Amino acid dipepetide frequency for Streptococcus phage Javan33

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.108AlaAla: 3.108 ± 0.739
0.274AlaCys: 0.274 ± 0.152
5.118AlaAsp: 5.118 ± 0.553
5.393AlaGlu: 5.393 ± 0.988
2.376AlaPhe: 2.376 ± 0.488
4.204AlaGly: 4.204 ± 0.709
0.457AlaHis: 0.457 ± 0.189
5.667AlaIle: 5.667 ± 1.216
5.301AlaLys: 5.301 ± 0.691
5.393AlaLeu: 5.393 ± 0.613
2.011AlaMet: 2.011 ± 0.375
3.565AlaAsn: 3.565 ± 0.714
1.005AlaPro: 1.005 ± 0.348
2.559AlaGln: 2.559 ± 0.404
3.565AlaArg: 3.565 ± 0.497
4.753AlaSer: 4.753 ± 0.812
3.747AlaThr: 3.747 ± 0.589
5.118AlaVal: 5.118 ± 0.918
1.097AlaTrp: 1.097 ± 0.33
2.925AlaTyr: 2.925 ± 0.434
0.0AlaXaa: 0.0 ± 0.0
Cys
0.366CysAla: 0.366 ± 0.169
0.0CysCys: 0.0 ± 0.0
0.366CysAsp: 0.366 ± 0.191
0.457CysGlu: 0.457 ± 0.203
0.183CysPhe: 0.183 ± 0.131
0.183CysGly: 0.183 ± 0.118
0.457CysHis: 0.457 ± 0.2
0.183CysIle: 0.183 ± 0.131
0.457CysLys: 0.457 ± 0.225
0.457CysLeu: 0.457 ± 0.224
0.0CysMet: 0.0 ± 0.0
0.366CysAsn: 0.366 ± 0.171
0.091CysPro: 0.091 ± 0.086
0.183CysGln: 0.183 ± 0.123
0.183CysArg: 0.183 ± 0.157
0.457CysSer: 0.457 ± 0.183
0.183CysThr: 0.183 ± 0.141
0.457CysVal: 0.457 ± 0.203
0.091CysTrp: 0.091 ± 0.091
0.457CysTyr: 0.457 ± 0.227
0.0CysXaa: 0.0 ± 0.0
Asp
2.925AspAla: 2.925 ± 0.522
0.183AspCys: 0.183 ± 0.127
4.936AspAsp: 4.936 ± 0.733
5.027AspGlu: 5.027 ± 0.63
3.199AspPhe: 3.199 ± 0.614
3.473AspGly: 3.473 ± 0.58
0.457AspHis: 0.457 ± 0.186
6.032AspIle: 6.032 ± 0.829
5.667AspLys: 5.667 ± 0.638
4.936AspLeu: 4.936 ± 0.608
1.919AspMet: 1.919 ± 0.376
3.656AspAsn: 3.656 ± 0.492
1.462AspPro: 1.462 ± 0.416
1.28AspGln: 1.28 ± 0.231
2.651AspArg: 2.651 ± 0.437
3.747AspSer: 3.747 ± 0.69
2.925AspThr: 2.925 ± 0.425
3.108AspVal: 3.108 ± 0.375
1.28AspTrp: 1.28 ± 0.392
2.925AspTyr: 2.925 ± 0.718
0.0AspXaa: 0.0 ± 0.0
Glu
5.667GluAla: 5.667 ± 0.643
0.457GluCys: 0.457 ± 0.24
3.199GluAsp: 3.199 ± 0.601
6.215GluGlu: 6.215 ± 0.946
2.742GluPhe: 2.742 ± 0.566
3.656GluGly: 3.656 ± 0.728
1.097GluHis: 1.097 ± 0.294
5.667GluIle: 5.667 ± 0.715
6.398GluLys: 6.398 ± 1.199
8.957GluLeu: 8.957 ± 1.033
2.011GluMet: 2.011 ± 0.51
4.57GluAsn: 4.57 ± 0.759
1.645GluPro: 1.645 ± 0.383
3.016GluGln: 3.016 ± 0.587
3.29GluArg: 3.29 ± 0.668
4.479GluSer: 4.479 ± 0.527
6.307GluThr: 6.307 ± 0.692
5.027GluVal: 5.027 ± 0.604
1.097GluTrp: 1.097 ± 0.24
1.919GluTyr: 1.919 ± 0.41
0.0GluXaa: 0.0 ± 0.0
Phe
1.645PheAla: 1.645 ± 0.432
0.183PheCys: 0.183 ± 0.125
4.296PheAsp: 4.296 ± 0.673
4.113PheGlu: 4.113 ± 0.691
1.188PhePhe: 1.188 ± 0.352
2.285PheGly: 2.285 ± 0.429
0.823PheHis: 0.823 ± 0.241
2.468PheIle: 2.468 ± 0.52
2.925PheLys: 2.925 ± 0.571
2.742PheLeu: 2.742 ± 0.612
1.28PheMet: 1.28 ± 0.354
3.016PheAsn: 3.016 ± 0.567
1.005PhePro: 1.005 ± 0.433
1.28PheGln: 1.28 ± 0.315
1.645PheArg: 1.645 ± 0.393
2.742PheSer: 2.742 ± 0.462
2.376PheThr: 2.376 ± 0.465
1.462PheVal: 1.462 ± 0.327
0.274PheTrp: 0.274 ± 0.167
1.462PheTyr: 1.462 ± 0.348
0.0PheXaa: 0.0 ± 0.0
Gly
4.387GlyAla: 4.387 ± 1.065
0.457GlyCys: 0.457 ± 0.185
2.833GlyAsp: 2.833 ± 0.597
3.93GlyGlu: 3.93 ± 0.702
3.199GlyPhe: 3.199 ± 0.689
4.022GlyGly: 4.022 ± 0.724
1.462GlyHis: 1.462 ± 0.387
4.844GlyIle: 4.844 ± 0.507
5.118GlyLys: 5.118 ± 0.653
4.661GlyLeu: 4.661 ± 0.621
1.554GlyMet: 1.554 ± 0.54
3.473GlyAsn: 3.473 ± 0.705
0.274GlyPro: 0.274 ± 0.126
2.376GlyGln: 2.376 ± 0.518
2.833GlyArg: 2.833 ± 0.699
3.839GlySer: 3.839 ± 0.659
3.747GlyThr: 3.747 ± 0.778
3.473GlyVal: 3.473 ± 0.633
1.188GlyTrp: 1.188 ± 0.321
2.925GlyTyr: 2.925 ± 0.492
0.0GlyXaa: 0.0 ± 0.0
His
0.64HisAla: 0.64 ± 0.232
0.183HisCys: 0.183 ± 0.134
1.462HisAsp: 1.462 ± 0.417
1.371HisGlu: 1.371 ± 0.386
0.64HisPhe: 0.64 ± 0.226
0.823HisGly: 0.823 ± 0.27
0.091HisHis: 0.091 ± 0.097
1.645HisIle: 1.645 ± 0.335
1.005HisLys: 1.005 ± 0.393
1.371HisLeu: 1.371 ± 0.349
0.183HisMet: 0.183 ± 0.141
0.914HisAsn: 0.914 ± 0.334
0.457HisPro: 0.457 ± 0.2
0.548HisGln: 0.548 ± 0.216
0.366HisArg: 0.366 ± 0.215
0.914HisSer: 0.914 ± 0.274
1.188HisThr: 1.188 ± 0.37
0.731HisVal: 0.731 ± 0.192
0.091HisTrp: 0.091 ± 0.082
1.462HisTyr: 1.462 ± 0.452
0.0HisXaa: 0.0 ± 0.0
Ile
5.575IleAla: 5.575 ± 0.966
0.731IleCys: 0.731 ± 0.354
5.575IleAsp: 5.575 ± 0.701
6.032IleGlu: 6.032 ± 0.876
2.559IlePhe: 2.559 ± 0.5
3.747IleGly: 3.747 ± 0.747
1.28IleHis: 1.28 ± 0.323
3.93IleIle: 3.93 ± 0.78
6.398IleLys: 6.398 ± 0.692
5.667IleLeu: 5.667 ± 0.763
1.371IleMet: 1.371 ± 0.344
4.296IleAsn: 4.296 ± 0.617
2.011IlePro: 2.011 ± 0.388
2.833IleGln: 2.833 ± 0.479
3.108IleArg: 3.108 ± 0.489
4.113IleSer: 4.113 ± 0.464
5.027IleThr: 5.027 ± 0.689
3.473IleVal: 3.473 ± 0.609
0.64IleTrp: 0.64 ± 0.29
2.376IleTyr: 2.376 ± 0.562
0.0IleXaa: 0.0 ± 0.0
Lys
6.855LysAla: 6.855 ± 0.779
0.183LysCys: 0.183 ± 0.129
4.022LysAsp: 4.022 ± 0.82
7.312LysGlu: 7.312 ± 0.911
2.833LysPhe: 2.833 ± 0.439
4.936LysGly: 4.936 ± 0.626
1.097LysHis: 1.097 ± 0.295
5.484LysIle: 5.484 ± 0.645
6.764LysLys: 6.764 ± 0.926
6.764LysLeu: 6.764 ± 0.866
2.376LysMet: 2.376 ± 0.532
4.661LysAsn: 4.661 ± 0.75
1.737LysPro: 1.737 ± 0.395
3.199LysGln: 3.199 ± 0.449
2.742LysArg: 2.742 ± 0.613
6.032LysSer: 6.032 ± 0.87
5.85LysThr: 5.85 ± 0.755
4.753LysVal: 4.753 ± 0.669
0.64LysTrp: 0.64 ± 0.223
3.29LysTyr: 3.29 ± 0.774
0.0LysXaa: 0.0 ± 0.0
Leu
5.85LeuAla: 5.85 ± 0.698
0.366LeuCys: 0.366 ± 0.194
5.301LeuAsp: 5.301 ± 0.546
7.403LeuGlu: 7.403 ± 0.872
3.747LeuPhe: 3.747 ± 0.603
5.301LeuGly: 5.301 ± 0.623
1.645LeuHis: 1.645 ± 0.402
5.85LeuIle: 5.85 ± 0.769
7.86LeuLys: 7.86 ± 0.888
5.667LeuLeu: 5.667 ± 0.659
2.285LeuMet: 2.285 ± 0.539
4.844LeuAsn: 4.844 ± 0.585
2.376LeuPro: 2.376 ± 0.468
2.285LeuGln: 2.285 ± 0.509
4.936LeuArg: 4.936 ± 0.58
7.038LeuSer: 7.038 ± 0.787
5.758LeuThr: 5.758 ± 0.723
3.747LeuVal: 3.747 ± 0.665
0.823LeuTrp: 0.823 ± 0.385
3.199LeuTyr: 3.199 ± 0.538
0.0LeuXaa: 0.0 ± 0.0
Met
1.371MetAla: 1.371 ± 0.275
0.274MetCys: 0.274 ± 0.198
1.737MetAsp: 1.737 ± 0.499
1.737MetGlu: 1.737 ± 0.342
0.548MetPhe: 0.548 ± 0.28
1.371MetGly: 1.371 ± 0.267
0.457MetHis: 0.457 ± 0.218
1.005MetIle: 1.005 ± 0.322
3.108MetLys: 3.108 ± 0.625
2.011MetLeu: 2.011 ± 0.406
0.457MetMet: 0.457 ± 0.214
1.645MetAsn: 1.645 ± 0.411
1.188MetPro: 1.188 ± 0.332
0.366MetGln: 0.366 ± 0.178
0.731MetArg: 0.731 ± 0.24
1.737MetSer: 1.737 ± 0.507
2.468MetThr: 2.468 ± 0.655
1.188MetVal: 1.188 ± 0.341
0.183MetTrp: 0.183 ± 0.127
0.274MetTyr: 0.274 ± 0.182
0.0MetXaa: 0.0 ± 0.0
Asn
4.661AsnAla: 4.661 ± 0.832
0.548AsnCys: 0.548 ± 0.244
2.925AsnAsp: 2.925 ± 0.644
3.93AsnGlu: 3.93 ± 0.524
2.925AsnPhe: 2.925 ± 0.541
4.753AsnGly: 4.753 ± 0.893
1.188AsnHis: 1.188 ± 0.345
3.565AsnIle: 3.565 ± 0.61
3.29AsnLys: 3.29 ± 0.472
5.301AsnLeu: 5.301 ± 0.559
1.097AsnMet: 1.097 ± 0.352
2.833AsnAsn: 2.833 ± 0.461
2.285AsnPro: 2.285 ± 0.412
2.651AsnGln: 2.651 ± 0.49
3.473AsnArg: 3.473 ± 0.591
3.473AsnSer: 3.473 ± 0.659
2.559AsnThr: 2.559 ± 0.445
3.382AsnVal: 3.382 ± 0.725
0.823AsnTrp: 0.823 ± 0.314
2.468AsnTyr: 2.468 ± 0.554
0.0AsnXaa: 0.0 ± 0.0
Pro
1.188ProAla: 1.188 ± 0.274
0.274ProCys: 0.274 ± 0.182
2.194ProAsp: 2.194 ± 0.402
1.28ProGlu: 1.28 ± 0.426
0.64ProPhe: 0.64 ± 0.259
0.823ProGly: 0.823 ± 0.27
0.64ProHis: 0.64 ± 0.215
2.468ProIle: 2.468 ± 0.594
2.102ProLys: 2.102 ± 0.52
2.559ProLeu: 2.559 ± 0.489
0.457ProMet: 0.457 ± 0.214
1.828ProAsn: 1.828 ± 0.384
0.457ProPro: 0.457 ± 0.22
1.188ProGln: 1.188 ± 0.27
1.28ProArg: 1.28 ± 0.357
2.102ProSer: 2.102 ± 0.573
1.645ProThr: 1.645 ± 0.356
1.828ProVal: 1.828 ± 0.324
0.274ProTrp: 0.274 ± 0.152
0.64ProTyr: 0.64 ± 0.203
0.0ProXaa: 0.0 ± 0.0
Gln
4.022GlnAla: 4.022 ± 0.441
0.183GlnCys: 0.183 ± 0.123
1.371GlnAsp: 1.371 ± 0.363
2.285GlnGlu: 2.285 ± 0.465
1.188GlnPhe: 1.188 ± 0.344
2.651GlnGly: 2.651 ± 0.534
0.457GlnHis: 0.457 ± 0.245
2.285GlnIle: 2.285 ± 0.449
2.925GlnLys: 2.925 ± 0.551
4.936GlnLeu: 4.936 ± 0.881
0.914GlnMet: 0.914 ± 0.263
1.645GlnAsn: 1.645 ± 0.303
1.097GlnPro: 1.097 ± 0.414
1.554GlnGln: 1.554 ± 0.49
2.742GlnArg: 2.742 ± 0.443
1.828GlnSer: 1.828 ± 0.49
1.645GlnThr: 1.645 ± 0.366
2.011GlnVal: 2.011 ± 0.409
0.274GlnTrp: 0.274 ± 0.156
1.371GlnTyr: 1.371 ± 0.309
0.0GlnXaa: 0.0 ± 0.0
Arg
2.925ArgAla: 2.925 ± 0.576
0.457ArgCys: 0.457 ± 0.224
2.559ArgAsp: 2.559 ± 0.431
3.473ArgGlu: 3.473 ± 0.755
1.462ArgPhe: 1.462 ± 0.381
2.742ArgGly: 2.742 ± 0.63
1.005ArgHis: 1.005 ± 0.318
3.108ArgIle: 3.108 ± 0.505
4.296ArgLys: 4.296 ± 0.699
4.296ArgLeu: 4.296 ± 0.568
0.64ArgMet: 0.64 ± 0.211
1.737ArgAsn: 1.737 ± 0.349
2.102ArgPro: 2.102 ± 0.564
2.194ArgGln: 2.194 ± 0.391
2.285ArgArg: 2.285 ± 0.648
2.468ArgSer: 2.468 ± 0.476
2.742ArgThr: 2.742 ± 0.725
4.022ArgVal: 4.022 ± 0.595
0.457ArgTrp: 0.457 ± 0.205
2.285ArgTyr: 2.285 ± 0.477
0.0ArgXaa: 0.0 ± 0.0
Ser
4.661SerAla: 4.661 ± 0.786
0.183SerCys: 0.183 ± 0.116
3.382SerAsp: 3.382 ± 0.507
4.479SerGlu: 4.479 ± 0.573
3.565SerPhe: 3.565 ± 0.812
4.204SerGly: 4.204 ± 0.967
1.097SerHis: 1.097 ± 0.29
3.93SerIle: 3.93 ± 0.554
4.204SerLys: 4.204 ± 0.543
5.575SerLeu: 5.575 ± 0.666
1.097SerMet: 1.097 ± 0.262
4.844SerAsn: 4.844 ± 0.605
1.645SerPro: 1.645 ± 0.431
3.29SerGln: 3.29 ± 0.572
3.016SerArg: 3.016 ± 0.639
5.21SerSer: 5.21 ± 1.063
4.296SerThr: 4.296 ± 0.816
3.382SerVal: 3.382 ± 0.563
0.457SerTrp: 0.457 ± 0.194
2.559SerTyr: 2.559 ± 0.703
0.0SerXaa: 0.0 ± 0.0
Thr
4.479ThrAla: 4.479 ± 0.754
0.366ThrCys: 0.366 ± 0.192
3.747ThrAsp: 3.747 ± 0.541
4.844ThrGlu: 4.844 ± 0.683
2.102ThrPhe: 2.102 ± 0.372
4.844ThrGly: 4.844 ± 0.71
0.64ThrHis: 0.64 ± 0.208
5.393ThrIle: 5.393 ± 0.637
5.393ThrLys: 5.393 ± 0.499
5.575ThrLeu: 5.575 ± 0.785
1.005ThrMet: 1.005 ± 0.273
3.199ThrAsn: 3.199 ± 0.622
1.919ThrPro: 1.919 ± 0.489
1.828ThrGln: 1.828 ± 0.365
2.559ThrArg: 2.559 ± 0.536
3.382ThrSer: 3.382 ± 0.995
4.113ThrThr: 4.113 ± 0.802
6.032ThrVal: 6.032 ± 0.694
0.914ThrTrp: 0.914 ± 0.355
2.559ThrTyr: 2.559 ± 0.466
0.0ThrXaa: 0.0 ± 0.0
Val
3.565ValAla: 3.565 ± 0.525
0.091ValCys: 0.091 ± 0.092
3.747ValAsp: 3.747 ± 0.596
4.113ValGlu: 4.113 ± 0.625
1.737ValPhe: 1.737 ± 0.538
3.565ValGly: 3.565 ± 0.767
0.823ValHis: 0.823 ± 0.28
4.479ValIle: 4.479 ± 0.625
4.661ValLys: 4.661 ± 0.658
3.565ValLeu: 3.565 ± 0.541
1.919ValMet: 1.919 ± 0.355
3.656ValAsn: 3.656 ± 0.595
1.554ValPro: 1.554 ± 0.361
1.919ValGln: 1.919 ± 0.496
2.559ValArg: 2.559 ± 0.487
4.204ValSer: 4.204 ± 0.584
5.484ValThr: 5.484 ± 0.798
3.473ValVal: 3.473 ± 0.653
1.097ValTrp: 1.097 ± 0.326
2.376ValTyr: 2.376 ± 0.47
0.0ValXaa: 0.0 ± 0.0
Trp
0.914TrpAla: 0.914 ± 0.302
0.091TrpCys: 0.091 ± 0.095
0.366TrpAsp: 0.366 ± 0.163
0.64TrpGlu: 0.64 ± 0.217
0.548TrpPhe: 0.548 ± 0.213
0.914TrpGly: 0.914 ± 0.362
0.183TrpHis: 0.183 ± 0.12
0.731TrpIle: 0.731 ± 0.242
0.64TrpLys: 0.64 ± 0.278
1.462TrpLeu: 1.462 ± 0.312
0.366TrpMet: 0.366 ± 0.2
0.914TrpAsn: 0.914 ± 0.271
0.091TrpPro: 0.091 ± 0.075
0.548TrpGln: 0.548 ± 0.232
0.731TrpArg: 0.731 ± 0.22
1.097TrpSer: 1.097 ± 0.471
0.914TrpThr: 0.914 ± 0.317
0.457TrpVal: 0.457 ± 0.196
0.0TrpTrp: 0.0 ± 0.0
0.457TrpTyr: 0.457 ± 0.16
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.833TyrAla: 2.833 ± 0.576
0.091TyrCys: 0.091 ± 0.089
2.651TyrAsp: 2.651 ± 0.489
3.382TyrGlu: 3.382 ± 0.64
1.828TyrPhe: 1.828 ± 0.418
2.102TyrGly: 2.102 ± 0.54
0.731TyrHis: 0.731 ± 0.307
2.011TyrIle: 2.011 ± 0.469
3.016TyrLys: 3.016 ± 0.577
4.387TyrLeu: 4.387 ± 0.608
0.823TyrMet: 0.823 ± 0.333
2.468TyrAsn: 2.468 ± 0.564
1.462TyrPro: 1.462 ± 0.431
2.285TyrGln: 2.285 ± 0.466
2.559TyrArg: 2.559 ± 0.611
1.462TyrSer: 1.462 ± 0.388
1.919TyrThr: 1.919 ± 0.434
1.554TyrVal: 1.554 ± 0.389
0.366TyrTrp: 0.366 ± 0.187
2.102TyrTyr: 2.102 ± 0.473
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (10942 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski