Lake Sinai virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Magsaviricetes; Nodamuvirales; Sinhaliviridae; Sinaivirus

Average proteome isoelectric point is 7.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223DNF2|A0A223DNF2_9VIRU Capsid protein OS=Lake Sinai virus 2 OX=1041831 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 10.52VVFLVSFAVFLICQVPPVSYY22 pKa = 9.91WLEE25 pKa = 4.26DD26 pKa = 3.85LLDD29 pKa = 4.76EE30 pKa = 4.59YY31 pKa = 11.57NTMCSYY37 pKa = 10.91EE38 pKa = 4.02YY39 pKa = 10.97AAAEE43 pKa = 4.11AYY45 pKa = 10.86NNFVHH50 pKa = 6.58KK51 pKa = 10.34HH52 pKa = 4.47RR53 pKa = 11.84VAAYY57 pKa = 8.32AAGVRR62 pKa = 11.84ILRR65 pKa = 11.84YY66 pKa = 7.27RR67 pKa = 11.84TPWYY71 pKa = 10.01CFASRR76 pKa = 11.84SVPVVHH82 pKa = 6.76PFSWLMGQYY91 pKa = 10.43DD92 pKa = 3.72ATIDD96 pKa = 3.47RR97 pKa = 11.84VGTLMQRR104 pKa = 11.84LEE106 pKa = 4.02IANVSISDD114 pKa = 4.68GIRR117 pKa = 11.84TVADD121 pKa = 3.65TALKK125 pKa = 10.44AYY127 pKa = 9.85FYY129 pKa = 10.87YY130 pKa = 10.4QIIWFQLFSIIMLVGFFSAVICSSRR155 pKa = 11.84VRR157 pKa = 11.84VVRR160 pKa = 11.84IRR162 pKa = 11.84NRR164 pKa = 11.84VRR166 pKa = 11.84GYY168 pKa = 10.63SVTEE172 pKa = 3.71LRR174 pKa = 11.84SDD176 pKa = 3.94FEE178 pKa = 4.49EE179 pKa = 4.56SMSAILAPLSRR190 pKa = 11.84GHH192 pKa = 6.3SCLNFQRR199 pKa = 11.84RR200 pKa = 11.84VVEE203 pKa = 4.36SWAIDD208 pKa = 3.18QLLRR212 pKa = 11.84YY213 pKa = 7.7FRR215 pKa = 11.84CFRR218 pKa = 11.84SVASSQGRR226 pKa = 11.84WAEE229 pKa = 3.97VGHH232 pKa = 6.34RR233 pKa = 11.84LHH235 pKa = 6.79RR236 pKa = 11.84CSPVVLDD243 pKa = 3.81GAFVPEE249 pKa = 3.7YY250 pKa = 10.1DD251 pKa = 4.07QRR253 pKa = 11.84FSACRR258 pKa = 11.84RR259 pKa = 11.84HH260 pKa = 6.64PSVCPDD266 pKa = 3.32RR267 pKa = 11.84FDD269 pKa = 3.29IPAAILSHH277 pKa = 6.53VDD279 pKa = 3.35YY280 pKa = 11.57YY281 pKa = 8.69MTPDD285 pKa = 3.22QLAAAVTGPTFIVNHH300 pKa = 7.26DD301 pKa = 3.8YY302 pKa = 11.66SSIDD306 pKa = 3.43TLSVAEE312 pKa = 4.05VSLRR316 pKa = 11.84SAGGLVTASVRR327 pKa = 11.84DD328 pKa = 3.95GPTFGPHH335 pKa = 7.18PYY337 pKa = 9.92YY338 pKa = 10.24HH339 pKa = 7.15WSDD342 pKa = 3.34EE343 pKa = 4.3GVVVASSGAFQYY355 pKa = 10.65FRR357 pKa = 11.84IGRR360 pKa = 11.84LFDD363 pKa = 3.18TTLYY367 pKa = 10.83YY368 pKa = 10.92AFPTSGTYY376 pKa = 10.66ARR378 pKa = 11.84DD379 pKa = 3.54DD380 pKa = 3.71PSALRR385 pKa = 11.84RR386 pKa = 11.84STSGDD391 pKa = 2.87LHH393 pKa = 6.92YY394 pKa = 10.88YY395 pKa = 9.82SPHH398 pKa = 5.8EE399 pKa = 4.1KK400 pKa = 10.41RR401 pKa = 11.84FVSYY405 pKa = 8.88TADD408 pKa = 3.26DD409 pKa = 3.35THH411 pKa = 7.43YY412 pKa = 10.49HH413 pKa = 5.12VFGVSVPRR421 pKa = 11.84SLADD425 pKa = 3.09YY426 pKa = 10.3CAATFCRR433 pKa = 11.84AVRR436 pKa = 11.84DD437 pKa = 3.89DD438 pKa = 4.25KK439 pKa = 11.41FYY441 pKa = 11.4DD442 pKa = 3.6SLRR445 pKa = 11.84SYY447 pKa = 9.17YY448 pKa = 9.02QNRR451 pKa = 11.84CRR453 pKa = 11.84AIGFSDD459 pKa = 4.19ARR461 pKa = 11.84DD462 pKa = 3.46TLMLDD467 pKa = 4.82FIIHH471 pKa = 6.59LCDD474 pKa = 3.9DD475 pKa = 3.77ASLRR479 pKa = 11.84TFGFSRR485 pKa = 11.84LSSAPSSWSAYY496 pKa = 7.55CLSWILVKK504 pKa = 10.52VNHH507 pKa = 5.93MMPLALTSYY516 pKa = 10.11VLNVLHH522 pKa = 7.06RR523 pKa = 11.84FFGAKK528 pKa = 8.97AAPWNWATIHH538 pKa = 6.56LPTYY542 pKa = 11.42DD543 pKa = 3.23MVTSPFRR550 pKa = 11.84LRR552 pKa = 11.84LFGRR556 pKa = 11.84NPTVFNLEE564 pKa = 3.89RR565 pKa = 11.84FRR567 pKa = 11.84AEE569 pKa = 3.62ATVAGSPDD577 pKa = 3.38RR578 pKa = 11.84GQSAEE583 pKa = 4.11GASQDD588 pKa = 3.07HH589 pKa = 6.86HH590 pKa = 7.01EE591 pKa = 4.42YY592 pKa = 10.56DD593 pKa = 4.24IKK595 pKa = 11.45SCDD598 pKa = 3.22ASVASRR604 pKa = 11.84SSSTPLPGDD613 pKa = 3.37SSTSGSVLLLDD624 pKa = 3.87DD625 pKa = 3.41TAGRR629 pKa = 11.84VHH631 pKa = 7.09EE632 pKa = 5.38DD633 pKa = 3.27DD634 pKa = 3.52SSARR638 pKa = 11.84STPHH642 pKa = 6.49RR643 pKa = 11.84GSGILSNKK651 pKa = 9.12SKK653 pKa = 10.8ARR655 pKa = 11.84RR656 pKa = 11.84KK657 pKa = 7.45SHH659 pKa = 6.72RR660 pKa = 11.84PSHH663 pKa = 6.84DD664 pKa = 3.33NSDD667 pKa = 3.59TDD669 pKa = 3.04HH670 pKa = 7.0GYY672 pKa = 10.32SVCHH676 pKa = 6.51PKK678 pKa = 9.4RR679 pKa = 11.84TTHH682 pKa = 6.68PLCPDD687 pKa = 3.63PTASCGPHH695 pKa = 6.48FFMSVCEE702 pKa = 4.39SNEE705 pKa = 4.09SVPTLFHH712 pKa = 5.9AHH714 pKa = 5.59SVGGEE719 pKa = 4.31DD720 pKa = 2.85ITHH723 pKa = 5.71VVDD726 pKa = 3.83PALGASISQRR736 pKa = 11.84FSASQLRR743 pKa = 11.84LLGWSIDD750 pKa = 4.11GIFNTLSGAATSSFVEE766 pKa = 4.32STLLCLSRR774 pKa = 11.84FMRR777 pKa = 11.84EE778 pKa = 4.01VPPTQPVTEE787 pKa = 4.23ARR789 pKa = 11.84RR790 pKa = 11.84SLLFCRR796 pKa = 11.84PRR798 pKa = 11.84EE799 pKa = 3.95RR800 pKa = 11.84FKK802 pKa = 11.25GYY804 pKa = 8.68DD805 pKa = 3.07TFDD808 pKa = 3.03IGFMGIAVPSSKK820 pKa = 9.77TKK822 pKa = 10.8GIEE825 pKa = 3.61ACLRR829 pKa = 11.84EE830 pKa = 4.46VARR833 pKa = 11.84QHH835 pKa = 6.53SRR837 pKa = 11.84ADD839 pKa = 3.7LPNEE843 pKa = 4.11GAA845 pKa = 4.15

Molecular weight: 94.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223DNF3|A0A223DNF3_9VIRU RNA-directed RNA polymerase OS=Lake Sinai virus 2 OX=1041831 PE=4 SV=1
MM1 pKa = 7.46NPPTTTTTTTRR12 pKa = 11.84TIRR15 pKa = 11.84APKK18 pKa = 9.53VQLTPNSATRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84NRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84PAATIAGPLSIQPSSINRR55 pKa = 11.84RR56 pKa = 11.84MVSRR60 pKa = 11.84VTRR63 pKa = 11.84RR64 pKa = 11.84SAVISAAGLAWLRR77 pKa = 11.84QYY79 pKa = 10.78LNPMGPDD86 pKa = 3.32TTSVTGYY93 pKa = 9.4PDD95 pKa = 3.64GSAVTTCIADD105 pKa = 3.35YY106 pKa = 11.33SNTFNVSFPPRR117 pKa = 11.84EE118 pKa = 3.86ALYY121 pKa = 8.77CTGSSSSEE129 pKa = 3.99TPTLVDD135 pKa = 3.27ADD137 pKa = 4.14NYY139 pKa = 11.54AKK141 pKa = 9.73IDD143 pKa = 3.28KK144 pKa = 9.31WSSYY148 pKa = 11.15DD149 pKa = 3.01ITLCVLALPMLRR161 pKa = 11.84NVVMLRR167 pKa = 11.84LYY169 pKa = 9.99PHH171 pKa = 7.0TPTTFALTEE180 pKa = 3.89QTPNFPQRR188 pKa = 11.84FPNWSVYY195 pKa = 10.5SADD198 pKa = 3.01GTRR201 pKa = 11.84FNNGDD206 pKa = 3.53EE207 pKa = 4.19PGYY210 pKa = 9.36LQSYY214 pKa = 9.42VYY216 pKa = 10.53LPNVDD221 pKa = 3.44KK222 pKa = 10.99HH223 pKa = 6.81LSAARR228 pKa = 11.84GYY230 pKa = 10.17RR231 pKa = 11.84LLSRR235 pKa = 11.84GITGIFSAPALEE247 pKa = 4.3TQGFVTACQYY257 pKa = 10.68LAEE260 pKa = 4.9GSIQSQSIKK269 pKa = 10.29SDD271 pKa = 2.81AVRR274 pKa = 11.84SVTVNSDD281 pKa = 3.38GTVKK285 pKa = 10.52NVEE288 pKa = 4.34SSSQTVSSMPRR299 pKa = 11.84YY300 pKa = 9.32VFPLDD305 pKa = 4.14GDD307 pKa = 3.71NCAPSSLTEE316 pKa = 4.38TYY318 pKa = 9.68HH319 pKa = 6.29QAYY322 pKa = 8.98QSKK325 pKa = 8.3ATDD328 pKa = 3.65GFYY331 pKa = 10.3MPMLSSSRR339 pKa = 11.84DD340 pKa = 3.66NPFHH344 pKa = 6.81PPQPRR349 pKa = 11.84AIAVYY354 pKa = 10.4GSFLARR360 pKa = 11.84GCLDD364 pKa = 3.6PVSEE368 pKa = 4.19AHH370 pKa = 6.44EE371 pKa = 4.55ADD373 pKa = 4.37GPTHH377 pKa = 7.48DD378 pKa = 4.07IYY380 pKa = 11.27RR381 pKa = 11.84LNVADD386 pKa = 5.53DD387 pKa = 3.61VAPLFNTGVVWFEE400 pKa = 4.12GISPKK405 pKa = 10.28FSLKK409 pKa = 10.69LKK411 pKa = 9.41TRR413 pKa = 11.84TVLQYY418 pKa = 10.17IPTSGSVLANFTRR431 pKa = 11.84HH432 pKa = 5.55EE433 pKa = 4.0PTYY436 pKa = 11.03DD437 pKa = 4.04QIALDD442 pKa = 3.6AADD445 pKa = 4.17RR446 pKa = 11.84LRR448 pKa = 11.84NLMPHH453 pKa = 7.68AYY455 pKa = 8.52PAAYY459 pKa = 9.97NDD461 pKa = 3.89WGWLGDD467 pKa = 4.31LLDD470 pKa = 4.54SAISMLPGVGTVYY483 pKa = 10.97NIAKK487 pKa = 9.72PLIKK491 pKa = 9.96PAWNWLGNKK500 pKa = 9.42VSDD503 pKa = 3.96FFGNPVARR511 pKa = 11.84DD512 pKa = 3.01GDD514 pKa = 3.68IFFDD518 pKa = 3.96AKK520 pKa = 10.92

Molecular weight: 57.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1987

520

845

662.3

74.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.455 ± 0.288

2.416 ± 0.404

5.788 ± 0.332

3.221 ± 0.279

4.58 ± 0.622

5.033 ± 0.457

2.768 ± 0.565

4.479 ± 0.457

2.315 ± 0.271

9.26 ± 1.297

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.912 ± 0.152

2.969 ± 0.663

6.543 ± 0.667

2.567 ± 0.219

8.052 ± 0.34

10.216 ± 0.708

6.442 ± 0.674

6.794 ± 0.565

1.409 ± 0.051

4.781 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski