Nonomuraea sp. KC333

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae; Nonomuraea; unclassified Nonomuraea

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W2DN82|A0A2W2DN82_9ACTN SAM-dependent methyltransferase OS=Nonomuraea sp. KC333 OX=2070368 GN=C1J01_33725 PE=4 SV=1
MM1 pKa = 7.61EE2 pKa = 5.13ARR4 pKa = 11.84LYY6 pKa = 10.59TRR8 pKa = 11.84TNPDD12 pKa = 2.73TGQIEE17 pKa = 4.45IKK19 pKa = 10.53HH20 pKa = 5.58FEE22 pKa = 4.14IDD24 pKa = 3.6SKK26 pKa = 11.52AQADD30 pKa = 4.02GFGPVWMTHH39 pKa = 6.14FYY41 pKa = 9.77GTDD44 pKa = 3.07APADD48 pKa = 4.13FFASDD53 pKa = 3.48APDD56 pKa = 3.65RR57 pKa = 11.84YY58 pKa = 10.92ADD60 pKa = 3.89ADD62 pKa = 3.68ALEE65 pKa = 5.2IGDD68 pKa = 3.79TCYY71 pKa = 10.66IPDD74 pKa = 4.46LDD76 pKa = 5.27GSLEE80 pKa = 4.46DD81 pKa = 4.13YY82 pKa = 10.89LPSLRR87 pKa = 11.84KK88 pKa = 9.68EE89 pKa = 3.79LDD91 pKa = 3.16AEE93 pKa = 4.42GWILLSSPGTRR104 pKa = 11.84YY105 pKa = 9.29IARR108 pKa = 11.84ITDD111 pKa = 3.16EE112 pKa = 4.64GTWHH116 pKa = 6.25TVTGEE121 pKa = 4.23LVTGTHH127 pKa = 6.1LVVIAEE133 pKa = 4.42AAPTCDD139 pKa = 3.41EE140 pKa = 5.14DD141 pKa = 3.95GTPEE145 pKa = 4.24LEE147 pKa = 4.03PTNRR151 pKa = 11.84VAAVIDD157 pKa = 3.59TGVPADD163 pKa = 3.89EE164 pKa = 4.55PAPYY168 pKa = 10.42VADD171 pKa = 5.22LLDD174 pKa = 3.34QHH176 pKa = 6.24GWLVVGDD183 pKa = 4.12GADD186 pKa = 4.05GYY188 pKa = 11.55DD189 pKa = 3.69DD190 pKa = 4.92AADD193 pKa = 4.05DD194 pKa = 4.83EE195 pKa = 4.77YY196 pKa = 11.68HH197 pKa = 6.71EE198 pKa = 4.53VTPTDD203 pKa = 3.46PTTT206 pKa = 3.27

Molecular weight: 22.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W2DVX7|A0A2W2DVX7_9ACTN Short-chain dehydrogenase OS=Nonomuraea sp. KC333 OX=2070368 GN=C1J01_43595 PE=4 SV=1
MM1 pKa = 7.76PPRR4 pKa = 11.84RR5 pKa = 11.84SPTRR9 pKa = 11.84RR10 pKa = 11.84TPTGHH15 pKa = 6.47RR16 pKa = 11.84PLRR19 pKa = 11.84RR20 pKa = 11.84SPRR23 pKa = 11.84CRR25 pKa = 11.84RR26 pKa = 11.84PPPRR30 pKa = 11.84RR31 pKa = 11.84PARR34 pKa = 11.84PRR36 pKa = 11.84PARR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84PARR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84AGRR50 pKa = 11.84GLIAHH55 pKa = 7.31RR56 pKa = 11.84RR57 pKa = 11.84PACRR61 pKa = 11.84RR62 pKa = 11.84PAPPRR67 pKa = 11.84PAGRR71 pKa = 11.84RR72 pKa = 11.84PARR75 pKa = 11.84RR76 pKa = 3.33

Molecular weight: 9.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9267

0

9267

2822224

29

2227

304.5

32.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.372 ± 0.038

0.763 ± 0.007

5.814 ± 0.024

5.774 ± 0.025

2.84 ± 0.014

9.29 ± 0.025

2.252 ± 0.014

3.476 ± 0.017

1.931 ± 0.019

10.645 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.926 ± 0.011

1.712 ± 0.014

6.046 ± 0.021

2.641 ± 0.014

8.352 ± 0.029

4.842 ± 0.017

5.704 ± 0.02

8.896 ± 0.025

1.58 ± 0.012

2.144 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski