Erythrobacter phage vB_EliS_R6L

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8VVJ5|A0A1P8VVJ5_9CAUD Acyl carrier protein OS=Erythrobacter phage vB_EliS_R6L OX=1913119 GN=vBEliSR6L_32 PE=3 SV=1
MM1 pKa = 7.37SAADD5 pKa = 4.05LTPAMRR11 pKa = 11.84QQCAMLCGYY20 pKa = 9.7IKK22 pKa = 9.94NTAQEE27 pKa = 4.17PLAVHH32 pKa = 7.21DD33 pKa = 6.21FDD35 pKa = 7.46DD36 pKa = 4.16DD37 pKa = 3.56WTPAGEE43 pKa = 4.34TYY45 pKa = 10.61RR46 pKa = 11.84DD47 pKa = 3.21WLLQAGYY54 pKa = 10.31IEE56 pKa = 4.98IRR58 pKa = 11.84EE59 pKa = 4.07EE60 pKa = 5.34DD61 pKa = 3.59DD62 pKa = 3.83GQDD65 pKa = 3.05RR66 pKa = 11.84KK67 pKa = 10.95SYY69 pKa = 11.29SDD71 pKa = 3.43DD72 pKa = 3.39QDD74 pKa = 3.86RR75 pKa = 11.84EE76 pKa = 4.66SYY78 pKa = 9.85TSEE81 pKa = 3.86PGGIYY86 pKa = 9.54LTDD89 pKa = 3.57AGKK92 pKa = 10.51AVVNGG97 pKa = 3.78

Molecular weight: 10.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8VVF8|A0A1P8VVF8_9CAUD Uncharacterized protein OS=Erythrobacter phage vB_EliS_R6L OX=1913119 GN=vBEliSR6L_66 PE=4 SV=1
MM1 pKa = 6.8TRR3 pKa = 11.84AIGSHH8 pKa = 6.3HH9 pKa = 6.78SSAAKK14 pKa = 9.78SVVWLTPPGIIEE26 pKa = 4.87SLGGWQSFDD35 pKa = 4.99LDD37 pKa = 3.88PCAAPAPRR45 pKa = 11.84PWSTAQTMNGQADD58 pKa = 4.03ANGLLIDD65 pKa = 3.7WFGRR69 pKa = 11.84VWLNPPYY76 pKa = 10.59SSAEE80 pKa = 3.53IVRR83 pKa = 11.84WLRR86 pKa = 11.84RR87 pKa = 11.84LADD90 pKa = 3.62HH91 pKa = 6.89GRR93 pKa = 11.84GTALIFARR101 pKa = 11.84TEE103 pKa = 3.73TEE105 pKa = 3.68AFRR108 pKa = 11.84RR109 pKa = 11.84EE110 pKa = 3.94VWEE113 pKa = 4.15RR114 pKa = 11.84AHH116 pKa = 7.02GLLFLYY122 pKa = 10.49GRR124 pKa = 11.84LHH126 pKa = 5.61FHH128 pKa = 5.81SAEE131 pKa = 3.55GRR133 pKa = 11.84RR134 pKa = 11.84AVKK137 pKa = 10.63NSGAPSVLCAYY148 pKa = 9.89GQDD151 pKa = 4.0DD152 pKa = 5.24LDD154 pKa = 3.95RR155 pKa = 11.84LAASGIDD162 pKa = 3.58GALVPLRR169 pKa = 11.84LARR172 pKa = 11.84YY173 pKa = 9.91LMIDD177 pKa = 3.91GLTGSWGEE185 pKa = 4.14EE186 pKa = 3.73VARR189 pKa = 11.84WLRR192 pKa = 11.84DD193 pKa = 2.93QGGPVNLSDD202 pKa = 4.61AYY204 pKa = 10.76RR205 pKa = 11.84HH206 pKa = 5.85FARR209 pKa = 11.84HH210 pKa = 5.1PKK212 pKa = 10.35ARR214 pKa = 11.84GKK216 pKa = 9.97RR217 pKa = 11.84HH218 pKa = 4.13WQAKK222 pKa = 8.95IRR224 pKa = 11.84QQLQRR229 pKa = 11.84HH230 pKa = 5.49GRR232 pKa = 11.84RR233 pKa = 11.84IGPGRR238 pKa = 11.84WTGRR242 pKa = 11.84DD243 pKa = 3.13EE244 pKa = 4.12CHH246 pKa = 6.71ARR248 pKa = 11.84LPGLL252 pKa = 3.8

Molecular weight: 28.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

108

0

108

20632

37

1099

191.0

20.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.859 ± 0.518

0.998 ± 0.122

7.202 ± 0.231

6.635 ± 0.318

3.165 ± 0.142

8.414 ± 0.27

1.692 ± 0.148

4.658 ± 0.172

3.63 ± 0.259

7.808 ± 0.22

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.404 ± 0.113

2.869 ± 0.176

5.685 ± 0.274

3.64 ± 0.237

8.167 ± 0.313

4.716 ± 0.252

5.428 ± 0.246

5.991 ± 0.191

1.997 ± 0.166

2.041 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski