Anaerotruncus sp. 22A2-44

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Anaerotruncus; unclassified Anaerotruncus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2733 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A498CRV6|A0A498CRV6_9FIRM Uncharacterized protein OS=Anaerotruncus sp. 22A2-44 OX=2321404 GN=D4A47_05095 PE=4 SV=1
MM1 pKa = 7.38NGKK4 pKa = 10.05KK5 pKa = 9.0MLALLLAVVMAVMPLSGCSMKK26 pKa = 10.71GAAEE30 pKa = 3.94QPAAPSAAPADD41 pKa = 4.05EE42 pKa = 4.47VPADD46 pKa = 3.6KK47 pKa = 10.91TEE49 pKa = 3.85AVAPGEE55 pKa = 4.06EE56 pKa = 4.14KK57 pKa = 10.22QLKK60 pKa = 9.81IGFSIITLTFPYY72 pKa = 9.05YY73 pKa = 11.41VKK75 pKa = 10.41MLDD78 pKa = 3.71GFEE81 pKa = 4.33AACKK85 pKa = 9.42EE86 pKa = 4.17KK87 pKa = 10.55GWDD90 pKa = 3.6YY91 pKa = 11.44VYY93 pKa = 10.64TDD95 pKa = 3.49AGMDD99 pKa = 3.52VEE101 pKa = 4.9KK102 pKa = 10.19TVNDD106 pKa = 4.21CLDD109 pKa = 3.83LTLKK113 pKa = 10.91DD114 pKa = 3.05IDD116 pKa = 4.21ALVIASWYY124 pKa = 10.23GDD126 pKa = 3.39SLQDD130 pKa = 4.41VFEE133 pKa = 4.09QCKK136 pKa = 9.67AANIPVFLIDD146 pKa = 4.15TGSLPPDD153 pKa = 3.62GDD155 pKa = 3.95YY156 pKa = 9.98VTNIGTVDD164 pKa = 4.21FDD166 pKa = 3.43AGYY169 pKa = 11.07NGGYY173 pKa = 8.16WAAGQMKK180 pKa = 9.62EE181 pKa = 3.96AGKK184 pKa = 9.83EE185 pKa = 3.83AVRR188 pKa = 11.84MISFTTATSNGRR200 pKa = 11.84NRR202 pKa = 11.84ADD204 pKa = 3.41GFVKK208 pKa = 10.48GLEE211 pKa = 4.19EE212 pKa = 4.61GGLTVEE218 pKa = 4.89VLNEE222 pKa = 3.74YY223 pKa = 10.63LGDD226 pKa = 3.77SRR228 pKa = 11.84EE229 pKa = 4.35SYY231 pKa = 7.54MTSCEE236 pKa = 4.22DD237 pKa = 3.68ALVTYY242 pKa = 10.14PEE244 pKa = 4.95LDD246 pKa = 3.43LVFAANAQGGLGAYY260 pKa = 7.62DD261 pKa = 3.28ACVAANRR268 pKa = 11.84PEE270 pKa = 4.01VKK272 pKa = 9.96IVGFDD277 pKa = 3.72CEE279 pKa = 4.34DD280 pKa = 3.83EE281 pKa = 4.15EE282 pKa = 5.95VEE284 pKa = 5.47LIDD287 pKa = 5.99KK288 pKa = 7.51GTQYY292 pKa = 10.39IASVMQLPAGMAQQTIQNIEE312 pKa = 3.96DD313 pKa = 3.69YY314 pKa = 9.83LYY316 pKa = 11.32NGATFEE322 pKa = 4.23KK323 pKa = 9.23STPYY327 pKa = 10.68EE328 pKa = 4.02SGVYY332 pKa = 9.18CAQGALTTEE341 pKa = 5.3DD342 pKa = 3.29ILGSKK347 pKa = 9.55

Molecular weight: 37.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A498CKL6|A0A498CKL6_9FIRM Stage 0 sporulation protein A homolog OS=Anaerotruncus sp. 22A2-44 OX=2321404 GN=D4A47_11370 PE=4 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84RR3 pKa = 11.84KK4 pKa = 9.44QPPLLPPLRR13 pKa = 11.84TPPRR17 pKa = 11.84TPPAPPAARR26 pKa = 11.84AGPARR31 pKa = 11.84IRR33 pKa = 11.84PAQAGGPRR41 pKa = 11.84TVPPRR46 pKa = 11.84GQRR49 pKa = 11.84SFSFRR54 pKa = 11.84FPQSNPII61 pKa = 3.55

Molecular weight: 6.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2733

0

2733

866568

28

3342

317.1

34.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.486 ± 0.069

1.591 ± 0.021

5.606 ± 0.041

6.771 ± 0.043

3.853 ± 0.037

8.698 ± 0.057

1.588 ± 0.019

5.619 ± 0.041

4.368 ± 0.041

9.716 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.695 ± 0.025

3.079 ± 0.031

4.468 ± 0.035

2.743 ± 0.026

6.317 ± 0.057

5.453 ± 0.036

5.196 ± 0.05

7.616 ± 0.038

0.878 ± 0.015

3.261 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski