Amino acid dipepetide frequency for Erwinia phage Ea35-70

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.566AlaAla: 7.566 ± 0.481
0.692AlaCys: 0.692 ± 0.098
5.155AlaAsp: 5.155 ± 0.259
4.738AlaGlu: 4.738 ± 0.309
3.174AlaPhe: 3.174 ± 0.191
5.692AlaGly: 5.692 ± 0.375
1.551AlaHis: 1.551 ± 0.157
5.275AlaIle: 5.275 ± 0.221
4.797AlaLys: 4.797 ± 0.312
7.494AlaLeu: 7.494 ± 0.3
2.661AlaMet: 2.661 ± 0.168
4.153AlaAsn: 4.153 ± 0.241
2.936AlaPro: 2.936 ± 0.226
3.341AlaGln: 3.341 ± 0.304
3.807AlaArg: 3.807 ± 0.215
4.499AlaSer: 4.499 ± 0.319
4.929AlaThr: 4.929 ± 0.272
6.05AlaVal: 6.05 ± 0.295
1.05AlaTrp: 1.05 ± 0.114
2.673AlaTyr: 2.673 ± 0.179
0.0AlaXaa: 0.0 ± 0.0
Cys
0.68CysAla: 0.68 ± 0.099
0.084CysCys: 0.084 ± 0.033
0.585CysAsp: 0.585 ± 0.085
0.418CysGlu: 0.418 ± 0.061
0.394CysPhe: 0.394 ± 0.072
0.549CysGly: 0.549 ± 0.089
0.298CysHis: 0.298 ± 0.06
0.453CysIle: 0.453 ± 0.06
0.489CysLys: 0.489 ± 0.078
0.847CysLeu: 0.847 ± 0.105
0.334CysMet: 0.334 ± 0.066
0.453CysAsn: 0.453 ± 0.083
0.37CysPro: 0.37 ± 0.072
0.31CysGln: 0.31 ± 0.056
0.644CysArg: 0.644 ± 0.109
0.465CysSer: 0.465 ± 0.075
0.513CysThr: 0.513 ± 0.067
0.609CysVal: 0.609 ± 0.107
0.131CysTrp: 0.131 ± 0.047
0.382CysTyr: 0.382 ± 0.075
0.0CysXaa: 0.0 ± 0.0
Asp
5.263AspAla: 5.263 ± 0.254
0.465AspCys: 0.465 ± 0.073
4.034AspAsp: 4.034 ± 0.26
4.093AspGlu: 4.093 ± 0.268
2.375AspPhe: 2.375 ± 0.168
4.093AspGly: 4.093 ± 0.256
1.42AspHis: 1.42 ± 0.127
4.284AspIle: 4.284 ± 0.22
3.425AspLys: 3.425 ± 0.314
5.597AspLeu: 5.597 ± 0.27
1.623AspMet: 1.623 ± 0.144
3.091AspAsn: 3.091 ± 0.167
2.673AspPro: 2.673 ± 0.203
2.434AspGln: 2.434 ± 0.17
3.377AspArg: 3.377 ± 0.221
3.27AspSer: 3.27 ± 0.198
3.508AspThr: 3.508 ± 0.323
4.403AspVal: 4.403 ± 0.266
0.752AspTrp: 0.752 ± 0.1
2.637AspTyr: 2.637 ± 0.165
0.0AspXaa: 0.0 ± 0.0
Glu
4.785GluAla: 4.785 ± 0.29
0.501GluCys: 0.501 ± 0.085
3.27GluAsp: 3.27 ± 0.236
3.676GluGlu: 3.676 ± 0.241
2.303GluPhe: 2.303 ± 0.164
3.341GluGly: 3.341 ± 0.181
1.372GluHis: 1.372 ± 0.16
3.425GluIle: 3.425 ± 0.213
3.127GluLys: 3.127 ± 0.254
6.528GluLeu: 6.528 ± 0.305
1.85GluMet: 1.85 ± 0.135
2.232GluAsn: 2.232 ± 0.154
1.957GluPro: 1.957 ± 0.149
2.685GluGln: 2.685 ± 0.177
3.306GluArg: 3.306 ± 0.226
3.103GluSer: 3.103 ± 0.166
2.804GluThr: 2.804 ± 0.171
4.189GluVal: 4.189 ± 0.237
0.955GluTrp: 0.955 ± 0.108
2.076GluTyr: 2.076 ± 0.176
0.0GluXaa: 0.0 ± 0.0
Phe
2.661PheAla: 2.661 ± 0.169
0.37PheCys: 0.37 ± 0.078
2.828PheAsp: 2.828 ± 0.193
2.184PheGlu: 2.184 ± 0.169
1.253PhePhe: 1.253 ± 0.129
2.327PheGly: 2.327 ± 0.154
0.668PheHis: 0.668 ± 0.097
2.339PheIle: 2.339 ± 0.17
2.375PheLys: 2.375 ± 0.191
2.578PheLeu: 2.578 ± 0.182
1.014PheMet: 1.014 ± 0.11
2.649PheAsn: 2.649 ± 0.2
1.325PhePro: 1.325 ± 0.141
0.99PheGln: 0.99 ± 0.12
1.468PheArg: 1.468 ± 0.134
2.148PheSer: 2.148 ± 0.139
2.78PheThr: 2.78 ± 0.167
2.709PheVal: 2.709 ± 0.185
0.286PheTrp: 0.286 ± 0.061
1.372PheTyr: 1.372 ± 0.16
0.0PheXaa: 0.0 ± 0.0
Gly
5.287GlyAla: 5.287 ± 0.448
0.43GlyCys: 0.43 ± 0.064
5.012GlyAsp: 5.012 ± 0.668
3.986GlyGlu: 3.986 ± 0.251
2.291GlyPhe: 2.291 ± 0.166
5.943GlyGly: 5.943 ± 0.764
1.098GlyHis: 1.098 ± 0.116
4.034GlyIle: 4.034 ± 0.201
3.831GlyLys: 3.831 ± 0.223
5.68GlyLeu: 5.68 ± 0.283
2.411GlyMet: 2.411 ± 0.171
3.473GlyAsn: 3.473 ± 0.263
1.623GlyPro: 1.623 ± 0.191
2.506GlyGln: 2.506 ± 0.209
2.876GlyArg: 2.876 ± 0.232
4.403GlySer: 4.403 ± 0.274
4.105GlyThr: 4.105 ± 0.343
4.547GlyVal: 4.547 ± 0.266
1.122GlyTrp: 1.122 ± 0.115
3.103GlyTyr: 3.103 ± 0.197
0.0GlyXaa: 0.0 ± 0.0
His
1.527HisAla: 1.527 ± 0.165
0.286HisCys: 0.286 ± 0.062
1.492HisAsp: 1.492 ± 0.135
1.325HisGlu: 1.325 ± 0.138
0.835HisPhe: 0.835 ± 0.104
1.468HisGly: 1.468 ± 0.149
0.668HisHis: 0.668 ± 0.094
1.563HisIle: 1.563 ± 0.156
0.752HisLys: 0.752 ± 0.094
1.73HisLeu: 1.73 ± 0.146
0.668HisMet: 0.668 ± 0.104
0.967HisAsn: 0.967 ± 0.11
1.002HisPro: 1.002 ± 0.125
0.99HisGln: 0.99 ± 0.106
1.193HisArg: 1.193 ± 0.134
0.967HisSer: 0.967 ± 0.116
1.253HisThr: 1.253 ± 0.123
1.575HisVal: 1.575 ± 0.145
0.346HisTrp: 0.346 ± 0.062
1.038HisTyr: 1.038 ± 0.131
0.0HisXaa: 0.0 ± 0.0
Ile
5.31IleAla: 5.31 ± 0.245
0.453IleCys: 0.453 ± 0.071
4.368IleAsp: 4.368 ± 0.253
3.974IleGlu: 3.974 ± 0.239
1.635IlePhe: 1.635 ± 0.135
3.771IleGly: 3.771 ± 0.199
1.098IleHis: 1.098 ± 0.139
3.174IleIle: 3.174 ± 0.217
3.413IleLys: 3.413 ± 0.209
4.201IleLeu: 4.201 ± 0.251
1.48IleMet: 1.48 ± 0.115
3.687IleAsn: 3.687 ± 0.214
2.983IlePro: 2.983 ± 0.191
1.981IleGln: 1.981 ± 0.155
2.983IleArg: 2.983 ± 0.185
3.759IleSer: 3.759 ± 0.241
4.881IleThr: 4.881 ± 0.325
3.711IleVal: 3.711 ± 0.233
0.501IleTrp: 0.501 ± 0.072
1.838IleTyr: 1.838 ± 0.15
0.0IleXaa: 0.0 ± 0.0
Lys
5.167LysAla: 5.167 ± 0.288
0.358LysCys: 0.358 ± 0.072
3.186LysAsp: 3.186 ± 0.213
3.485LysGlu: 3.485 ± 0.249
1.838LysPhe: 1.838 ± 0.131
4.189LysGly: 4.189 ± 0.747
1.313LysHis: 1.313 ± 0.14
2.769LysIle: 2.769 ± 0.211
2.601LysLys: 2.601 ± 0.193
4.809LysLeu: 4.809 ± 0.317
1.527LysMet: 1.527 ± 0.129
2.112LysAsn: 2.112 ± 0.183
2.327LysPro: 2.327 ± 0.209
2.351LysGln: 2.351 ± 0.179
2.912LysArg: 2.912 ± 0.201
2.84LysSer: 2.84 ± 0.174
2.995LysThr: 2.995 ± 0.216
3.508LysVal: 3.508 ± 0.178
0.656LysTrp: 0.656 ± 0.102
2.196LysTyr: 2.196 ± 0.169
0.0LysXaa: 0.0 ± 0.0
Leu
7.041LeuAla: 7.041 ± 0.291
1.265LeuCys: 1.265 ± 0.134
5.287LeuAsp: 5.287 ± 0.255
4.833LeuGlu: 4.833 ± 0.242
3.091LeuPhe: 3.091 ± 0.183
5.513LeuGly: 5.513 ± 0.272
1.647LeuHis: 1.647 ± 0.12
5.0LeuIle: 5.0 ± 0.309
4.642LeuLys: 4.642 ± 0.261
7.077LeuLeu: 7.077 ± 0.344
2.458LeuMet: 2.458 ± 0.198
4.797LeuAsn: 4.797 ± 0.245
4.129LeuPro: 4.129 ± 0.221
3.616LeuGln: 3.616 ± 0.198
4.702LeuArg: 4.702 ± 0.273
6.814LeuSer: 6.814 ± 0.285
6.062LeuThr: 6.062 ± 0.277
6.456LeuVal: 6.456 ± 0.282
0.859LeuTrp: 0.859 ± 0.103
3.174LeuTyr: 3.174 ± 0.199
0.0LeuXaa: 0.0 ± 0.0
Met
2.446MetAla: 2.446 ± 0.144
0.167MetCys: 0.167 ± 0.044
1.492MetAsp: 1.492 ± 0.144
1.337MetGlu: 1.337 ± 0.133
1.146MetPhe: 1.146 ± 0.107
1.73MetGly: 1.73 ± 0.147
0.609MetHis: 0.609 ± 0.087
1.539MetIle: 1.539 ± 0.158
1.575MetLys: 1.575 ± 0.129
2.745MetLeu: 2.745 ± 0.182
1.038MetMet: 1.038 ± 0.137
1.432MetAsn: 1.432 ± 0.113
1.337MetPro: 1.337 ± 0.174
1.408MetGln: 1.408 ± 0.162
1.325MetArg: 1.325 ± 0.126
2.542MetSer: 2.542 ± 0.167
2.566MetThr: 2.566 ± 0.19
1.945MetVal: 1.945 ± 0.147
0.215MetTrp: 0.215 ± 0.05
1.11MetTyr: 1.11 ± 0.11
0.0MetXaa: 0.0 ± 0.0
Asn
4.069AsnAla: 4.069 ± 0.232
0.406AsnCys: 0.406 ± 0.062
3.091AsnAsp: 3.091 ± 0.207
2.59AsnGlu: 2.59 ± 0.157
1.599AsnPhe: 1.599 ± 0.114
4.045AsnGly: 4.045 ± 0.232
1.05AsnHis: 1.05 ± 0.101
2.828AsnIle: 2.828 ± 0.179
2.864AsnLys: 2.864 ± 0.221
4.248AsnLeu: 4.248 ± 0.188
1.492AsnMet: 1.492 ± 0.17
2.613AsnAsn: 2.613 ± 0.203
3.067AsnPro: 3.067 ± 0.19
1.957AsnGln: 1.957 ± 0.156
2.446AsnArg: 2.446 ± 0.181
2.685AsnSer: 2.685 ± 0.186
3.699AsnThr: 3.699 ± 0.18
3.449AsnVal: 3.449 ± 0.215
0.549AsnTrp: 0.549 ± 0.073
1.85AsnTyr: 1.85 ± 0.142
0.0AsnXaa: 0.0 ± 0.0
Pro
3.866ProAla: 3.866 ± 0.267
0.227ProCys: 0.227 ± 0.051
3.031ProAsp: 3.031 ± 0.2
2.995ProGlu: 2.995 ± 0.195
1.504ProPhe: 1.504 ± 0.15
2.78ProGly: 2.78 ± 0.19
0.895ProHis: 0.895 ± 0.124
2.59ProIle: 2.59 ± 0.202
2.064ProLys: 2.064 ± 0.155
3.532ProLeu: 3.532 ± 0.239
1.098ProMet: 1.098 ± 0.129
1.838ProAsn: 1.838 ± 0.156
1.635ProPro: 1.635 ± 0.182
2.029ProGln: 2.029 ± 0.305
1.659ProArg: 1.659 ± 0.161
2.578ProSer: 2.578 ± 0.174
3.019ProThr: 3.019 ± 0.199
3.628ProVal: 3.628 ± 0.221
0.525ProTrp: 0.525 ± 0.098
1.599ProTyr: 1.599 ± 0.144
0.0ProXaa: 0.0 ± 0.0
Gln
3.652GlnAla: 3.652 ± 0.274
0.394GlnCys: 0.394 ± 0.064
1.766GlnAsp: 1.766 ± 0.188
1.897GlnGlu: 1.897 ± 0.188
1.635GlnPhe: 1.635 ± 0.145
2.446GlnGly: 2.446 ± 0.281
1.205GlnHis: 1.205 ± 0.131
2.136GlnIle: 2.136 ± 0.151
1.611GlnLys: 1.611 ± 0.14
4.165GlnLeu: 4.165 ± 0.235
1.348GlnMet: 1.348 ± 0.232
1.635GlnAsn: 1.635 ± 0.156
2.243GlnPro: 2.243 ± 0.221
2.828GlnGln: 2.828 ± 0.453
2.22GlnArg: 2.22 ± 0.152
2.041GlnSer: 2.041 ± 0.152
2.399GlnThr: 2.399 ± 0.183
2.733GlnVal: 2.733 ± 0.208
0.549GlnTrp: 0.549 ± 0.078
1.921GlnTyr: 1.921 ± 0.16
0.0GlnXaa: 0.0 ± 0.0
Arg
3.723ArgAla: 3.723 ± 0.201
0.525ArgCys: 0.525 ± 0.085
2.852ArgAsp: 2.852 ± 0.201
3.091ArgGlu: 3.091 ± 0.212
2.458ArgPhe: 2.458 ± 0.147
2.769ArgGly: 2.769 ± 0.195
1.169ArgHis: 1.169 ± 0.119
3.019ArgIle: 3.019 ± 0.174
2.554ArgLys: 2.554 ± 0.183
5.119ArgLeu: 5.119 ± 0.279
1.456ArgMet: 1.456 ± 0.123
2.971ArgAsn: 2.971 ± 0.163
1.874ArgPro: 1.874 ± 0.141
2.029ArgGln: 2.029 ± 0.159
2.733ArgArg: 2.733 ± 0.198
2.757ArgSer: 2.757 ± 0.18
2.721ArgThr: 2.721 ± 0.19
3.306ArgVal: 3.306 ± 0.197
0.585ArgTrp: 0.585 ± 0.097
2.243ArgTyr: 2.243 ± 0.164
0.0ArgXaa: 0.0 ± 0.0
Ser
4.392SerAla: 4.392 ± 0.26
0.609SerCys: 0.609 ± 0.087
3.687SerAsp: 3.687 ± 0.23
3.031SerGlu: 3.031 ± 0.201
2.267SerPhe: 2.267 ± 0.161
4.224SerGly: 4.224 ± 0.288
1.158SerHis: 1.158 ± 0.129
3.664SerIle: 3.664 ± 0.229
3.21SerLys: 3.21 ± 0.2
5.43SerLeu: 5.43 ± 0.23
1.838SerMet: 1.838 ± 0.151
3.031SerAsn: 3.031 ± 0.188
2.566SerPro: 2.566 ± 0.195
2.315SerGln: 2.315 ± 0.176
2.792SerArg: 2.792 ± 0.207
3.497SerSer: 3.497 ± 0.287
4.344SerThr: 4.344 ± 0.285
4.594SerVal: 4.594 ± 0.274
0.704SerTrp: 0.704 ± 0.095
2.017SerTyr: 2.017 ± 0.153
0.0SerXaa: 0.0 ± 0.0
Thr
5.633ThrAla: 5.633 ± 0.223
0.43ThrCys: 0.43 ± 0.083
4.129ThrAsp: 4.129 ± 0.222
3.318ThrGlu: 3.318 ± 0.199
2.291ThrPhe: 2.291 ± 0.197
5.131ThrGly: 5.131 ± 0.427
1.551ThrHis: 1.551 ± 0.142
3.676ThrIle: 3.676 ± 0.222
3.019ThrLys: 3.019 ± 0.171
5.704ThrLeu: 5.704 ± 0.256
1.599ThrMet: 1.599 ± 0.146
3.067ThrAsn: 3.067 ± 0.227
3.843ThrPro: 3.843 ± 0.213
2.566ThrGln: 2.566 ± 0.197
3.174ThrArg: 3.174 ± 0.208
3.64ThrSer: 3.64 ± 0.216
4.427ThrThr: 4.427 ± 0.258
4.976ThrVal: 4.976 ± 0.247
0.692ThrTrp: 0.692 ± 0.092
2.721ThrTyr: 2.721 ± 0.225
0.0ThrXaa: 0.0 ± 0.0
Val
5.466ValAla: 5.466 ± 0.261
0.764ValCys: 0.764 ± 0.113
4.332ValAsp: 4.332 ± 0.251
3.962ValGlu: 3.962 ± 0.24
2.518ValPhe: 2.518 ± 0.184
4.547ValGly: 4.547 ± 0.258
1.42ValHis: 1.42 ± 0.13
4.773ValIle: 4.773 ± 0.267
4.403ValLys: 4.403 ± 0.254
5.74ValLeu: 5.74 ± 0.252
2.446ValMet: 2.446 ± 0.166
3.783ValAsn: 3.783 ± 0.186
2.888ValPro: 2.888 ± 0.191
2.279ValGln: 2.279 ± 0.146
3.413ValArg: 3.413 ± 0.208
4.642ValSer: 4.642 ± 0.252
5.275ValThr: 5.275 ± 0.271
6.265ValVal: 6.265 ± 0.313
0.752ValTrp: 0.752 ± 0.103
2.53ValTyr: 2.53 ± 0.206
0.0ValXaa: 0.0 ± 0.0
Trp
0.847TrpAla: 0.847 ± 0.104
0.072TrpCys: 0.072 ± 0.034
0.752TrpAsp: 0.752 ± 0.088
0.573TrpGlu: 0.573 ± 0.087
0.585TrpPhe: 0.585 ± 0.079
0.561TrpGly: 0.561 ± 0.08
0.346TrpHis: 0.346 ± 0.066
0.68TrpIle: 0.68 ± 0.099
0.513TrpLys: 0.513 ± 0.072
1.48TrpLeu: 1.48 ± 0.151
0.537TrpMet: 0.537 ± 0.072
0.501TrpAsn: 0.501 ± 0.073
0.322TrpPro: 0.322 ± 0.056
0.406TrpGln: 0.406 ± 0.072
0.68TrpArg: 0.68 ± 0.083
0.692TrpSer: 0.692 ± 0.113
0.8TrpThr: 0.8 ± 0.098
0.883TrpVal: 0.883 ± 0.099
0.155TrpTrp: 0.155 ± 0.037
0.549TrpTyr: 0.549 ± 0.074
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.852TyrAla: 2.852 ± 0.21
0.513TyrCys: 0.513 ± 0.076
2.47TyrAsp: 2.47 ± 0.204
1.981TyrGlu: 1.981 ± 0.163
1.134TyrPhe: 1.134 ± 0.107
2.637TyrGly: 2.637 ± 0.18
1.11TyrHis: 1.11 ± 0.123
1.981TyrIle: 1.981 ± 0.162
1.885TyrLys: 1.885 ± 0.136
3.759TyrLeu: 3.759 ± 0.235
0.811TyrMet: 0.811 ± 0.086
2.112TyrAsn: 2.112 ± 0.199
1.993TyrPro: 1.993 ± 0.21
1.802TyrGln: 1.802 ± 0.133
2.243TyrArg: 2.243 ± 0.147
2.029TyrSer: 2.029 ± 0.182
2.506TyrThr: 2.506 ± 0.168
2.661TyrVal: 2.661 ± 0.177
0.549TyrTrp: 0.549 ± 0.08
1.635TyrTyr: 1.635 ± 0.137
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 318 proteins (83799 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski