.137AlaHis: 1.137 ± 0.026
4.905AlaIle: 4.905 ± 0.056
4.499AlaLys: 4.499 ± 0.05
6.908AlaLeu: 6.908 ± 0.067
1.588AlaMet: 1.588 ± 0.031
3.602AlaAsn: 3.602 ± 0.049
2.27AlaPro: 2.27 ± 0.039
2.927AlaGln: 2.927 ± 0.045
2.416AlaArg: 2.416 ± 0.038
4.804AlaSer: 4.804 ± 0.055
3.862AlaThr: 3.862 ± 0.052
4.732AlaVal: 4.732 ± 0.054
0.805AlaTrp: 0.805 ± 0.021
3.082AlaTyr: 3.082 ± 0.037
0.001AlaXaa: 0.001 ± 0.001
Cys
0.473CysAla: 0.473 ± 0.019
0.115CysCys: 0.115 ± 0.008
0.334CysAsp: 0.334 ± 0.014
0.32CysGlu: 0.32 ± 0.013
0.426CysPhe: 0.426 ± 0.015
0.559CysGly: 0.559 ± 0.018
0.16CysHis: 0.16 ± 0.009
0.593CysIle: 0.593 ± 0.019
0.546CysLys: 0.546 ± 0.017
0.757CysLeu: 0.757 ± 0.021
0.169CysMet: 0.169 ± 0.011
0.352CysAsn: 0.352 ± 0.015
0.265CysPro: 0.265 ± 0.012
0.199CysGln: 0.199 ± 0.009
0.303CysArg: 0.303 ± 0.014
0.522CysSer: 0.522 ± 0.017
0.423CysThr: 0.423 ± 0.014
0.405CysVal: 0.405 ± 0.012
0.082CysTrp: 0.082 ± 0.006
0.28CysTyr: 0.28 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
3.763AspAla: 3.763 ± 0.049
0.354AspCys: 0.354 ± 0.012
2.171AspAsp: 2.171 ± 0.039
2.861AspGlu: 2.861 ± 0.046
3.083AspPhe: 3.083 ± 0.039
3.784AspGly: 3.784 ± 0.048
1.146AspHis: 1.146 ± 0.023
3.73AspIle: 3.73 ± 0.044
3.633AspLys: 3.633 ± 0.051
5.375AspLeu: 5.375 ± 0.056
1.125AspMet: 1.125 ± 0.023
2.524AspAsn: 2.524 ± 0.04
2.299AspPro: 2.299 ± 0.037
2.411AspGln: 2.411 ± 0.036
2.147AspArg: 2.147 ± 0.031
2.578AspSer: 2.578 ± 0.039
2.327AspThr: 2.327 ± 0.039
3.326AspVal: 3.326 ± 0.041
0.789AspTrp: 0.789 ± 0.023
2.574AspTyr: 2.574 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
4.013GluAla: 4.013 ± 0.048
0.298GluCys: 0.298 ± 0.012
2.682GluAsp: 2.682 ± 0.038
3.778GluGlu: 3.778 ± 0.051
2.299GluPhe: 2.299 ± 0.032
3.455GluGly: 3.455 ± 0.044
1.121GluHis: 1.121 ± 0.025
4.387GluIle: 4.387 ± 0.049
4.711GluLys: 4.711 ± 0.05
5.711GluLeu: 5.711 ± 0.065
1.419GluMet: 1.419 ± 0.026
3.333GluAsn: 3.333 ± 0.044
1.532GluPro: 1.532 ± 0.031
2.602GluGln: 2.602 ± 0.038
2.465GluArg: 2.465 ± 0.032
2.863GluSer: 2.863 ± 0.036
2.769GluThr: 2.769 ± 0.035
3.701GluVal: 3.701 ± 0.045
0.611GluTrp: 0.611 ± 0.019
1.968GluTyr: 1.968 ± 0.033
0.0GluXaa: 0.0 ± 0.0
Phe
3.071PheAla: 3.071 ± 0.041
0.468PheCys: 0.468 ± 0.016
2.789PheAsp: 2.789 ± 0.037
2.689PheGlu: 2.689 ± 0.039
2.449PhePhe: 2.449 ± 0.041
3.346PheGly: 3.346 ± 0.042
0.785PheHis: 0.785 ± 0.019
3.435PheIle: 3.435 ± 0.052
3.532PheLys: 3.532 ± 0.039
4.5PheLeu: 4.5 ± 0.052
1.119PheMet: 1.119 ± 0.022
3.168PheAsn: 3.168 ± 0.046
1.763PhePro: 1.763 ± 0.03
1.46PheGln: 1.46 ± 0.027
1.793PheArg: 1.793 ± 0.028
4.002PheSer: 4.002 ± 0.044
3.018PheThr: 3.018 ± 0.048
2.76PheVal: 2.76 ± 0.04
0.581PheTrp: 0.581 ± 0.017
2.222PheTyr: 2.222 ± 0.037
0.0PheXaa: 0.0 ± 0.0
Gly
4.72GlyAla: 4.72 ± 0.061
0.556GlyCys: 0.556 ± 0.017
3.307GlyAsp: 3.307 ± 0.048
3.27GlyGlu: 3.27 ± 0.049
3.627GlyPhe: 3.627 ± 0.042
4.894GlyGly: 4.894 ± 0.06
1.193GlyHis: 1.193 ± 0.025
5.353GlyIle: 5.353 ± 0.065
5.468GlyLys: 5.468 ± 0.059
6.732GlyLeu: 6.732 ± 0.069
1.614GlyMet: 1.614 ± 0.031
3.917GlyAsn: 3.917 ± 0.046
1.689GlyPro: 1.689 ± 0.033
2.52GlyGln: 2.52 ± 0.037
2.596GlyArg: 2.596 ± 0.047
4.706GlySer: 4.706 ± 0.059
4.494GlyThr: 4.494 ± 0.092
4.376GlyVal: 4.376 ± 0.055
0.973GlyTrp: 0.973 ± 0.023
3.312GlyTyr: 3.312 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
1.106HisAla: 1.106 ± 0.022
0.163HisCys: 0.163 ± 0.008
0.761HisAsp: 0.761 ± 0.018
0.926HisGlu: 0.926 ± 0.025
1.148HisPhe: 1.148 ± 0.027
1.07HisGly: 1.07 ± 0.024
0.491HisHis: 0.491 ± 0.018
1.269HisIle: 1.269 ± 0.022
1.068HisLys: 1.068 ± 0.024
1.964HisLeu: 1.964 ± 0.038
0.381HisMet: 0.381 ± 0.015
0.836HisAsn: 0.836 ± 0.019
0.959HisPro: 0.959 ± 0.023
0.83HisGln: 0.83 ± 0.022
0.699HisArg: 0.699 ± 0.019
1.047HisSer: 1.047 ± 0.022
0.949HisThr: 0.949 ± 0.021
0.925HisVal: 0.925 ± 0.021
0.236HisTrp: 0.236 ± 0.011
0.85HisTyr: 0.85 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
5.412IleAla: 5.412 ± 0.056
0.656IleCys: 0.656 ± 0.02
3.999IleAsp: 3.999 ± 0.05
4.102IleGlu: 4.102 ± 0.051
3.042IlePhe: 3.042 ± 0.041
4.904IleGly: 4.904 ± 0.063
1.268IleHis: 1.268 ± 0.029
4.455IleIle: 4.455 ± 0.055
4.966IleLys: 4.966 ± 0.057
6.526IleLeu: 6.526 ± 0.07
1.301IleMet: 1.301 ± 0.026
4.196IleAsn: 4.196 ± 0.047
3.072IlePro: 3.072 ± 0.04
2.468IleGln: 2.468 ± 0.036
3.092IleArg: 3.092 ± 0.041
5.522IleSer: 5.522 ± 0.052
4.312IleThr: 4.312 ± 0.05
3.964IleVal: 3.964 ± 0.044
0.757IleTrp: 0.757 ± 0.021
2.772IleTyr: 2.772 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
5.034LysAla: 5.034 ± 0.056
0.295LysCys: 0.295 ± 0.013
4.432LysAsp: 4.432 ± 0.048
4.918LysGlu: 4.918 ± 0.05
2.693LysPhe: 2.693 ± 0.037
4.784LysGly: 4.784 ± 0.05
1.239LysHis: 1.239 ± 0.027
5.256LysIle: 5.256 ± 0.059
5.663LysLys: 5.663 ± 0.062
6.216LysLeu: 6.216 ± 0.06
1.831LysMet: 1.831 ± 0.03
4.453LysAsn: 4.453 ± 0.047
2.673LysPro: 2.673 ± 0.039
2.796LysGln: 2.796 ± 0.039
2.839LysArg: 2.839 ± 0.041
4.233LysSer: 4.233 ± 0.048
4.428LysThr: 4.428 ± 0.056
4.511LysVal: 4.511 ± 0.055
0.826LysTrp: 0.826 ± 0.018
2.993LysTyr: 2.993 ± 0.037
0.0LysXaa: 0.0 ± 0.0
Leu
6.494LeuAla: 6.494 ± 0.066
0.806LeuCys: 0.806 ± 0.02
4.83LeuAsp: 4.83 ± 0.056
5.083LeuGlu: 5.083 ± 0.061
4.828LeuPhe: 4.828 ± 0.059
5.858LeuGly: 5.858 ± 0.065
1.721LeuHis: 1.721 ± 0.032
6.586LeuIle: 6.586 ± 0.076
7.929LeuLys: 7.929 ± 0.06
9.765LeuLeu: 9.765 ± 0.097
2.226LeuMet: 2.226 ± 0.03
6.158LeuAsn: 6.158 ± 0.051
4.2LeuPro: 4.2 ± 0.047
3.847LeuGln: 3.847 ± 0.051
3.754LeuArg: 3.754 ± 0.039
7.648LeuSer: 7.648 ± 0.07
5.527LeuThr: 5.527 ± 0.06
5.475LeuVal: 5.475 ± 0.055
1.043LeuTrp: 1.043 ± 0.024
3.499LeuTyr: 3.499 ± 0.039
0.0LeuXaa: 0.0 ± 0.0
Met
1.716MetAla: 1.716 ± 0.031
0.128MetCys: 0.128 ± 0.009
1.214MetAsp: 1.214 ± 0.023
1.401MetGlu: 1.401 ± 0.03
0.768MetPhe: 0.768 ± 0.018
1.475MetGly: 1.475 ± 0.026
0.396MetHis: 0.396 ± 0.015
1.581MetIle: 1.581 ± 0.031
1.99MetLys: 1.99 ± 0.03
2.097MetLeu: 2.097 ± 0.03
0.627MetMet: 0.627 ± 0.02
1.346MetAsn: 1.346 ± 0.026
1.013MetPro: 1.013 ± 0.023
0.852MetGln: 0.852 ± 0.021
0.93MetArg: 0.93 ± 0.02
1.334MetSer: 1.334 ± 0.029
1.101MetThr: 1.101 ± 0.029
1.452MetVal: 1.452 ± 0.025
0.17MetTrp: 0.17 ± 0.009
0.662MetTyr: 0.662 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.972AsnAla: 3.972 ± 0.05
0.371AsnCys: 0.371 ± 0.015
2.696AsnAsp: 2.696 ± 0.033
2.92AsnGlu: 2.92 ± 0.038
2.757AsnPhe: 2.757 ± 0.038
4.424AsnGly: 4.424 ± 0.078
0.965AsnHis: 0.965 ± 0.022
4.148AsnIle: 4.148 ± 0.055
3.808AsnLys: 3.808 ± 0.048
5.47AsnLeu: 5.47 ± 0.054
1.2AsnMet: 1.2 ± 0.022
3.3AsnAsn: 3.3 ± 0.056
2.812AsnPro: 2.812 ± 0.043
2.178AsnGln: 2.178 ± 0.038
2.361AsnArg: 2.361 ± 0.036
3.69AsnSer: 3.69 ± 0.049
3.574AsnThr: 3.574 ± 0.047
3.229AsnVal: 3.229 ± 0.045
0.828AsnTrp: 0.828 ± 0.02
2.905AsnTyr: 2.905 ± 0.044
0.0AsnXaa: 0.0 ± 0.0
Pro
3.068ProAla: 3.068 ± 0.043
0.225ProCys: 0.225 ± 0.011
2.494ProAsp: 2.494 ± 0.039
2.846ProGlu: 2.846 ± 0.04
1.946ProPhe: 1.946 ± 0.034
3.058ProGly: 3.058 ± 0.044
0.586ProHis: 0.586 ± 0.017
2.195ProIle: 2.195 ± 0.035
2.225ProLys: 2.225 ± 0.034
3.51ProLeu: 3.51 ± 0.043
0.768ProMet: 0.768 ± 0.02
1.977ProAsn: 1.977 ± 0.034
0.967ProPro: 0.967 ± 0.024
1.408ProGln: 1.408 ± 0.035
1.09ProArg: 1.09 ± 0.025
2.539ProSer: 2.539 ± 0.033
1.821ProThr: 1.821 ± 0.033
3.308ProVal: 3.308 ± 0.04
0.422ProTrp: 0.422 ± 0.014
1.619ProTyr: 1.619 ± 0.028
0.0ProXaa: 0.0 ± 0.001
Gln
2.631GlnAla: 2.631 ± 0.04
0.181GlnCys: 0.181 ± 0.009
1.781GlnAsp: 1.781 ± 0.031
2.192GlnGlu: 2.192 ± 0.035
1.698GlnPhe: 1.698 ± 0.03
2.256GlnGly: 2.256 ± 0.042
0.758GlnHis: 0.758 ± 0.023
2.764GlnIle: 2.764 ± 0.04
2.754GlnLys: 2.754 ± 0.041
3.966GlnLeu: 3.966 ± 0.053
0.908GlnMet: 0.908 ± 0.024
2.324GlnAsn: 2.324 ± 0.04
1.436GlnPro: 1.436 ± 0.027
2.079GlnGln: 2.079 ± 0.037
1.511GlnArg: 1.511 ± 0.025
2.384GlnSer: 2.384 ± 0.029
2.288GlnThr: 2.288 ± 0.032
2.44GlnVal: 2.44 ± 0.038
0.454GlnTrp: 0.454 ± 0.014
1.627GlnTyr: 1.627 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
2.446ArgAla: 2.446 ± 0.041
0.227ArgCys: 0.227 ± 0.011
1.829ArgAsp: 1.829 ± 0.026
2.154ArgGlu: 2.154 ± 0.037
2.123ArgPhe: 2.123 ± 0.036
2.154ArgGly: 2.154 ± 0.036
0.61ArgHis: 0.61 ± 0.017
3.201ArgIle: 3.201 ± 0.043
2.942ArgLys: 2.942 ± 0.035
3.911ArgLeu: 3.911 ± 0.044
0.979ArgMet: 0.979 ± 0.022
2.331ArgAsn: 2.331 ± 0.036
1.408ArgPro: 1.408 ± 0.031
1.365ArgGln: 1.365 ± 0.026
1.519ArgArg: 1.519 ± 0.029
2.505ArgSer: 2.505 ± 0.041
2.106ArgThr: 2.106 ± 0.029
2.347ArgVal: 2.347 ± 0.033
0.545ArgTrp: 0.545 ± 0.014
1.931ArgTyr: 1.931 ± 0.033
0.0ArgXaa: 0.0 ± 0.0
Ser
4.874SerAla: 4.874 ± 0.059
0.57SerCys: 0.57 ± 0.018
3.296SerAsp: 3.296 ± 0.044
3.039SerGlu: 3.039 ± 0.038
3.801SerPhe: 3.801 ± 0.047
5.39SerGly: 5.39 ± 0.053
1.051SerHis: 1.051 ± 0.024
4.981SerIle: 4.981 ± 0.05
4.421SerLys: 4.421 ± 0.045
6.68SerLeu: 6.68 ± 0.064
1.378SerMet: 1.378 ± 0.027
3.588SerAsn: 3.588 ± 0.049
2.518SerPro: 2.518 ± 0.036
2.036SerGln: 2.036 ± 0.033
2.592SerArg: 2.592 ± 0.034
4.758SerSer: 4.758 ± 0.062
3.852SerThr: 3.852 ± 0.05
4.364SerVal: 4.364 ± 0.054
0.873SerTrp: 0.873 ± 0.02
3.114SerTyr: 3.114 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
4.577ThrAla: 4.577 ± 0.054
0.349ThrCys: 0.349 ± 0.016
3.378ThrAsp: 3.378 ± 0.044
3.033ThrGlu: 3.033 ± 0.04
2.721ThrPhe: 2.721 ± 0.04
5.037ThrGly: 5.037 ± 0.067
0.932ThrHis: 0.932 ± 0.022
3.996ThrIle: 3.996 ± 0.047
3.355ThrLys: 3.355 ± 0.044
5.503ThrLeu: 5.503 ± 0.053
0.995ThrMet: 0.995 ± 0.022
2.942ThrAsn: 2.942 ± 0.054
2.478ThrPro: 2.478 ± 0.036
1.887ThrGln: 1.887 ± 0.028
1.935ThrArg: 1.935 ± 0.034
3.798ThrSer: 3.798 ± 0.049
3.598ThrThr: 3.598 ± 0.069
3.82ThrVal: 3.82 ± 0.051
0.653ThrTrp: 0.653 ± 0.022
2.573ThrTyr: 2.573 ± 0.049
0.002ThrXaa: 0.002 ± 0.001
Val
4.248ValAla: 4.248 ± 0.051
0.501ValCys: 0.501 ± 0.017
3.058ValAsp: 3.058 ± 0.043
3.224ValGlu: 3.224 ± 0.039
3.202ValPhe: 3.202 ± 0.042
3.493ValGly: 3.493 ± 0.05
1.043ValHis: 1.043 ± 0.02
4.527ValIle: 4.527 ± 0.055
4.708ValLys: 4.708 ± 0.057
6.349ValLeu: 6.349 ± 0.066
1.467ValMet: 1.467 ± 0.029
3.773ValAsn: 3.773 ± 0.048
2.535ValPro: 2.535 ± 0.037
2.218ValGln: 2.218 ± 0.034
2.246ValArg: 2.246 ± 0.033
4.587ValSer: 4.587 ± 0.053
3.62ValThr: 3.62 ± 0.052
4.197ValVal: 4.197 ± 0.051
0.69ValTrp: 0.69 ± 0.019
2.627ValTyr: 2.627 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
0.788TrpAla: 0.788 ± 0.019
0.116TrpCys: 0.116 ± 0.007
0.606TrpAsp: 0.606 ± 0.017
0.694TrpGlu: 0.694 ± 0.019
0.602TrpPhe: 0.602 ± 0.018
0.845TrpGly: 0.845 ± 0.023
0.21TrpHis: 0.21 ± 0.011
0.792TrpIle: 0.792 ± 0.022
0.938TrpLys: 0.938 ± 0.021
1.208TrpLeu: 1.208 ± 0.024
0.352TrpMet: 0.352 ± 0.014
0.725TrpAsn: 0.725 ± 0.02
0.381TrpPro: 0.381 ± 0.015
0.485TrpGln: 0.485 ± 0.015
0.504TrpArg: 0.504 ± 0.017
0.734TrpSer: 0.734 ± 0.021
0.674TrpThr: 0.674 ± 0.021
0.696TrpVal: 0.696 ± 0.021
0.184TrpTrp: 0.184 ± 0.011
0.531TrpTyr: 0.531 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.989TyrAla: 2.989 ± 0.035
0.31TyrCys: 0.31 ± 0.012
2.312TyrAsp: 2.312 ± 0.04
1.971TyrGlu: 1.971 ± 0.029
2.336TyrPhe: 2.336 ± 0.042
3.0TyrGly: 3.0 ± 0.045
0.865TyrHis: 0.865 ± 0.02
2.631TyrIle: 2.631 ± 0.036
2.956TyrLys: 2.956 ± 0.033
4.129TyrLeu: 4.129 ± 0.051
0.808TyrMet: 0.808 ± 0.021
2.765TyrAsn: 2.765 ± 0.05
1.802TyrPro: 1.802 ± 0.027
1.86TyrGln: 1.86 ± 0.029
1.84TyrArg: 1.84 ± 0.032
2.921TyrSer: 2.921 ± 0.045
2.819TyrThr: 2.819 ± 0.039
2.285TyrVal: 2.285 ± 0.033
0.543TyrTrp: 0.543 ± 0.017
2.02TyrTyr: 2.02 ± 0.039
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.001
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.001XaaLys: 0.001 ± 0.001
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.008XaaXaa: 0.008 ± 0.003
Statistics based on 5695 proteins (2068325 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski