Amino acid dipepetide frequency for Gordonia phage Cucurbita

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.538AlaAla: 10.538 ± 0.88
0.547AlaCys: 0.547 ± 0.153
6.911AlaAsp: 6.911 ± 0.424
6.603AlaGlu: 6.603 ± 0.576
2.942AlaPhe: 2.942 ± 0.374
7.938AlaGly: 7.938 ± 0.613
2.121AlaHis: 2.121 ± 0.361
4.379AlaIle: 4.379 ± 0.435
3.9AlaLys: 3.9 ± 0.489
8.04AlaLeu: 8.04 ± 0.501
2.532AlaMet: 2.532 ± 0.274
3.148AlaAsn: 3.148 ± 0.351
4.379AlaPro: 4.379 ± 0.45
4.619AlaGln: 4.619 ± 0.678
6.843AlaArg: 6.843 ± 0.677
4.619AlaSer: 4.619 ± 0.43
4.995AlaThr: 4.995 ± 0.495
7.219AlaVal: 7.219 ± 0.529
1.848AlaTrp: 1.848 ± 0.205
2.292AlaTyr: 2.292 ± 0.275
0.0AlaXaa: 0.0 ± 0.0
Cys
0.718CysAla: 0.718 ± 0.184
0.205CysCys: 0.205 ± 0.098
0.958CysAsp: 0.958 ± 0.244
0.718CysGlu: 0.718 ± 0.156
0.308CysPhe: 0.308 ± 0.089
1.129CysGly: 1.129 ± 0.264
0.342CysHis: 0.342 ± 0.125
0.479CysIle: 0.479 ± 0.126
0.513CysLys: 0.513 ± 0.123
0.513CysLeu: 0.513 ± 0.164
0.0CysMet: 0.0 ± 0.0
0.411CysAsn: 0.411 ± 0.129
0.547CysPro: 0.547 ± 0.153
0.479CysGln: 0.479 ± 0.137
0.684CysArg: 0.684 ± 0.163
0.445CysSer: 0.445 ± 0.13
0.547CysThr: 0.547 ± 0.134
0.718CysVal: 0.718 ± 0.169
0.137CysTrp: 0.137 ± 0.07
0.171CysTyr: 0.171 ± 0.097
0.0CysXaa: 0.0 ± 0.0
Asp
6.295AspAla: 6.295 ± 0.45
0.718AspCys: 0.718 ± 0.205
4.721AspAsp: 4.721 ± 0.529
4.687AspGlu: 4.687 ± 0.482
2.429AspPhe: 2.429 ± 0.244
5.987AspGly: 5.987 ± 0.478
1.574AspHis: 1.574 ± 0.202
3.421AspIle: 3.421 ± 0.367
2.532AspLys: 2.532 ± 0.308
5.782AspLeu: 5.782 ± 0.391
1.848AspMet: 1.848 ± 0.28
2.155AspAsn: 2.155 ± 0.335
4.311AspPro: 4.311 ± 0.403
2.155AspGln: 2.155 ± 0.297
5.474AspArg: 5.474 ± 0.513
2.806AspSer: 2.806 ± 0.283
4.516AspThr: 4.516 ± 0.423
5.269AspVal: 5.269 ± 0.375
1.471AspTrp: 1.471 ± 0.255
2.771AspTyr: 2.771 ± 0.317
0.0AspXaa: 0.0 ± 0.0
Glu
7.253GluAla: 7.253 ± 0.755
0.65GluCys: 0.65 ± 0.236
4.071GluAsp: 4.071 ± 0.423
4.243GluGlu: 4.243 ± 0.415
2.429GluPhe: 2.429 ± 0.261
4.585GluGly: 4.585 ± 0.294
1.3GluHis: 1.3 ± 0.229
4.14GluIle: 4.14 ± 0.399
2.6GluLys: 2.6 ± 0.329
6.466GluLeu: 6.466 ± 0.506
2.019GluMet: 2.019 ± 0.241
2.087GluAsn: 2.087 ± 0.232
2.84GluPro: 2.84 ± 0.547
2.737GluGln: 2.737 ± 0.314
4.414GluArg: 4.414 ± 0.378
3.353GluSer: 3.353 ± 0.367
3.661GluThr: 3.661 ± 0.353
4.345GluVal: 4.345 ± 0.476
2.053GluTrp: 2.053 ± 0.291
2.327GluTyr: 2.327 ± 0.279
0.0GluXaa: 0.0 ± 0.0
Phe
3.319PheAla: 3.319 ± 0.314
0.411PheCys: 0.411 ± 0.117
2.874PheAsp: 2.874 ± 0.325
2.327PheGlu: 2.327 ± 0.305
0.718PhePhe: 0.718 ± 0.141
2.19PheGly: 2.19 ± 0.321
0.718PheHis: 0.718 ± 0.124
1.334PheIle: 1.334 ± 0.216
1.232PheLys: 1.232 ± 0.196
2.155PheLeu: 2.155 ± 0.307
1.026PheMet: 1.026 ± 0.172
1.574PheAsn: 1.574 ± 0.218
1.471PhePro: 1.471 ± 0.223
0.718PheGln: 0.718 ± 0.178
1.54PheArg: 1.54 ± 0.222
2.121PheSer: 2.121 ± 0.25
2.292PheThr: 2.292 ± 0.279
2.327PheVal: 2.327 ± 0.39
0.753PheTrp: 0.753 ± 0.16
0.821PheTyr: 0.821 ± 0.129
0.0PheXaa: 0.0 ± 0.0
Gly
5.851GlyAla: 5.851 ± 0.544
0.89GlyCys: 0.89 ± 0.205
5.372GlyAsp: 5.372 ± 0.658
5.166GlyGlu: 5.166 ± 0.414
2.737GlyPhe: 2.737 ± 0.332
6.193GlyGly: 6.193 ± 0.613
1.916GlyHis: 1.916 ± 0.285
3.969GlyIle: 3.969 ± 0.349
3.558GlyLys: 3.558 ± 0.322
6.09GlyLeu: 6.09 ± 0.466
2.087GlyMet: 2.087 ± 0.241
2.669GlyAsn: 2.669 ± 0.328
3.764GlyPro: 3.764 ± 0.379
2.977GlyGln: 2.977 ± 0.349
4.961GlyArg: 4.961 ± 0.363
4.653GlySer: 4.653 ± 0.366
4.893GlyThr: 4.893 ± 0.424
6.603GlyVal: 6.603 ± 0.463
2.361GlyTrp: 2.361 ± 0.354
2.224GlyTyr: 2.224 ± 0.264
0.0GlyXaa: 0.0 ± 0.0
His
1.676HisAla: 1.676 ± 0.25
0.342HisCys: 0.342 ± 0.118
1.129HisAsp: 1.129 ± 0.223
1.505HisGlu: 1.505 ± 0.223
0.958HisPhe: 0.958 ± 0.177
2.121HisGly: 2.121 ± 0.309
0.513HisHis: 0.513 ± 0.131
1.026HisIle: 1.026 ± 0.196
0.65HisLys: 0.65 ± 0.142
1.984HisLeu: 1.984 ± 0.262
0.411HisMet: 0.411 ± 0.134
0.855HisAsn: 0.855 ± 0.17
1.437HisPro: 1.437 ± 0.248
0.821HisGln: 0.821 ± 0.169
1.676HisArg: 1.676 ± 0.249
1.334HisSer: 1.334 ± 0.185
1.437HisThr: 1.437 ± 0.229
1.848HisVal: 1.848 ± 0.276
0.411HisTrp: 0.411 ± 0.107
0.821HisTyr: 0.821 ± 0.195
0.0HisXaa: 0.0 ± 0.0
Ile
4.824IleAla: 4.824 ± 0.382
0.582IleCys: 0.582 ± 0.151
4.14IleAsp: 4.14 ± 0.432
3.729IleGlu: 3.729 ± 0.328
0.958IlePhe: 0.958 ± 0.165
3.25IleGly: 3.25 ± 0.314
1.095IleHis: 1.095 ± 0.18
2.087IleIle: 2.087 ± 0.339
2.327IleLys: 2.327 ± 0.302
3.079IleLeu: 3.079 ± 0.318
0.479IleMet: 0.479 ± 0.113
1.779IleAsn: 1.779 ± 0.297
2.463IlePro: 2.463 ± 0.326
1.745IleGln: 1.745 ± 0.252
3.9IleArg: 3.9 ± 0.391
2.908IleSer: 2.908 ± 0.384
3.216IleThr: 3.216 ± 0.341
3.011IleVal: 3.011 ± 0.376
1.026IleTrp: 1.026 ± 0.184
1.334IleTyr: 1.334 ± 0.233
0.0IleXaa: 0.0 ± 0.0
Lys
3.49LysAla: 3.49 ± 0.334
0.582LysCys: 0.582 ± 0.193
2.429LysAsp: 2.429 ± 0.294
2.224LysGlu: 2.224 ± 0.304
1.505LysPhe: 1.505 ± 0.276
3.079LysGly: 3.079 ± 0.352
0.958LysHis: 0.958 ± 0.2
1.608LysIle: 1.608 ± 0.208
2.155LysLys: 2.155 ± 0.302
3.627LysLeu: 3.627 ± 0.341
1.129LysMet: 1.129 ± 0.224
1.403LysAsn: 1.403 ± 0.208
2.395LysPro: 2.395 ± 0.269
1.266LysGln: 1.266 ± 0.226
2.977LysArg: 2.977 ± 0.313
2.292LysSer: 2.292 ± 0.282
1.984LysThr: 1.984 ± 0.295
3.832LysVal: 3.832 ± 0.386
0.718LysTrp: 0.718 ± 0.149
1.129LysTyr: 1.129 ± 0.214
0.0LysXaa: 0.0 ± 0.0
Leu
6.843LeuAla: 6.843 ± 0.695
0.855LeuCys: 0.855 ± 0.195
6.09LeuAsp: 6.09 ± 0.495
5.953LeuGlu: 5.953 ± 0.509
2.258LeuPhe: 2.258 ± 0.285
5.851LeuGly: 5.851 ± 0.489
1.745LeuHis: 1.745 ± 0.273
3.558LeuIle: 3.558 ± 0.264
2.669LeuLys: 2.669 ± 0.335
4.995LeuLeu: 4.995 ± 0.455
1.711LeuMet: 1.711 ± 0.255
3.387LeuAsn: 3.387 ± 0.339
3.661LeuPro: 3.661 ± 0.403
3.148LeuGln: 3.148 ± 0.354
5.44LeuArg: 5.44 ± 0.392
4.277LeuSer: 4.277 ± 0.38
4.79LeuThr: 4.79 ± 0.436
4.961LeuVal: 4.961 ± 0.472
1.266LeuTrp: 1.266 ± 0.2
1.984LeuTyr: 1.984 ± 0.232
0.0LeuXaa: 0.0 ± 0.0
Met
3.011MetAla: 3.011 ± 0.302
0.171MetCys: 0.171 ± 0.072
1.3MetAsp: 1.3 ± 0.223
1.163MetGlu: 1.163 ± 0.187
0.753MetPhe: 0.753 ± 0.135
1.574MetGly: 1.574 ± 0.23
0.684MetHis: 0.684 ± 0.191
1.061MetIle: 1.061 ± 0.214
0.855MetLys: 0.855 ± 0.144
1.711MetLeu: 1.711 ± 0.229
0.479MetMet: 0.479 ± 0.141
1.129MetAsn: 1.129 ± 0.167
1.403MetPro: 1.403 ± 0.194
0.924MetGln: 0.924 ± 0.171
1.848MetArg: 1.848 ± 0.248
2.669MetSer: 2.669 ± 0.272
2.155MetThr: 2.155 ± 0.296
1.574MetVal: 1.574 ± 0.265
0.308MetTrp: 0.308 ± 0.105
0.445MetTyr: 0.445 ± 0.138
0.0MetXaa: 0.0 ± 0.0
Asn
3.661AsnAla: 3.661 ± 0.391
0.547AsnCys: 0.547 ± 0.136
2.429AsnAsp: 2.429 ± 0.293
2.361AsnGlu: 2.361 ± 0.239
0.958AsnPhe: 0.958 ± 0.194
3.592AsnGly: 3.592 ± 0.334
0.958AsnHis: 0.958 ± 0.193
1.505AsnIle: 1.505 ± 0.237
1.471AsnLys: 1.471 ± 0.266
3.011AsnLeu: 3.011 ± 0.336
0.616AsnMet: 0.616 ± 0.176
1.095AsnAsn: 1.095 ± 0.23
2.019AsnPro: 2.019 ± 0.237
1.369AsnGln: 1.369 ± 0.23
2.429AsnArg: 2.429 ± 0.334
2.019AsnSer: 2.019 ± 0.264
2.087AsnThr: 2.087 ± 0.31
2.361AsnVal: 2.361 ± 0.289
0.616AsnTrp: 0.616 ± 0.115
0.958AsnTyr: 0.958 ± 0.221
0.0AsnXaa: 0.0 ± 0.0
Pro
5.337ProAla: 5.337 ± 0.439
0.342ProCys: 0.342 ± 0.106
3.661ProAsp: 3.661 ± 0.378
3.798ProGlu: 3.798 ± 0.473
1.848ProPhe: 1.848 ± 0.257
4.653ProGly: 4.653 ± 0.411
0.787ProHis: 0.787 ± 0.189
2.258ProIle: 2.258 ± 0.258
2.155ProLys: 2.155 ± 0.345
2.771ProLeu: 2.771 ± 0.332
1.026ProMet: 1.026 ± 0.185
1.745ProAsn: 1.745 ± 0.239
2.087ProPro: 2.087 ± 0.321
1.574ProGln: 1.574 ± 0.231
2.703ProArg: 2.703 ± 0.322
2.669ProSer: 2.669 ± 0.376
3.285ProThr: 3.285 ± 0.528
3.387ProVal: 3.387 ± 0.382
0.992ProTrp: 0.992 ± 0.162
1.574ProTyr: 1.574 ± 0.229
0.0ProXaa: 0.0 ± 0.0
Gln
3.49GlnAla: 3.49 ± 0.456
0.342GlnCys: 0.342 ± 0.116
1.916GlnAsp: 1.916 ± 0.258
2.19GlnGlu: 2.19 ± 0.308
1.061GlnPhe: 1.061 ± 0.173
2.634GlnGly: 2.634 ± 0.25
0.753GlnHis: 0.753 ± 0.188
1.95GlnIle: 1.95 ± 0.263
1.608GlnLys: 1.608 ± 0.329
3.764GlnLeu: 3.764 ± 0.428
1.266GlnMet: 1.266 ± 0.227
1.711GlnAsn: 1.711 ± 0.291
1.437GlnPro: 1.437 ± 0.21
1.711GlnGln: 1.711 ± 0.379
2.771GlnArg: 2.771 ± 0.376
2.053GlnSer: 2.053 ± 0.337
1.676GlnThr: 1.676 ± 0.283
2.292GlnVal: 2.292 ± 0.234
0.958GlnTrp: 0.958 ± 0.16
0.924GlnTyr: 0.924 ± 0.164
0.0GlnXaa: 0.0 ± 0.0
Arg
6.295ArgAla: 6.295 ± 0.515
0.684ArgCys: 0.684 ± 0.206
4.961ArgAsp: 4.961 ± 0.396
5.029ArgGlu: 5.029 ± 0.423
2.121ArgPhe: 2.121 ± 0.24
4.824ArgGly: 4.824 ± 0.413
1.916ArgHis: 1.916 ± 0.281
3.319ArgIle: 3.319 ± 0.3
3.216ArgLys: 3.216 ± 0.335
5.269ArgLeu: 5.269 ± 0.416
1.813ArgMet: 1.813 ± 0.237
2.669ArgAsn: 2.669 ± 0.253
2.771ArgPro: 2.771 ± 0.33
2.634ArgGln: 2.634 ± 0.283
6.022ArgArg: 6.022 ± 0.543
3.764ArgSer: 3.764 ± 0.362
3.661ArgThr: 3.661 ± 0.371
5.372ArgVal: 5.372 ± 0.406
1.608ArgTrp: 1.608 ± 0.231
2.19ArgTyr: 2.19 ± 0.268
0.0ArgXaa: 0.0 ± 0.0
Ser
5.919SerAla: 5.919 ± 0.479
0.513SerCys: 0.513 ± 0.154
3.935SerAsp: 3.935 ± 0.419
3.764SerGlu: 3.764 ± 0.315
1.711SerPhe: 1.711 ± 0.219
4.893SerGly: 4.893 ± 0.52
1.163SerHis: 1.163 ± 0.203
2.908SerIle: 2.908 ± 0.341
2.121SerLys: 2.121 ± 0.298
4.106SerLeu: 4.106 ± 0.353
1.813SerMet: 1.813 ± 0.21
1.916SerAsn: 1.916 ± 0.265
2.19SerPro: 2.19 ± 0.237
1.608SerGln: 1.608 ± 0.227
3.832SerArg: 3.832 ± 0.345
3.148SerSer: 3.148 ± 0.348
3.353SerThr: 3.353 ± 0.336
4.243SerVal: 4.243 ± 0.445
1.334SerTrp: 1.334 ± 0.208
1.984SerTyr: 1.984 ± 0.255
0.0SerXaa: 0.0 ± 0.0
Thr
6.295ThrAla: 6.295 ± 0.445
0.582ThrCys: 0.582 ± 0.156
4.174ThrAsp: 4.174 ± 0.375
4.174ThrGlu: 4.174 ± 0.295
1.984ThrPhe: 1.984 ± 0.238
4.961ThrGly: 4.961 ± 0.467
1.369ThrHis: 1.369 ± 0.195
3.148ThrIle: 3.148 ± 0.353
2.806ThrLys: 2.806 ± 0.37
3.832ThrLeu: 3.832 ± 0.281
1.3ThrMet: 1.3 ± 0.195
1.813ThrAsn: 1.813 ± 0.219
4.208ThrPro: 4.208 ± 0.456
1.882ThrGln: 1.882 ± 0.272
3.592ThrArg: 3.592 ± 0.365
3.866ThrSer: 3.866 ± 0.406
3.148ThrThr: 3.148 ± 0.392
3.969ThrVal: 3.969 ± 0.375
1.129ThrTrp: 1.129 ± 0.225
1.711ThrTyr: 1.711 ± 0.276
0.0ThrXaa: 0.0 ± 0.0
Val
7.219ValAla: 7.219 ± 0.551
0.65ValCys: 0.65 ± 0.175
5.816ValAsp: 5.816 ± 0.411
4.927ValGlu: 4.927 ± 0.505
2.634ValPhe: 2.634 ± 0.312
5.679ValGly: 5.679 ± 0.387
1.711ValHis: 1.711 ± 0.243
3.49ValIle: 3.49 ± 0.355
2.669ValLys: 2.669 ± 0.334
4.893ValLeu: 4.893 ± 0.466
2.224ValMet: 2.224 ± 0.234
2.292ValAsn: 2.292 ± 0.287
3.216ValPro: 3.216 ± 0.329
2.19ValGln: 2.19 ± 0.223
5.303ValArg: 5.303 ± 0.398
4.379ValSer: 4.379 ± 0.487
5.064ValThr: 5.064 ± 0.417
5.953ValVal: 5.953 ± 0.537
1.403ValTrp: 1.403 ± 0.192
1.95ValTyr: 1.95 ± 0.32
0.0ValXaa: 0.0 ± 0.0
Trp
1.779TrpAla: 1.779 ± 0.236
0.205TrpCys: 0.205 ± 0.089
1.642TrpAsp: 1.642 ± 0.178
1.403TrpGlu: 1.403 ± 0.207
0.718TrpPhe: 0.718 ± 0.167
1.437TrpGly: 1.437 ± 0.255
0.753TrpHis: 0.753 ± 0.154
1.197TrpIle: 1.197 ± 0.165
0.787TrpLys: 0.787 ± 0.18
1.608TrpLeu: 1.608 ± 0.239
0.787TrpMet: 0.787 ± 0.145
0.89TrpAsn: 0.89 ± 0.161
0.992TrpPro: 0.992 ± 0.179
0.89TrpGln: 0.89 ± 0.159
1.129TrpArg: 1.129 ± 0.228
1.266TrpSer: 1.266 ± 0.221
1.54TrpThr: 1.54 ± 0.207
1.608TrpVal: 1.608 ± 0.251
0.547TrpTrp: 0.547 ± 0.135
0.547TrpTyr: 0.547 ± 0.18
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.634TyrAla: 2.634 ± 0.273
0.205TyrCys: 0.205 ± 0.089
2.395TyrAsp: 2.395 ± 0.387
1.608TyrGlu: 1.608 ± 0.275
0.89TyrPhe: 0.89 ± 0.199
2.361TyrGly: 2.361 ± 0.223
0.582TyrHis: 0.582 ± 0.176
1.095TyrIle: 1.095 ± 0.212
0.958TyrLys: 0.958 ± 0.182
1.745TyrLeu: 1.745 ± 0.25
0.616TyrMet: 0.616 ± 0.164
1.334TyrAsn: 1.334 ± 0.226
1.061TyrPro: 1.061 ± 0.242
1.061TyrGln: 1.061 ± 0.189
2.6TyrArg: 2.6 ± 0.351
1.813TyrSer: 1.813 ± 0.252
1.642TyrThr: 1.642 ± 0.281
2.771TyrVal: 2.771 ± 0.399
0.787TyrTrp: 0.787 ± 0.156
0.582TyrTyr: 0.582 ± 0.137
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 178 proteins (29229 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski