Halomonas phage QHHSV-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0GUX6|A0A1J0GUX6_9CAUD Uncharacterized protein OS=Halomonas phage QHHSV-1 OX=1913108 GN=QHH_34 PE=4 SV=1
MM1 pKa = 7.16SANRR5 pKa = 11.84QRR7 pKa = 11.84LKK9 pKa = 11.01SAIQRR14 pKa = 11.84IAVEE18 pKa = 4.16APQIRR23 pKa = 11.84DD24 pKa = 3.41LLGKK28 pKa = 10.16GKK30 pKa = 10.32NKK32 pKa = 10.14GGKK35 pKa = 9.48ASSAGVGTSEE45 pKa = 4.52AEE47 pKa = 4.15EE48 pKa = 4.49SRR50 pKa = 11.84LCCDD54 pKa = 3.7GSTSSAANPGTEE66 pKa = 4.16EE67 pKa = 4.44RR68 pKa = 11.84DD69 pKa = 3.63PDD71 pKa = 3.96SSSQDD76 pKa = 3.29GLNSDD81 pKa = 3.82DD82 pKa = 4.11PARR85 pKa = 11.84LEE87 pKa = 4.51SGDD90 pKa = 3.61GTLSGVTDD98 pKa = 4.33CATGEE103 pKa = 4.21QVCFDD108 pKa = 3.79GAGFIPPEE116 pKa = 4.34GWDD119 pKa = 4.32DD120 pKa = 3.89PQSPPIDD127 pKa = 3.47PTYY130 pKa = 11.02TEE132 pKa = 3.89GKK134 pKa = 8.8YY135 pKa = 8.68WRR137 pKa = 11.84ADD139 pKa = 3.42DD140 pKa = 5.18GGGSDD145 pKa = 4.03VGFGATKK152 pKa = 10.0QAAIDD157 pKa = 3.58ATSFARR163 pKa = 11.84SDD165 pKa = 4.0CVQCSNGAEE174 pKa = 4.29LIGRR178 pKa = 11.84PLTANGQTSCPGTTILCDD196 pKa = 3.66DD197 pKa = 4.5GGYY200 pKa = 10.83DD201 pKa = 4.03GFDD204 pKa = 3.48AYY206 pKa = 11.37SVTQGDD212 pKa = 4.42CTGSHH217 pKa = 6.71AEE219 pKa = 4.09EE220 pKa = 5.32SYY222 pKa = 11.3CNPSEE227 pKa = 4.13GDD229 pKa = 3.42ILSTDD234 pKa = 3.13TWPSDD239 pKa = 2.85GCTNLIIKK247 pKa = 10.2DD248 pKa = 3.65GAIVGSKK255 pKa = 10.33YY256 pKa = 10.74DD257 pKa = 3.67PEE259 pKa = 5.34NDD261 pKa = 3.45GSYY264 pKa = 10.42SAPRR268 pKa = 11.84DD269 pKa = 4.06SIDD272 pKa = 4.03LCDD275 pKa = 4.32PEE277 pKa = 5.47NGARR281 pKa = 11.84ITLRR285 pKa = 11.84ASGQSDD291 pKa = 3.68WKK293 pKa = 9.97TIDD296 pKa = 4.51AINGGDD302 pKa = 3.59GYY304 pKa = 10.72LYY306 pKa = 10.98SGGKK310 pKa = 9.43QIARR314 pKa = 11.84VSGDD318 pKa = 3.27EE319 pKa = 4.0FTDD322 pKa = 3.93PNVV325 pKa = 3.07

Molecular weight: 33.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0GUV9|A0A1J0GUV9_9CAUD Uncharacterized protein OS=Halomonas phage QHHSV-1 OX=1913108 GN=QHH_12 PE=4 SV=1
MM1 pKa = 7.15TRR3 pKa = 11.84DD4 pKa = 3.07ASIYY8 pKa = 9.95RR9 pKa = 11.84VEE11 pKa = 4.71DD12 pKa = 3.47PATGEE17 pKa = 4.08MVTLKK22 pKa = 10.42EE23 pKa = 3.65LASRR27 pKa = 11.84HH28 pKa = 5.94RR29 pKa = 11.84IPYY32 pKa = 6.86PTVQRR37 pKa = 11.84RR38 pKa = 11.84NRR40 pKa = 11.84EE41 pKa = 4.08GKK43 pKa = 8.36TGAALVKK50 pKa = 10.6KK51 pKa = 9.81PDD53 pKa = 3.13PRR55 pKa = 11.84YY56 pKa = 8.9QVGGRR61 pKa = 11.84RR62 pKa = 11.84SKK64 pKa = 9.92TGGIRR69 pKa = 11.84QPAPSRR75 pKa = 11.84QQVAQEE81 pKa = 4.17VLSTPDD87 pKa = 2.83GRR89 pKa = 11.84LASTLFRR96 pKa = 11.84NYY98 pKa = 9.99GKK100 pKa = 10.28SAA102 pKa = 3.19

Molecular weight: 11.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

10845

30

662

157.2

17.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.876 ± 0.482

0.996 ± 0.155

6.528 ± 0.278

7.22 ± 0.242

2.49 ± 0.188

8.05 ± 0.3

2.241 ± 0.189

4.444 ± 0.18

3.107 ± 0.214

8.373 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.407 ± 0.175

2.416 ± 0.148

4.804 ± 0.271

4.435 ± 0.243

7.994 ± 0.382

5.754 ± 0.34

6.372 ± 0.308

6.279 ± 0.279

1.964 ± 0.155

2.25 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski