Nocardia brasiliensis ATCC 700358

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Nocardia; Nocardia brasiliensis

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8414 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0FA18|K0FA18_9NOCA Uncharacterized protein OS=Nocardia brasiliensis ATCC 700358 OX=1133849 GN=O3I_041500 PE=4 SV=1
MM1 pKa = 7.09NRR3 pKa = 11.84STKK6 pKa = 10.26LVAVVACTAAIVAASVTSAQADD28 pKa = 3.86PEE30 pKa = 4.29PAGIQYY36 pKa = 7.86TAQAVGSSMTLTTDD50 pKa = 3.56AGSLDD55 pKa = 3.8TADD58 pKa = 4.49DD59 pKa = 3.77RR60 pKa = 11.84LEE62 pKa = 4.2IRR64 pKa = 11.84DD65 pKa = 3.53NDD67 pKa = 4.26GNLMAAFPLGYY78 pKa = 10.39YY79 pKa = 9.68RR80 pKa = 11.84DD81 pKa = 3.76NQRR84 pKa = 11.84YY85 pKa = 8.49PIAAVIDD92 pKa = 4.35GNTATLTPSVDD103 pKa = 3.33PATATPAPGTTDD115 pKa = 3.19QLHH118 pKa = 7.01DD119 pKa = 3.79VALDD123 pKa = 3.54PTSPAFGAALSTFSTVVGIGTALGTIIGTLVGASLGCLLGGLLVGGAATLPTFGALTIPGFLGGCIVTAIPGAALGAIVGTIAFGVPAIIIGGILLAGTLGAMM226 pKa = 4.63

Molecular weight: 22.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0ESI4|K0ESI4_9NOCA Hydroxypyruvate isomerase OS=Nocardia brasiliensis ATCC 700358 OX=1133849 GN=O3I_010465 PE=3 SV=1
MM1 pKa = 7.46GVPGVAGTRR10 pKa = 11.84PKK12 pKa = 10.68RR13 pKa = 11.84LVLRR17 pKa = 11.84PKK19 pKa = 10.53RR20 pKa = 11.84RR21 pKa = 11.84VTPLTRR27 pKa = 11.84LVSRR31 pKa = 11.84LTGQVSGPTRR41 pKa = 11.84LVWRR45 pKa = 11.84PSRR48 pKa = 11.84LVSRR52 pKa = 11.84PTRR55 pKa = 11.84QVSRR59 pKa = 11.84LTCLVWRR66 pKa = 11.84PSRR69 pKa = 11.84LVSRR73 pKa = 11.84PTRR76 pKa = 11.84QVSRR80 pKa = 11.84LTRR83 pKa = 11.84LAWRR87 pKa = 11.84PNHH90 pKa = 6.19LVSRR94 pKa = 11.84PPGQLSS100 pKa = 3.16

Molecular weight: 11.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8414

0

8414

2750564

30

15203

326.9

35.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.725 ± 0.037

0.765 ± 0.009

6.083 ± 0.024

5.302 ± 0.024

3.031 ± 0.015

8.793 ± 0.027

2.231 ± 0.013

4.077 ± 0.018

1.91 ± 0.021

10.37 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.815 ± 0.012

1.986 ± 0.015

5.922 ± 0.027

2.986 ± 0.014

7.681 ± 0.027

5.021 ± 0.018

6.225 ± 0.021

8.459 ± 0.03

1.495 ± 0.01

2.122 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski