Staphylococcus piscifermentans

Taxonomy: cellular organisms; Bacteria;

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2389 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A239UBT5|A0A239UBT5_9STAP Cysteine desulfurase OS=Staphylococcus piscifermentans OX=70258 GN=csdB PE=3 SV=1
MM1 pKa = 7.87IDD3 pKa = 3.15MFLYY7 pKa = 10.62DD8 pKa = 4.95DD9 pKa = 4.41NEE11 pKa = 4.32EE12 pKa = 4.71ADD14 pKa = 3.67VQYY17 pKa = 11.41VGMVGEE23 pKa = 4.28EE24 pKa = 3.79SRR26 pKa = 11.84YY27 pKa = 10.6DD28 pKa = 3.6LMMFQTNRR36 pKa = 11.84HH37 pKa = 4.86YY38 pKa = 11.51GKK40 pKa = 10.53VLVLNMQTNKK50 pKa = 10.29FGIIGPDD57 pKa = 3.38DD58 pKa = 4.02LAEE61 pKa = 4.96DD62 pKa = 4.92GYY64 pKa = 10.99VAYY67 pKa = 10.79VLGVSDD73 pKa = 5.39AEE75 pKa = 4.36GEE77 pKa = 4.23EE78 pKa = 3.9LTEE81 pKa = 4.02YY82 pKa = 10.7LKK84 pKa = 11.02DD85 pKa = 3.92VIPSGTFDD93 pKa = 3.17SGEE96 pKa = 4.08YY97 pKa = 10.34

Molecular weight: 10.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A239UL23|A0A239UL23_9STAP Sulfite reductase [NADPH] hemoprotein beta-component OS=Staphylococcus piscifermentans OX=70258 GN=cysI PE=3 SV=1
MM1 pKa = 7.49VKK3 pKa = 9.1RR4 pKa = 11.84TYY6 pKa = 10.35QPNKK10 pKa = 8.16RR11 pKa = 11.84KK12 pKa = 9.54HH13 pKa = 5.99SKK15 pKa = 8.87VHH17 pKa = 5.77GFRR20 pKa = 11.84KK21 pKa = 10.02RR22 pKa = 11.84MSTKK26 pKa = 10.18NGRR29 pKa = 11.84KK30 pKa = 8.49VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2389

0

2389

742465

30

4482

310.8

34.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.079 ± 0.09

0.591 ± 0.015

5.857 ± 0.061

7.093 ± 0.067

4.467 ± 0.047

6.463 ± 0.069

2.309 ± 0.029

8.096 ± 0.063

7.197 ± 0.071

9.242 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.029

5.001 ± 0.041

3.533 ± 0.034

4.029 ± 0.038

3.682 ± 0.042

5.73 ± 0.034

5.547 ± 0.058

6.823 ± 0.044

0.784 ± 0.018

3.785 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski