Salinisphaera hydrothermalis (strain C41B8)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Salinisphaerales; Salinisphaeraceae; Salinisphaera; Salinisphaera hydrothermalis

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3807 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A084ILM5|A0A084ILM5_SALHC Uncharacterized protein OS=Salinisphaera hydrothermalis (strain C41B8) OX=1304275 GN=C41B8_08490 PE=4 SV=1
MM1 pKa = 7.07TFVVTEE7 pKa = 3.83NCIKK11 pKa = 10.53CKK13 pKa = 10.0YY14 pKa = 7.43TDD16 pKa = 3.85CVEE19 pKa = 4.15VCPVDD24 pKa = 4.28CFHH27 pKa = 7.46EE28 pKa = 5.35GPNFLAIDD36 pKa = 3.86PEE38 pKa = 4.21EE39 pKa = 5.95CIDD42 pKa = 3.82CTLCEE47 pKa = 4.24PEE49 pKa = 4.9CPVEE53 pKa = 4.7AIVSEE58 pKa = 4.48DD59 pKa = 5.06DD60 pKa = 4.37LPEE63 pKa = 4.41DD64 pKa = 4.11QADD67 pKa = 4.04FLQLNAEE74 pKa = 4.92LAAEE78 pKa = 4.17WPVITEE84 pKa = 4.04VKK86 pKa = 10.3KK87 pKa = 10.49PPEE90 pKa = 4.75DD91 pKa = 3.67ADD93 pKa = 3.46EE94 pKa = 4.4WADD97 pKa = 3.6KK98 pKa = 10.25PNKK101 pKa = 9.89RR102 pKa = 11.84EE103 pKa = 3.83LLEE106 pKa = 4.03RR107 pKa = 4.55

Molecular weight: 12.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A084IKP6|A0A084IKP6_SALHC Pyruvate dehydrogenase E1 component OS=Salinisphaera hydrothermalis (strain C41B8) OX=1304275 GN=aceE PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.16RR14 pKa = 11.84THH16 pKa = 5.96GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.97NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.86GRR39 pKa = 11.84KK40 pKa = 9.07RR41 pKa = 11.84LTPP44 pKa = 3.95

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3807

0

3807

1233367

17

3217

324.0

35.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.735 ± 0.058

0.884 ± 0.012

6.309 ± 0.037

5.276 ± 0.043

3.438 ± 0.028

8.29 ± 0.035

2.533 ± 0.021

4.999 ± 0.03

2.711 ± 0.041

10.009 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.364 ± 0.02

2.763 ± 0.027

4.921 ± 0.026

3.419 ± 0.027

7.43 ± 0.045

5.338 ± 0.031

5.254 ± 0.025

7.323 ± 0.031

1.442 ± 0.016

2.563 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski