Pseudogymnoascus sp. VKM F-4515 (FW-2607)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus; unclassified Pseudogymnoascus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10675 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A094DA61|A0A094DA61_9PEZI Uncharacterized protein OS=Pseudogymnoascus sp. VKM F-4515 (FW-2607) OX=1420909 GN=V496_04030 PE=4 SV=1
MM1 pKa = 7.54SGYY4 pKa = 11.32GNDD7 pKa = 4.67NNDD10 pKa = 2.89SSNNRR15 pKa = 11.84GGDD18 pKa = 3.46SYY20 pKa = 11.91GSSNNNDD27 pKa = 3.42SYY29 pKa = 11.71GSSGNTRR36 pKa = 11.84SGDD39 pKa = 3.35NDD41 pKa = 3.92SYY43 pKa = 11.69GSSNTRR49 pKa = 11.84SSDD52 pKa = 3.38NNDD55 pKa = 3.12SYY57 pKa = 12.03GSSNKK62 pKa = 9.85SSNDD66 pKa = 2.94NDD68 pKa = 4.34SYY70 pKa = 11.84GSSNKK75 pKa = 9.92DD76 pKa = 2.96SSDD79 pKa = 3.53SYY81 pKa = 11.33GSSNTRR87 pKa = 11.84SNDD90 pKa = 3.11NDD92 pKa = 3.35NDD94 pKa = 3.47NSYY97 pKa = 11.35GSSNKK102 pKa = 9.81SSSNDD107 pKa = 3.0NDD109 pKa = 4.13SYY111 pKa = 11.77GSSNKK116 pKa = 9.08TSSDD120 pKa = 3.45SYY122 pKa = 10.93GSSNTRR128 pKa = 11.84SNDD131 pKa = 3.11NDD133 pKa = 3.35NDD135 pKa = 3.47NSYY138 pKa = 11.35GSSNKK143 pKa = 9.81SSSNDD148 pKa = 2.92NDD150 pKa = 3.18NSYY153 pKa = 11.39GSSNKK158 pKa = 9.86SSNDD162 pKa = 3.1DD163 pKa = 3.56DD164 pKa = 6.0SYY166 pKa = 11.88GSSNKK171 pKa = 9.79SSSNTYY177 pKa = 10.45GSSNDD182 pKa = 3.51NNDD185 pKa = 3.27SYY187 pKa = 11.88GSSNTRR193 pKa = 11.84SNDD196 pKa = 3.11NDD198 pKa = 3.35NDD200 pKa = 3.47NSYY203 pKa = 11.35GSSNKK208 pKa = 9.77SSSDD212 pKa = 3.48SYY214 pKa = 11.64GSSGNNNNDD223 pKa = 3.2SYY225 pKa = 11.68GSSGNTGSSTIDD237 pKa = 3.2KK238 pKa = 9.95LAEE241 pKa = 3.9KK242 pKa = 10.56AGDD245 pKa = 3.57YY246 pKa = 11.22LGGNKK251 pKa = 9.82NSGNDD256 pKa = 3.42YY257 pKa = 11.37

Molecular weight: 26.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A094DR10|A0A094DR10_9PEZI PBP_domain domain-containing protein OS=Pseudogymnoascus sp. VKM F-4515 (FW-2607) OX=1420909 GN=V496_00828 PE=4 SV=1
SS1 pKa = 7.0RR2 pKa = 11.84AQARR6 pKa = 11.84AQAGAQARR14 pKa = 11.84AQAGAQAGAQAGARR28 pKa = 11.84ARR30 pKa = 11.84ARR32 pKa = 11.84ARR34 pKa = 11.84AA35 pKa = 3.41

Molecular weight: 3.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10675

0

10675

5316900

13

5903

498.1

54.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.855 ± 0.025

1.227 ± 0.008

5.63 ± 0.016

6.298 ± 0.024

3.631 ± 0.014

7.203 ± 0.023

2.275 ± 0.01

5.092 ± 0.016

4.973 ± 0.018

8.778 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.154 ± 0.008

3.74 ± 0.012

5.909 ± 0.025

3.843 ± 0.018

5.894 ± 0.02

8.118 ± 0.021

6.068 ± 0.015

6.133 ± 0.016

1.421 ± 0.009

2.758 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski