Pigeonpea sterility mosaic emaravirus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Fimoviridae; Emaravirus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G4DI13|A0A0G4DI13_9VIRU p5 protein OS=Pigeonpea sterility mosaic emaravirus 2 OX=1980430 GN=p5 PE=4 SV=1
MM1 pKa = 7.54ASKK4 pKa = 10.67GFEE7 pKa = 3.76RR8 pKa = 11.84TFNICNTANLEE19 pKa = 4.08EE20 pKa = 5.13LYY22 pKa = 10.91SEE24 pKa = 5.47LEE26 pKa = 3.94SQLYY30 pKa = 9.65YY31 pKa = 10.89SAIEE35 pKa = 3.98YY36 pKa = 8.54MLSIDD41 pKa = 4.5LYY43 pKa = 11.47GEE45 pKa = 3.82IAVLRR50 pKa = 11.84EE51 pKa = 3.76IYY53 pKa = 10.25EE54 pKa = 4.44DD55 pKa = 3.54NSLDD59 pKa = 3.75LSILISSAPYY69 pKa = 10.38LIIEE73 pKa = 4.55GNSVSRR79 pKa = 11.84KK80 pKa = 6.33SVKK83 pKa = 10.3YY84 pKa = 6.24FTRR87 pKa = 11.84YY88 pKa = 9.46LQFSMSLFGVSEE100 pKa = 4.27DD101 pKa = 3.97ACSGLCPDD109 pKa = 3.27YY110 pKa = 11.05HH111 pKa = 8.48IFGSVHH117 pKa = 5.46RR118 pKa = 11.84QKK120 pKa = 10.89QILPYY125 pKa = 9.99IPNNSKK131 pKa = 10.69YY132 pKa = 10.73LLVDD136 pKa = 3.11TSILKK141 pKa = 10.09EE142 pKa = 4.33SVWDD146 pKa = 3.5VMRR149 pKa = 11.84GVDD152 pKa = 3.64PYY154 pKa = 11.34KK155 pKa = 10.5VLEE158 pKa = 4.16RR159 pKa = 11.84VYY161 pKa = 10.85LDD163 pKa = 3.33QPRR166 pKa = 11.84SMDD169 pKa = 3.47LSEE172 pKa = 4.21STVAFNFHH180 pKa = 6.14IMAISYY186 pKa = 10.22ISIRR190 pKa = 11.84SSSKK194 pKa = 10.96LKK196 pKa = 10.5IEE198 pKa = 4.22CKK200 pKa = 10.09KK201 pKa = 10.61LRR203 pKa = 11.84LSSRR207 pKa = 11.84IFSDD211 pKa = 3.05NGATSASHH219 pKa = 6.75VKK221 pKa = 10.31DD222 pKa = 3.82AEE224 pKa = 4.22FNSIDD229 pKa = 3.89KK230 pKa = 10.68SHH232 pKa = 6.81SPSQLEE238 pKa = 3.9

Molecular weight: 27.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G4DD52|A0A0G4DD52_9VIRU Movment protein OS=Pigeonpea sterility mosaic emaravirus 2 OX=1980430 GN=putative movement protein PE=4 SV=1
MM1 pKa = 7.45PPKK4 pKa = 10.23RR5 pKa = 11.84SISSTSAPKK14 pKa = 10.06AQPGALQEE22 pKa = 3.97NSILLGSGSKK32 pKa = 10.52LRR34 pKa = 11.84VIKK37 pKa = 9.15LTNVVNGKK45 pKa = 8.36LTNAEE50 pKa = 4.14SSEE53 pKa = 4.15LEE55 pKa = 4.59PIDD58 pKa = 4.89VEE60 pKa = 4.35VSPFTSTSQNVSNFTLHH77 pKa = 6.94NYY79 pKa = 9.59RR80 pKa = 11.84SCCHH84 pKa = 6.39VDD86 pKa = 3.49TVAAHH91 pKa = 6.49LSKK94 pKa = 10.94SKK96 pKa = 10.35EE97 pKa = 3.98IKK99 pKa = 10.24EE100 pKa = 4.08KK101 pKa = 10.49LQSRR105 pKa = 11.84NLKK108 pKa = 10.18LATSKK113 pKa = 10.92DD114 pKa = 3.37DD115 pKa = 3.57HH116 pKa = 7.16LVVVKK121 pKa = 10.64DD122 pKa = 3.93MNDD125 pKa = 3.05STVINVVSFNKK136 pKa = 10.12ACAIMAAGILKK147 pKa = 8.91HH148 pKa = 5.84TFDD151 pKa = 5.8EE152 pKa = 4.45EE153 pKa = 4.4FDD155 pKa = 3.74WKK157 pKa = 8.97TGKK160 pKa = 9.66YY161 pKa = 10.17VKK163 pKa = 9.57TDD165 pKa = 2.99NKK167 pKa = 9.98TKK169 pKa = 10.43VVPNPAIINRR179 pKa = 11.84LAGQMGLSAGNPYY192 pKa = 10.72YY193 pKa = 10.44WMIVPGYY200 pKa = 8.6EE201 pKa = 4.21FLYY204 pKa = 10.04EE205 pKa = 4.26LYY207 pKa = 9.64PAEE210 pKa = 4.09VLAYY214 pKa = 9.16TLVRR218 pKa = 11.84LQFRR222 pKa = 11.84KK223 pKa = 10.2NLNIPDD229 pKa = 4.13KK230 pKa = 9.49MTDD233 pKa = 3.1ADD235 pKa = 3.86IVSSLVMKK243 pKa = 9.43MNRR246 pKa = 11.84IHH248 pKa = 7.37KK249 pKa = 10.08LEE251 pKa = 3.91QTSFDD256 pKa = 4.03DD257 pKa = 5.13AINTIGRR264 pKa = 11.84DD265 pKa = 3.81NVSEE269 pKa = 4.56AYY271 pKa = 10.51VEE273 pKa = 3.99LARR276 pKa = 11.84DD277 pKa = 3.33IGSTSKK283 pKa = 9.71TKK285 pKa = 10.82RR286 pKa = 11.84NDD288 pKa = 2.92EE289 pKa = 4.45AIIKK293 pKa = 9.57FKK295 pKa = 10.93EE296 pKa = 4.42LISTFLPALEE306 pKa = 4.26ADD308 pKa = 4.18RR309 pKa = 11.84LASLL313 pKa = 4.46

Molecular weight: 34.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4328

238

2294

721.3

83.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.043 ± 0.59

1.941 ± 0.398

6.285 ± 0.409

5.799 ± 0.417

4.321 ± 0.209

3.581 ± 0.387

2.518 ± 0.285

9.219 ± 0.381

7.971 ± 0.234

9.011 ± 0.737

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.286

7.093 ± 0.468

3.189 ± 0.181

2.518 ± 0.33

3.697 ± 0.207

8.272 ± 0.741

5.869 ± 0.333

5.73 ± 0.774

0.439 ± 0.128

5.869 ± 0.489
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski