Wuhan Louse Fly Virus 10

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sigmavirus; Lousefly sigmavirus

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KKC0|A0A0B5KKC0_9RHAB Matrix protein OS=Wuhan Louse Fly Virus 10 OX=1608114 GN=M PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.31RR3 pKa = 11.84RR4 pKa = 11.84MSSNEE9 pKa = 3.83LKK11 pKa = 10.59DD12 pKa = 3.92LRR14 pKa = 11.84AGTEE18 pKa = 3.45WDD20 pKa = 3.78LPASKK25 pKa = 10.22LRR27 pKa = 11.84KK28 pKa = 8.82LQEE31 pKa = 4.13AGVLNRR37 pKa = 11.84IYY39 pKa = 10.15EE40 pKa = 4.09QQQTSTIDD48 pKa = 3.26PTIGLDD54 pKa = 3.6PSIMEE59 pKa = 4.31HH60 pKa = 7.54DD61 pKa = 4.19YY62 pKa = 11.83DD63 pKa = 4.23EE64 pKa = 4.79TDD66 pKa = 3.08HH67 pKa = 6.53TLSLGDD73 pKa = 3.72SLMRR77 pKa = 11.84SPDD80 pKa = 3.51YY81 pKa = 10.83LIPPEE86 pKa = 4.43DD87 pKa = 3.83KK88 pKa = 10.98EE89 pKa = 4.3EE90 pKa = 4.14EE91 pKa = 4.21EE92 pKa = 4.51TLSEE96 pKa = 4.19TSRR99 pKa = 11.84EE100 pKa = 4.19DD101 pKa = 3.46EE102 pKa = 4.25NSAKK106 pKa = 10.49YY107 pKa = 10.29PEE109 pKa = 4.57FEE111 pKa = 4.15GSVMEE116 pKa = 4.37TFQIDD121 pKa = 3.85VTSPHH126 pKa = 6.66HH127 pKa = 6.9LSDD130 pKa = 5.05PEE132 pKa = 4.19LLCRR136 pKa = 11.84KK137 pKa = 7.93LTHH140 pKa = 7.25LIKK143 pKa = 10.77LVGNGRR149 pKa = 11.84IKK151 pKa = 10.41PEE153 pKa = 3.71QVKK156 pKa = 9.36FVHH159 pKa = 6.64KK160 pKa = 10.63NVEE163 pKa = 4.04KK164 pKa = 10.45PNTKK168 pKa = 9.72IIQDD172 pKa = 3.78KK173 pKa = 10.7GDD175 pKa = 3.53NTIDD179 pKa = 3.48SLEE182 pKa = 4.21AKK184 pKa = 10.17EE185 pKa = 4.64NLTLNEE191 pKa = 3.92DD192 pKa = 3.48HH193 pKa = 7.24KK194 pKa = 10.29EE195 pKa = 4.07TRR197 pKa = 11.84EE198 pKa = 3.68RR199 pKa = 11.84SAQSKK204 pKa = 9.86RR205 pKa = 11.84ASQKK209 pKa = 9.88VDD211 pKa = 3.15NDD213 pKa = 3.17EE214 pKa = 4.39KK215 pKa = 11.0LYY217 pKa = 10.57EE218 pKa = 3.95SHH220 pKa = 7.44PFIKK224 pKa = 10.31RR225 pKa = 11.84IADD228 pKa = 3.62GVEE231 pKa = 3.81IPHH234 pKa = 6.72ILNPKK239 pKa = 8.35RR240 pKa = 11.84TLKK243 pKa = 9.93IDD245 pKa = 3.46KK246 pKa = 8.49EE247 pKa = 4.33HH248 pKa = 7.46PDD250 pKa = 3.42FNLIRR255 pKa = 11.84IKK257 pKa = 10.35EE258 pKa = 4.24VYY260 pKa = 9.1PKK262 pKa = 10.31YY263 pKa = 10.46HH264 pKa = 6.13QFPKK268 pKa = 10.76DD269 pKa = 3.22FAKK272 pKa = 10.63FLITIAQMSKK282 pKa = 9.54KK283 pKa = 8.42WDD285 pKa = 4.24FYY287 pKa = 10.57RR288 pKa = 11.84RR289 pKa = 11.84TYY291 pKa = 11.01NWVLFAEE298 pKa = 4.78SGMEE302 pKa = 3.97HH303 pKa = 6.93

Molecular weight: 35.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KRT4|A0A0B5KRT4_9RHAB Glycoprotein OS=Wuhan Louse Fly Virus 10 OX=1608114 GN=G PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 10.51NMKK5 pKa = 9.71KK6 pKa = 10.41SNRR9 pKa = 11.84GYY11 pKa = 10.21IEE13 pKa = 3.78MNRR16 pKa = 11.84LRR18 pKa = 11.84KK19 pKa = 9.35IPPKK23 pKa = 10.22LRR25 pKa = 11.84RR26 pKa = 11.84EE27 pKa = 3.94NGKK30 pKa = 9.53PSSSTGAAHH39 pKa = 6.89TSQVSDD45 pKa = 3.12SSQWTTVHH53 pKa = 5.42FTVPEE58 pKa = 4.23GTGSSRR64 pKa = 11.84RR65 pKa = 11.84VSLIKK70 pKa = 10.89GNWTVTGTLRR80 pKa = 11.84VSCDD84 pKa = 3.13KK85 pKa = 11.25NLTNWSMMEE94 pKa = 3.79RR95 pKa = 11.84TLEE98 pKa = 4.67HH99 pKa = 6.88ILDD102 pKa = 4.03VYY104 pKa = 10.4DD105 pKa = 3.79GNMVFKK111 pKa = 10.42PLILLFYY118 pKa = 9.84WILGVNLAPIPGPSNRR134 pKa = 11.84HH135 pKa = 4.55CWGVDD140 pKa = 3.65FGEE143 pKa = 4.86PISLNHH149 pKa = 6.93RR150 pKa = 11.84IEE152 pKa = 5.31LIGSQDD158 pKa = 3.01FGYY161 pKa = 10.82SKK163 pKa = 11.1NFASNFRR170 pKa = 11.84GHH172 pKa = 6.83KK173 pKa = 8.74FTVEE177 pKa = 3.37FNINFVHH184 pKa = 5.48TQRR187 pKa = 11.84RR188 pKa = 11.84TTSVVTIVNSQVPHH202 pKa = 5.87FKK204 pKa = 10.14DD205 pKa = 3.04RR206 pKa = 11.84RR207 pKa = 11.84RR208 pKa = 11.84FRR210 pKa = 11.84EE211 pKa = 4.04LLEE214 pKa = 4.46GSQISYY220 pKa = 10.56EE221 pKa = 4.12YY222 pKa = 10.3TEE224 pKa = 4.39EE225 pKa = 4.1EE226 pKa = 4.58GLVLTT231 pKa = 5.33

Molecular weight: 26.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3661

231

2146

732.2

84.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.851 ± 0.812

1.475 ± 0.309

4.725 ± 0.495

6.501 ± 0.646

4.562 ± 0.259

5.299 ± 0.465

3.114 ± 0.165

8.468 ± 0.631

7.02 ± 0.619

10.653 ± 0.788

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.021 ± 0.325

5.272 ± 0.48

4.753 ± 0.301

3.305 ± 0.218

4.807 ± 0.613

8.14 ± 0.28

5.763 ± 0.473

4.835 ± 0.345

1.694 ± 0.144

3.742 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski